- Email: [email protected]
The publication fate of male reproduction abstracts presented at the annual ASRM meeting
rats. In this work we provide additional clinical and molecular characterization of the effect of VitD3 on uterine fibroids. DESIGN: The therapeutic effect of VitD3 was examined in Eker rats spontaneous model for leiomyoma. MATERIALS AND METHODS: Leiomyomas in female Eker rats were confirmed by a limited laparotomy aseptically under anesthesia. Tumor-harboring rats were treated with VitD3 at the dose of 0.5 mg/Kg/day for 21 days. Rats were sacrificed, leiomyoma tumors were measured, and tissue samples were used for immunohistochemistry and western blots for molecular evaluation. RESULTS: VitD3 suppressed leiomyoma growth by reducing proliferation marker such as PCNA, Ki67, Cdk1, Cdk2, and Cdk4. Immunohistochemistry showed induced expression of caspase-3 by VitD3 treatment. Liver function analyses by measuring serum levels of SGPT, SGOT, and total bilirubin showed no significant changes between vehicle-control and VitD3treated rats. H&E staining of liver and kidney showed similar staining pattern between vehicle-control and VitD3-treated rats. Serum levels of calcium were similar between those two groups, suggesting the dose used is therapeutically safe and non-toxic. Together our results suggest the potent anti-tumor function of VitD3 on reduction of leiomyoma growth in Eker rats. CONCLUSION: VitD3 might be a potent and safe therapeutic agent for non-surgical treatment of leiomyoma. Supported by: NIH/NICHD R01 HD046228, RCMI grant G12RR03032 and 2G12 RR003032-26.
MALE REPRODUCTION AND UROLOGY: CLINICAL O-173 Tuesday, October 18, 2011 04:15 PM IS TESTICULAR SPERM MORE EFFICIENT THAN EPIDIDYMAL SPERM FOR ICSI IN PATIENTS WITH OBSTRUCTIVE AZOOSPERMIA? I. Hammoud, M. Albert, M. Bailly, F. Boitrelle, F. Vialard, J. Selva. ART Center, Institut Mutualiste Montsouris, Paris, France; ART Center, CHIPS, Poissy, France; EA 2493, University Versailles Saint Quentin en Yvelines, Versailles, France. OBJECTIVE: In obstructive azoospermia (OA), sperm DNA damages could be increased in epididymis more than in testis due to stagnation, or to higher pressure levels.The aim of this study was (i) to evaluate DNA fragmentation (DNA F) of surgically retrieved spermatozoa in different aetiologies of OA. DESIGN: Twenty one patients with OA and both epididymal and testicular sperm retrieved were included: 5 patients had congenital bilateral absence of vas deferens (CBAVD), 8 post-genital tract infections and 8 unknown aetiology. MATERIALS AND METHODS: Testicular spermatozoa were extracted by mechanical dilaceration and epididymal sperm cells were aspirated in a syringe. We compared the rates of DNA F of spermatozoa collected in the testis and the epididymis. Just after cryopreservation for ICSI, remaining spermatozoa were spread on slides and kept at -20 C. DNA F was evaluated by a TUNEL assay (Kit Roche Diagnostics, Milan, Italy) (normal value below 13%). RESULTS: 14 patients exhibited sperm both in testis biopsy, caput and corpus epididymis punctures and 7 patients had no sperm in corpus of epididymis. A total of 46526 spermatozoa (n ¼ 8288 in testis n ¼ 19358 in the epididymis caput, n ¼ 18880 in the epididymis corpus) DNA F rates at each level were the same whatever the aetiology. Testicular spermatozoa were less often DNA fragmented than epididymal ones (6.70 3.42% vs 23.89 % 5.89, P¼0.0009). Spermatozoa retrieved in epididymis caput were less often DNA fragmented than in the corpus (14.86 8.67% vs 32.92 3.12%; P¼0.04) CONCLUSION: Whatever was the aetiology of obstructive azoospermia, sperm DNA F rate was lower in testis than in epididymis. These results suggest that longer is the aging of spermatozoa after spermiation, higher is their risk to be DNA fragmented. Since epididymal sperm DNA F rates were above the normal cut-off, these data suggest that the use of testicular sperm in ICSI attempts should be counselled for patients with obstructive azoospermia.
