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The immunohistochemical reactivity of an anti-epithelial monoclonal antibody (b-12) in meningiomas
Acta histochem. 87, 77 - 79 (1989) VEB Gustav Fischer Verlag Jena
Institute of Pathology (Head: Prof. Dr. R. WARZOK), Department of Medicine, Emst-Moritz-Amdt-University, Greifswald, GDR
The immunohistochemical reactivity of an anti-epithelial monoclonal antibody (b-12) in meningiomas By HANS.-JOACHIM TERPE and MICHAEL ANDERS With 2 Figures (Received January II, 1989; accepted March 16, 1989)
Summary Studies on immunohistochemical localization of the epitope b-12 were performed in 15 meningiomas using an indirect immunperoxidase method. 2 of 15 meningiomas stained for b-12. The b-12 antigen represents a further epithelial marker detectable in meningiomas.
1. Introduction Tumour cells with an epithelial and secretory differentiation have been described in ultrastructural and immunohistochemical investigations of meningiomas (ALGUACIL-GARCIA et al. 1986; BUDKA 1982; TERPE et al. 1988). The new anti-epithelial monoclonal antibody b-12 reacts with a glycoprotein of Mr = 350,000 and mucin-like characteristics (STAHLI et al. 1990). It recognizes an intracytoplasmatic, formaldehyde-resistant epitope of a secretory product of normal and malignant epithelial cells (ZENKLUSEN et al. 1988). We report here on the immunohistochemistry of the monoclonal antibody b-12 in tumour cells of meningiomas. To our knowledge, in meningiomas the immunohistochemical positivity for b-12 has not yet been published in the literature.
2. Material and methods Paraffin-embedded blocks of II meningothelial, 2 transitional, I psammomatous, and I angiomatous meningiomas were examined. The 4f.lm paraffin-sections were deparaffinized, pretreated then with 0.1 %-Pronase (Serva, Heidelberg, FRG) and reacted with the monoclonal antibody b-12. As described in detail previously, an indirect immunoperoxidase method (KASPER et aI. 1987), was used. The specifity was controlled by replacement of the monoclonal antibody by phosphate buffered saline (PBS).
3. Results As shown in Figures 1 and 2, there is a focal strong positivity of tumour cells using the monoclonal antibody b-12. 2 of 15 meningiomas (angiomatous and transitional typ) stained for b12! Generally, the staining had a diffuse cytoplasmatic pattern and was rarely membranous. The tumour cells which showed strong positive reaction had hyalin inclusions (pseudopsammoma bodies) .• 6
Acta histochem .• Bd. 87, 2
78
H.-J.
T ERPE
and M.
ANDERS
Fig. I. Immunoperoxidase for b-12 showing staining of some inclusions and cells surrounding them (arrow) . x 280.
Fig. 2. Immunoperoxidase for b-12 showing sharp line of positive reaction , staining the inner luminal cell membranes and the peripheral contour of some inclusions (arrow). x 560.
The immunohistochemical reactivity
79
4. Discussion In meningiomas, intracellular inclusions were first noticed by CUSHING and EISENHARDT (1938). Ultrastructurally they consist of a granular glycoprotein material (KUBOTA et al. 1982). This feature has been interpreted as a secretory product (JANISCH et al. 1988). Immunohistochemical analysis of pseudopsammoma bodies has demonstrated reactivity for both epithelial membrane antigen (EMA) and cytokeratin (MEIS et al. 1986; THEAKER et al. 1986). MEIS et al. (1986) reported on the expression of EMA in 25 (50%) of the meningiomas. This was interpreted as evidence of epithelial-like differentiation. In cells with hyaline inclusions, the expression of the epitope reacting with b-12 is also not surprising because it is a relevant marker for secretory differentiation of normal and malignant epithelial cells (ZENKLUSEN et al. 1988). The b-12 antigen represents a further epithelial marker detectable in meningiomas.
Acknowledgement The authors thank Prof. Dr. F. GUDAT, Department of Pathology, University of Basel, Switzerland, for kindly providing the b-12 antibody.
