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Topographic arrangement of the projection from the anterior thalamic nuclei to the cingulate cortex in the cat
62
Neuroscience Research, 4 (1986) 62-66 Elsevier Scientific Publishers Ireland Ltd.
NSR 00151
Topographic arrangement of the projection from the anterior thalamic nuclei to the cingulate cortex in the cat Hiroaki Matsuoka Department of Anatono,, Okaj,ama UniversityMedical School Okayama (JapalO
(Received 5 March 1986; Accepted 15 May 1986) KO' words: Anterior thalamic nucleus; Cingulate cortex; cat; HRP
SUMMARY After injection of H RP into the cingulate and its adjacent cortical areas, neuronal cells were retrogradely labeled ipsilatera[ly in the anterior, lateral, ventral, intralaminar and midline thalamic nuclei. Most of them occurred in the anterior nuclei, and a topographic correlation was revealed in the projections from the anterior thalamic nuclei to the cingulate gyrus: the rostral part of the cingulatc gyrus receives fibers predominantly from the lateral part of the AM and additionally from the caudomedial part of the AV. The caudal part of the cingulatc gyrus receives fibers from the medial part of the anteromcdial nucleus (AM) and the rostromcdial part of the anteroventral nucleus (AV). The intermediate part of the cingulate gyrus receives fibers from the intermediate part of the AM and the caudal half of the AV. O n the basis o f the retrograde degeneration study in the cat and rabbit, Rose and Woolsey 6 concluded that the anteromedial nucleus (AM) sends fibers ipsilatcraliy to the anterior limbic area (La), the anteroventral nucleus (AV) to the cingulate (Cg), and the anterodorsal nucleus (AD) to the retrosplenial area (Rs). Later anatomical studies, however, have indicated that both A M and AV project to the Cg, Rs and presubiculum ~'5, that the projection fields from the A M and AV to the Cg somewhat overlap each other 2, and that the thalamic projections to the anterior part o f t h e Cg differ from those to the posterior part of the Cg z.7. Thus the present experiments have been carried out in order to shed more light on the thalamocingulate projections by the H R P method. Eight adult cats were used in this study. A small amount (0. l-0.14/11) ofhorseradish peroxidase ( H R P , Sigma Type VI or T o y o b o G r a d e I-C) was injected (Fig. IA) Correspondence: H. Matsuoka, Department of Anatomy, Okayama University Medical School, Okayama 700, Japan. 0168-0102/86/$03.50 9 1986 Elsevier Scientific Publishers Ireland Ltd.
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Fig. 1. A: an injection site of HRP in the cingulate cortex of'cat 412L A frontal section. An arrow indicates the splcnial sulcus. Bar = 3 ram. B: photomicrograph of a group &labeled cc]ls from the middle part of the AM in cat 494L Bar = 200/~rn.
unilaterally or bilaterally into 12 different parts of the Cg and adjacent areas in the cat (Fig. 3). Other technical details were as described previously4. Results of 4 experiments where HRP was injected into different parts of the eingulate gyrus are shown in Fig. 2. In each experiment, many labeled cells were found in the anterior, lateral and midline thalamic nuclei, and a smaller number of them were found in the ventroanterior (VA), ventrolateral (VL) and intralaminar thalamic nuclei. The greatest number of labeled cells occurred in the AM and AV; the distribution pattern of the labeled neuronal cell bodies was varied among the 4 experiments. When HRP was injected into a region in the most rostral part ofthe Cg (cat 494R), a group oflabeled cells was found in the ventrolateral part of the AM, and only a few labeled cells were seen in the caudomedial part of the AV (not illustrated in the drawing). In cats 515L and 495L (not illustrated in Fig. 2), where HRP was injected into the Cg at the level of the splenium corporis callosi, a band of labeled cells was found in the medial part of both AM and AV. When HRP was injected in a region of the most caudal part of the Cg (cat 412L, Fig. IA and Fig. 3), labeled cells appeared in the rostromedial part ofthe AM and AV. These cells were located more rostrally than those seen in cat 515L. After injection of HRP into the middle part of the Cg (cat 494L), a laminar band of labeled cells occurred in the intermediate part of the AM ( Fig IB). In the AV, most of
64
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Fig. 2. Distribution oflabeled cells (indicated by dots) in the thalamic nuclei (below) after injection of Ii RP into the cingul:ltc gyrns (above). Injections in lhc left cingulalc g~rus in 2 experiments (marked L) have been lransferred to the right side (R).
the labeled cells occurred in the caudal half of the nucleus. In cats 515R and 495R (not illustrated in Fig. 2), where the injectinn sites of the enzyme were located more caudally than in cat 494L, most labeled cells were seen in the intermediate part of the AM and in the ventromedial part of the AV.
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Fig. 3. The topographic projection from the anterior thalamic nuclei to the cingulate gyrus. Same symbok are used for cells of origin and termination of the projection.