undertaken to determine the publication rate among these abstracts, time to publication and predictive factors for publication. DESIGN: Cross Sectional Study. MATERIALS AND METHODS: All accepted abstracts related to male reproduction at the 2006 and 2007 ASRM meetings were reviewed. Comprehensive MEDLINE search was performed for evidence of publication in full manuscript form up to April 2011. Multivariate analyses were performed to determine predictive factors. RESULTS: 284 abstracts involving male reproduction were presented in 2006 and 2007. Of these, 107 (38%) achieved publication by April 2011 within a median followup of 16 months (range 10 to 28). Manuscripts were published in: Fertility & Sterility 29%, Journal of Urology 7%, Urology 6%. Publishing rates by category were: SMRU Traveling Scholars 55%, Male Reproductive Surgery 55%, Male Reproduction and Urology 50%, Sperm Biology 50%, Male Factor 32%. In multivariate analyses, longer time to publication was associated with academic department origin (urology vs urology & ob/gyn) [HR ¼ 0.46, 95%CI 0.25-0.82, P¼0.03], study design (prospective vs basic science) [HR ¼ 0.41, 95%CI 0.23-0.70, P¼0.02] and reporting of study duration (yes vs no) [HR ¼ 0.36, 95%CI 0.18-0.72, P<0.01]. Other variables such as category, country of origin, domain, # subjects, presentation type, report of statistical analysis and negative outcomes were not predictive. CONCLUSION: Nonpublication of research findings affects more than half of male reproductive studies 3 years after presentation at the ASRM. SMRU Traveling Scholar and Male Reproductive Surgery abstracts were published at the highest rate. The most significant predictors of rapid publication are study design type, academic department origin and study duration reporting. Continued efforts should be made to increase publication of male fertility related abstracts presented at the ASRM. O-175 Tuesday, October 18, 2011 04:45 PM LOW TOTAL MOTILE SPERM COUNT (TMC): SHOULD THE COUPLE UNDERGO INTRAUTERINE INSEMINATION (IUI) OR MOVE ON TO IN VITRO FERTILIZATION? S. Bullock, B. Storer, J. D. Peck, K. R. Hansen, L. B. Craig. Department of Obstetrics & Gynecology, Section of Reproductive Endocrinology & Infertility, University of Oklahoma Health Sciences Center, Oklahoma City, OK; Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, Oklahoma City, OK. OBJECTIVE: To determine if low total motile sperm count (TMC) negatively correlates with fecundability in women undergoing intrauterine insemination (IUI) with partner’s semen. DESIGN: A retrospective evaluation of patients undergoing IUI with their partner’s semen between July 2007 and June 2010 at a university-based infertility clinic. MATERIALS AND METHODS: Charts were reviewed for multiple factors known to influence pregnancy rates. IUI specimen parameters were recorded. The primary outcome was positive pregnancy test per cycle. We calculated risk ratios (RR) and 95% confidence intervals (95% CI) using a generalized estimating equation method to estimate Poisson regression models with robust standard errors to account for correlated observations due to multiple cycles in same patient. RESULTS: Of the 1,022 IUI cycles performed with partner’s sample, 9 cycles excluded due to missing charts leaving 1013 IUI cycles performed on 385 women (average age 32.2 5.2) for analysis. The overall pregnancy rate per cycle was 13.8 %. The numbers of subjects in each TMC category, as well as pregnancy rates are listed below. Overall, there was no association between TMC and pregnancy rates. Results were unchanged when adjusting for confounding factors: female age, female BMI, infertility duration, infertility diagnosis, and medication. If restricted to those with %2 million TMC, pregnancy was achieved in 10.5% of cycles (6/57). The lowest TMC resulting in pregnancy was 1.05 million. RESULTS:
O-174 Tuesday, October 18, 2011 04:30 PM
TMC x 106
# Cycles
Pregnancies (%)
RR
95% CI
THE PUBLICATION FATE OF MALE REPRODUCTION ABSTRACTS PRESENTED AT THE ANNUAL ASRM MEETING. R. P. Bonitz, T. Nyirenda, H. Lowe, D. Shin. Department of Urology, Hackensack University Medical Center, Hackensack, NJ; Department of Research, Hackensack University Medical Center, Hackensack, NJ; Division of Urology, UMDNJ-New Jersey Medical School, Newark, NJ.
