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Topical zinc Orabase (5%) in reducing the oral mucositis during cancer chemoradiotherapy
ABSTRACTS
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Multiple sequence alignment revealed amino acid profiles showing conserved independent lineage specific substitutions. Phylogenetic analysis revealed that HPV proteins evolved in a similar manner. Conclusions: The present results provide an overview of the characteristics of HPV proteins and their evolutionary changes, potentially explaining the pathogenesis of oral cancer caused by HPVs.
THE EVALUATION OF SEQUENTIAL TYPE OR DE NOVO TYPE IN 100 CASES OF ORAL SQUAMOUS CELL CARCINOMA. RIE KAWASHIMA, YOSHINORI JINBU, NARUO OKADA, JUNICHI HAYASAKA, TADAHIDE NOGUCHI, YOSHIYUKI MORI. JICHI MEDICAL UNIVERSITY, JAPAN Objectives: Oral potentially malignant disorders (OPMDs) have been suggested as oral precancers, including both oral precancerous regions and oral precancerous conditions. Oral squamous cell carcinoma (OSCC) is divided into 2 types according to the appearances of the cancerous regions. One type is carcinomas with precancerous white lesions—the sequential type. The other type is accompanied SCC without precancerous white lesions—the de novo type. The aim of this study was to evaluate the clinicopathologic characteristics in the sequential type and the de novo type of OSCC. Study Design: We analyzed 100 cases of OSCC of the tongue diagnosed at Jichi Medical University. We divided these OSCC cases into 2 types, using intraoral photographs taken by an oral surgeon. Statistical analysis was done by using the x2 test (P < .05). Results: Of the 100 cases, there were 51 cases of the sequential type (51%) and 49 cases of the de novo type (49%). The percentage of the T1+T2 group (92.2%) in the sequential type was considerably higher than that of T3+T4 group (7.8%). In the de novo type, the T1+T2 group showed 77.6% and the T3+T4 group showed 22.4%. The percentage of lymph node metastasis in the negative group (84.3%) in the sequential type was higher than that of the positive group (15.7%). In the de novo type, the lymph node metastasis negative group showed 65.3% and the positive group showed 34.7%. These were significant differences. With regard to postoperative lymph node metastasis and the YamamotoKohama classification of modes of invasion, there were no significant differences in the sequential type and the de novo type. With regard to pathologic findings, the margins of the cancerous lesions in the sequential type tended to be connected to dysplasia or oral intraepithelial neoplasia. It also showed the unclear margin. However, it showed clear margins in the de novo type. Conclusions: In this study, we found that patients with sequential type tended to seek treatment in T1+T2 (early stages) and to have no lymph node metastasis, compared with the cases of the de novo type. Our results suggested that there was a relationship between the sequential type and OPMDs.
TOPICAL ZINC ORABASE (5%) IN REDUCING THE ORAL MUCOSITIS DURING CANCER CHEMORADIOTHERAPY. SOURAB ABHYANKAR, KAVYA ALLURI, PUNDARIKAKSHAIAH DEVENENI, JAYASURYA KONDAPANENI, NALLAN C.S. K. CHAITANYA, SRI LAXMI MANASA PENMETSA. PANINEEYA MAHAVIDYALAYA INSTITUTE OF DENTAL SCIENCES AND RESEARCH CENTRE, INDIA Objectives: Oral mucositis, a painful sequala of cancer chemoradiotherapy, has a significant effect on the oral mucosa. Several studies have shown that zinc could reduce the severity of
OOOO January 2020 oral mucositis. However, zinc formulations are available only in the form of tablets, which poses difficulty in administration during oral mucositis. The present study aimed to compare and evaluate the efficacy of topical zinc and the improvised zinc Orabase in the treatment of oral mucositis. Study Design: The study was conducted at the MNJ Regional Oncology Centre among 75 patients. It was a single-center, single-blinded, randomized trial, in which 50 patients were case clusters and the remaining 25 patients were controls. The case group was randomly subdivided into 2 groups— group A with 25 patients who received topical oral 5% zinc paste, and group B consisting of 25 patients who received a combination of topical oral 5% zinc paste with the improvised zinc preparation three times daily for 35 days. Twenty-five patients were controls, who were prescribed the standard treatment provided at the cancer hospital. The groups were assessed for mucositis by the World Health Organization grading system on days 7, 14, 21, 28, and 35. Results: An overall comparison among the groups and the degrees of severity of oral mucositis revealed that there was statistically significant difference among all the groups compared with the controls (analysis of variance [ANOVA]), and intergroup variations were also present. On the x2 test, zinc Orabase group performed better on day 35 compared with the controls (0.037; P < .05), the improvised preparation of zinc showed near significance during days 7, 21, and 35 of therapy. The improvised zinc performed better than the zinc alone during days 21 and 35 (0.029, 0.013; P < .05). Conclusions: The severity of oral mucositis decreased in both zinc intervention groups, but the improvised zinc paste Orabase (5%) showed overall decrease in the severity of oral mucositis. Further study with a larger sample size is necessary to establish the validity of this formulation for oncology practice.
