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The role of adenosine receptors in the human breast cancers: a decade of experience
S70
Abstracts
a
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Zand Blvd, Shiraz, Iran b Department of Biochemistry, School of Medicine, Yasuj, Iran c Department of Internal Medicine, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran d Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Zand Blvd, Shiraz, Iran E-mail addresses: [email protected], [email protected] (S.S. Mohammad), [email protected] (S. Atefeh), [email protected] (N. Mohsen), [email protected] (M. Ahmad), [email protected] (O. Aliakbar) Introduction: Colorectal cancer is the third most common cancer in the world. UBE2Q1 is a novel human gene that encodes a putative E2 ubiquitin conjugating enzyme. Ubiquitin–proteasome pathway has been shown to be activated in colorectal and other malignancies. Here, we investigated the expression pattern of UBE2Q1 gene in 8 colorectal cancerous cell lines and in 3 human colorectal tumor samples. Methods: Colorectal cancer cell lines (HT29/219, LS180, SW742, Caco2, HTC116, SW48, SW480 and SW1116) along with colorectal tumor samples were subjected to western blotting and Real Time PCR to evaluate the expression levels of UBE2Q1. Results: All of the cell lines expressed UBE2Q1 gene at the level of both mRNA and protein, among them, SW742 and SW1116 cell lines represented the highest and the lowest levels of expression, respectively. The expression levels of UBE2Q1 protein in cancerous samples were higher than their adjacent normal tissues. Conclusions: In this study, the expression of UBE2Q1 gene in colorectal cell lines and cancerous tissue was shown for the first time. Based on data obtained, UBE2Q1 is differentially expressed at mRNA and protein levels in colorectal cancer proposing a molecular marker for diagnosis and treatment of cancer in the future.
expression. Taken together, down-regulation of WEE1 may enhance the endogenous immune response against brain tumors by suppressing the expression of anti-inflammatory cytokines, IL10 and IL13. Keywords: WEE1 kinase, Glioma, Small hairpin RNA, IL10, IL13 doi:10.1016/j.clinbiochem.2011.08.150
Oral – [A-10-481-1] The role of adenosine receptors in the human breast cancers: a decade of experience Panjehpour Mojtaba Biochemistry, School of Pharmacy, Bioinformatics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran E-mail address: [email protected] Introduction: It is of enormous interest to identify factors, which predispose the normal breast tissue to severe and metastatic form, and to find novel ways to treat metastatic breast cancer. My studies over the last several years have revealed the functional role of adenosine receptors on the cellular mitogenesis and activation of cell signaling machinery linked to these membrane receptors. The adenosine receptors belong to the family of GPCRs and classified into four subtypes (A1, A2A, A2B and A3). It is recognized that the concentrations of adenosine are increased in cancer tissues. This is of particular interest, because the regulation of angiogenesis and metastasis is related to the high levels of adenosine. Therefore, it is interesting to clarify the expression profile, signal transduction, molecular function and cell growth modulation of adenosine receptor subtypes in the human breast cancer cell lines as well as differential expression and possible roles of adenosine receptors in the breast tumors versus normal tissues.
