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The role of tissue colonization and bacterial resistance in recurrent tonsillitis
216
Abstracts
selected
jiom
EMBASE
response (ABR) assessmentsduring the same period. One group was considered otitis media (OM)-positive, with repeated bilateral episodes of OM and mild conductive hearing loss. The second group was considered OM-free with normal middle ear function bilaterally and normal hearing sensitivity in the first year of life. Children’s higher-order auditory abilities were examined at 4, 6 and 9 years of age. Various measures such as speech recognition-in-competition, visual-auditory learning, sound blending, auditory memory, and masking level difference estimates were utilized to probe the children’s auditory abilities. Results show that children with a first-year history of otitis media demonstrate deficits in the longer term in some aspects of higher-order auditory processing. Performance appears to be associated with the mild hearing loss experienced during an important period of early development. The role of tissue colonization and bacterial resistance in recurrent tonsillitis Mevio E.; Perano D.; Pagani L.; Zanella C.; Giacobone E.; Cardillo A. ACTA OTO-LARYNGOL. SUPPL. (ITA) (1996) -15.23 (133- 137)
Unsuccessful medical therapy for treatment of acute tonsillitis frequently results in onset of recurrent or chronic forms rendering surgical treatment necessary. We have studied some of the factors involved in the evolution of these chronic or recurrent forms and, in particular, the distribution of different bacterial strains in tonsillar tissue, their response to treatment with antibiotics and mechanisms of bacterial resistance. The distribution of bacterial flora (saprophytic and pathogenic) present on the surface of the tonsils, in the crypts and in the tonsillar tissue was studied in a control population of 30 children (20 boys and 10 girls) aged between 2- 13 years, all affected with chronic tonsillitis and submitted to tonsillectomy. The same study was performed in a group of 80 sex and age-matched children, also presenting with chronic or recurrent tonsillitis and treated with amoxicillin or amoxicillinclavulanic acid or cefaclor or clarithromycin 72 h before surgery. The 80 subjects were randomly divided into four groups so that each antibiotic was tested on 20 subjects. The distribution of the bacterial population on the surface of tonsillar tissue, in the crypts and in the deeper tonsillar tissue is of particular interest concerning the affinity of bacteria to the different tissue areas. In particular the interaction between crypt and tonsillar core, which could be a factor involved in the process of worsening of bacteria1 infection in the tonsils, is evidenced. The four antibodies tested showed different abilities to eradicate infection: Haemophilus influenzae was found to be the most resistant germ to antibiotic therapy and was thus the most frequent cause of recurrent infections. The characteristics and the mechanisms of adherence and resistance to lactam antibiotics were also analysed. Adenoid lymphocyte responses to outer membrane protein P6 of nontypable children with and without otitis media Kodama H.; Faden H.; Harabuchi Y.; Kataura A.; Bernstein J.M.; Brodsky ACTA OTO-LARYNGOL. SUPPL. (USA) (1996) -1523 (153-154)
Haemophilus
influenzae
in
L.
The cellular immune responses to nontypable Haemophilus influenzae in adenoids were determined in children by measuring lymphocyte blast transformation and antibody secretion in response to the P6 outer membrane protein. In the lymphocyte transformation assay, stimulation index of adenoid lymphocytes stimulated by P6 in otitis children (2.34 f 0.25) was significantly lower than that in non-otitis children (3.91 k 0.64, p < 0.05). The number of IgM as well as IgA secreting cells after 8 days’ culture with P6 were significantly smaller in otitis children (IgM: 7534 + 2843/106 lymphocyte; IgA: 1573 k 620/106 lymphocyte) than those in non-otitis children (IgM: 12994 k 2533, p < 0.05; IgA: 2828 + 528, p < 0.05). These data suggest that P6 protein is a target for the cellular immune response of the adenoid, and failure of adenoid lymphocytes to recognize it as a specific immunogen may be one of the causes of recurrent otitis media. Secretory IgA and squamous epitbelization in adenoids of children with Fujihara K.; Fujihara T.; Yamanaka N. ACTA OTO-LAR YNGOL. SUPPL. (JPN) (1996) -1523 (155- 157)
otitis
media
with
effusion
Infection of adenoids has been considered a causative factor of otitis media with effusion (OME). We believe that the deterioration in the mucosal barrier of adenoids makes the adenoids vulnerable to bacterial infections, resulting OME. In this study, we evaluated secretory IgA, secretory (SC) and reticular and squamous changes of ciliated epithelium in adenoids. Fourteen children with OME