References ALGUACIL-GARCIA, A., PETTIGREW, N. M., and SIMA, A. A., Secretory meningioma: A distinct subtype of meningioma. Amer. J. Surg. Pathol. 10, 102-111 (1986). BUDKA, H., Hyalin inclusions (pseudopsammoma bodies) in meningiomas: Immunocytochemical demonstration of epithel-like secretion of secretory component and immunoglobulins A and M. Acta Neuropathol. 56, 294- 298 (1982). CUSHING, H., and EISENHARDT, L., Meningiomas. Their Classification, Regional Behavior, Life and Surgical and Results. Springfield/Baltimore: C. C. Thomas 1938. JANISCH, W., SCHREIBER, D., and GUTHERT, H., Neuropathologie - Tumoren des Nervensystems. Jena: VEB Gustav Fischer Verlag 1988. KASPER, M., STOSIEK, P., TYLPT, H., and KARSTEN, U., Histological evaluation of three new monoclonal anticytokeratin antibodies. I. Normal tissues. Europ. J. Cancer Clin. Oncol. 23, 137-147 (1987). KUBOTA, T., HIRANO, A., and YAMAMOTO, S., The fine structure of hyalin inclusions in meningiomas. J. Neuropathol. expo Neurol. 41, 81-86 (1982). MEIS, J. M., ORDONEZ, N. G., and BRUNER, J. M., Meningiomas. Arch. Pathol. Lab. Med. 110,934-937 (1986). STAHL!, c., CARAVATTI, M., AESCHBACHER, M., KOCIBA, c., TAKACS, B., and CARMAN, H., A mucin-like carcinoma associated antigen (MCA) defined by three monoclonal antibodies against different epitopes. Cancer Res. (in press). TERPE, H.-J., KASPER, M., MARTIN, H., und LEHMANN, J., Nachweis von Zytokeratin inZellen der Arachnoidea und in Meningiomen. Zbl. allg. Pathol. pathol. Anat. 134, 259-264 (1988). THEAKER, J. M., GATTER, K. c., ESIRI, M. M., and FLEMMING, K. A., Epithelial membrane antigen andcytokeratin expression by meningiomas: an immunohistochemical study. J. Clin. Pathol. 39,435-439 (1986). ZENKLUSEN, H.-R., STAHL!, c., GUDAT, F., OVERBECK, J. v., ROL!NK, J., and HEITZ, PH. U., The immunohistochemical reactivity of new anti-epithelial monoclonal antibody (Mab b-12) against b{east carcinoma and other normal and neoplastic human tissues. Virchows Arch. A Pathol. Anat. 413, 3- 10 (1988). Authors' address: HANS-JOACHIM TERPE, Institute of Pathology, Department of Medicine, Emst-Moritz-Amdt University, Friedrich-Loeffler-StraBe 23e, Greifswald, GDR - 2200.
Institute of Pathology (Head: Prof. Dr. R. WARZOK), Department of Medicine, Emst-Moritz-Amdt-University, Greifswald, GDR
The immunohistochemical reactivity of an anti-epithelial monoclonal antibody (b-12) in meningiomas By HANS.-JOACHIM TERPE and MICHAEL ANDERS With 2 Figures (Received January II, 1989; accepted March 16, 1989)
Summary Studies on immunohistochemical localization of the epitope b-12 were performed in 15 meningiomas using an indirect immunperoxidase method. 2 of 15 meningiomas stained for b-12. The b-12 antigen represents a further epithelial marker detectable in meningiomas.
1. Introduction Tumour cells with an epithelial and secretory differentiation have been described in ultrastructural and immunohistochemical investigations of meningiomas (ALGUACIL-GARCIA et al. 1986; BUDKA 1982; TERPE et al. 1988). The new anti-epithelial monoclonal antibody b-12 reacts with a glycoprotein of Mr = 350,000 and mucin-like characteristics (STAHLI et al. 1990). It recognizes an intracytoplasmatic, formaldehyde-resistant epitope of a secretory product of normal and malignant epithelial cells (ZENKLUSEN et al. 1988). We report here on the immunohistochemistry of the monoclonal antibody b-12 in tumour cells of meningiomas. To our knowledge, in meningiomas the immunohistochemical positivity for b-12 has not yet been published in the literature.
2. Material and methods Paraffin-embedded blocks of II meningothelial, 2 transitional, I psammomatous, and I angiomatous meningiomas were examined. The 4f.lm paraffin-sections were deparaffinized, pretreated then with 0.1 %-Pronase (Serva, Heidelberg, FRG) and reacted with the monoclonal antibody b-12. As described in detail previously, an indirect immunoperoxidase method (KASPER et aI. 1987), was used. The specifity was controlled by replacement of the monoclonal antibody by phosphate buffered saline (PBS).
