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The human cytomegalovirus is associated with ischemic stroke and cerebral hemorrhage in a Chinese population
S80
Abstracts
siRNA-3 groups were higher and siRNA-3 was the highest at protein level by western blot assay, while no distinctive change was found between siRNA-con treated NRK-52E and blank control group. The concentrations of l-dopa uptake from different time points showed that compared with siRNA-con transfected group and blank control group, siRNA-interference group was lower and there was obvious differences in concentration- time trends between them while no significant difference in siRNA-con group and blank control group (Blank). Conclusion: The concentrations of l-dopa uptake from different time points decreased and concentration-time trends were obviously different after specifically silencing the slc7a8 expression. The results reveal the potential functions of slc7a8 and indicate that slc7a8 might be a new pathogenic gene of hypertension.
doi:10.1016/j.ijcard.2011.08.733 0108 The human cytomegalovirus is associated with ischemic stroke and cerebral hemorrhage in a Chinese population YONGQIN WANG, XINGYU WANG, JUN CAI Beijing Hypertension League Institute, Beijing, China Chaoyang Hospital, Beijing, China Objective: Stroke is a neurological deficit of cerebrovascular cause that persists beyond 24 h or is interrupted by death within 24 h. Human cytomegalovirus (HCMV) has been implicated in several cardiovascular disorders, including atherosclerosis, coronary heart disease, and cardiac transplant arteriopathy. However, little is known about the roles and regulation of HCMV infection in patients with stroke. Methods: The real time PCR and ELISA were performed in the present study. Human plasma samples were isolated from 200 stroke patients and 200 controls. All participants belonged to the Stroke Hypertension Investigation in Genetics (SHINING) study. Results: Seropositivity of HCMV was higher in 200 stroke patients compared with 200 controls (55.0% vs 23.5%, P b 0.0001). The presence of HCMV DNA increased the risk of stroke in Chinese population, the unadjusted OR = 3.98; 95%CI, 2.59 to 6.11; P b 0.0001. In the subtype of ischemic stroke, the unadjusted OR = 4.01; 95%CI, 2.57 to 6.24; P b 0.0001. And the unadjusted OR = 3.80; 95%CI, 1.64 to 8.78; P = 0.0018 in cerebral hemorrhage. The positive rate of HCMV DNA also increased the risk of ischemic stroke and cerebral hemorrhage after adjusting for age, sex, BMI, hypertension and smoking. Ischemic stroke (adjusted OR = 4.07; 95%CI, 2.52 to 6.32; P b 0.0001). Cerebral hemorrhage (adjusted OR = 3.88; 95%CI, 1.61 to 9.36; P = 0.0026). Conclusions: This is the first report demonstrating a link between HCMV DNA replication and ischemic stroke and cerebral hemorrhage. These findings may provide important insights toward diagnosis, screening, and pathogenesis of stroke.
doi:10.1016/j.ijcard.2011.08.734 0110 Association between adiponectin gene rs266729 polymorphism and salt sensitivity JIANJUN MU, GUANJI WU, YU CAO, DAN WANG, WEIHUA GAO Department of Cardiology, the First Affiliated Hospital of Medical College, Xian Jiaotong University, Xian 710061, Shanxi, China Objectives: To investigate the genotype distribution of adiponectin gene rs266729 polymorphism in salt-sensitive adults, and to study
the association between adiponectin gene rs266729 polymorphism and salt sensitivity. Methods: All subjects whom we have intervented in 2004 are Han peoples of Shanxi Province. 342 normotensive volunteers (including 56 families), aged 16 to 60, were selected to undergo a baseline survey, a low-sodium diet (51.3 mmol/day) for 7 days, a high-sodium diet (307.8 mmol/day) for an additional 7 days. All subjects were classified as salt sensitive group (SS, 130 subjects) or non-salt sensitive group (NSS, 212 subjects) on the basis of their mean arterial blood pressure (MAP) increase more than 10% at the end of the high-sodium phase compared with the low-sodium phase. Fasting blood samples were taken on the first day of baseline and on the sixth day of the three intervention phases. The DNA was extracted with ReadyAmpTM genomic DNA purification system. SNP rs266729 polymorphism was analyzed by ligase detection reaction-polymerase chain reaction (LDR-PCR). The LDR-PCR results were analyzed by FBAT5.5 software. Results: The prevalence of SS is 38%. There are 3 families salt-sensitive detection rate more than 75%, 7 families between 50% and 75%, 40 families between 25% and 50%, 6 families between 0 and 25%. SNP rs266729 (−11,377C/G) in adiponectin gene promoter region was significantly associated with salt sensitivity (Z = 2.320, P = 0.020325) and the reactivity of blood pressure to potassium (Z = 2.325, P = 0.020049). Conclusion: The SNP rs266729 (−11,377C/G) in adiponectin gene promoter region may contribute to the genetic risk for salt sensitivity and the reactivity