- Email: [email protected]
THE MEDIATING EFFECTS OF STRESS ON DYNAMIC CHANGE IN PHYSICAL ACTIVITY AND COGNITIVE DECLINE IN AGING
P1178
Poster Presentations: Wednesday, July 27, 2016
71.7% and 41.7%, respectively. MNA results showed that only 11.7% of the old man was in good nutrition, up to 68.3% was in potential risk of malnutrition and 20.0% was malnutrition. Staple foods, milk and products, vegetables and edible oil were 233.3g/ day, 46.5 g/day, 165.0 g/day and 6.4 g/day, respectively. Results of ADL survey showed that disabled accounted for 28.3%, and in which 58.9% of light disabled, 23.6% for moderate and 17.6% for severe disabled. Subjects of BMI<18.5kg/m2accounted for 36.6%, anemia, low-protein and low-albumin accounted for 56.7%,16.7% and 46.7%, respectively. From the MoCA Scale test, the declined cognitive abilities accounted for 48.1% totally. The correlation coefficient between cognitive score and staple foods, milk and products, beans, vegetables was 0.97, 0.90, 0.94 and 0.97 respectively. Conclusions: The nutrition and cognition ability in nursing homes was severe and more attention should be paid for improvement of nutrition prevention among older adults lived in nursing home.
P4-367
ONLINE REGISTRY FOR THE PREVENTION OF DEMENTIA IN JAPAN
Hiroshi Matsuda1, Hidehiro Mizusawa1, Norihide Maikusa1, Etsuko Imabayashi1, Masayo Ogawa1, Kenji Toba2, Hiroyuki Shimada2, Keisuke Suzuki2, 1National Center of Neurology and Psychiatry, Kodaira, Japan; 2National Center for Geriatrics and Gerontology, Obu, Japan. Contact e-mail: [email protected] Background: With the rapid aging of the global population, cognitive decline and dementia pose large-scale healthcare and socioeconomic burdens. Strategies to address these burdens are urgently needed, particularly for dementia due to Alzheimer’s disease (AD). Such strategies include the development of disease modifying therapies (DMT) for AD but must also incorporate a preventive approach to reducing risk factors. Over the past 10 years, AD DMT clinical trials have been largely unsuccessful, partly due to difficulty in recruiting early stage patients for enrollment. In addition, successful AD prevention trials will require a better understanding of the complex relationships between cognitive and functional abilities, risk factors, and life style choices. Establishing a large-scale registry for healthy and at risk individuals will play a key role in efficiently addressing these issues. Methods: As a part of National Project called “Orange Plan,” we have developed a nation-wide, online registry system called IROOP (Integrated Registry of Orange Plan) for healthy or at risk individuals over 40 years old in Japan (https://iroop.jp). Every 6 months, registered participants answer an online questionnaire including demographics, mood, quality of life, sleep, and so on, and take a brief neuropsychological assessment (MCI Screen [MCIS]) over the phone. The MCIS includes the well-validated 10-word recall protocol used in the CERAD and ADAS-Cog batteries. Results: Full assessment results are made available electronically to participants. The gathered data will be used to investigate the risk factors associated with cognitive changes in pre-symptomatic or -clinical stages of cognitive impairment (CI), and identify preventive strategies for delaying the onset of CI. In addition, the IROOP registry will be used to efficiently enroll subjects for AD DMT clinical trials and AD prevention trials. Conclusions: The recruitment of cognitively healthy or at risk individuals is a key factor for
improving the success rate of AD trials. By incorporating a well-established, online questionnaire accompanied by regular cognitive assessment, we will enable a more effective approach to ongoing research and demonstrate a roadmap for progress in this field. The IROOP registry system is expected to demonstrate a viable approach to accelerating global progress toward dementia delay and prevention.
