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The treatments effect with pesticides at canola on honeybees behaviour
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Abstracts / Journal of Biotechnology 161S (2012) 19–48
Potential of boron compounds as pesticides
Bioremediation
Omer Edip Kuzugudenli
Researches regarding the effect of thermal power station ashes on the heavy metal content of Festuca arundinacea Schreb. plants
Erciyes University, School of Sciences, Kayseri 38039, Turkey E-mail address: [email protected]. Pesticides are the chemicals used for destruction or control of undesired organisms. This broad definition can cover any chemicals used to fight unwanted insects, fungi and plants. Since the ancient times, numerous types of organic and inorganic chemicals have been applied as pesticides. The use of most inorganic pesticides has been reduced in recent years in favour of organic ones for they cannot degrade and they have the potential to negatively affect human and warm-blooded animals. A new generation of inexpensive and very powerful synthetic organic chemicals has been universally used in ever increasing amounts. Unfortunately, these synthetic pesticides have many shortcomings, too; their slow degradation, build up in the environment and living organisms, and potential to have carcinogen effects, to name a few. In this study, commonly used pesticide types are evaluated, and the potential benefits of applying boron containing pesticides are discussed. Also presented are preliminary experimental results on the use of boric acid as a pesticide on cockroaches. The experimental findings suggest that boric acid can act as an insecticide with long lasting and environmentally friendly effects. http://dx.doi.org/10.1016/j.jbiotec.2012.07.106
Sorin Gaspar ∗ , Florin Marian, Ioan Banatean Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara 300645, Romania E-mail address: [email protected] (S. Gaspar). Ash pits from thermal power stations can be cultivated with plants tolerant to high heavy metal concentrations, but which also produce biomass, thus being used for energy cultures. Festuca arundinacea seeds were sowed in pots with the following substrate variants: 100% soil (control), soil ash 1:1 and 100% ash (from CET Timisoara). The heavy metal content in above-ground parts of plants was determined using atomic absorption spectrophotometer. The concentration of copper in plants cultivated in the substrate mixture was 12.70% higher, meanwhile in 100% ash variant, with 16.2% (54.15 mg/kg) higher compared to control. Cobalt concentration increased with 6.86% on the substrate mixture and with 21.12% (53.86 mg/kg) on the ash substrate. Zinc concentration decreased on variants with ash. In case of iron, concentrations increased with 8.18% in substrate mixture and decreased with 14.27% in ash variant. Absorption was influenced by values of pH. It was observed that the increase of concentrations was not raising toxicity issues. Acknowledgement: Study supported by POSDRU/89/1.5/S/62371.
The treatments effect with pesticides at canola on honeybees behaviour
http://dx.doi.org/10.1016/j.jbiotec.2012.07.108
Alin Jivan, Valeriu Tabara, Adina Horablaga, Antonina Riscu ∗ , Sorin Gaspar, Irina Jurmescu
Food and Agricultural Biosecurity
Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara 300645, Romania E-mail address: [email protected] (A. Riscu). Beekeeping has a major economic importance, because these have an important role in most crops pollination that is destined for seed production. This study is part of the first researches realised on canola culture, in Romania and was observed the evolution of honeybees family, before and after the application of pesticides, which represents a stress factor. After seven days from the treatment application the number of honeybees identified at the entry in the beehive decreased sudden, from a maximum number of 520 honeybees registered at the beehive entrance at the beginning of researches to a minimum number of 285 honeybees, and after 14 days from the pesticides application, the number of identified honeybees started to increase till 495 honeybees. In the reference period was measured also the quantity of brood, which varied between 1320 cm2 and 940 cm2 , being dependent on the used pesticide at canola culture and on the climate conditions from each research area. Acknowledgement: Study supported by POSDRU/89/1.5/S/62371. http://dx.doi.org/10.1016/j.jbiotec.2012.07.107
Genetic diversity of Listeria monocytogenes isolated from retail beef in Erzurum, Turkey Gulsah Adiguzel 1 , Medine Gulluce 2 , Ceyda Bozoglu 3,∗ , Derya Yanmis 2 , Ahmet Adiguzel 3 1 Department of Food Science and Technology, Faculty of Veterinary Science, Ataturk University, Erzurum 25240, Turkey 2 Department of Biology, Faculty of Science, Ataturk University, Erzurum 25240, Turkey 3 Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum 25240, Turkey
E-mail address: [email protected] (C. Bozoglu). The correct identification of food bacterial pathogens is important for the food safety and the monitoring of the food contamination during the food supplementations. The objective of this study was to identify and characterize Listeria monocytogenes strains isolated from meat samples, Erzurum, Turkey, by using rep-PCR fingerprinting technique. By using selective CHROMagarTM Listeria (RTA, Turkey) medium, L. monocytogenes strains were isolated from meat samples. The genomic fingerprinting analyses of test strains were performed by using rep-PCR. After evaluating several primer sets targeting the repetitive DNA elements of REP, ERIC and BOX, the ERIC primers were found to be the most reliable technique. Therefore, rep-PCR fingerprinting using the ERIC can be considered as a promising genotypic tool for the identification and characterization of L. monocytogenes from species to serotype level. http://dx.doi.org/10.1016/j.jbiotec.2012.07.109
