- Email: [email protected]
Tu-P10:449 Erythrocyte sedimentation rate and coronary atherosclerosis
Tues&ty, June 20, 2006: Poster Session PIO Biomarkers for ectrdiovascular disectse 5.0 mg/L versus 2.6 mg/L, p<0.05). The lipid profile showed significantly increased values for triglycerides and LDL-cholesterol and decreased concentrations of HDL-cholesterol in the group of patients with stenosis versus control group (p<0.05). The positive relationship between CRP and index of atherosclerosis was observed in the group of cerebrovascular stenosis (r=0.21 ; p < 0.05). Conclusions: CRP measured on automated analysers using high sensitivity assay may be a simple and useful additional biomaxker of subclinical inflammatory states in patients with stenosis of cerebral arteries. I
I Tu-P 10:447 I P L A T E L E T A C T I V A T I O N A N D B I O C H E M I C A L i M A R K E R S O F I N F L A M M A T I O N IN P A T I E N T S WITH DEPRESSION AND CORONARY HEART DISEASE L.I. Bouriachkovskaia, E.O. Poliakova, A.V. Zorin, I.A. UchiteL N.L. Dovlatova, A.B. Sumarokov, V.E Masenko, T.V. Kuznetsova. Russian
Cardiology Research Complex, Moscow, Russia Objective: Investigation of platelet morphology and activity: their connection with the levels of biochemical markers of inflammation in patients (pts) with coronary heart disease (CHD) and depression. Methods: 14 CHD pts with depression and 19 CHD pts without clinical signs of inflammation were included in the study. 16 healthy subjects (HS) were taken as a control. The levels of IL-2, IL-6, TNF-alpha and s V C A M were measured by immune-enzyme analysis and the level of hCRP was assessed by the laser nephelometer. The concentration of serotonin was measured by liquid chromatography. Platelet aggregation was investigated simultaneously by analysis of fluctuations of optical transmission and by turbidometric method with "BIOLN. Platelets form, leukocyte-platelet (LPA) and erythrocyte-platelet EPA) aggregates were assessed by scanning electron microscopy. Results: Spontaneous aggregation (SPA) (p<0,05), appearance of reticulated platelets (0,74-0,2 CHD pts vs 1,94-0,5% CHD/depression pts) and increase in number of proplatelets (2,34-0,8 in HS vs 5,54-2,9% CHD pts vs 7,14-2,6% CHD/depression pts) were the most representative. SPA was elevated in 12 pts, while ADP-induced was elevated only in 5 pts. The platelet's serotonin was decreased in CHD/depression pts (p<0,05), whereas serotonin-induced platelet aggregation did not rise in the majority of pts. LPA and EPA appeared in the pts, and their number was significantly higher in CHD/depression pts comparing to CHD pts. The levels of IL-2, IL-6 and s V C A M were significantly higher in pts with comparison to HS, and the level of TNF-alpha remained within the normal values. The concentration of CRP was significantly elevated in both groups of pts, but also did not exceed the upper border of normal range in none of the pts. Conclusion: Increased platelet activity in CHD pts with depression together with the development of inflammation indicates an increased of thrombotic risk in patients with CHD and depression. I
I T u - P 1 0 : 4 4 8 iI HS- C R P L E V E L IN R E L A T I O N W I T H S E V E R I T Y O F C O R O N A R Y L E S I O N S IN P A T I E N T S U N D E R G O I N G DIAGNOSTIC CORONARY ANGIOGRAPHY A. Bulo ] , N. Heta ] , E. Refatllaxi ] , I. Korita ] , A. Goda 2, A. Doko 2, L. Simaku 2, I. Temah -. Laboratory Department Uhc Mother Teresa, Tirana,
Albania: 2 Cardiology Department, Uhc Mother Teresa, Tirana, Albania Objectives: Inflammation plays a major role in the initiation, progression, and destabilization of atheromas. High-sensitivity C-reactive protein (hs-CRP) is a drculating acute-phase reactant that reflects active systemic inflammation. The investigation of the relationship between hs-CPR as a marker of systemic inflammation and the severity of angiographically detectable coronary atherosderotic lesions is the aim of this study. Methods: We study 176 patients (55-4-9years) who underwent diagnostic coronary angiography.Serum samples were assayed for hs-CRRwith use of chemiluminescent immunometric assay on a DPC IMMULITE 1000 analyser.The patients was divided in 4 group:G1 with 0% vassel stenosis (n=40),G2 single-vessel stenosis(n=41),G3 double-vessel stenosis (n=48),G4 three-vessel stenosis (n=47). Results: For the whole group of patients the hs-CRP mean value result 10.874-13.1 mg/L. hs-CRP level result higher in vessel stenosis patients compared with no vessel stenosis group (12.464-15.31 vs7.064-8.77mg/L, p=0.034).There was a significant difference between hs-CRP levels in G1 group compared with G3 and G4 groups (7.064-8.77 vs. 15.794-18.74 and 13.474-14.31 rag/L, p <0.05), but the difference between G1 and G2, G3
283
and G4 groups was not significant (7.06-t-8.77 vs 7.42-t-9.40mg/L, p=0.863), (15.79-t-18.74 vs 13.47-t-14.31 mg/L, p=0.504).A weak correlation between hs-CRP and number of vessel stenosis was observed (r=0.218) Conclusion: hs-CRP level is not d e a r l y associated with the angiographic severity of coronary artery disease. F u n d i n g : Laboratory Depaxtment,UHC MotherTeresa Tirana,Albania
I Tu-P10:449 I ERYTHROCYTE SEDIMENTATION RATE AND CORONARY ATHEROSCLEROSIS J. Auer, T. Weber, R. Berent, G. Lamm, W. Stainer, G. Krennmadr, B. Eber.