% 5.0 5.1-10.0 10.1-20.0 20.1-30.0 >30
188 214 267 140 204
19 (10.1) 26 (12.2) 41 (15.4) 23 (16.4) 31 (15.2)
0.8 0.8 1.0 1.1 1.0
0.5-1.5 0.5-1.4 0.6-1.4 0.6-1.8 Reference
OBJECTIVE: Over 1100 abstracts are presented at the annual ASRM meeting, of which 11% involve male reproductive urology. This study was
CONCLUSION: In contrast to previous studies showing a negative correlation of fecundability with TMC, pregnancy rates in women undergoing IUI
S52
Abstracts
Vol. 96., No. 3, Supplement, September 2011
MALE REPRODUCTION AND UROLOGY: CLINICAL O-173 Tuesday, October 18, 2011 04:15 PM IS TESTICULAR SPERM MORE EFFICIENT THAN EPIDIDYMAL SPERM FOR ICSI IN PATIENTS WITH OBSTRUCTIVE AZOOSPERMIA? I. Hammoud, M. Albert, M. Bailly, F. Boitrelle, F. Vialard, J. Selva. ART Center, Institut Mutualiste Montsouris, Paris, France; ART Center, CHIPS, Poissy, France; EA 2493, University Versailles Saint Quentin en Yvelines, Versailles, France. OBJECTIVE: In obstructive azoospermia (OA), sperm DNA damages could be increased in epididymis more than in testis due to stagnation, or to higher pressure levels.The aim of this study was (i) to evaluate DNA fragmentation (DNA F) of surgically retrieved spermatozoa in different aetiologies of OA. DESIGN: Twenty one patients with OA and both epididymal and testicular sperm retrieved were included: 5 patients had congenital bilateral absence of vas deferens (CBAVD), 8 post-genital tract infections and 8 unknown aetiology. MATERIALS AND METHODS: Testicular spermatozoa were extracted by mechanical dilaceration and epididymal sperm cells were aspirated in a syringe. We compared the rates of DNA F of spermatozoa collected in the testis and the epididymis. Just after cryopreservation for ICSI, remaining spermatozoa were spread on slides and kept at -20 C. DNA F was evaluated by a TUNEL assay (Kit Roche Diagnostics, Milan, Italy) (normal value below 13%). RESULTS: 14 patients exhibited sperm both in testis biopsy, caput and corpus epididymis punctures and 7 patients had no sperm in corpus of epididymis. A total of 46526 spermatozoa (n ¼ 8288 in testis n ¼ 19358 in the epididymis caput, n ¼ 18880 in the epididymis corpus) DNA F rates at each level were the same whatever the aetiology. Testicular spermatozoa were less often DNA fragmented than epididymal ones (6.70 3.42% vs 23.89 % 5.89, P¼0.0009). Spermatozoa retrieved in epididymis caput were less often DNA fragmented than in the corpus (14.86 8.67% vs 32.92 3.12%; P¼0.04) CONCLUSION: Whatever was the aetiology of obstructive azoospermia, sperm DNA F rate was lower in testis than in epididymis. These results suggest that longer is the aging of spermatozoa after spermiation, higher is their risk to be DNA fragmented. Since epididymal sperm DNA F rates were above the normal cut-off, these data suggest that the use of testicular sperm in ICSI attempts should be counselled for patients with obstructive azoospermia.
