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The preparation of fluorescent nanoparticles based on poly(p-phenylene vinylene) and their application in developing latent fingerprint
Abstracts / Journal of Controlled Release 259 (2017) e5–e195
universal and effective method for the intracellular delivery of proteins and peptides. Keywords: protein delivery, peptide delivery, dendrimer, polymer, rational design Acknowledgements We thank financial support from the National Natural Science Foundation of China (No. 21322405 and No. 21474030) and the Shanghai Municipal Science and Technology Commission (148014518) on this work. References [1] J.B. Rothbard, T.C. Jessop, R.S. Lewis, B.A. Murray, P.A. Wender. Role of membrane potential and hydrogen bonding in the mechanism of translocation of guanidiniumrich peptides into cells, J. Am. Chem. Soc. 126 (2004) 9506-9507. [2] A.C. Fonseca, M.A. Frías, A.M. Bouchet, S. Jarmelo, P.N. Simões, R. Fausto, M.H. Gil, F. Lairion, E.A. Disalvo, Role of guanidyl moiety in the insertion of arginine and Nαbenzoyl-L-argininate ethyl ester chloride in lipid membranes, J. Phys. Chem. B. 114 (2010) 5946-5952.
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characterized by Dynamic Light Scattering (DLS) and Transmission Electron Microscope (TEM). The developing process was accomplished simply by immersing the object with LFP into NP suspension. The experimental results suggest that the PPV NPs are very effective in developing LFP on impermeable substrates, such as glass sheet, aluminum foil and so forth, especially on the adhesive surface of tape. Keywords: poly(p-phenylene vinylene), nanoparticles, fluorescence, latent fingerprint Acknowledgements This work was supported by the National Natural Science Foundation of China (No. 21374071). References [1] X. Ran, Z.Z. Wang, Z.J. Zhang, F. Pu, J.S. Ren, and X.G. Qu, Nucleic-acid-programmed Ag-nanoclusters as a generic platform for visualization of latent fingerprints and exogenous substances, Chem. Commun. 52 (2016) 557-560. [2] Y. Chen, H. Chen, H. Zhang, and L.J. Fan, Color tuning of core-shell fluorescent microspheres by controlling the conjugation of poly (p-phenylenevinylene) backbone, ACS Appl. Mater. Interfaces 7 (2015) 26709-26715.
doi:10.1016/j.jconrel.2017.03.109 doi:10.1016/j.jconrel.2017.03.110
The preparation of fluorescent nanoparticles based on poly(p-phenylene vinylene) and their application in developing latent fingerprint Hong Chen, Yun Chen, Li-Juan Fan⁎ Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China ⁎Corresponding author. E-mail addresses: [email protected] (H. Chen), [email protected] (L.-J. Fan) Fluorescent conjugated polymer nanoparticles have wide applications in display device, bioimaging and sensing due to their superior fluorescence properties. Latent fingerprints (LFP) have been particularly important physical evidences in crime scene, while they usually cannot be seen with naked eyes unless being treated by special methods [1]. Developing a new strategy for fingerprint development based on fluorescent nanoparticles will be of great practical significance. In this study, we successfully prepared nanoparticles based on poly(p-phenylene vinylene) (PPV) with a simple and effective route; and these nanoparticles in suspension were used to develop the latent fingerprint (LFP) (Fig. 1). The water soluble PPV precursor (pre-PPV) was synthesized via Wessling method [2], and the nanoparticles (NPs) were obtained by adding sodium dodecyl sulfonate (SDS) as surfactant during the thermal elimination process. The nanoparticles were
Fig. 1. (a) The schematic of preparing PPV nanoparticles and developing latent fingerprint (LFP); (b) the chemical structures and reaction employed in this study.
Charge-convertible poly(β-L-malic acid)-based micelles for effective anticancer drug delivery Qing Zhou, Youbei Qiao, Songyan Guo, Li Fan, Tiehong Yang, Hong Wu⁎ Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China ⁎Corresponding author. E-mail addresses: [email protected] (Q. Zhou), [email protected] (H. Wu) Poly(β-L-malic acid) (PMLA), a natural aliphatic polyester, has been proven to be a promising carrier for anti-cancer drugs. In spite of excellent biocompatibility and degradability, the application of PMLA for cancer therapy is hindered by its low cellular uptake efficiency. The strong negative charge of PMLA impedes the process of endocytosis due to strong electrostatic repulsion with negatively charged cancer cell membrane. In this study, we report the design and development of pH-sensitive charge-convertible PMLA-based micelles for effective anti-tumor drug delivery, enhanced cellular uptake and triggered drug release inside the tumor cell. PMLA was first modified with polyethylenimine (PEI) to allow the polymer to bear positive charge. Covalently conjugation of doxorubicin (DOX) with PMLA afforded amphiphilic co-polymers that readily formed micelles. Furthermore, cell penetrating peptide was used to promote
Fig. 1. Distribution and tumor accumulation of micelles in tumor-bearing mice.