ENHANCED PROLIFERATION AND INTERLEUKIN-6 SECRETION OF T CELLS MEDIATED BY OVEREXPRESSION OF TRIM21 IN THE ORAL LESIONS OF PATIENTS WITH ORAL LICHEN PLANUS. YUFENG WANGA,B,C, WEI WEIA,B,C, QIANQIAN SUNA, LEI PANA,B,C, CHENXI LIA,B,C, GUOYAO TANGA,B,C. A DEPARTMENT OF ORAL MEDICINE, NINTH PEOPLE’S HOSPITAL, SHANGHAI JIAOTONG UNIVERSITY SCHOOL OF MEDICINE, CHINA, B SHANGHAI KEY LABORATORY OF STOMATOLOGY & SHANGHAI RESEARCH INSTITUTE OF STOMATOLOGY, CHINA, AND C NATIONAL CLINICAL RESEARCH CENTER OF STOMATOLOGY, CHINA Objectives: The aim of this study was to screen the interacting proteins of TLR9 and to explore the function of TRIM21 overexpression. Study Design: Buccal mucosal samples and peripheral blood mononuclear cells (PBMCs) were collected from both patients with oral lichen planus (OLP) and the healthy control group for the following studies: (1) co-immunoprecipitation (CoIP), followed by mass spectrometry, to screen for the interacting proteins of TLR9 in OLP lesions; (2) immunofluorescence colocalization analysis and plasmid transfection plus CoIP to verify protein interaction; (3) quantitative polymerase chain reaction (qPCR) to quantify its gene expression; immunohistochemistry to delineate its spatial distribution; (4) overexpression of this gene in T cells to test its function. Results: (1) Of the 7 potential TLR9 interacting proteins predominantly present in OLP samples, TRIM21 was selected as
e190
Multiple sequence alignment revealed amino acid profiles showing conserved independent lineage specific substitutions. Phylogenetic analysis revealed that HPV proteins evolved in a similar manner. Conclusions: The present results provide an overview of the characteristics of HPV proteins and their evolutionary changes, potentially explaining the pathogenesis of oral cancer caused by HPVs.
THE EVALUATION OF SEQUENTIAL TYPE OR DE NOVO TYPE IN 100 CASES OF ORAL SQUAMOUS CELL CARCINOMA. RIE KAWASHIMA, YOSHINORI JINBU, NARUO OKADA, JUNICHI HAYASAKA, TADAHIDE NOGUCHI, YOSHIYUKI MORI. JICHI MEDICAL UNIVERSITY, JAPAN Objectives: Oral potentially malignant disorders (OPMDs) have been suggested as oral precancers, including both oral precancerous regions and oral precancerous conditions. Oral squamous cell carcinoma (OSCC) is divided into 2 types according to the appearances of the cancerous regions. One type is carcinomas with precancerous white lesions—the sequential type. The other type is accompanied SCC without precancerous white lesions—the de novo type. The aim of this study was to evaluate the clinicopathologic characteristics in the sequential type and the de novo type of OSCC. Study Design: We analyzed 100 cases of OSCC of the tongue diagnosed at Jichi Medical University. We divided these OSCC cases into 2 types, using intraoral photographs taken by an oral surgeon. Statistical analysis was done by using the x2 test (P < .05). Results: Of the 100 cases, there were 51 cases of the sequential type (51%) and 49 cases of the de novo type (49%). The percentage of the T1+T2 group (92.2%) in the sequential type was considerably higher than that of T3+T4 group (7.8%). In the de novo type, the T1+T2 group showed 77.6% and the T3+T4 group showed 22.4%. The percentage of lymph node metastasis in the negative group (84.3%) in the sequential type was higher than that of the positive group (15.7%). In the de novo type, the lymph node metastasis negative group showed 65.3% and the positive group showed 34.7%. These were significant differences. With regard to postoperative lymph node metastasis and the YamamotoKohama classification of modes of invasion, there were no significant differences in the sequential type and the de novo type. With regard to pathologic findings, the margins of the cancerous lesions in the sequential type tended to be connected to dysplasia or oral intraepithelial neoplasia. It also showed the unclear margin. However, it showed clear margins in the de novo type. Conclusions: In this study, we found that patients with sequential type tended to seek treatment in T1+T2 (early stages) and to have no lymph node metastasis, compared with the cases of the de novo type. Our results suggested that there was a relationship between the sequential type and OPMDs.