Keywords: Colorectal cancer, UBE2Q1 gene
Keywords: Adenosine receptors, Breast cancer
doi:10.1016/j.clinbiochem.2011.08.149
doi:10.1016/j.clinbiochem.2011.08.151
E Poster – [A-10-395-2] The inhibition of anti-inflammatory cytokines in glioblastoma cell lines by WEE1 gene silencing with specific ShRNA Jaberipour Mansooreha, Rahnama Susanb, Hosseini Ahmada, Habibagahi Mojtabac a Shiraz, Zand St, Medical School, Institute for Cancer Research, Iran b Shiraz, Medical School,Department of Immunology, Iran c Shiraz, Zand St, Medical School, Department of Immunology, Iran E-mail addresses: [email protected] (J. Mansooreh), [email protected] (R. Susan), [email protected] (H. Ahmad), [email protected] (H. Mojtaba)
Oral – [A-10-490-1] Analysis of gene expression profile changes in response to hypoxia using cDNA-AFLP as a differential display method in two human glioma cell lines (U87, A172) Fatemeh Baghbania, Majid Mojarrada, Tayebeh Hamzehloeia, Mansour Heidarib, Reza Raoofiana, Mohammad Hasanzadeh Nazar-Abadia, Mohammad Soukhtanlooa, Sahar Shekouhia a Mashhad University of Medical Sciences, Faculty of Medicine, Department of Medical Genetics, Mashhad, Iran b Tehran University of Medical Sciences, Faculty of Medicine, Department of Medical Genetics, Iran E-mail addresses: [email protected] (F. Baghbani), [email protected] (M. Mojarrad), [email protected] (T. Hamzehloei), [email protected] (M. Heidari), [email protected] (R. Raoofian), [email protected] (M.H. Nazar-Abadi), [email protected] (M. Soukhtanloo), [email protected] (S. Shekouhi)
Introduction: Glioma is the most common central nervous system (CNS) neoplasm in human with poor prognosis. Understanding the role of different genes can provide new therapeutic approaches. In this regard, the biological function of WEE1 may underline this gene as a potential target in brain tumors. In this study, the effect of WEE1 gene silencing on the expression of IL10, IL13 and IL13R was assessed as this factors show prominent effect on tumor progression and metastasis. Material and methods: By RNAi technology, WEE1 gene was silenced in U373 and A172 brain tumor cell lines using lipofectamin 2000 and plasmids expressing gene specific shRNA. The WEE1 gene silencing was confirmed by real-time PCR and western blotting. The expression levels of IL10, IL13, IL13R and β-actin were determined using Real-time PCR and TaqMan probes. WEE1 gene silencing resulted in simultaneous decreased expression of IL10 and IL13 compared to untreated cells. However, inhibition of WEE1 did not affect the IL13R
Introduction: Glioblastoma multiform is the most common, aggressive and malignant primary brain tumor with no effective cure so far, and poor prognosis. One of the reasons for this poor prognosis is the extreme sensitivity of tumor cells to hypoxia. Methods: Two glioma cell lines (U87, A172) were cultured in hypoxia condition (1% O2). RNA was extracted and converted into cDNA. Using Klenow DNA polymerase, cDNA was converted to double stranded DNA. DNA was digested using MspI and MseI restriction
Abstracts
a
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Zand Blvd, Shiraz, Iran b Department of Biochemistry, School of Medicine, Yasuj, Iran c Department of Internal Medicine, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran d Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Zand Blvd, Shiraz, Iran E-mail addresses: [email protected], [email protected] (S.S. Mohammad), [email protected] (S. Atefeh), [email protected] (N. Mohsen), [email protected] (M. Ahmad), [email protected] (O. Aliakbar) Introduction: Colorectal cancer is the third most common cancer in the world. UBE2Q1 is a novel human gene that encodes a putative E2 ubiquitin conjugating enzyme. Ubiquitin–proteasome pathway has been shown to be activated in colorectal and other malignancies. Here, we investigated the expression pattern of UBE2Q1 gene in 8 colorectal cancerous cell lines and in 3 human colorectal tumor samples. Methods: Colorectal cancer cell lines (HT29/219, LS180, SW742, Caco2, HTC116, SW48, SW480 and SW1116) along with colorectal tumor samples were subjected to western blotting and Real Time PCR to evaluate the expression levels of UBE2Q1. Results: All of the cell lines expressed UBE2Q1 gene at the level of both mRNA and protein, among them, SW742 and SW1116 cell lines represented the highest and the lowest levels of expression, respectively. The expression levels of UBE2Q1 protein in cancerous samples were higher than their adjacent normal tissues. Conclusions: In this study, the expression of UBE2Q1 gene in colorectal cell lines and cancerous tissue was shown for the first time. Based on data obtained, UBE2Q1 is differentially expressed at mRNA and protein levels in colorectal cancer proposing a molecular marker for diagnosis and treatment of cancer in the future.
