- Email: [email protected]
The protective antioxidant effect of Lemon balm extract against UVB-induce damage in a skin cell model
D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107
Keywords: Antioxidant; ROS; Lemon balm; Polyphenol; Keratinocyte
Solar radiation exposure is the main cause of a variety of cutaneous disorders, including photoageing and skin cancers. Ultraviolet radiation (UV), especially UVB, causes DNA damage, pyrimidine dimmers, 8-hydroxy-2´-deoxyguanosine, p53 induction, protein oxidation and generation of reactive oxygen species (ROS) formation. Polyphenol species of Mediterranean plants have shown photoprotective effects. This study evaluated the protective action of lemon balm extract (LB) against UVB-induced damage in human keratinocytes (HaCaT cells) and correlates both activities. Antioxidant activity has been measured using up to four in vitro different assays (Folin-Ciocalteu assay, trolox equivalent antioxidant capacity, ferric reducing-antioxidant power and oxygen radical absorbance capacity assays). On the other hand, cell viability of HaCaT exposed to UVB was determined by MTT assay. Finally antioxidant activity by ROS-sensitive dichlorofluorescein diacetate was measured confirming the correlation between antioxidant and protective activities. In conclusion, our results suggest that LB extract present a potential photoprotective effect in human skin cell model against UVB-induced damage mediated by its antioxidant capacity, however, further research should be developed to elucidate molecular mechanisms. E-mail address: [email protected] (V. Ruiz-Torres) Acknowledgements
AGL2015-67995-C3-1-R from the Spanish Ministry of Science and Innovation, PROMETEO/2016/006 from GV, Instituto de Salud Carlos III and GV for VALi+D fellowships (ACIF/2015/158). http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.318
P-234
Glut 1 overexpression prevents glucose deprivationinduced prostate cancer cell death by increasing pentose phosphate pathway and glutathione David Hevia 1,2, Pedro Gonzalez-Menendez 1,2, Vanesa Cepas 1,2, Rosa M Sainz 1,2 1
Department of Morphology and Cell Biology, University of Oviedo University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Spain
2
Keywords: prostate cancer; Glut 1; apoptosis; oxidative stress; glutathione
Although glucose metabolism was override in prostate cancer, it is recognized that there are metabolic differences between androgen-sensitive and castration-resistant phenotypes that might be responsible of progression or treatment success. Among the targets of glucose metabolism, glucose uptake is on the top of the cascade. The increase of glucose uptake by GLUTs overexpression in oncogenesis or the androgenic regulation of some GLUT transporters in other tissues is demonstrated. The prostate, at the beginning of carcinogenesis, depends on OXPHOS but then, at aggressive stages, tumors become again glycolytic like other cancers. Therefore, their resistance to glucose deprivation is different at the beginning or at later stages. In this work, we demonstrated by using androgen sensitive and insensitive cells that facilitative
S99
GLUT1 transporter overexpression protect prostate cancer cells from apoptosis caused by glucose removal. Glucose removal caused an increment in oxidative stress that stimulated androgen receptor activity and GLUT1 overexpression. This protection is mediated by a derivation towards pentose phosphate metabolic pathway and an increment of glutathione in androgen dependent prostate cancer cells. E-mail address: [email protected] (D. Hevia) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.319
P-235
A novel nutrient blend mimics calorie restriction transcriptomics differentially in multiple tissues of mice Angela Mastaloudis 1, Eva Serna 2, Steven M. Wood 1, Shelly Hester 1, Richard Weindruch 3,4,5, Tomas A. Prolla 3,6, Jose Vina 7 1
Nu Skin Enterprises, Pharmanex Research, Provo, UT, USA Central Research Unit-INCLIVA, Faculty of Medicine, University of Valencia, Valencia, Spain 3 LifeGen Technologies LLC, Madison, WI, USA 4 Department of Medicine, SMPH, University of Wisconsin, Madison, WI, USA 5 Geriatric Research, Education and Clinical Center, William S. Middleton Memorial Veterans Hospital, Madison, WI, USA 6 Departments of Genetics and Medical Genetics; University of Wisconsin; Madison, WI, USA 7 Department of Physiology, Faculty of Medicine, University of Valencia, INCLIVA, Valencia, Spain 2
Keywords: calorie restriction mimetic; transcriptomics; phytonutrients; anti-aging; nutrition
Identification of caloric restriction mimetics (CRMs), compounds that mimic the beneficial effects of caloric restriction (CR) without restriction of dietary energy would be an advancement in anti-aging science. The present study investigated whether the transcriptional profiles of a putative CRM nutrient blend could mimic that of CR in diverse tissues following long-term feeding in B6C3F1 male mice. Study design: Young Controls (YC; 5 mo.) and 3 groups treated from 14-30 mo.: Old Controls (OC), Old CR (OCR; 25% CR) and Old Supplemented (OS); n¼7/group. Gene expression profiling in cerebral cortex (CCT), skeletal muscle (gastrocnemius) (SKL), heart (HRT), white adipose tissue (WAT) and liver (LVR) was performed using Affymetrix Mouse 2.0ST arrays. Principal component analysis revealed that gene expression profiles of YC and OC were distinct from one another in all tissues at 30 months. Using differential analysis, genes commonly expressed in OCR and OS groups compared to the OC group were identified in CCT (3,468), SKL (2,386), HRT (3,523), LVR (1,276) and WAT (683). The OS group mimicked OCR transcriptional profiles most dramatically in CCT, HRT and SKL, tissues highly relevant to aging and age-associated diseases. These CRM effects, elicited by a mid-life intervention, may have positive implications for healthy human aging or ‘youthspan’ and warrant further investigation. E-mail address: [email protected] (A. Mastaloudis) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.320
