The induction of a 2 and a 3 integrin by TPA correlates to the increased collagen gel contraction by fibroblast

112 019

Hcc Chul

Eun, M.D.

Hyung

Chan Pyo, M.D.

and Hong Jik Kim*,

M.D.

Normal human kerar~oncyte culture techmquc IS widely bang used m Authors wanted to compare skm research includmg skin toxicology. qtotoxuty 01’ scvcrill antlblotics and antwptics using human keratmocytes WC used MTT. LDH and trltmted and l’lbroblns& culture models. thymldlne as (1 paramctcr. Kcruinytcs show4 a slightly sensitive cytolouc rcsponsc than flbroblsts. houwcr. the general tendency lo&cd The scnsrt~\ sty of 3 mcth& !vas trlttated th)-midme, MTT and slmlar We thml, the may be helpful for the sclcction of LDH in dccrcas~ng order nc\v topical anclbiotlcs and antwprtcs.

021 THE

INTERACTION BETWEEN BLlFlAN BERM& OUTER RIXl’ SHEATH CELLS 12’ liTR0

T.FUJIE, Departmen l’okushlma,

N UCHIDA,

T SHIKIJI.

PAPILLA

Y URANO and

oI Dermatology, School ot Med~cme, The Tokushrma City. 170 Japan

CELLS AND

(

I-L’JII ~:rsity.

I cdl ISassociated

of HSCand cadherin

with

differential

functions

and ShDhO IMLlr\.MUKh Department of Dermatology. Shogoin. Kawahara cho. Saliyo - ku. lipoto 606.

Iiyoto .lapan

of

In order to study an mferactmn between human dermal papilla cells (DPCs) and outer root sheath cells (ORSCs) in rlfro develo,,ed a new w-culture system with floating collagen membranes. ORSCs were cultured on dishes in the presence of DPCs drown on the membrane. Colony growth of ORSCs was ;igmficantly enhanced an the presence of DPCs at early passage until second passage ). However DPCs ~1 more than sixth passage and skm tibroblasts at any passage inhibited the colony growth. These results suggest thal cultured DPCs at early passage may produce a factor(s) which enhances the growth ot ORSCs. but rhr cells gradually lose this actiwy along wth the passage.

we

induced scattering IIIIICIUIJ~IO~ of ~ntcgrln lil\liO Lnl\

S AR&SE University

022 I~:(;1

DISC I squamous carcinoma cells show anlpllfled EGr; receptortF:GFRI RCIIC and overexpression of the receptor. thus providinga suitable model for studying the biological role of EGF ‘liCFfI system m turno~ cell biology. EGF caused dispersion of HSCwhich had formed Such ccl1 scattering ~85 compact colonies in monolayer culture. of mtegrln~mrdlawd aswclated with approximately 50 OO reduction cvll spl-cndlng to fibroncctin. fibrinogen and lamlnln. and wth -1 Cold iwrcilsc of cell spreading and ccl1 migration DVC~ type also caused transienl macttvotion of cadhrr~n E~medratcd c&l <.<.I1 ,~dhes~on. Our observation suggests that EGF EGFR systcn, can ommotc tumor cell detachment and mlgrali,ln from the primar’) Ion14 by modulating both integrinand c:ldherIn functions.

I cells

I collagen.

lXT1;

024 lNTRACEI.I.ULAR

SUPPRESIVE

FACTOR

OF ICAM- EXPRESSION

HIROSHI FUJISAWA, TAKASHI NOMURA, FUJIU OTSUKA, Department of Dermatology, Institute of Clinical Medicine. University of Tsukuba, Tennodai, Tsukuba city, lbaraki prefectures, Japan In this study, intracellular factor which suppresses thr expression of iniercellular adhesion molecule (IChY)-1 was investigated by Nothern-blots analysis using cell lines derived from human squamous cell carcinoma. Interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha induce LCAM-1 expression in these cell lines. Ca-treatment with cycloheximidr or purunycin, both of which inhibit protein synthesis by different mechanisms remarkably increase IFNgamma or TNF-alpha-induced ICAY- mKNA within 4 hours. In contrast the actin mRNA level was almost unaffected by the same treatments. Thus it is suggested chat there is an intracellular prntrin which suppresses TFN-gamma of TNF-alpha-induced IC~L'-1 expression.