P4-368
THE MEDIATING EFFECTS OF STRESS ON DYNAMIC CHANGE IN PHYSICAL ACTIVITY AND COGNITIVE DECLINE IN AGING
Rebecca Vendittelli1, Andrea Piccinin1, Andrey Koval2, Raquel Beth Graham1, 1University of Victoria, Victoria, BC, Canada; 2 University of Victoria, Victoria, BC, Canada. Contact e-mail: [email protected] Background: The moderating effect of stress on the relationship between the dynamic change in physical activity and cognitive outcomes with ageing will be explored. It is expected that chronic stress will moderate the effects of physical activity, so that cognitive decline will be more probable in those who are less active when compared to those who are more active and experience similar levels of chronic stress. Methods: Two longitudinal data sets from the Integrative Analysis of Longitudinal Studies of Aging (IALSA) network will be analysed in a coordinated manner in order to compare and contrast findings, comment on generalizability and contribute to the reproducibility of scientific knowledge. Specifically the Oregon Brain Aging Study (OBAS) and Memory and Aging Project (MAP) data will be analysed. Results: To be determined. Conclusions: To be determined.
P4-369
FOLIC ACID MODULATE PRESENILIN 1 INHIBITS AMYLOID b-PEPTIDE PRODUCTION IN N2A-APP CELLS
Wen Li, Huan Liu, Xumei Zhang, Guowei Huang, Tianjin Medical University, Tianjin, China. Contact e-mail: [email protected] Background: Alzheimer’s disease (AD) is the most prevalent type of dementia. Folic acid supplementation is a potential therapeutic intervention. We hypothesized that folic acid supplementation may alter amyloid b-peptide (Ab) production in AD because the expression of amyloid precursor protein (APP) and presenilin (PS) genes can be modulated. Methods: Mouse Neuro-2a cells expressing human APP695 were incubated with folic acid (2.8 - 40 mmol/L) for 96 h. And then immunofluorescence staining, RTPCR assay, western blot assay, and HPLC were used to detect methylation potential and Ab, APP, and PS1 expression in N2aAPP cells. Results: The results showed that folic acid stimulated methylation potential. Furthermore, folic acid decreased APP and PS1 expression as well as Ab protein production. There was statistically significant dose-response relationship between Ab production and folic acid (R¼-0.991, P¼0.000), and PS1 protein expression, SAM:SAH also had statistically significant doseresponse relationship with folic acid, the pearson correlation coefficient were -0.848 (P¼0.000) and 0.943 (P¼0.000) respectively. Conclusions: The results indicate that folic acid induces methylation potential that could decrease PS1 expression, thereby attenuating Ab production.
Poster Presentations: Wednesday, July 27, 2016
71.7% and 41.7%, respectively. MNA results showed that only 11.7% of the old man was in good nutrition, up to 68.3% was in potential risk of malnutrition and 20.0% was malnutrition. Staple foods, milk and products, vegetables and edible oil were 233.3g/ day, 46.5 g/day, 165.0 g/day and 6.4 g/day, respectively. Results of ADL survey showed that disabled accounted for 28.3%, and in which 58.9% of light disabled, 23.6% for moderate and 17.6% for severe disabled. Subjects of BMI<18.5kg/m2accounted for 36.6%, anemia, low-protein and low-albumin accounted for 56.7%,16.7% and 46.7%, respectively. From the MoCA Scale test, the declined cognitive abilities accounted for 48.1% totally. The correlation coefficient between cognitive score and staple foods, milk and products, beans, vegetables was 0.97, 0.90, 0.94 and 0.97 respectively. Conclusions: The nutrition and cognition ability in nursing homes was severe and more attention should be paid for improvement of nutrition prevention among older adults lived in nursing home.