In all experiments except in cat 494R, a few labeled neuronal cell bodies occurred in the AD. In 5 other experiments (cats 40L, 184R, 488R, 554R and L, Fig. 3) where HRP was injected into the anterior limbic region (La) and the precentral agranular area (Prag), retrogradely labeled cells were found in the AM, but not in the AV. The labeled cells occurred only in the most caudal part of the AM in cats 488R, 554R and L. In the present study, a topographic organization was revealed in the projections from the AM and AV to the Cg; the caudal part of the Cg receives afferent fibers from the rostromedial parts of the AM and the AV, while the rostrai part of the Cg receives projection fibers from the caudolateral part of the AM and the most caudal part of the AV.
66 ABBREVIATIONS
AD AM AV CA CC CD Cg CM II La LD MD Of
anterodorsal nucleus anteromedial nucleus anteroventral nucleus centroanterior nucleus corpus callosum centrodorsal nucleus cingular area centromedial nucleus infralimbic area anterior limbic region laterodorsal nucleus mediodorsal nucleus orbitofrontal region
PC Prag Ps Rs Re Rh Rt Sb SM VA VL VM VPL
paracentral nucleus precentral agranular area postsubicular area retrosplenial area reuniens nucleus rhomboid nucleus reticular nucleus submedius nucleus stria medullaris ventroanterior nucleus ventrolateral nucleus ventromedial nucleus posterolateral ventral nucleus
REFERENCES l Fujii, M., Fiber cannections between the thalamic posterior lateral nuc!eus and the cingulate gyrus in the eat, NeuroscL Lett., 39 (1983) 137-142. 2 Niimi, M., Cortical projections of the anterior thalamic nuclei in the cat, Esp. Brain Res., 31 (1978) 403-416. 3 Niimi, K., Niimi, M. and Okada, Y., Thalamic afferents to the limbie cortex in the cat studied with the method of retrograde axonal transport of horseradish peroxidase, Brain Res., 145 (1978) 225-238. 4 Niimi, K., Niimi, M., Matsuoka, H., Yanagihara, M. and Katayama, T., The laminar arrangement of limbie th~lamoeortical neurons in the lateropulvinar nuclei ofthe cat thalamus,Neurosci. Lett., 38 (1983) 215-219. 5 Robertson, R.T. and Kaitz, S.S., Thalamic connections with limbic cortex. 1. Thalamocortical projections, J. Cutup. NeuroL, 195 (1981) 501-525. 6 Rose, J.E. and Woolsey, C.N., Structure and relations oflimbic cortex and anterior thalamie nuclei in rabbi~ and cat, d. Cutup. Neurol. 89 (1948) 279-348. 7 V~g~, B.A., Rosene, D.L. and Pandya, D.N., Thalamie and cortical afferents differentiate anterior from po.;~c~Jor cingulate cortex in monkey, Science, 204 (1979) 205-207.
Neuroscience Research, 4 (1986) 62-66 Elsevier Scientific Publishers Ireland Ltd.
NSR 00151
Topographic arrangement of the projection from the anterior thalamic nuclei to the cingulate cortex in the cat Hiroaki Matsuoka Department of Anatono,, Okaj,ama UniversityMedical School Okayama (JapalO
(Received 5 March 1986; Accepted 15 May 1986) KO' words: Anterior thalamic nucleus; Cingulate cortex; cat; HRP
SUMMARY After injection of H RP into the cingulate and its adjacent cortical areas, neuronal cells were retrogradely labeled ipsilatera[ly in the anterior, lateral, ventral, intralaminar and midline thalamic nuclei. Most of them occurred in the anterior nuclei, and a topographic correlation was revealed in the projections from the anterior thalamic nuclei to the cingulate gyrus: the rostral part of the cingulatc gyrus receives fibers predominantly from the lateral part of the AM and additionally from the caudomedial part of the AV. The caudal part of the cingulatc gyrus receives fibers from the medial part of the anteromcdial nucleus (AM) and the rostromcdial part of the anteroventral nucleus (AV). The intermediate part of the cingulate gyrus receives fibers from the intermediate part of the AM and the caudal half of the AV. O n the basis o f the retrograde degeneration study in the cat and rabbit, Rose and Woolsey 6 concluded that the anteromedial nucleus (AM) sends fibers ipsilatcraliy to the anterior limbic area (La), the anteroventral nucleus (AV) to the cingulate (Cg), and the anterodorsal nucleus (AD) to the retrosplenial area (Rs). Later anatomical studies, however, have indicated that both A M and AV project to the Cg, Rs and presubiculum ~'5, that the projection fields from the A M and AV to the Cg somewhat overlap each other 2, and that the thalamic projections to the anterior part o f t h e Cg differ from those to the posterior part of the Cg z.7. Thus the present experiments have been carried out in order to shed more light on the thalamocingulate projections by the H R P method. Eight adult cats were used in this study. A small amount (0. l-0.14/11) ofhorseradish peroxidase ( H R P , Sigma Type VI or T o y o b o G r a d e I-C) was injected (Fig. IA) Correspondence: H. Matsuoka, Department of Anatomy, Okayama University Medical School, Okayama 700, Japan. 0168-0102/86/$03.50 9 1986 Elsevier Scientific Publishers Ireland Ltd.