expression. Taken together, down-regulation of WEE1 may enhance the endogenous immune response against brain tumors by suppressing the expression of anti-inflammatory cytokines, IL10 and IL13. Keywords: WEE1 kinase, Glioma, Small hairpin RNA, IL10, IL13 doi:10.1016/j.clinbiochem.2011.08.150
Oral – [A-10-481-1] The role of adenosine receptors in the human breast cancers: a decade of experience Panjehpour Mojtaba Biochemistry, School of Pharmacy, Bioinformatics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran E-mail address: [email protected] Introduction: It is of enormous interest to identify factors, which predispose the normal breast tissue to severe and metastatic form, and to find novel ways to treat metastatic breast cancer. My studies over the last several years have revealed the functional role of adenosine receptors on the cellular mitogenesis and activation of cell signaling machinery linked to these membrane receptors. The adenosine receptors belong to the family of GPCRs and classified into four subtypes (A1, A2A, A2B and A3). It is recognized that the concentrations of adenosine are increased in cancer tissues. This is of particular interest, because the regulation of angiogenesis and metastasis is related to the high levels of adenosine. Therefore, it is interesting to clarify the expression profile, signal transduction, molecular function and cell growth modulation of adenosine receptor subtypes in the human breast cancer cell lines as well as differential expression and possible roles of adenosine receptors in the breast tumors versus normal tissues.
Keywords: Colorectal cancer, UBE2Q1 gene
Keywords: Adenosine receptors, Breast cancer
doi:10.1016/j.clinbiochem.2011.08.149
doi:10.1016/j.clinbiochem.2011.08.151
E Poster – [A-10-395-2] The inhibition of anti-inflammatory cytokines in glioblastoma cell lines by WEE1 gene silencing with specific ShRNA Jaberipour Mansooreha, Rahnama Susanb, Hosseini Ahmada, Habibagahi Mojtabac a Shiraz, Zand St, Medical School, Institute for Cancer Research, Iran b Shiraz, Medical School,Department of Immunology, Iran c Shiraz, Zand St, Medical School, Department of Immunology, Iran E-mail addresses: [email protected] (J. Mansooreh), [email protected] (R. Susan), [email protected] (H. Ahmad), [email protected] (H. Mojtaba)
Oral – [A-10-490-1] Analysis of gene expression profile changes in response to hypoxia using cDNA-AFLP as a differential display method in two human glioma cell lines (U87, A172) Fatemeh Baghbania, Majid Mojarrada, Tayebeh Hamzehloeia, Mansour Heidarib, Reza Raoofiana, Mohammad Hasanzadeh Nazar-Abadia, Mohammad Soukhtanlooa, Sahar Shekouhia a Mashhad University of Medical Sciences, Faculty of Medicine, Department of Medical Genetics, Mashhad, Iran b Tehran University of Medical Sciences, Faculty of Medicine, Department of Medical Genetics, Iran E-mail addresses: [email protected] (F. Baghbani), [email protected] (M. Mojarrad), [email protected] (T. Hamzehloei), [email protected] (M. Heidari), [email protected] (R. Raoofian), [email protected] (M.H. Nazar-Abadi), [email protected] (M. Soukhtanloo), [email protected] (S. Shekouhi)
Introduction: Glioma is the most common central nervous system (CNS) neoplasm in human with poor prognosis. Understanding the role of different genes can provide new therapeutic approaches. In this regard, the biological function of WEE1 may underline this gene as a potential target in brain tumors. In this study, the effect of WEE1 gene silencing on the expression of IL10, IL13 and IL13R was assessed as this factors show prominent effect on tumor progression and metastasis. Material and methods: By RNAi technology, WEE1 gene was silenced in U373 and A172 brain tumor cell lines using lipofectamin 2000 and plasmids expressing gene specific shRNA. The WEE1 gene silencing was confirmed by real-time PCR and western blotting. The expression levels of IL10, IL13, IL13R and β-actin were determined using Real-time PCR and TaqMan probes. WEE1 gene silencing resulted in simultaneous decreased expression of IL10 and IL13 compared to untreated cells. However, inhibition of WEE1 did not affect the IL13R
Introduction: Glioblastoma multiform is the most common, aggressive and malignant primary brain tumor with no effective cure so far, and poor prognosis. One of the reasons for this poor prognosis is the extreme sensitivity of tumor cells to hypoxia. Methods: Two glioma cell lines (U87, A172) were cultured in hypoxia condition (1% O2). RNA was extracted and converted into cDNA. Using Klenow DNA polymerase, cDNA was converted to double stranded DNA. DNA was digested using MspI and MseI restriction