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The histopathological evaluation of the effect of combined graft and platelet rich fibrin implementation on healing of bone defects
1180
21st ICOMS 2013—Abstracts: Oral Papers
tors, cells or gene constructions. Considering some limitations of the first two we focused our efforts on making of gene-activated bone graft (GABG). Methods: We have developed several variants of GABGs consisting of scaffold (collagen/hydroxyapapite and xenogenic deproteinized bone matrix) and DNA plasmids with gene encoding vascular endothelial growth factor (VEGF). These gene constructions have high angiogenic activity critically significant for reparative osteogenesis. We incubated GABGs with cell cultures (multipotent mesenchymal stromal cells) and measured the expression level of VEGF in cultural medium. Control groups were the same cells incubated with scaffolds only and without any materials as well. Also we estimated GABGs efficacy in vivo. We have performed symmetric critical-size (10 mm) defects of both parietal bones of rabbits. GABGs were implanted into right defects, while scaffolds without plasmids – into left ones. In a row of cases two-cassette DNA plasmids additionally consisting of GFP gene were used for GABG making that allowed to detect the cells transfection in vivo. The results were assessed on 15, 30, 45, 60, 90, 120 days by CT scanning, histochemical, immunohistochemical and histomorphometric analyses. Results: We have shown the increase of VEGF expression by MMSC in case of their incubation with GABGs. Cells transfection in vivo by GABGs plasmids was detected and that resulted to more pronounced angiogenesis and reparative osteogenesis in all time points in experimental groups unlike controls. Conclusions: GABGs are effective for bone defects repair that allowed us to start the clinical trials. Key words gene-activated bone graft; plasmid VEGF; osteogenesis http://dx.doi.org/10.1016/j.ijom.2013.07.045 T2.OR002 The histopathological evaluation of the effect of combined graft and platelet rich fibrin implementation on healing of bone defects T. Develi 1,∗ , S.S. Soydan 1 , K. Araz 2 , B. Bayram 3 , E. Kübanya 1 , D. Bacanl´y 1 1
Fellow Professor 3 Assistant Professor 2
The purpose of this study was to evaluate and compare the effects of Platelet Rich Fibrin (PRF) usage combined with graft and as a membrane on healing of bone defects. For that purpose, four defects were constituted in each parietal bone of 14 Vienna rabbits. First defect was untreated as control (Group A), graft (HA/TCP) and collagen membrane were used in second defect (Group B), HA/TCP and PRF membrane were used in third defect (Group C) and combined HA/TCP – PRF graft and collagen membrane were used in fourth defect (Group D). All samples were sacrificed at post-operative 4th week (n: 7) and 8th week (n: 7) periods. Bone defect healing were evaluated histopathologically. It is found that there is a statistically significant difference between four groups in new bone generation for 4 and 8 weekly periods(p: 0.042 vs p: 0.015). At both periods, new bone generation is the highest in Group C, and; Group D, Group B and Group A come after sequentially. As a conclusion, highest new bone formation was determined in group which PRF membrane were used when compared the other groups both at 4th and 8th weeks.
It is shown that PRF is a successful autologenous alternative to collagen membrane. http://dx.doi.org/10.1016/j.ijom.2013.07.046 T2.OR003 Development of mandibular reconstruction device made of titanium fiber scaffold M. Hirota ∗ , I. Sato, T. Ozawa, T. Iwai, M. Kioi, K. Mitsudo, I. Tohnai Yokohama City University, Japan Background and objectives: Mandibular reconstruction after tumour ablative surgery is essential for returning QOL of oral cancer survivours. But bone reconstruction surgery puts a severe strain on the patient. To date, the author reported that titanium fibre mesh scaffold had favour biocompatibility and high bone formation ability that was enhanced by hydroxyapatite coating. In the present study, the author described the development of new mandibular reconstruction device with the titanium fibre mesh scaffold. Methods: Mandibular reconstruction materials made of titanium fibre mesh scaffold were made with or without hydroxiapatite (HA) coating. HA coating was performed with molecular precursor method that the scaffold was soaked into the Ca and diphosphate solution and heated at 600◦ for 2 h after the soaking. For surgery, adult female Japanese white rabbits were used. A part of the mandibular bone was completely removed, and the defect was reconstructed by the titanium fibre mesh scaffold. The materials was fixed with titanium plate. Twenty-one weeks after surgery, the 5 coating and 5 non-coating animals were sacrificed and the reconstructed part was evaluated with computed tomographic (CT) and histological analysis. Mann–Whitney U-test was performed for statistical analysis. Results: CT analysis revealed that the amount of new bone outside of the HA-coating titanium fibre scaffold was significantly higher than that of the untreated titanium fibre scaffold. Histological analysis revealed that the amount of new bone inside of the HA-coating titanium fibre scaffold was significantly higher than that of the untreated titanium fiber scaffold. Conculusion: In hydroxyaatite coating titanium fibre mesh scaffold, newly formed bone were seen in and out of the materials, and the defect was completely restored with newly formed bone, suggesting that titanium fibre mesh was powerful materials for mandibular reconstruction device by hydroxyapatite coating. http://dx.doi.org/10.1016/j.ijom.2013.07.047 T2.OR004 Translational research of HAp/agarose composite gel (HAp gel) used as a bone regenerative biomechanical material in jawbone defects S. Iwai 1,∗ , H. Shimizu 1 , A. Takeshita 1 , M. Akashi 2 , Y. Yura 1 1
Department of Oral and Maxillofacial Surgery II, Graduate School of Dentistry, Osaka University, Japan 2 Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Japan Background and objective: We have reported that HAp/agarose composite gel (HAp gel) is useful as a bone grafting material for bone defects. We previously investigated