Abstracts
selected
jiom
EMBASE
response (ABR) assessmentsduring the same period. One group was considered otitis media (OM)-positive, with repeated bilateral episodes of OM and mild conductive hearing loss. The second group was considered OM-free with normal middle ear function bilaterally and normal hearing sensitivity in the first year of life. Children’s higher-order auditory abilities were examined at 4, 6 and 9 years of age. Various measures such as speech recognition-in-competition, visual-auditory learning, sound blending, auditory memory, and masking level difference estimates were utilized to probe the children’s auditory abilities. Results show that children with a first-year history of otitis media demonstrate deficits in the longer term in some aspects of higher-order auditory processing. Performance appears to be associated with the mild hearing loss experienced during an important period of early development. The role of tissue colonization and bacterial resistance in recurrent tonsillitis Mevio E.; Perano D.; Pagani L.; Zanella C.; Giacobone E.; Cardillo A. ACTA OTO-LARYNGOL. SUPPL. (ITA) (1996) -15.23 (133- 137)
Unsuccessful medical therapy for treatment of acute tonsillitis frequently results in onset of recurrent or chronic forms rendering surgical treatment necessary. We have studied some of the factors involved in the evolution of these chronic or recurrent forms and, in particular, the distribution of different bacterial strains in tonsillar tissue, their response to treatment with antibiotics and mechanisms of bacterial resistance. The distribution of bacterial flora (saprophytic and pathogenic) present on the surface of the tonsils, in the crypts and in the tonsillar tissue was studied in a control population of 30 children (20 boys and 10 girls) aged between 2- 13 years, all affected with chronic tonsillitis and submitted to tonsillectomy. The same study was performed in a group of 80 sex and age-matched children, also presenting with chronic or recurrent tonsillitis and treated with amoxicillin or amoxicillinclavulanic acid or cefaclor or clarithromycin 72 h before surgery. The 80 subjects were randomly divided into four groups so that each antibiotic was tested on 20 subjects. The distribution of the bacterial population on the surface of tonsillar tissue, in the crypts and in the deeper tonsillar tissue is of particular interest concerning the affinity of bacteria to the different tissue areas. In particular the interaction between crypt and tonsillar core, which could be a factor involved in the process of worsening of bacteria1 infection in the tonsils, is evidenced. The four antibodies tested showed different abilities to eradicate infection: Haemophilus influenzae was found to be the most resistant germ to antibiotic therapy and was thus the most frequent cause of recurrent infections. The characteristics and the mechanisms of adherence and resistance to lactam antibiotics were also analysed. Adenoid lymphocyte responses to outer membrane protein P6 of nontypable children with and without otitis media Kodama H.; Faden H.; Harabuchi Y.; Kataura A.; Bernstein J.M.; Brodsky ACTA OTO-LARYNGOL. SUPPL. (USA) (1996) -1523 (153-154)
Haemophilus
influenzae
in
L.
The cellular immune responses to nontypable Haemophilus influenzae in adenoids were determined in children by measuring lymphocyte blast transformation and antibody secretion in response to the P6 outer membrane protein. In the lymphocyte transformation assay, stimulation index of adenoid lymphocytes stimulated by P6 in otitis children (2.34 f 0.25) was significantly lower than that in non-otitis children (3.91 k 0.64, p < 0.05). The number of IgM as well as IgA secreting cells after 8 days’ culture with P6 were significantly smaller in otitis children (IgM: 7534 + 2843/106 lymphocyte; IgA: 1573 k 620/106 lymphocyte) than those in non-otitis children (IgM: 12994 k 2533, p < 0.05; IgA: 2828 + 528, p < 0.05). These data suggest that P6 protein is a target for the cellular immune response of the adenoid, and failure of adenoid lymphocytes to recognize it as a specific immunogen may be one of the causes of recurrent otitis media. Secretory IgA and squamous epitbelization in adenoids of children with Fujihara K.; Fujihara T.; Yamanaka N. ACTA OTO-LAR YNGOL. SUPPL. (JPN) (1996) -1523 (155- 157)
otitis
media
with
effusion
Infection of adenoids has been considered a causative factor of otitis media with effusion (OME). We believe that the deterioration in the mucosal barrier of adenoids makes the adenoids vulnerable to bacterial infections, resulting OME. In this study, we evaluated secretory IgA, secretory (SC) and reticular and squamous changes of ciliated epithelium in adenoids. Fourteen children with OME