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The human asialoglycoprotein receptor: A specific target for autoimmune reactions in chronic liver diseases
246
EARLY ANTI-HCV SEROCONVWSION IN POST-TRANSFUSION HEPATITIS (PTH) A.Taggern.A.Alberti~,G.ReF.Tremolada. C.Casarin”. aldi) Dept.Exp.and Clin.H~Jicine,Catanzarti; * Inst. mica1 Hedicine,Padua;‘Inst.of Virology.tlilan; 5 Inst.of Clinical Hedicine,Sassari,ltaly;
lJ.Treid-el, H.L&. T.PuraUs, M.Msns, G.HeB, E.FleiscWr, K.-H. Nzyfz zun E&&Y-&& l.D?pma_rentof Mxlicina, l&= wsity of Mainz, m, Fffi
lt was reoortd that anti-WV seroconversion occur late during acute PTH and that only 202 of cases become anti-HCV positive during the first month. We have retrospectively evaluated the antibody to HCV by Elisa test (Ortho) in sera of a large serie of open-heart surgery pts (82 cases) who developed PTH during a prospective study. Overall 74 pts (9CZ) showed anti-IN3 seroconversio: after a mean of 7 weeks (range 2-49) from hepatitis onset and of 14.5 (4.7-55) from blood transfusion. About 502 oE cases~seroco&rted within 30 daysfrom hepatitis onset. All these reactivities were confil: med by recombinant immunoblot assay (RIBA.Ortho). The rate of anti-WV positivity during acute phase did not differ in resolved cases (802) and in those with chronic evolution (92X). Houcver during a follow-up (mean 6 years;range l-9) a disappearance of anti-WV was more frequently observed in patients who resolved (502) than in these with chronic evolu tion (9.52. p=O.OO!). In these latter no difference were noted in relation to histology ranging from CI’H to liver cirrhosis. In conclusion HCV is responsible for the majority of Plli cases and during acute phase anti-HCV seroconversion may occur et variable time-interval from onset of disease, but in at Least 50% of cases occurs early. This may be due to en higher sensibil:ty of Elisa anti-WV assay than RIA.
248
247 CORRELATIONBETWEENDETECTION OF HBV DNA IN SERUM BY PCR AND HBV A@ IN PBMC OF CHRONIC HEPATITIS
PATIENTS
WITHOUT
NATRIOPEPTIDE (ANP)-INDUCED HYPERNATRIURESIS IN PRE-ASClTIC ClRRHOTlCSDURlNG PROLONGED RECLJWENCY
HBs ANTIOENRMIA.
Trcvisani, H. Bernard,. A. Gasbarrinl P. AnF. rJreone. c. Cursaro. d. Baraldini. G. Gasbarrini. Patologia He&a I, University of Bologna, Italy.
C. TrCoa. F. Laurenr. C. Pichoud Zoulim. I. Baeinskb INSERM b!$??&!$?$e$ Thomas, 69424 LYON Cedex 03. France
Pre-ascitic cirrhotics show an exaggerated natriur&s when Kept recumbent (I). In order to detect the mechanism promoting this phenomenon, filtered load (Fw.I. tubular rc_iectlon fraction (TRFuJ and a.--. daily renal excretion (GNaV) of sodium, ~and”~lasma aldosterone (PA) and ANP (mean Of 2 Values OVCP 24h) were determined in 10 patients 7 health controls, kept recumbent for 2dh with a diet providing 40 mmol Na/day. ANP was determined in 5 controls and 5 patients. L~N,V of cirrhotics was markedly higher than in eontrok (t34?14 vs 87?9 mmol, pt0.02), in spite of reduced FNa (14.2 vs 19.0 mmol/min, pt0.02) and was due to a strikingly increased TRFNa (0.70?0.06% “s 0.32?0.03, ptO.001). PA did not differ in cirrhotics and contPOk (68fll vs 7728 pg/ml), while ANP was h&her in patients (106?12 Vs 60? 10 pg/ml, pt0.05). Log PA and log UNaV were correlated only in controls w:-0.68, o.op>o.o5). In controls and patients taKen together AW was correlated with loa uI.V (r=o.sc, pto.oot) and TRFNa (r-0.76. p
Hepatitis cases previously designated non-A non-B hepatitis on serological criteria including lack of detectable sernm and liver HBs Ag have been related to HBV on the basis of HBV DNA hybridization positlvity in serum and/or PBMC (Peripheral Blood Mononuclear Cells). Chimpanzee transmission as well as molecular biological studies have confnmed the possible existence of HBs Ag negative variants. In this study we have attempted to correlatein a series of 40 histologically documented chronic hepatitis cases the detection of HBV DNA in serum taking advantage of the increased sensitivity of PCR with that of HBV markers in serum and PBMC (ABBOTT RIA) and anti HCV serology (Grtho EIA): 22 cases (55 410)were HBV DNA positive by PCR and 16 (40 %) for HBV antibodies in serum, 15 (37.5 %) for HBs Ag and/or HBc/e Ag in PBMC. 23 (57.5 %) reacted for anti HCV. PCR detection of HBV DNA sequences correlated with that of HBV markers in PBMC (~~0.05) but neither with that of serum HBV antibodies nor with that of anti HCV. These data indicate that detection of HBV DNA sequences in serum by PCR cannot be dismissed as merely non specific. On the conuary, it should be used together with other investigations including HBV Ag testing in PBMC to further clarify the exact role of different hepatotropic viruses in the etiology of chronic hepatitis.