21st ICOMS 2013—Abstracts: Oral Papers
tors, cells or gene constructions. Considering some limitations of the first two we focused our efforts on making of gene-activated bone graft (GABG). Methods: We have developed several variants of GABGs consisting of scaffold (collagen/hydroxyapapite and xenogenic deproteinized bone matrix) and DNA plasmids with gene encoding vascular endothelial growth factor (VEGF). These gene constructions have high angiogenic activity critically significant for reparative osteogenesis. We incubated GABGs with cell cultures (multipotent mesenchymal stromal cells) and measured the expression level of VEGF in cultural medium. Control groups were the same cells incubated with scaffolds only and without any materials as well. Also we estimated GABGs efficacy in vivo. We have performed symmetric critical-size (10 mm) defects of both parietal bones of rabbits. GABGs were implanted into right defects, while scaffolds without plasmids – into left ones. In a row of cases two-cassette DNA plasmids additionally consisting of GFP gene were used for GABG making that allowed to detect the cells transfection in vivo. The results were assessed on 15, 30, 45, 60, 90, 120 days by CT scanning, histochemical, immunohistochemical and histomorphometric analyses. Results: We have shown the increase of VEGF expression by MMSC in case of their incubation with GABGs. Cells transfection in vivo by GABGs plasmids was detected and that resulted to more pronounced angiogenesis and reparative osteogenesis in all time points in experimental groups unlike controls. Conclusions: GABGs are effective for bone defects repair that allowed us to start the clinical trials. Key words gene-activated bone graft; plasmid VEGF; osteogenesis http://dx.doi.org/10.1016/j.ijom.2013.07.045 T2.OR002 The histopathological evaluation of the effect of combined graft and platelet rich fibrin implementation on healing of bone defects T. Develi 1,∗ , S.S. Soydan 1 , K. Araz 2 , B. Bayram 3 , E. Kübanya 1 , D. Bacanl´y 1 1
Fellow Professor 3 Assistant Professor 2
The purpose of this study was to evaluate and compare the effects of Platelet Rich Fibrin (PRF) usage combined with graft and as a membrane on healing of bone defects. For that purpose, four defects were constituted in each parietal bone of 14 Vienna rabbits. First defect was untreated as control (Group A), graft (HA/TCP) and collagen membrane were used in second defect (Group B), HA/TCP and PRF membrane were used in third defect (Group C) and combined HA/TCP – PRF graft and collagen membrane were used in fourth defect (Group D). All samples were sacrificed at post-operative 4th week (n: 7) and 8th week (n: 7) periods. Bone defect healing were evaluated histopathologically. It is found that there is a statistically significant difference between four groups in new bone generation for 4 and 8 weekly periods(p: 0.042 vs p: 0.015). At both periods, new bone generation is the highest in Group C, and; Group D, Group B and Group A come after sequentially. As a conclusion, highest new bone formation was determined in group which PRF membrane were used when compared the other groups both at 4th and 8th weeks.
It is shown that PRF is a successful autologenous alternative to collagen membrane. http://dx.doi.org/10.1016/j.ijom.2013.07.046 T2.OR003 Development of mandibular reconstruction device made of titanium fiber scaffold M. Hirota ∗ , I. Sato, T. Ozawa, T. Iwai, M. Kioi, K. Mitsudo, I. Tohnai Yokohama City University, Japan Background and objectives: Mandibular reconstruction after tumour ablative surgery is essential for returning QOL of oral cancer survivours. But bone reconstruction surgery puts a severe strain on the patient. To date, the author reported that titanium fibre mesh scaffold had favour biocompatibility and high bone formation ability that was enhanced by hydroxyapatite coating. In the present study, the author described the development of new mandibular reconstruction device with the titanium fibre mesh scaffold. Methods: Mandibular reconstruction materials made of titanium fibre mesh scaffold were made with or without hydroxiapatite (HA) coating. HA coating was performed with molecular precursor method that the scaffold was soaked into the Ca and diphosphate solution and heated at 600◦ for 2 h after the soaking. For surgery, adult female Japanese white rabbits were used. A part of the mandibular bone was completely removed, and the defect was reconstructed by the titanium fibre mesh scaffold. The materials was fixed with titanium plate. Twenty-one weeks after surgery, the 5 coating and 5 non-coating animals were sacrificed and the reconstructed part was evaluated with computed tomographic (CT) and histological analysis. Mann–Whitney U-test was performed for statistical analysis. Results: CT analysis revealed that the amount of new bone outside of the HA-coating titanium fibre scaffold was significantly higher than that of the untreated titanium fibre scaffold. Histological analysis revealed that the amount of new bone inside of the HA-coating titanium fibre scaffold was significantly higher than that of the untreated titanium fiber scaffold. Conculusion: In hydroxyaatite coating titanium fibre mesh scaffold, newly formed bone were seen in and out of the materials, and the defect was completely restored with newly formed bone, suggesting that titanium fibre mesh was powerful materials for mandibular reconstruction device by hydroxyapatite coating. http://dx.doi.org/10.1016/j.ijom.2013.07.047 T2.OR004 Translational research of HAp/agarose composite gel (HAp gel) used as a bone regenerative biomechanical material in jawbone defects S. Iwai 1,∗ , H. Shimizu 1 , A. Takeshita 1 , M. Akashi 2 , Y. Yura 1 1
Department of Oral and Maxillofacial Surgery II, Graduate School of Dentistry, Osaka University, Japan 2 Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Japan Background and objective: We have reported that HAp/agarose composite gel (HAp gel) is useful as a bone grafting material for bone defects. We previously investigated