of blood pressure to potassium. doi:10.1016/j.ijcard.2011.08.735 0161 Research on kidney aging and its associated gene among Uygur people in Hotan, Xinjiang XINJUAN XU, SUHUA LI, YULAN CHEN, ZHULEPIYA SIMAYI, XIAOHUI LIANG Department of Hypertension, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China Methods: Through case–control and Snapshot genotyping methods, studies were made on the distributions of polymorphisms and haplotypes in the Klotho gene at rs9527025, rs9536314, rs1207568 and rs564481 gene loci, and in the P66shc gene at rs8191981 and rs4845401 gene loci among clinically healthy Uygur volunteers being the control and people older than 90 as the long-lived group and on the association of their variations with the longevity and renal function aging among long-lived people. Results: (1) There was no statistical difference of the focus genotype and allele frequency distributions in Klotho and P66shc genes between the control and long-lived group. (2) The detected gene loci mutation in Klotho and P66shc genes focus was not statistically related with the longevity of Uygur through correlation analysis (P N 0.05). (3) There was no statistical difference of Klotho and P66shc genotype, allele and haplotypes frequency distributions between two groups of the normal renal function (eGFR ≥ 90 (ml/min/1.73m2) and the renal dysfunction (eGFR b 90 (ml/min/1.73m2) in the longevity group, divided by the decline of renal function (PN0.05). (4) The Klotho and P66shc gene polymorphisms were not statistically correlated with the renal decline of long-lived Uygur in a correlation analysis. (5) The combined effect of polymorphism of P66shc gene at rs8191981 and klotho gene at rs9536314 was statistically correlated with renal function decline among long-lived Uygur, showed in a analysis of interaction of P66shc and klotho genes on the renal function decline of the long-lived people (R = 0.082, P = 0.008). Conclusion: No evidence showed that the polymorphisms of P66shc and klotho genes were vulnerable genetic factors for the longevity and renal decline of the long-lived Uygur in Hotan. The combined effect of polymorphism of P66shc gene at rs8191981 and
Abstracts
siRNA-3 groups were higher and siRNA-3 was the highest at protein level by western blot assay, while no distinctive change was found between siRNA-con treated NRK-52E and blank control group. The concentrations of l-dopa uptake from different time points showed that compared with siRNA-con transfected group and blank control group, siRNA-interference group was lower and there was obvious differences in concentration- time trends between them while no significant difference in siRNA-con group and blank control group (Blank). Conclusion: The concentrations of l-dopa uptake from different time points decreased and concentration-time trends were obviously different after specifically silencing the slc7a8 expression. The results reveal the potential functions of slc7a8 and indicate that slc7a8 might be a new pathogenic gene of hypertension.
doi:10.1016/j.ijcard.2011.08.733 0108 The human cytomegalovirus is associated with ischemic stroke and cerebral hemorrhage in a Chinese population YONGQIN WANG, XINGYU WANG, JUN CAI Beijing Hypertension League Institute, Beijing, China Chaoyang Hospital, Beijing, China Objective: Stroke is a neurological deficit of cerebrovascular cause that persists beyond 24 h or is interrupted by death within 24 h. Human cytomegalovirus (HCMV) has been implicated in several cardiovascular disorders, including atherosclerosis, coronary heart disease, and cardiac transplant arteriopathy. However, little is known about the roles and regulation of HCMV infection in patients with stroke. Methods: The real time PCR and ELISA were performed in the present study. Human plasma samples were isolated from 200 stroke patients and 200 controls. All participants belonged to the Stroke Hypertension Investigation in Genetics (SHINING) study. Results: Seropositivity of HCMV was higher in 200 stroke patients compared with 200 controls (55.0% vs 23.5%, P b 0.0001). The presence of HCMV DNA increased the risk of stroke in Chinese population, the unadjusted OR = 3.98; 95%CI, 2.59 to 6.11; P b 0.0001. In the subtype of ischemic stroke, the unadjusted OR = 4.01; 95%CI, 2.57 to 6.24; P b 0.0001. And the unadjusted OR = 3.80; 95%CI, 1.64 to 8.78; P = 0.0018 in cerebral hemorrhage. The positive rate of HCMV DNA also increased the risk of ischemic stroke and cerebral hemorrhage after adjusting for age, sex, BMI, hypertension and smoking. Ischemic stroke (adjusted OR = 4.07; 95%CI, 2.52 to 6.32; P b 0.0001). Cerebral hemorrhage (adjusted OR = 3.88; 95%CI, 1.61 to 9.36; P = 0.0026). Conclusions: This is the first report demonstrating a link between HCMV DNA replication and ischemic stroke and cerebral hemorrhage. These findings may provide important insights toward diagnosis, screening, and pathogenesis of stroke.