562
EARLY ANTI-HCV SEROCONVWSION IN POST-TRANSFUSION HEPATITIS (PTH) A.Taggern.A.Alberti~,G.ReF.Tremolada. C.Casarin”. aldi) Dept.Exp.and Clin.H~Jicine,Catanzarti; * Inst. mica1 Hedicine,Padua;‘Inst.of Virology.tlilan; 5 Inst.of Clinical Hedicine,Sassari,ltaly;
lJ.Treid-el, H.L&. T.PuraUs, M.Msns, G.HeB, E.FleiscWr, K.-H. Nzyfz zun E&&Y-&& l.D?pma_rentof Mxlicina, l&= wsity of Mainz, m, Fffi
lt was reoortd that anti-WV seroconversion occur late during acute PTH and that only 202 of cases become anti-HCV positive during the first month. We have retrospectively evaluated the antibody to HCV by Elisa test (Ortho) in sera of a large serie of open-heart surgery pts (82 cases) who developed PTH during a prospective study. Overall 74 pts (9CZ) showed anti-IN3 seroconversio: after a mean of 7 weeks (range 2-49) from hepatitis onset and of 14.5 (4.7-55) from blood transfusion. About 502 oE cases~seroco&rted within 30 daysfrom hepatitis onset. All these reactivities were confil: med by recombinant immunoblot assay (RIBA.Ortho). The rate of anti-WV positivity during acute phase did not differ in resolved cases (802) and in those with chronic evolution (92X). Houcver during a follow-up (mean 6 years;range l-9) a disappearance of anti-WV was more frequently observed in patients who resolved (502) than in these with chronic evolu tion (9.52. p=O.OO!). In these latter no difference were noted in relation to histology ranging from CI’H to liver cirrhosis. In conclusion HCV is responsible for the majority of Plli cases and during acute phase anti-HCV seroconversion may occur et variable time-interval from onset of disease, but in at Least 50% of cases occurs early. This may be due to en higher sensibil:ty of Elisa anti-WV assay than RIA.
248
247 CORRELATIONBETWEENDETECTION OF HBV DNA IN SERUM BY PCR AND HBV A@ IN PBMC OF CHRONIC HEPATITIS
PATIENTS
WITHOUT
NATRIOPEPTIDE (ANP)-INDUCED HYPERNATRIURESIS IN PRE-ASClTIC ClRRHOTlCSDURlNG PROLONGED RECLJWENCY
HBs ANTIOENRMIA.
Trcvisani, H. Bernard,. A. Gasbarrinl P. AnF. rJreone. c. Cursaro. d. Baraldini. G. Gasbarrini. Patologia He&a I, University of Bologna, Italy.
C. TrCoa. F. Laurenr. C. Pichoud Zoulim. I. Baeinskb INSERM b!$??&!$?$e$ Thomas, 69424 LYON Cedex 03. France
Pre-ascitic cirrhotics show an exaggerated natriur&s when Kept recumbent (I). In order to detect the mechanism promoting this phenomenon, filtered load (Fw.I. tubular rc_iectlon fraction (TRFuJ and a.--. daily renal excretion (GNaV) of sodium, ~and”~lasma aldosterone (PA) and ANP (mean Of 2 Values OVCP 24h) were determined in 10 patients 7 health controls, kept recumbent for 2dh with a diet providing 40 mmol Na/day. ANP was determined in 5 controls and 5 patients. L~N,V of cirrhotics was markedly higher than in eontrok (t34?14 vs 87?9 mmol, pt0.02), in spite of reduced FNa (14.2 vs 19.0 mmol/min, pt0.02) and was due to a strikingly increased TRFNa (0.70?0.06% “s 0.32?0.03, ptO.001). PA did not differ in cirrhotics and contPOk (68fll vs 7728 pg/ml), while ANP was h&her in patients (106?12 Vs 60? 10 pg/ml, pt0.05). Log PA and log UNaV were correlated only in controls w:-0.68, o.op>o.o5). In controls and patients taKen together AW was correlated with loa uI.V (r=o.sc, pto.oot) and TRFNa (r-0.76. p
Hepatitis cases previously designated non-A non-B hepatitis on serological criteria including lack of detectable sernm and liver HBs Ag have been related to HBV on the basis of HBV DNA hybridization positlvity in serum and/or PBMC (Peripheral Blood Mononuclear Cells). Chimpanzee transmission as well as molecular biological studies have confnmed the possible existence of HBs Ag negative variants. In this study we have attempted to correlatein a series of 40 histologically documented chronic hepatitis cases the detection of HBV DNA in serum taking advantage of the increased sensitivity of PCR with that of HBV markers in serum and PBMC (ABBOTT RIA) and anti HCV serology (Grtho EIA): 22 cases (55 410)were HBV DNA positive by PCR and 16 (40 %) for HBV antibodies in serum, 15 (37.5 %) for HBs Ag and/or HBc/e Ag in PBMC. 23 (57.5 %) reacted for anti HCV. PCR detection of HBV DNA sequences correlated with that of HBV markers in PBMC (~~0.05) but neither with that of serum HBV antibodies nor with that of anti HCV. These data indicate that detection of HBV DNA sequences in serum by PCR cannot be dismissed as merely non specific. On the conuary, it should be used together with other investigations including HBV Ag testing in PBMC to further clarify the exact role of different hepatotropic viruses in the etiology of chronic hepatitis.
562