63
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Fig. 1. A: an injection site of HRP in the cingulate cortex of'cat 412L A frontal section. An arrow indicates the splcnial sulcus. Bar = 3 ram. B: photomicrograph of a group &labeled cc]ls from the middle part of the AM in cat 494L Bar = 200/~rn.
unilaterally or bilaterally into 12 different parts of the Cg and adjacent areas in the cat (Fig. 3). Other technical details were as described previously4. Results of 4 experiments where HRP was injected into different parts of the eingulate gyrus are shown in Fig. 2. In each experiment, many labeled cells were found in the anterior, lateral and midline thalamic nuclei, and a smaller number of them were found in the ventroanterior (VA), ventrolateral (VL) and intralaminar thalamic nuclei. The greatest number of labeled cells occurred in the AM and AV; the distribution pattern of the labeled neuronal cell bodies was varied among the 4 experiments. When HRP was injected into a region in the most rostral part ofthe Cg (cat 494R), a group oflabeled cells was found in the ventrolateral part of the AM, and only a few labeled cells were seen in the caudomedial part of the AV (not illustrated in the drawing). In cats 515L and 495L (not illustrated in Fig. 2), where HRP was injected into the Cg at the level of the splenium corporis callosi, a band of labeled cells was found in the medial part of both AM and AV. When HRP was injected in a region of the most caudal part of the Cg (cat 412L, Fig. IA and Fig. 3), labeled cells appeared in the rostromedial part ofthe AM and AV. These cells were located more rostrally than those seen in cat 515L. After injection of HRP into the middle part of the Cg (cat 494L), a laminar band of labeled cells occurred in the intermediate part of the AM ( Fig IB). In the AV, most of
64
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the labeled cells occurred in the caudal half of the nucleus. In cats 515R and 495R (not illustrated in Fig. 2), where the injectinn sites of the enzyme were located more caudally than in cat 494L, most labeled cells were seen in the intermediate part of the AM and in the ventromedial part of the AV.
65
1 /
2
3
4
Fig. 3. The topographic projection from the anterior thalamic nuclei to the cingulate gyrus. Same symbok are used for cells of origin and termination of the projection.
In all experiments except in cat 494R, a few labeled neuronal cell bodies occurred in the AD. In 5 other experiments (cats 40L, 184R, 488R, 554R and L, Fig. 3) where HRP was injected into the anterior limbic region (La) and the precentral agranular area (Prag), retrogradely labeled cells were found in the AM, but not in the AV. The labeled cells occurred only in the most caudal part of the AM in cats 488R, 554R and L. In the present study, a topographic organization was revealed in the projections from the AM and AV to the Cg; the caudal part of the Cg receives afferent fibers from the rostromedial parts of the AM and the AV, while the rostrai part of the Cg receives projection fibers from the caudolateral part of the AM and the most caudal part of the AV.
66 ABBREVIATIONS
AD AM AV CA CC CD Cg CM II La LD MD Of
anterodorsal nucleus anteromedial nucleus anteroventral nucleus centroanterior nucleus corpus callosum centrodorsal nucleus cingular area centromedial nucleus infralimbic area anterior limbic region laterodorsal nucleus mediodorsal nucleus orbitofrontal region
PC Prag Ps Rs Re Rh Rt Sb SM VA VL VM VPL
paracentral nucleus precentral agranular area postsubicular area retrosplenial area reuniens nucleus rhomboid nucleus reticular nucleus submedius nucleus stria medullaris ventroanterior nucleus ventrolateral nucleus ventromedial nucleus posterolateral ventral nucleus
REFERENCES l Fujii, M., Fiber cannections between the thalamic posterior lateral nuc!eus and the cingulate gyrus in the eat, NeuroscL Lett., 39 (1983) 137-142. 2 Niimi, M., Cortical projections of the anterior thalamic nuclei in the cat, Esp. Brain Res., 31 (1978) 403-416. 3 Niimi, K., Niimi, M. and Okada, Y., Thalamic afferents to the limbie cortex in the cat studied with the method of retrograde axonal transport of horseradish peroxidase, Brain Res., 145 (1978) 225-238. 4 Niimi, K., Niimi, M., Matsuoka, H., Yanagihara, M. and Katayama, T., The laminar arrangement of limbie th~lamoeortical neurons in the lateropulvinar nuclei ofthe cat thalamus,Neurosci. Lett., 38 (1983) 215-219. 5 Robertson, R.T. and Kaitz, S.S., Thalamic connections with limbic cortex. 1. Thalamocortical projections, J. Cutup. NeuroL, 195 (1981) 501-525. 6 Rose, J.E. and Woolsey, C.N., Structure and relations oflimbic cortex and anterior thalamie nuclei in rabbi~ and cat, d. Cutup. Neurol. 89 (1948) 279-348. 7 V~g~, B.A., Rosene, D.L. and Pandya, D.N., Thalamie and cortical afferents differentiate anterior from po.;~c~Jor cingulate cortex in monkey, Science, 204 (1979) 205-207.