Keywords: Antioxidant; ROS; Lemon balm; Polyphenol; Keratinocyte
Solar radiation exposure is the main cause of a variety of cutaneous disorders, including photoageing and skin cancers. Ultraviolet radiation (UV), especially UVB, causes DNA damage, pyrimidine dimmers, 8-hydroxy-2´-deoxyguanosine, p53 induction, protein oxidation and generation of reactive oxygen species (ROS) formation. Polyphenol species of Mediterranean plants have shown photoprotective effects. This study evaluated the protective action of lemon balm extract (LB) against UVB-induced damage in human keratinocytes (HaCaT cells) and correlates both activities. Antioxidant activity has been measured using up to four in vitro different assays (Folin-Ciocalteu assay, trolox equivalent antioxidant capacity, ferric reducing-antioxidant power and oxygen radical absorbance capacity assays). On the other hand, cell viability of HaCaT exposed to UVB was determined by MTT assay. Finally antioxidant activity by ROS-sensitive dichlorofluorescein diacetate was measured confirming the correlation between antioxidant and protective activities. In conclusion, our results suggest that LB extract present a potential photoprotective effect in human skin cell model against UVB-induced damage mediated by its antioxidant capacity, however, further research should be developed to elucidate molecular mechanisms. E-mail address: [email protected] (V. Ruiz-Torres) Acknowledgements
AGL2015-67995-C3-1-R from the Spanish Ministry of Science and Innovation, PROMETEO/2016/006 from GV, Instituto de Salud Carlos III and GV for VALi+D fellowships (ACIF/2015/158). http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.318
P-234
Glut 1 overexpression prevents glucose deprivationinduced prostate cancer cell death by increasing pentose phosphate pathway and glutathione David Hevia 1,2, Pedro Gonzalez-Menendez 1,2, Vanesa Cepas 1,2, Rosa M Sainz 1,2 1
Department of Morphology and Cell Biology, University of Oviedo University Institute of Oncology of Asturias (IUOPA), University of Oviedo, Spain
2
Keywords: prostate cancer; Glut 1; apoptosis; oxidative stress; glutathione
Although glucose metabolism was override in prostate cancer, it is recognized that there are metabolic differences between androgen-sensitive and castration-resistant phenotypes that might be responsible of progression or treatment success. Among the targets of glucose metabolism, glucose uptake is on the top of the cascade. The increase of glucose uptake by GLUTs overexpression in oncogenesis or the androgenic regulation of some GLUT transporters in other tissues is demonstrated. The prostate, at the beginning of carcinogenesis, depends on OXPHOS but then, at aggressive stages, tumors become again glycolytic like other cancers. Therefore, their resistance to glucose deprivation is different at the beginning or at later stages. In this work, we demonstrated by using androgen sensitive and insensitive cells that facilitative
S99
GLUT1 transporter overexpression protect prostate cancer cells from apoptosis caused by glucose removal. Glucose removal caused an increment in oxidative stress that stimulated androgen receptor activity and GLUT1 overexpression. This protection is mediated by a derivation towards pentose phosphate metabolic pathway and an increment of glutathione in androgen dependent prostate cancer cells. E-mail address: [email protected] (D. Hevia) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.319
P-235
A novel nutrient blend mimics calorie restriction transcriptomics differentially in multiple tissues of mice Angela Mastaloudis 1, Eva Serna 2, Steven M. Wood 1, Shelly Hester 1, Richard Weindruch 3,4,5, Tomas A. Prolla 3,6, Jose Vina 7 1
Nu Skin Enterprises, Pharmanex Research, Provo, UT, USA Central Research Unit-INCLIVA, Faculty of Medicine, University of Valencia, Valencia, Spain 3 LifeGen Technologies LLC, Madison, WI, USA 4 Department of Medicine, SMPH, University of Wisconsin, Madison, WI, USA 5 Geriatric Research, Education and Clinical Center, William S. Middleton Memorial Veterans Hospital, Madison, WI, USA 6 Departments of Genetics and Medical Genetics; University of Wisconsin; Madison, WI, USA 7 Department of Physiology, Faculty of Medicine, University of Valencia, INCLIVA, Valencia, Spain 2
Keywords: calorie restriction mimetic; transcriptomics; phytonutrients; anti-aging; nutrition
Identification of caloric restriction mimetics (CRMs), compounds that mimic the beneficial effects of caloric restriction (CR) without restriction of dietary energy would be an advancement in anti-aging science. The present study investigated whether the transcriptional profiles of a putative CRM nutrient blend could mimic that of CR in diverse tissues following long-term feeding in B6C3F1 male mice. Study design: Young Controls (YC; 5 mo.) and 3 groups treated from 14-30 mo.: Old Controls (OC), Old CR (OCR; 25% CR) and Old Supplemented (OS); n¼7/group. Gene expression profiling in cerebral cortex (CCT), skeletal muscle (gastrocnemius) (SKL), heart (HRT), white adipose tissue (WAT) and liver (LVR) was performed using Affymetrix Mouse 2.0ST arrays. Principal component analysis revealed that gene expression profiles of YC and OC were distinct from one another in all tissues at 30 months. Using differential analysis, genes commonly expressed in OCR and OS groups compared to the OC group were identified in CCT (3,468), SKL (2,386), HRT (3,523), LVR (1,276) and WAT (683). The OS group mimicked OCR transcriptional profiles most dramatically in CCT, HRT and SKL, tissues highly relevant to aging and age-associated diseases. These CRM effects, elicited by a mid-life intervention, may have positive implications for healthy human aging or ‘youthspan’ and warrant further investigation. E-mail address: [email protected] (A. Mastaloudis) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.320