undertaken to determine the publication rate among these abstracts, time to publication and predictive factors for publication. DESIGN: Cross Sectional Study. MATERIALS AND METHODS: All accepted abstracts related to male reproduction at the 2006 and 2007 ASRM meetings were reviewed. Comprehensive MEDLINE search was performed for evidence of publication in full manuscript form up to April 2011. Multivariate analyses were performed to determine predictive factors. RESULTS: 284 abstracts involving male reproduction were presented in 2006 and 2007. Of these, 107 (38%) achieved publication by April 2011 within a median followup of 16 months (range 10 to 28). Manuscripts were published in: Fertility & Sterility 29%, Journal of Urology 7%, Urology 6%. Publishing rates by category were: SMRU Traveling Scholars 55%, Male Reproductive Surgery 55%, Male Reproduction and Urology 50%, Sperm Biology 50%, Male Factor 32%. In multivariate analyses, longer time to publication was associated with academic department origin (urology vs urology & ob/gyn) [HR ¼ 0.46, 95%CI 0.25-0.82, P¼0.03], study design (prospective vs basic science) [HR ¼ 0.41, 95%CI 0.23-0.70, P¼0.02] and reporting of study duration (yes vs no) [HR ¼ 0.36, 95%CI 0.18-0.72, P<0.01]. Other variables such as category, country of origin, domain, # subjects, presentation type, report of statistical analysis and negative outcomes were not predictive. CONCLUSION: Nonpublication of research findings affects more than half of male reproductive studies 3 years after presentation at the ASRM. SMRU Traveling Scholar and Male Reproductive Surgery abstracts were published at the highest rate. The most significant predictors of rapid publication are study design type, academic department origin and study duration reporting. Continued efforts should be made to increase publication of male fertility related abstracts presented at the ASRM. O-175 Tuesday, October 18, 2011 04:45 PM LOW TOTAL MOTILE SPERM COUNT (TMC): SHOULD THE COUPLE UNDERGO INTRAUTERINE INSEMINATION (IUI) OR MOVE ON TO IN VITRO FERTILIZATION? S. Bullock, B. Storer, J. D. Peck, K. R. Hansen, L. B. Craig. Department of Obstetrics & Gynecology, Section of Reproductive Endocrinology & Infertility, University of Oklahoma Health Sciences Center, Oklahoma City, OK; Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, Oklahoma City, OK. OBJECTIVE: To determine if low total motile sperm count (TMC) negatively correlates with fecundability in women undergoing intrauterine insemination (IUI) with partner’s semen. DESIGN: A retrospective evaluation of patients undergoing IUI with their partner’s semen between July 2007 and June 2010 at a university-based infertility clinic. MATERIALS AND METHODS: Charts were reviewed for multiple factors known to influence pregnancy rates. IUI specimen parameters were recorded. The primary outcome was positive pregnancy test per cycle. We calculated risk ratios (RR) and 95% confidence intervals (95% CI) using a generalized estimating equation method to estimate Poisson regression models with robust standard errors to account for correlated observations due to multiple cycles in same patient. RESULTS: Of the 1,022 IUI cycles performed with partner’s sample, 9 cycles excluded due to missing charts leaving 1013 IUI cycles performed on 385 women (average age 32.2 5.2) for analysis. The overall pregnancy rate per cycle was 13.8 %. The numbers of subjects in each TMC category, as well as pregnancy rates are listed below. Overall, there was no association between TMC and pregnancy rates. Results were unchanged when adjusting for confounding factors: female age, female BMI, infertility duration, infertility diagnosis, and medication. If restricted to those with %2 million TMC, pregnancy was achieved in 10.5% of cycles (6/57). The lowest TMC resulting in pregnancy was 1.05 million. RESULTS:
O-174 Tuesday, October 18, 2011 04:30 PM
TMC x 106
# Cycles
Pregnancies (%)
RR
95% CI
THE PUBLICATION FATE OF MALE REPRODUCTION ABSTRACTS PRESENTED AT THE ANNUAL ASRM MEETING. R. P. Bonitz, T. Nyirenda, H. Lowe, D. Shin. Department of Urology, Hackensack University Medical Center, Hackensack, NJ; Department of Research, Hackensack University Medical Center, Hackensack, NJ; Division of Urology, UMDNJ-New Jersey Medical School, Newark, NJ.
% 5.0 5.1-10.0 10.1-20.0 20.1-30.0 >30
188 214 267 140 204
19 (10.1) 26 (12.2) 41 (15.4) 23 (16.4) 31 (15.2)
0.8 0.8 1.0 1.1 1.0
0.5-1.5 0.5-1.4 0.6-1.4 0.6-1.8 Reference
OBJECTIVE: Over 1100 abstracts are presented at the annual ASRM meeting, of which 11% involve male reproductive urology. This study was
CONCLUSION: In contrast to previous studies showing a negative correlation of fecundability with TMC, pregnancy rates in women undergoing IUI
S52
Abstracts
Vol. 96., No. 3, Supplement, September 2011