The most frequent aneuploidies in human embryo are similar to those observed in the early pregnancy loss

CONCLUSION: This data suggest, although rates of blastocyst formation are reduced with advanced reproductive age, acceptable rates of blastocyst formation can be observed for all patients. A decrease in blastulation can be observed for all ICSI cycles versus traditional insemination. Rates of blastocyst formation were observed to increase over time. P-626 Wednesday, October 22, 2014

Supported by: Red Rock Fertility Center.

THE MOST FREQUENT ANEUPLOIDIES IN HUMAN EMBRYO ARE SIMILAR TO THOSE OBSERVED IN THE EARLY PREGNANCY LOSS. E. Littman, V. Phan, D. Harris, M. Severino, A. La. Red Rock Fertility Center, Las Vegas, NV. OBJECTIVE: To investigate whether the meiotic errors that are seen in cleavage stage embryo are the ones observed in the first trimester of pregnancy. DESIGN: Retrospective Study in an In Vitro Fertilization Laboratory. MATERIALS AND METHODS: Embryos from 156 patients (average age 34.7 years 5.5) were investigated. A-CGH was used to investigate 23 pairs of chromosomes of a single blastomere biopsied on day 3 of embryo development. RESULTS: A total of 877 day 3 embryos were analyzed, of which 474 were euploid and 403 were aneuploid. Aneuploidy can happen in any chromosomes. In the following order, the most frequently involed in aneuploidy were chromosomes 22, 19, 16, 15, 21, XY, 9, 13, 18, 1, 20, 14, 11, 4, 12, 2, 6, 3, 7, 17, 8, 5, 10. CONCLUSION: Aneuploidy is strongly affected by maternal age. Most embryos cannot survive with a missing or extra chromosome and are spontaneously aborted. This study partially corroborated with previous publications reporting chromosomal aberrations in first trimester abortion. Our results show that the most frequent aneuploidy involves the chromosome 22, 19, 16, 15, 21, XY, 9, 13,18. As observed in abortus’ karyotypes; trisomy 22, 16 and 15 are common found in the first trimester miscarriages and account for 3.8%; 4.5%; 5.2% of SABs respectively. Viable trisomies have been observed for chromosomes 13,18 and 21. Monosomy X (Turner syndrome) is

Frequency of Chromosome Aneuploidy

Chromosome #

Aneuploid

Euploid

RR

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 XY

25(2.9%) 17(1.9%) 13(1.5%) 18(2.1%) 10(1.1%) 15(1.7%) 12(1.4%) 11(1.3%) 30(3.4%) 10(1.1%) 18(2.1%) 17(1.9%) 29(3.3%) 19(2.2%) 49(5.6%) 54(6.2%) 12(1.4%) 28(3.2%) 56(6.4%) 24(2.7%) 44(5.0%) 74(8.4%) 35(4%)

852(97.1%) 860(98.1%) 864(98.5%) 859(97.9%) 867(98.9%) 862(98.3%) 865(98.6%) 866(98.7%) 847(96.6%) 867(98.9%) 859(97.9%) 860(98.1%) 848(96.7%) 858(97.8%) 828(94.4%) 823(93.8%) 865(98.6%) 849(96.8%) 821(93.6%) 853(97.3%) 833(95%) 803(91.6%) 842(96%)

2.64 1.73 1.36 1.91 1.0 1.55 1.27 1.18 3.09 1.0 1.91 1.73 3.0 2.0 5.09 5.64 1.27 2.91 5.82 2.45 4.55 7.64 3.64

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ASRM Abstracts

frequently observed and accounts for 4.3% of SABs. Our results demonstrate that a-CGH-for detection of aneuploidies in IVF embryos is an accurate diagnosis strategy. In addition, the transfer of normal embryos prevent miscarrage and the occurrence of pregnancies resluting in the birth of children with multiple healthy problems due to aneuploidy.

P-627 Wednesday, October 22, 2014 MEIOTIC SPINDLE LOCATION OF MATURE OOCYTES ORIGINATED FROM STIMULATED CYCLES IS A PREDICTOR FOR BLASTOCYST FORMATION. A. Mahfoudh, E. Garcia-Cerrudo, J. H. Moon, S. Henderson, H. Holzer, W.-Y. Son. Obstetrics and Gynecology, MUHC Reproductive Center. McGill University, Montreal, QC, Canada. OBJECTIVE: The purposes of this study are: 1) to evaluate the relationship between the presence of spindle in metaphase II (MII) oocytes and their potential to develop blastocyst, and 2) to analyze the clinical pregnancy rate depending on the origin of the embryo transferred derived from the different spindle angle. DESIGN: Retrospective study. MATERIALS AND METHODS: This study analyzed 58 patients who underwent IVF from January-December 2013. A total of 830 eggs were collected of which 648 were MII on retrieval day. Oocytes were screened using PolscopeTM to visualize the meiotic spindle to avoid damaging the spindle during ICSI and characterized in terms of visible or non-visible spindle and its position in relation to the first polar body (PB). From the 648 MII oocytes, 581 (89%) had visible spindles and were separated into 3 groups: (group 1) 0 -29 ; (group 2) 30 -89 ; (group 3) R90 and those with no visible spindle into group 4. Blastocyst developmental potential and clinical pregnancy rate were analyzed depending on the origin of the single embryo transferred (SET). Data was analyzed using SPSS. Chi-square test and logistic regression were performed. P-values <0.05 were considered statistically significant. RESULTS: There was no significant difference in fertilization and cleavage rates among the groups. Blastocyst formation rate in group 1 (0 -29 ) was significantly higher than the other groups (P<0.001). Better clinical pregnancy rate was observed in the cycles where embryos transferred had been derived from group 1 oocytes (26/58, 44.9%) than in those where embryos were derived from other groups (group 2: 5/58, 8.6%; group 3: 3/58, 5.1%; group 4: 6/58, 10.3%), however this difference did not reach statistical significance.

IVF results based on spindle angle.

Spindle Angle

Matured (%)

Fertilized (%)

Cleavage (%)

Blastocyst (%)

0-29 30-89 R90 No visible Total P-Value

292 (45.1) 219 (33.8) 64 (9.9) 73 (11.2) 648 (78.1) 0.48

202 (43.1) 167 (35.6) 51 (10.9) 49 (10.4) 469 (73.5) 0.09

204 (43.3) 167 (35.5) 51 (10.8) 49 (10.4) 471 (56.7) 0.128

111 (47.4) a 87 (37.2) b 25 (10.7) b 11 (4.7) b 234 (28.2) <0.001

a vs. b P<0.01 CONCLUSION: Meiotic spindle of oocytes located between 0 -29 is a predictor for blastocyst developmental competence.

Vol. 102, No. 3, Supplement, September 2014