doi:10.1016/j.ijcard.2011.08.734 0110 Association between adiponectin gene rs266729 polymorphism and salt sensitivity JIANJUN MU, GUANJI WU, YU CAO, DAN WANG, WEIHUA GAO Department of Cardiology, the First Affiliated Hospital of Medical College, Xian Jiaotong University, Xian 710061, Shanxi, China Objectives: To investigate the genotype distribution of adiponectin gene rs266729 polymorphism in salt-sensitive adults, and to study
the association between adiponectin gene rs266729 polymorphism and salt sensitivity. Methods: All subjects whom we have intervented in 2004 are Han peoples of Shanxi Province. 342 normotensive volunteers (including 56 families), aged 16 to 60, were selected to undergo a baseline survey, a low-sodium diet (51.3 mmol/day) for 7 days, a high-sodium diet (307.8 mmol/day) for an additional 7 days. All subjects were classified as salt sensitive group (SS, 130 subjects) or non-salt sensitive group (NSS, 212 subjects) on the basis of their mean arterial blood pressure (MAP) increase more than 10% at the end of the high-sodium phase compared with the low-sodium phase. Fasting blood samples were taken on the first day of baseline and on the sixth day of the three intervention phases. The DNA was extracted with ReadyAmpTM genomic DNA purification system. SNP rs266729 polymorphism was analyzed by ligase detection reaction-polymerase chain reaction (LDR-PCR). The LDR-PCR results were analyzed by FBAT5.5 software. Results: The prevalence of SS is 38%. There are 3 families salt-sensitive detection rate more than 75%, 7 families between 50% and 75%, 40 families between 25% and 50%, 6 families between 0 and 25%. SNP rs266729 (−11,377C/G) in adiponectin gene promoter region was significantly associated with salt sensitivity (Z = 2.320, P = 0.020325) and the reactivity of blood pressure to potassium (Z = 2.325, P = 0.020049). Conclusion: The SNP rs266729 (−11,377C/G) in adiponectin gene promoter region may contribute to the genetic risk for salt sensitivity and the reactivity of blood pressure to potassium. doi:10.1016/j.ijcard.2011.08.735 0161 Research on kidney aging and its associated gene among Uygur people in Hotan, Xinjiang XINJUAN XU, SUHUA LI, YULAN CHEN, ZHULEPIYA SIMAYI, XIAOHUI LIANG Department of Hypertension, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China Methods: Through case–control and Snapshot genotyping methods, studies were made on the distributions of polymorphisms and haplotypes in the Klotho gene at rs9527025, rs9536314, rs1207568 and rs564481 gene loci, and in the P66shc gene at rs8191981 and rs4845401 gene loci among clinically healthy Uygur volunteers being the control and people older than 90 as the long-lived group and on the association of their variations with the longevity and renal function aging among long-lived people. Results: (1) There was no statistical difference of the focus genotype and allele frequency distributions in Klotho and P66shc genes between the control and long-lived group. (2) The detected gene loci mutation in Klotho and P66shc genes focus was not statistically related with the longevity of Uygur through correlation analysis (P N 0.05). (3) There was no statistical difference of Klotho and P66shc genotype, allele and haplotypes frequency distributions between two groups of the normal renal function (eGFR ≥ 90 (ml/min/1.73m2) and the renal dysfunction (eGFR b 90 (ml/min/1.73m2) in the longevity group, divided by the decline of renal function (PN0.05). (4) The Klotho and P66shc gene polymorphisms were not statistically correlated with the renal decline of long-lived Uygur in a correlation analysis. (5) The combined effect of polymorphism of P66shc gene at rs8191981 and klotho gene at rs9536314 was statistically correlated with renal function decline among long-lived Uygur, showed in a analysis of interaction of P66shc and klotho genes on the renal function decline of the long-lived people (R = 0.082, P = 0.008). Conclusion: No evidence showed that the polymorphisms of P66shc and klotho genes were vulnerable genetic factors for the longevity and renal decline of the long-lived Uygur in Hotan. The combined effect of polymorphism of P66shc gene at rs8191981 and