P4-367
ONLINE REGISTRY FOR THE PREVENTION OF DEMENTIA IN JAPAN
Hiroshi Matsuda1, Hidehiro Mizusawa1, Norihide Maikusa1, Etsuko Imabayashi1, Masayo Ogawa1, Kenji Toba2, Hiroyuki Shimada2, Keisuke Suzuki2, 1National Center of Neurology and Psychiatry, Kodaira, Japan; 2National Center for Geriatrics and Gerontology, Obu, Japan. Contact e-mail: [email protected] Background: With the rapid aging of the global population, cognitive decline and dementia pose large-scale healthcare and socioeconomic burdens. Strategies to address these burdens are urgently needed, particularly for dementia due to Alzheimer’s disease (AD). Such strategies include the development of disease modifying therapies (DMT) for AD but must also incorporate a preventive approach to reducing risk factors. Over the past 10 years, AD DMT clinical trials have been largely unsuccessful, partly due to difficulty in recruiting early stage patients for enrollment. In addition, successful AD prevention trials will require a better understanding of the complex relationships between cognitive and functional abilities, risk factors, and life style choices. Establishing a large-scale registry for healthy and at risk individuals will play a key role in efficiently addressing these issues. Methods: As a part of National Project called “Orange Plan,” we have developed a nation-wide, online registry system called IROOP (Integrated Registry of Orange Plan) for healthy or at risk individuals over 40 years old in Japan (https://iroop.jp). Every 6 months, registered participants answer an online questionnaire including demographics, mood, quality of life, sleep, and so on, and take a brief neuropsychological assessment (MCI Screen [MCIS]) over the phone. The MCIS includes the well-validated 10-word recall protocol used in the CERAD and ADAS-Cog batteries. Results: Full assessment results are made available electronically to participants. The gathered data will be used to investigate the risk factors associated with cognitive changes in pre-symptomatic or -clinical stages of cognitive impairment (CI), and identify preventive strategies for delaying the onset of CI. In addition, the IROOP registry will be used to efficiently enroll subjects for AD DMT clinical trials and AD prevention trials. Conclusions: The recruitment of cognitively healthy or at risk individuals is a key factor for
improving the success rate of AD trials. By incorporating a well-established, online questionnaire accompanied by regular cognitive assessment, we will enable a more effective approach to ongoing research and demonstrate a roadmap for progress in this field. The IROOP registry system is expected to demonstrate a viable approach to accelerating global progress toward dementia delay and prevention.
P4-368
THE MEDIATING EFFECTS OF STRESS ON DYNAMIC CHANGE IN PHYSICAL ACTIVITY AND COGNITIVE DECLINE IN AGING
Rebecca Vendittelli1, Andrea Piccinin1, Andrey Koval2, Raquel Beth Graham1, 1University of Victoria, Victoria, BC, Canada; 2 University of Victoria, Victoria, BC, Canada. Contact e-mail: [email protected] Background: The moderating effect of stress on the relationship between the dynamic change in physical activity and cognitive outcomes with ageing will be explored. It is expected that chronic stress will moderate the effects of physical activity, so that cognitive decline will be more probable in those who are less active when compared to those who are more active and experience similar levels of chronic stress. Methods: Two longitudinal data sets from the Integrative Analysis of Longitudinal Studies of Aging (IALSA) network will be analysed in a coordinated manner in order to compare and contrast findings, comment on generalizability and contribute to the reproducibility of scientific knowledge. Specifically the Oregon Brain Aging Study (OBAS) and Memory and Aging Project (MAP) data will be analysed. Results: To be determined. Conclusions: To be determined.
P4-369
FOLIC ACID MODULATE PRESENILIN 1 INHIBITS AMYLOID b-PEPTIDE PRODUCTION IN N2A-APP CELLS
Wen Li, Huan Liu, Xumei Zhang, Guowei Huang, Tianjin Medical University, Tianjin, China. Contact e-mail: [email protected] Background: Alzheimer’s disease (AD) is the most prevalent type of dementia. Folic acid supplementation is a potential therapeutic intervention. We hypothesized that folic acid supplementation may alter amyloid b-peptide (Ab) production in AD because the expression of amyloid precursor protein (APP) and presenilin (PS) genes can be modulated. Methods: Mouse Neuro-2a cells expressing human APP695 were incubated with folic acid (2.8 - 40 mmol/L) for 96 h. And then immunofluorescence staining, RTPCR assay, western blot assay, and HPLC were used to detect methylation potential and Ab, APP, and PS1 expression in N2aAPP cells. Results: The results showed that folic acid stimulated methylation potential. Furthermore, folic acid decreased APP and PS1 expression as well as Ab protein production. There was statistically significant dose-response relationship between Ab production and folic acid (R¼-0.991, P¼0.000), and PS1 protein expression, SAM:SAH also had statistically significant doseresponse relationship with folic acid, the pearson correlation coefficient were -0.848 (P¼0.000) and 0.943 (P¼0.000) respectively. Conclusions: The results indicate that folic acid induces methylation potential that could decrease PS1 expression, thereby attenuating Ab production.