Abstracts / Journal of Biotechnology 161S (2012) 19–48
Potential of boron compounds as pesticides
Bioremediation
Omer Edip Kuzugudenli
Researches regarding the effect of thermal power station ashes on the heavy metal content of Festuca arundinacea Schreb. plants
Erciyes University, School of Sciences, Kayseri 38039, Turkey E-mail address: [email protected]. Pesticides are the chemicals used for destruction or control of undesired organisms. This broad definition can cover any chemicals used to fight unwanted insects, fungi and plants. Since the ancient times, numerous types of organic and inorganic chemicals have been applied as pesticides. The use of most inorganic pesticides has been reduced in recent years in favour of organic ones for they cannot degrade and they have the potential to negatively affect human and warm-blooded animals. A new generation of inexpensive and very powerful synthetic organic chemicals has been universally used in ever increasing amounts. Unfortunately, these synthetic pesticides have many shortcomings, too; their slow degradation, build up in the environment and living organisms, and potential to have carcinogen effects, to name a few. In this study, commonly used pesticide types are evaluated, and the potential benefits of applying boron containing pesticides are discussed. Also presented are preliminary experimental results on the use of boric acid as a pesticide on cockroaches. The experimental findings suggest that boric acid can act as an insecticide with long lasting and environmentally friendly effects. http://dx.doi.org/10.1016/j.jbiotec.2012.07.106
Sorin Gaspar ∗ , Florin Marian, Ioan Banatean Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara 300645, Romania E-mail address: [email protected] (S. Gaspar). Ash pits from thermal power stations can be cultivated with plants tolerant to high heavy metal concentrations, but which also produce biomass, thus being used for energy cultures. Festuca arundinacea seeds were sowed in pots with the following substrate variants: 100% soil (control), soil ash 1:1 and 100% ash (from CET Timisoara). The heavy metal content in above-ground parts of plants was determined using atomic absorption spectrophotometer. The concentration of copper in plants cultivated in the substrate mixture was 12.70% higher, meanwhile in 100% ash variant, with 16.2% (54.15 mg/kg) higher compared to control. Cobalt concentration increased with 6.86% on the substrate mixture and with 21.12% (53.86 mg/kg) on the ash substrate. Zinc concentration decreased on variants with ash. In case of iron, concentrations increased with 8.18% in substrate mixture and decreased with 14.27% in ash variant. Absorption was influenced by values of pH. It was observed that the increase of concentrations was not raising toxicity issues. Acknowledgement: Study supported by POSDRU/89/1.5/S/62371.
The treatments effect with pesticides at canola on honeybees behaviour
http://dx.doi.org/10.1016/j.jbiotec.2012.07.108
Alin Jivan, Valeriu Tabara, Adina Horablaga, Antonina Riscu ∗ , Sorin Gaspar, Irina Jurmescu
Food and Agricultural Biosecurity
Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara 300645, Romania E-mail address: [email protected] (A. Riscu). Beekeeping has a major economic importance, because these have an important role in most crops pollination that is destined for seed production. This study is part of the first researches realised on canola culture, in Romania and was observed the evolution of honeybees family, before and after the application of pesticides, which represents a stress factor. After seven days from the treatment application the number of honeybees identified at the entry in the beehive decreased sudden, from a maximum number of 520 honeybees registered at the beehive entrance at the beginning of researches to a minimum number of 285 honeybees, and after 14 days from the pesticides application, the number of identified honeybees started to increase till 495 honeybees. In the reference period was measured also the quantity of brood, which varied between 1320 cm2 and 940 cm2 , being dependent on the used pesticide at canola culture and on the climate conditions from each research area. Acknowledgement: Study supported by POSDRU/89/1.5/S/62371. http://dx.doi.org/10.1016/j.jbiotec.2012.07.107
Genetic diversity of Listeria monocytogenes isolated from retail beef in Erzurum, Turkey Gulsah Adiguzel 1 , Medine Gulluce 2 , Ceyda Bozoglu 3,∗ , Derya Yanmis 2 , Ahmet Adiguzel 3 1 Department of Food Science and Technology, Faculty of Veterinary Science, Ataturk University, Erzurum 25240, Turkey 2 Department of Biology, Faculty of Science, Ataturk University, Erzurum 25240, Turkey 3 Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum 25240, Turkey
E-mail address: [email protected] (C. Bozoglu). The correct identification of food bacterial pathogens is important for the food safety and the monitoring of the food contamination during the food supplementations. The objective of this study was to identify and characterize Listeria monocytogenes strains isolated from meat samples, Erzurum, Turkey, by using rep-PCR fingerprinting technique. By using selective CHROMagarTM Listeria (RTA, Turkey) medium, L. monocytogenes strains were isolated from meat samples. The genomic fingerprinting analyses of test strains were performed by using rep-PCR. After evaluating several primer sets targeting the repetitive DNA elements of REP, ERIC and BOX, the ERIC primers were found to be the most reliable technique. Therefore, rep-PCR fingerprinting using the ERIC can be considered as a promising genotypic tool for the identification and characterization of L. monocytogenes from species to serotype level. http://dx.doi.org/10.1016/j.jbiotec.2012.07.109