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Total protein in leaves of orange (Citrus sinensis), onion (Allium cepa), garlic (Allium sativum) and sweet lemon (Citrus lemon)
Clinical Biochemistry 44 (2011) S225–S268
Contents lists available at ScienceDirect
Clinical Biochemistry j o u r n a l h o m e p a g e : w w w. e l s ev i e r. c o m / l o c a t e / c l i n b i o c h e m
12th ICB and 4th ICBMB (Abstracts of the 12th Iranian Congress of Biochemistry and 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Iran, September 6-9, 2011) Nutritional Biochemistry Poster – [A-10-11-1] Total protein in leaves of orange (Citrus sinensis), onion (Allium cepa), garlic (Allium sativum) and sweet lemon (Citrus lemon) Kosar Aberumand, Mohammad Aberumand Department of Biochemistry, Ahwaz Jondihapour University of Medical Sciences, Ahvaz, Iran E-mail address: [email protected] (K. Aberumand) The objective of present study was to analyze the nutritional composition of four selected plants including: orange (Citrus sinensis), onion (Allium cepa), garlic (Allium sativum) and sweet lemon (Citrus lemon). The results showed that garlic (A. sativum) and onion (A. cepa) had the highest protein (319 mg/100 g and 232.5 mg/100 g, respectively.
Keywords: Fruit, Leaves and total proteins
Results: The results showed that out of 70 cheese samples of traditional cheese 24.28% of them were contaminated with staphylococcus and 2.8% were contaminated with S. aureus. Statistical analysis indicated that distribution of S. aureus per gram of samples was different although the industrial cheeses were not infected with S. aureus as tested with these methods. Conclusion: In order to improve the sanitary quality of cheese and reduce the microbial contamination it is recommended to use pasteurized milk for cheese preparation and perform biochemical and molecular control tests for confirmation of quality control tests. Also, increasing of producers' knowledge about microbial contamination and control tests could be useful for the reduction of contamination during and after cheeses production.
Keywords: Staphylococcus aureus, Traditional cheese, Microbial infection, Detection, Isolation doi:10.1016/j.clinbiochem.2011.08.540
doi:10.1016/j.clinbiochem.2011.08.539
Poster – [A-10-15-1] Detection and isolation of Staphylococcus aureus from produced industrial and traditional cheeses of Mashhad Township using biochemical and molecular tests Hojat Taghipour Razi Vaccine and Serum Research Mashhad Institute, Br, Faculty of Sciences, Piam Noor University Mashhad Center, Mashhad, Iran E-mail address: [email protected]
Poster – [A-10-147-2] The effect of vitamin A on immunophenotyping of peripheral blood lymphocytes by flow cytometry in ulcerative colitis Saeed Abedy Manesha, Abdorrahim Nikzamira, Mohammad Aaberumanda, Alireza Ostadrahimib, Nasim Abedy Maneshb a Biochemistry Department, Ahwaz University of Medical Sciences, Ahwaz, Iran b Health and Nutrition Faculty, Tabriz University of Medical Science, Tabriz, Iran E-mail address: [email protected] (S. Abedy Manesh)
Introduction: Cheese as a source of protein for human, has a place in the diets of people all over the world. Occasionally due to lack of hygienic measures this food may lose some of its nutritional values as an important protein source and can from this way brings about some zoonoses by transmitting pathogens, leading to serious economic losses. The aim of this study was determination of microbial infection of traditional and industrial cheese of Mashhad Township with Staphylococcus aureus by biochemical and molecular methods. Materials and method: With respect to number and place of cheese producers in Mashhad, 10 regions were selected and from each region 10 samples were collected. Finally 70 traditional cheeses and 100 pasteurized cheeses were collected in appropriate conditions. The samples were transferred to the laboratory under sterile condition, and they were grown on specific and selected media. The positive samples were analyzed by biochemical and molecular tests for the presence of S. aureus.
Background: Ulcerative colitis (UC) is an inflammatory disease of the rectal and colonic mucosa and seems to result from a complex series of interactions between susceptibility genes, the environment and the immune system. There is a paucity of data on the positive effect of vitamin A on intestinal mucosal immunity. We aimed to evaluate the effect of vitamin A supplementation on peripheral lymphocyte subset alterations in ulcerative colitis. Methods: Flow cytometric analysis was done in 43 patients with ulcerative colitis participating in pre and post test survey. All participants were at clinical remission stages. Vitamin A supplement was injected twice (50,000 IU) with two weeks interval. Study period was considered for 45 days. Flow cytometric analysis of CD4+ and CD8+ and CD3+ T lymphocytes was done before and after intervention. Results: Vitamin A supplementation had significant effect on decrease of CD8+ T-cells (p= 0.003) while absolute lymphocyte count
0009-9120/$ – see front matter.