3. Results As shown in Figures 1 and 2, there is a focal strong positivity of tumour cells using the monoclonal antibody b-12. 2 of 15 meningiomas (angiomatous and transitional typ) stained for b12! Generally, the staining had a diffuse cytoplasmatic pattern and was rarely membranous. The tumour cells which showed strong positive reaction had hyalin inclusions (pseudopsammoma bodies) .• 6
Acta histochem .• Bd. 87, 2
78
H.-J.
T ERPE
and M.
ANDERS
Fig. I. Immunoperoxidase for b-12 showing staining of some inclusions and cells surrounding them (arrow) . x 280.
Fig. 2. Immunoperoxidase for b-12 showing sharp line of positive reaction , staining the inner luminal cell membranes and the peripheral contour of some inclusions (arrow). x 560.
The immunohistochemical reactivity
79
4. Discussion In meningiomas, intracellular inclusions were first noticed by CUSHING and EISENHARDT (1938). Ultrastructurally they consist of a granular glycoprotein material (KUBOTA et al. 1982). This feature has been interpreted as a secretory product (JANISCH et al. 1988). Immunohistochemical analysis of pseudopsammoma bodies has demonstrated reactivity for both epithelial membrane antigen (EMA) and cytokeratin (MEIS et al. 1986; THEAKER et al. 1986). MEIS et al. (1986) reported on the expression of EMA in 25 (50%) of the meningiomas. This was interpreted as evidence of epithelial-like differentiation. In cells with hyaline inclusions, the expression of the epitope reacting with b-12 is also not surprising because it is a relevant marker for secretory differentiation of normal and malignant epithelial cells (ZENKLUSEN et al. 1988). The b-12 antigen represents a further epithelial marker detectable in meningiomas.
Acknowledgement The authors thank Prof. Dr. F. GUDAT, Department of Pathology, University of Basel, Switzerland, for kindly providing the b-12 antibody.
References ALGUACIL-GARCIA, A., PETTIGREW, N. M., and SIMA, A. A., Secretory meningioma: A distinct subtype of meningioma. Amer. J. Surg. Pathol. 10, 102-111 (1986). BUDKA, H., Hyalin inclusions (pseudopsammoma bodies) in meningiomas: Immunocytochemical demonstration of epithel-like secretion of secretory component and immunoglobulins A and M. Acta Neuropathol. 56, 294- 298 (1982). CUSHING, H., and EISENHARDT, L., Meningiomas. Their Classification, Regional Behavior, Life and Surgical and Results. Springfield/Baltimore: C. C. Thomas 1938. JANISCH, W., SCHREIBER, D., and GUTHERT, H., Neuropathologie - Tumoren des Nervensystems. Jena: VEB Gustav Fischer Verlag 1988. KASPER, M., STOSIEK, P., TYLPT, H., and KARSTEN, U., Histological evaluation of three new monoclonal anticytokeratin antibodies. I. Normal tissues. Europ. J. Cancer Clin. Oncol. 23, 137-147 (1987). KUBOTA, T., HIRANO, A., and YAMAMOTO, S., The fine structure of hyalin inclusions in meningiomas. J. Neuropathol. expo Neurol. 41, 81-86 (1982). MEIS, J. M., ORDONEZ, N. G., and BRUNER, J. M., Meningiomas. Arch. Pathol. Lab. Med. 110,934-937 (1986). STAHL!, c., CARAVATTI, M., AESCHBACHER, M., KOCIBA, c., TAKACS, B., and CARMAN, H., A mucin-like carcinoma associated antigen (MCA) defined by three monoclonal antibodies against different epitopes. Cancer Res. (in press). TERPE, H.-J., KASPER, M., MARTIN, H., und LEHMANN, J., Nachweis von Zytokeratin inZellen der Arachnoidea und in Meningiomen. Zbl. allg. Pathol. pathol. Anat. 134, 259-264 (1988). THEAKER, J. M., GATTER, K. c., ESIRI, M. M., and FLEMMING, K. A., Epithelial membrane antigen andcytokeratin expression by meningiomas: an immunohistochemical study. J. Clin. Pathol. 39,435-439 (1986). ZENKLUSEN, H.-R., STAHL!, c., GUDAT, F., OVERBECK, J. v., ROL!NK, J., and HEITZ, PH. U., The immunohistochemical reactivity of new anti-epithelial monoclonal antibody (Mab b-12) against b{east carcinoma and other normal and neoplastic human tissues. Virchows Arch. A Pathol. Anat. 413, 3- 10 (1988). Authors' address: HANS-JOACHIM TERPE, Institute of Pathology, Department of Medicine, Emst-Moritz-Amdt University, Friedrich-Loeffler-StraBe 23e, Greifswald, GDR - 2200.