Tritium labeling of peptides and proteins: A critical review

Tritium technology in fission, fusion and isotopic applications SESSION IV:

I.

585

RESEARCH AND DEVELOPMENT

ULTRAVIOLET RADIATION-DAMAGE ABSORPTION PEAK IN SOLID DEUTERIUM-TRITIUM, E. M. Fearon, R. T. Tsugawa, P. C. Souers (Lawrence Livermore National Laboratory), J. D. Poll and J. L. Hunt (Univ. of Guelph Campus)

An ultraviolet absorption peak has been seen in solid deuterium-tritium and hydrogen-trltlum at a sensor temperature of 5 K. and is about 1.5 eV wide.

The peak occurs at 3.6 eV

It bleaches out when the temperature is raised to

about i0 K but reappears upon cooling and is, therefore, radiation induced. At 5 K, the peak forms on a time scale of minutes and appears to represent part-per-million levels of electron-mass defects.

The suggested model is that

of a trapped electron, where the peak is the ground state-to-the-conductlon band transition.

2.

A marked isotope effect is seen between D-T and H-T.

REACTION RATES FOR THE FORMATION OF DEUTERIUM TRITIDE FROM DEUTERIUM AND TRITIUM, G. T. McConville, D. A. Menke and R. E. Ellefson (MRC-Mound)

The rates of formation of DT in a mixture of D 2 and T 2 have been measured as a function

of

initial

T2

concentration,

pressure,

temperature,

and

methane

concentration in a stainless steel reaction container which had been treated to

inhibit

protium

ingrowth.

An

attempt

has

been

made

to

explain

experimental results on the basis of ion-molecule chain reactions.

the

Some of

the observations are consistent with a gas-phase ion, ground-state molecule reaction

but

some

complicated models. catalytic effects

of

the

more

interesting

observations

require

more

The addition of excited state molecules or heterogeneous are possibilities

that will need

further experiments

for

confirmation. 3.

RESEARCH AND DEVELOPMENT IN TRITIUM TECHNOLOGY AT THE INSTITUTE OF RADIOCHEMISTRY, NUCLEAR RESEARCH CENTER KARLSRUHE, H. J. Ache (Kernforschungszentrum, Karlsruhe)

The current and future research and development program in tritium technology at the Institute of Radiochemlstry, Nuclear Research Center Karlsruhe will be reviewed.

The four major objectives of this program will be discussed.

include:

tritium

recovery

from

waste

streams;

development

of

These tritium

decontamination systems for the removal of gaseous trltiated compounds from the

lab

and

glove-box

atmosphere;

impurity

monitoring

in

liquid

metal

blankets; and physiochemical properties of solid breeder materials.

4.

TRITIUM LABELING OF PEPTIDES AND PROTEINS:

A CRITICAL REVIEW,

C. T. Peng, R. L. Hua (Univ. of California San Francisco)

Methods for tritium labeling of peptides and proteins by chemical synthesis, catalytic H-T exchange and radiation-lnduced model

for

the

labeling

of

peptides

activation of tritium gas is proposed.

and

labeling are discussed,

proteins

by

microwave

and a

discharge