The influence of EDTA on adenylate cyclase activity in membranes from rat (BBA 12147)

267

Erratum

B i o c h i m i c a et B i o p h y s i c a A c t a 931 (1987) 2 3 4 - 2 4 1 T h e i n f l u e n c e o f E D T A o n a d e n y l a t e c y c l a s e a c t i v i t y in m e m b r a n e s f r o m r a t . . . ( B B A 12147) S t e p h e n A. M o r r i s a n d J o h n P. B e l e z i k i a n ( U . S . A . )

p. 239, T a b l e II s h o u l d a p p e a r t h u s :

TABLE II QUANTIFICATION OF CHOLERA TOXIN (CT)-DEPENDENT AND -INDEPENDENT ADP-RIBOSYLATION IN MOUSE AND RAT HEART MEMBRANES Mouse and rat heart membranes were analyzed from Laemmli gels of ADP-ribosylation reactions. The calculated magnitude of incorporation of label in the 42 kDa band in the absence of cholera toxin for membranes prepared in the presence of EDTA was assigned a value of 100%. All other readings for membranes prepared in the presence or absence of EDTA were normalized to this value, and expressed as percent. Analysis shown here is one of four separate pairs of gels, all qualitatively similar. Concentrations of GTP and Gpp[NH]p were 100 btM each. Addition to ADP-ribosylation

Preparation of membranes

None

+ EDTA

% Incorporation of [32p]ADP ribose mouse

rat

100

100

CT, GTP, NAD (5 BM), CT, GTP, NAD (200/~M)

207 175

225 95

CT, Opp[NH]p, NAD (5/tM) CT, Opp[NH]p, NAD (200/~M)

147 152

142 148

40

60

CT, GTP, NAD (5/LM) CT, GTP, NAD (200 p,M)

152 28

139 68

CT, Gpp[NH]p, NAD (5 #M) CT, Gpp[NH]p, NAD (200 btM)

148 68

152 107

None

- EDTA