Transfer of blastocysts vitrified using the microsecure vitrification method yields ongoing implantation and pregnancy rates comparable to transfer of fresh embryos

cells in control mice, yet endometrium in hCG administrated mice were remained undifferentiated. (Exp.2) The pregnancy rates of hCG administrated mice and control mice were 66.7% and 100%, respectively. The number of live birth pups per transferred embryos in hCG administrated mice (30.6%) was significantly lower than that of control mice (47.3%). CONCLUSION: Our results indicate that hCG administration for oocyte maturation is the potential cause of the dysfunction of endometrial differentiation and embryonic development during pregnancy in mice.

P-248 Tuesday, October 23, 2012 WITHDRAWN

OBJECTIVE: Array CGH-PGS technology offers rapid, complete chromosomal analysis for aneuploidy screening and sex determination. Interlaboratory next day results from Day 5 BL biopsy accommodates a fresh ET by noon on Day 6. The objective of this study was to assess whether the delayed ET was suboptimal to pregnancy outcome and what factors may be involved. DESIGN: BL-PGS cases (n¼76) were compared by fresh D6 ET (autologous (A), n¼53; donor egg/surrogate(DE/S), n¼10) and PGS-BL vitrification (VTF, n¼13) and contrast to non-PGS fresh D5 BLET cycles (A, n¼188; DE/S, n¼46), and vitrified BL-FET (n¼216) cycles. Differences were determined by Chi-square analysis (P<0.05). MATERIALS AND METHODS: Embryos were cultured in Life Global medium + 7.5% SS (Irvine Sci.) under OvoilÔ (Vitrolife) in triple gas mix at 37 C. Embryos were biopsied following Day 3 laser zona ablation and BL herniation of Day 5 or 6 and then cultured individually. Fresh PGSBLETs were performed on Day 6. All residual biopsied BLs were vitrified by the mircoSecure (mS-VTF) method, excluding ‘‘abnormal’’ Day 5 BLs. RESULTS: PGS patients (mean 37 yo) had significantly lower ongoing pregnancy rates/implantation rates (OPR/IR, respectively: 42%/37%) after D6 ET compared to D6 PGS-DE/S cycles (89%/71%) or PGS-VTFBL (85%/75%). Patients%37 yo seem to be more adversely affected by the delayed BLET (n¼22; 18%/21%) compared to fresh D5 BLET (n¼149, 60%/ 52%) or BL-VTF (n¼113, 60%/44%). Interestingly, women 38-40yo appear less affected by PGS-D6-BLET as indicated by OPR/IR (n¼16, 54%/52%) being similar to BL-VTF cycles (n¼96, 57%/42%) but higher than fresh D5-BLET (n¼49, 47%/32%). CONCLUSION: Effective BL-PGS is negatively impacted by delayed ET into COH-uteri for women%37 yo, yet is beneficial in non-COH uteri cycles. The 38-40 yo patients seem to benefit from BL-PGS for fresh ET, whereas in all cases mS-VTF of BLs has proven to be a highly effective alternative that should be seriously considered in applying PGS technology.

P-250 Tuesday, October 23, 2012 TRANSFER OF BLASTOCYSTS VITRIFIED USING THE MICROSECURE VITRIFICATION METHOD YIELDS ONGOING IMPLANTATION AND PREGNANCY RATES COMPARABLE TO TRANSFER OF FRESH EMBRYOS. H. Dinnie, C. Dowling, P. St. Marie, K. Lynch, M. Arny. Obstetrics and Gynecology, Baystate Medical Center, Springfield, MA.

P-249 Tuesday, October 23, 2012 CONTROLLED OVARIAN HYPERSTIMULATION (COH) CYCLES MAY BENEFIT FROM VITRIFICATION OF ALL DAY 5 PGS-BIOPSIED BLASTOCYSTS. J. Whitney, N. Nugent, K. Duggan, S. Zozula, R. E. Anderson, M. C. Schiewe. Southern California Institute for Reproductive Science, Newport Beach, CA.

OBJECTIVE: To compare the ongoing implantation rates (IR) and pregnancy rates (PR) of vitrified microSecure blastocysts and fresh embryos. DESIGN: A retrospective study of fresh and frozen autologous cycles of patients<41 years old performed between August 15, 2010 and February 30, 2012. All fresh cycles were first cycles that had blastocysts vitrified by the microSecure method. All frozen cycles were second cycles performed when the first fresh cycle did not result in an ongoing pregnancy. MATERIALS AND METHODS: Fresh embryos were cultured in Sage media in 5% O2. Fresh transfers were performed on Day 3 or Day 5. Blastocysts were vitrified and warmed using S3/I.C.E. media and the closed microSecure vitrification method (Schiewe). RESULTS: No statistical difference in the ongoing implantation or pregnancy rates between fresh and frozen cycles was seen. CONCLUSION: MicroSecure vitrified blastocysts produce ongoing implantation and pregnancy rates comparable to those of fresh embryos.

Comparison of Ongoing Implantation and Pregnancy Rates

Ongoing IR (95% CI) Ongoing PR (95% CI) Average Number Embryos Transferred

First Fresh Transfers: Day 3

First Fresh Transfers: Day 5

Frozen Transfers Post Failed First Fresh Transfers

n¼57

n¼78

n¼45

40.4% (28.0%, 52.7%) 50.9% (37.5%, 64.3%) 1.8

48.7% (37.4%, 60.1%) 51.9% (41.2%, 63.9%) 1.3

42.8% (29.4%, 56.2%) 53.3% (38.2%, 68.5%) 1.9

No statistical difference in the ongoing implantation or pregnancy rates between fresh and frozen cycles was seen.

FERTILITY & STERILITYÒ

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