TOPICAL ZINC ORABASE (5%) IN REDUCING THE ORAL MUCOSITIS DURING CANCER CHEMORADIOTHERAPY. SOURAB ABHYANKAR, KAVYA ALLURI, PUNDARIKAKSHAIAH DEVENENI, JAYASURYA KONDAPANENI, NALLAN C.S. K. CHAITANYA, SRI LAXMI MANASA PENMETSA. PANINEEYA MAHAVIDYALAYA INSTITUTE OF DENTAL SCIENCES AND RESEARCH CENTRE, INDIA Objectives: Oral mucositis, a painful sequala of cancer chemoradiotherapy, has a significant effect on the oral mucosa. Several studies have shown that zinc could reduce the severity of
OOOO January 2020 oral mucositis. However, zinc formulations are available only in the form of tablets, which poses difficulty in administration during oral mucositis. The present study aimed to compare and evaluate the efficacy of topical zinc and the improvised zinc Orabase in the treatment of oral mucositis. Study Design: The study was conducted at the MNJ Regional Oncology Centre among 75 patients. It was a single-center, single-blinded, randomized trial, in which 50 patients were case clusters and the remaining 25 patients were controls. The case group was randomly subdivided into 2 groups— group A with 25 patients who received topical oral 5% zinc paste, and group B consisting of 25 patients who received a combination of topical oral 5% zinc paste with the improvised zinc preparation three times daily for 35 days. Twenty-five patients were controls, who were prescribed the standard treatment provided at the cancer hospital. The groups were assessed for mucositis by the World Health Organization grading system on days 7, 14, 21, 28, and 35. Results: An overall comparison among the groups and the degrees of severity of oral mucositis revealed that there was statistically significant difference among all the groups compared with the controls (analysis of variance [ANOVA]), and intergroup variations were also present. On the x2 test, zinc Orabase group performed better on day 35 compared with the controls (0.037; P < .05), the improvised preparation of zinc showed near significance during days 7, 21, and 35 of therapy. The improvised zinc performed better than the zinc alone during days 21 and 35 (0.029, 0.013; P < .05). Conclusions: The severity of oral mucositis decreased in both zinc intervention groups, but the improvised zinc paste Orabase (5%) showed overall decrease in the severity of oral mucositis. Further study with a larger sample size is necessary to establish the validity of this formulation for oncology practice.
ENHANCED PROLIFERATION AND INTERLEUKIN-6 SECRETION OF T CELLS MEDIATED BY OVEREXPRESSION OF TRIM21 IN THE ORAL LESIONS OF PATIENTS WITH ORAL LICHEN PLANUS. YUFENG WANGA,B,C, WEI WEIA,B,C, QIANQIAN SUNA, LEI PANA,B,C, CHENXI LIA,B,C, GUOYAO TANGA,B,C. A DEPARTMENT OF ORAL MEDICINE, NINTH PEOPLE’S HOSPITAL, SHANGHAI JIAOTONG UNIVERSITY SCHOOL OF MEDICINE, CHINA, B SHANGHAI KEY LABORATORY OF STOMATOLOGY & SHANGHAI RESEARCH INSTITUTE OF STOMATOLOGY, CHINA, AND C NATIONAL CLINICAL RESEARCH CENTER OF STOMATOLOGY, CHINA Objectives: The aim of this study was to screen the interacting proteins of TLR9 and to explore the function of TRIM21 overexpression. Study Design: Buccal mucosal samples and peripheral blood mononuclear cells (PBMCs) were collected from both patients with oral lichen planus (OLP) and the healthy control group for the following studies: (1) co-immunoprecipitation (CoIP), followed by mass spectrometry, to screen for the interacting proteins of TLR9 in OLP lesions; (2) immunofluorescence colocalization analysis and plasmid transfection plus CoIP to verify protein interaction; (3) quantitative polymerase chain reaction (qPCR) to quantify its gene expression; immunohistochemistry to delineate its spatial distribution; (4) overexpression of this gene in T cells to test its function. Results: (1) Of the 7 potential TLR9 interacting proteins predominantly present in OLP samples, TRIM21 was selected as