The influence of some pesticides, artificial manures and detergents on the chromosome aberrations in fish

234 females of the C3H strain used in the experiment were treated with TEM for 5 successive days. TEM was administered by gavage in one group before and in the other after mating in doses of 0.2 and 1.0 mg/kg body weight. Mating was performed in the ratio 10 treated females : 3 normal males for 7 days. 17 days after their initial exposure ~o a male, all females were killed and their uteri examined for the total number of implants and the percentage of fetal mortal. ity and resorptions per pregnant female. Our results showed that TEM reduces the fertility in both groups and in both doses. The rate of fertility was from 16 to 24%. The higher percentage of fertility was when TEM had been administered before mating and the higher percentage of resorptions was in the case when TEM was given after mating.

130 A. Sofrad~ija and T. VukoviS, Institute of Biology, Saraj evo (Yugoslavia) The influence o f some pesticides, artificial manures and detergents on the chromosome aberrations in fish Preliminary data on the influence of pesticides, detergents and artificial manures on the chromosome aberrations in fish have been presented. The effect of different concentrations of pesticides Undan and Simazin on the mitotic activity and chromosome aberrations in species Alburnus alburnus was analysed. Relatively weak concentrations of these compounds retard mitotic activity and provoke chromosome aberrations in the kidney cells of this fish. It is also established t h a t different concentrations of " A V A " detergent and combined artificial manures retard mitotic activity and cause the changes in chromosome complements in species Alburnus alburnus and Alburnoides bipunctatus.

131 R. Strobel, A. Basler, I. Theiss and G. RShrborn, Institut ffir Humangenetik, Dfisseldorf (Federal Republic of Germany) Carcinogenic and mutagenic effects induced by busulfan Busulfan (Myleran ®), an alkylating agent, is clinically used against myetoid leukemia. Carcinogenicity of this drug could be demonstrated, using the cell transformation assay according to Styles (1977). Mutagenicity could be demonstrated to mammals in vivo (Basler, 1979} and f in the Salmonella~microsome test. In bone marrow, a dose-dependent linear increase in the frequency of chromosome aberrations was observed. An intermediate c o m p o u n d of DNA replication seems to be necessary to translate busulfan-induced lesions in oocytes into chromosome aberrations. In embryonic liver cells of transplacentally exposed mice, chromosome aberrations were induced. The experimental data indicate follow-up studies of diaplacentally exposed