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THE STUDY OF CORRELATION WITH BETA2-ADRENERGIC RECEPTOR GENE POLYMORPHISMS AND HEART FAILURE
October 2007, Vol 132, No. 4_MeetingAbstracts Abstract: Slide Presentations | October 2007
THE STUDY OF CORRELATION WITH BETA2-ADRENERGIC RECEPTOR GENE POLYMORPHISMS AND HEART FAILURE Xumin Hou, PhD*; Weiyi Fang, PhD Shanghai Chest Hospital, Cardiology Department, Shanghai, Peoples Rep of China Chest Chest. 2007;132(4_MeetingAbstracts):477a. doi:10.1378/chest.132.4_MeetingAbstracts.477a
Abstract PURPOSE: To investigate the association between the β2-adrenergic receptor gene polymorphisms and chronic heart failure (CHF) in Southern Chinese Han population. METHODS: Genomic DNA was extracted from blood leukocytes from patients with CHF (n=92) and from healthy controls (n=80). The part of the β2-adrenergic receptor gene was amplified by polymerase chain reaction (PCR) and analysed by automated sequencing. RESULTS: There were four single nucleotide polymorphisms (SNPs) of β2-adrenergic receptor gene: 16Arg/Gly, 27Gln/Glu, 87LeuA/G and 178ArgA/C. Mono-locus analysis showed the genotype of 27 Gln/Glu were all C/C in controls, while there were seven G/C in CHF patients, no G/G were found. The difference of genotype and allele were significant between two groups (P<0.01). There was no significant difference between two groups of other SNPs (P>0.05). From the clinical data analysis of CHF patients, LVEF was worse in G/C genotype group than in C/C group (39% versus 51%, respectively; P=0.004), the proportion of NYHA 4 was more in G/C genotype group than in C/C group (57.1% versus 18.5%, respectively; P=0.04). The haplotype analysis showed the frequency of 16G-27C-87G in CHF patients was significantly decreased as compared with controls (0.095 versus 0.191, respectively; OR, 0.445 [95%CI, 0.277 to 0.715]; P<0.01). CONCLUSION: The 27Gln/Glu heterozygous is probably a susceptible genotype of CHF, which is related to the severity of CHF and left ventricular function. The 16G-27C-87G is probably a protecting haplotype of CHF. CLINICAL IMPLICATIONS: The β2-adrenergic receptor gene polymorphisms was associated with CHF in Southern Chinese Han population. DISCLOSURE: Xumin Hou, No Financial Disclosure Information; No Product/Research Disclosure Information Tuesday, October 23, 2007
2:30 PM - 4:00 PM
THE STUDY OF CORRELATION WITH BETA2-ADRENERGIC RECEPTOR GENE POLYMORPHISMS AND HEART FAILURE Xumin Hou, PhD*; Weiyi Fang, PhD Shanghai Chest Hospital, Cardiology Department, Shanghai, Peoples Rep of China Chest Chest. 2007;132(4_MeetingAbstracts):477a. doi:10.1378/chest.132.4_MeetingAbstracts.477a
Abstract PURPOSE: To investigate the association between the β2-adrenergic receptor gene polymorphisms and chronic heart failure (CHF) in Southern Chinese Han population. METHODS: Genomic DNA was extracted from blood leukocytes from patients with CHF (n=92) and from healthy controls (n=80). The part of the β2-adrenergic receptor gene was amplified by polymerase chain reaction (PCR) and analysed by automated sequencing. RESULTS: There were four single nucleotide polymorphisms (SNPs) of β2-adrenergic receptor gene: 16Arg/Gly, 27Gln/Glu, 87LeuA/G and 178ArgA/C. Mono-locus analysis showed the genotype of 27 Gln/Glu were all C/C in controls, while there were seven G/C in CHF patients, no G/G were found. The difference of genotype and allele were significant between two groups (P<0.01). There was no significant difference between two groups of other SNPs (P>0.05). From the clinical data analysis of CHF patients, LVEF was worse in G/C genotype group than in C/C group (39% versus 51%, respectively; P=0.004), the proportion of NYHA 4 was more in G/C genotype group than in C/C group (57.1% versus 18.5%, respectively; P=0.04). The haplotype analysis showed the frequency of 16G-27C-87G in CHF patients was significantly decreased as compared with controls (0.095 versus 0.191, respectively; OR, 0.445 [95%CI, 0.277 to 0.715]; P<0.01). CONCLUSION: The 27Gln/Glu heterozygous is probably a susceptible genotype of CHF, which is related to the severity of CHF and left ventricular function. The 16G-27C-87G is probably a protecting haplotype of CHF. CLINICAL IMPLICATIONS: The β2-adrenergic receptor gene polymorphisms was associated with CHF in Southern Chinese Han population. DISCLOSURE: Xumin Hou, No Financial Disclosure Information; No Product/Research Disclosure Information Tuesday, October 23, 2007
2:30 PM - 4:00 PM