universal and effective method for the intracellular delivery of proteins and peptides. Keywords: protein delivery, peptide delivery, dendrimer, polymer, rational design Acknowledgements We thank financial support from the National Natural Science Foundation of China (No. 21322405 and No. 21474030) and the Shanghai Municipal Science and Technology Commission (148014518) on this work. References [1] J.B. Rothbard, T.C. Jessop, R.S. Lewis, B.A. Murray, P.A. Wender. Role of membrane potential and hydrogen bonding in the mechanism of translocation of guanidiniumrich peptides into cells, J. Am. Chem. Soc. 126 (2004) 9506-9507. [2] A.C. Fonseca, M.A. Frías, A.M. Bouchet, S. Jarmelo, P.N. Simões, R. Fausto, M.H. Gil, F. Lairion, E.A. Disalvo, Role of guanidyl moiety in the insertion of arginine and Nαbenzoyl-L-argininate ethyl ester chloride in lipid membranes, J. Phys. Chem. B. 114 (2010) 5946-5952.
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characterized by Dynamic Light Scattering (DLS) and Transmission Electron Microscope (TEM). The developing process was accomplished simply by immersing the object with LFP into NP suspension. The experimental results suggest that the PPV NPs are very effective in developing LFP on impermeable substrates, such as glass sheet, aluminum foil and so forth, especially on the adhesive surface of tape. Keywords: poly(p-phenylene vinylene), nanoparticles, fluorescence, latent fingerprint Acknowledgements This work was supported by the National Natural Science Foundation of China (No. 21374071). References [1] X. Ran, Z.Z. Wang, Z.J. Zhang, F. Pu, J.S. Ren, and X.G. Qu, Nucleic-acid-programmed Ag-nanoclusters as a generic platform for visualization of latent fingerprints and exogenous substances, Chem. Commun. 52 (2016) 557-560. [2] Y. Chen, H. Chen, H. Zhang, and L.J. Fan, Color tuning of core-shell fluorescent microspheres by controlling the conjugation of poly (p-phenylenevinylene) backbone, ACS Appl. Mater. Interfaces 7 (2015) 26709-26715.
doi:10.1016/j.jconrel.2017.03.109 doi:10.1016/j.jconrel.2017.03.110
The preparation of fluorescent nanoparticles based on poly(p-phenylene vinylene) and their application in developing latent fingerprint Hong Chen, Yun Chen, Li-Juan Fan⁎ Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China ⁎Corresponding author. E-mail addresses: [email protected] (H. Chen), [email protected] (L.-J. Fan) Fluorescent conjugated polymer nanoparticles have wide applications in display device, bioimaging and sensing due to their superior fluorescence properties. Latent fingerprints (LFP) have been particularly important physical evidences in crime scene, while they usually cannot be seen with naked eyes unless being treated by special methods [1]. Developing a new strategy for fingerprint development based on fluorescent nanoparticles will be of great practical significance. In this study, we successfully prepared nanoparticles based on poly(p-phenylene vinylene) (PPV) with a simple and effective route; and these nanoparticles in suspension were used to develop the latent fingerprint (LFP) (Fig. 1). The water soluble PPV precursor (pre-PPV) was synthesized via Wessling method [2], and the nanoparticles (NPs) were obtained by adding sodium dodecyl sulfonate (SDS) as surfactant during the thermal elimination process. The nanoparticles were
Fig. 1. (a) The schematic of preparing PPV nanoparticles and developing latent fingerprint (LFP); (b) the chemical structures and reaction employed in this study.
Charge-convertible poly(β-L-malic acid)-based micelles for effective anticancer drug delivery Qing Zhou, Youbei Qiao, Songyan Guo, Li Fan, Tiehong Yang, Hong Wu⁎ Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China ⁎Corresponding author. E-mail addresses: [email protected] (Q. Zhou), [email protected] (H. Wu) Poly(β-L-malic acid) (PMLA), a natural aliphatic polyester, has been proven to be a promising carrier for anti-cancer drugs. In spite of excellent biocompatibility and degradability, the application of PMLA for cancer therapy is hindered by its low cellular uptake efficiency. The strong negative charge of PMLA impedes the process of endocytosis due to strong electrostatic repulsion with negatively charged cancer cell membrane. In this study, we report the design and development of pH-sensitive charge-convertible PMLA-based micelles for effective anti-tumor drug delivery, enhanced cellular uptake and triggered drug release inside the tumor cell. PMLA was first modified with polyethylenimine (PEI) to allow the polymer to bear positive charge. Covalently conjugation of doxorubicin (DOX) with PMLA afforded amphiphilic co-polymers that readily formed micelles. Furthermore, cell penetrating peptide was used to promote
Fig. 1. Distribution and tumor accumulation of micelles in tumor-bearing mice.