Contents lists available at ScienceDirect
Clinical Biochemistry j o u r n a l h o m e p a g e : w w w. e l s ev i e r. c o m / l o c a t e / c l i n b i o c h e m
12th ICB and 4th ICBMB (Abstracts of the 12th Iranian Congress of Biochemistry and 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Iran, September 6-9, 2011) Nutritional Biochemistry Poster – [A-10-11-1] Total protein in leaves of orange (Citrus sinensis), onion (Allium cepa), garlic (Allium sativum) and sweet lemon (Citrus lemon) Kosar Aberumand, Mohammad Aberumand Department of Biochemistry, Ahwaz Jondihapour University of Medical Sciences, Ahvaz, Iran E-mail address: [email protected] (K. Aberumand) The objective of present study was to analyze the nutritional composition of four selected plants including: orange (Citrus sinensis), onion (Allium cepa), garlic (Allium sativum) and sweet lemon (Citrus lemon). The results showed that garlic (A. sativum) and onion (A. cepa) had the highest protein (319 mg/100 g and 232.5 mg/100 g, respectively.
Keywords: Fruit, Leaves and total proteins
Results: The results showed that out of 70 cheese samples of traditional cheese 24.28% of them were contaminated with staphylococcus and 2.8% were contaminated with S. aureus. Statistical analysis indicated that distribution of S. aureus per gram of samples was different although the industrial cheeses were not infected with S. aureus as tested with these methods. Conclusion: In order to improve the sanitary quality of cheese and reduce the microbial contamination it is recommended to use pasteurized milk for cheese preparation and perform biochemical and molecular control tests for confirmation of quality control tests. Also, increasing of producers' knowledge about microbial contamination and control tests could be useful for the reduction of contamination during and after cheeses production.
Keywords: Staphylococcus aureus, Traditional cheese, Microbial infection, Detection, Isolation doi:10.1016/j.clinbiochem.2011.08.540
doi:10.1016/j.clinbiochem.2011.08.539
Poster – [A-10-15-1] Detection and isolation of Staphylococcus aureus from produced industrial and traditional cheeses of Mashhad Township using biochemical and molecular tests Hojat Taghipour Razi Vaccine and Serum Research Mashhad Institute, Br, Faculty of Sciences, Piam Noor University Mashhad Center, Mashhad, Iran E-mail address: [email protected]
Poster – [A-10-147-2] The effect of vitamin A on immunophenotyping of peripheral blood lymphocytes by flow cytometry in ulcerative colitis Saeed Abedy Manesha, Abdorrahim Nikzamira, Mohammad Aaberumanda, Alireza Ostadrahimib, Nasim Abedy Maneshb a Biochemistry Department, Ahwaz University of Medical Sciences, Ahwaz, Iran b Health and Nutrition Faculty, Tabriz University of Medical Science, Tabriz, Iran E-mail address: [email protected] (S. Abedy Manesh)
Introduction: Cheese as a source of protein for human, has a place in the diets of people all over the world. Occasionally due to lack of hygienic measures this food may lose some of its nutritional values as an important protein source and can from this way brings about some zoonoses by transmitting pathogens, leading to serious economic losses. The aim of this study was determination of microbial infection of traditional and industrial cheese of Mashhad Township with Staphylococcus aureus by biochemical and molecular methods. Materials and method: With respect to number and place of cheese producers in Mashhad, 10 regions were selected and from each region 10 samples were collected. Finally 70 traditional cheeses and 100 pasteurized cheeses were collected in appropriate conditions. The samples were transferred to the laboratory under sterile condition, and they were grown on specific and selected media. The positive samples were analyzed by biochemical and molecular tests for the presence of S. aureus.
Background: Ulcerative colitis (UC) is an inflammatory disease of the rectal and colonic mucosa and seems to result from a complex series of interactions between susceptibility genes, the environment and the immune system. There is a paucity of data on the positive effect of vitamin A on intestinal mucosal immunity. We aimed to evaluate the effect of vitamin A supplementation on peripheral lymphocyte subset alterations in ulcerative colitis. Methods: Flow cytometric analysis was done in 43 patients with ulcerative colitis participating in pre and post test survey. All participants were at clinical remission stages. Vitamin A supplement was injected twice (50,000 IU) with two weeks interval. Study period was considered for 45 days. Flow cytometric analysis of CD4+ and CD8+ and CD3+ T lymphocytes was done before and after intervention. Results: Vitamin A supplementation had significant effect on decrease of CD8+ T-cells (p= 0.003) while absolute lymphocyte count
0009-9120/$ – see front matter.