Department of Cardiology, Wels, Austria B a c k g r o u n d : Various indices of systemic inflammation have been reported to predict coronary artery disease. The erythrocyte sedimentation rate (ESR) largely reflects the plasma concentration of acute phase response proteins resulting in a compound index of both viscosity and inflammation. However, relatively scarce information is available on the association of ESR with the anatomical status of the coronary circulation. The aim of the present study was to test whether the ESR is related to the presence of coronary atherosclerosis assessed by coronary angiography. Methods: 240 consecutive patients referred for diagnostic coronary angiography underwent a thorough physical examination, routine laboratory testing, and cardiac evaluation. ESR measurement by the Westergren method was performed for all patients. The coronary angiograms were evaluated in a blinded manner. Any coronary stenosis with a diameter reduction of fifty percent or more was considered as CAD. Results: 149 (62.1%) had CAD assessed by coronary angiography. BSG was 16 (-4-17.2) and 11.1 (-4-11.3) mm/h in patients with and without CAD, respectively (p=0.01). ESR was progressively higher in the presence of 1, 2/3-vessel disease (13.4-t-13.3; 16.9 -t-18.3; p<0.01). Age-and sex-adjusted ESR was positively related to CAD both in univariate analysis (OR 1.02 [ 1.001-1.05] per 1 mm/h) and in a multivariate model (including principal risk factors such as total cholesterol, LDL-C, hypertension; OR 1.02 [1.001-1.05] per 1 mm/h). C o n d n s i o n : ESR is an independent correlate of presence of CAD in consecutive patients referred for diagnostic coronary angiography.
Tu-P10:4501 S I M I L A R I T Y A N D D I F F E R E N C E
BETWEEN OSTEOPROTEGERIN (OPG) AND OSTEOPONTIN (OPN) L E V E L S IN P A T I E N T S R E C E I V I N G C O R O N A R Y R O T A T I O N A L A T H E R E C T O M Y (RA)
H. Akao, M. Kitayama, H. Uenishi, T. Kasuga, M. Asano, R. Sato, A. Motoyama, M. Wakasa, C. Kitaoka, K. Kajinami. Department of
Cardiology, Kanazawa Medical Universi~, Ishikxtwa, Japan OPG, an inhibitor of osteoclastogenesis, is reported to have a role in atherosclerosis. OPN contributes to smooth muscle cell proliferation as well as vascular calcification. To investigate the hypothesis that RA may release molecules from vessel wall into blood, we measured peripheral levels of OPG and OPN before and after RA, and investigate the determinant of their levels with special reference with inflammatory biomaxkers. We enrolled consecutive 46 patients (mean age: 70 years, M/F=28/18) treated with RA. OPN levels (mean-t-SD, ng/ml) significantly (p<0.001) increased immediately after RA (from 695-t-293 to 886-t-343), further increased to 1197-t-513 three hours later, and returned to 749-t-310 at the time of 24 hours after procedure. Contrary, OPG levels (pmol/1) remained unchanged, (6.0-t-4.7, 6.1-t-4.1, 4.9-t-3.6, and 4.9-t-3.5). Preprocedural levels of OPG showed positive association with those of OPN (r=0.526, p =0.0002), and showed modest associations with hsCRP(r=0.417, p =0.004) and IL6(r=0.235, p =0.116), while OPN levels were highly associated with these markers; hsCRP(r=0.499, p =0.0006) and IL6(r=0.520, p =0.0003). These results suggested that both OPG and OPN levels axe associated with vascular inflammation, but these two molecules may play different roles in coronary atherosclerotic lesions.
XIV bzterTtational Symposium on Atherosclerosis, Rome, Italy, June 18-22, 2006
I Tu-P 10:447 I P L A T E L E T A C T I V A T I O N A N D B I O C H E M I C A L i M A R K E R S O F I N F L A M M A T I O N IN P A T I E N T S WITH DEPRESSION AND CORONARY HEART DISEASE L.I. Bouriachkovskaia, E.O. Poliakova, A.V. Zorin, I.A. UchiteL N.L. Dovlatova, A.B. Sumarokov, V.E Masenko, T.V. Kuznetsova. Russian
Cardiology Research Complex, Moscow, Russia Objective: Investigation of platelet morphology and activity: their connection with the levels of biochemical markers of inflammation in patients (pts) with coronary heart disease (CHD) and depression. Methods: 14 CHD pts with depression and 19 CHD pts without clinical signs of inflammation were included in the study. 16 healthy subjects (HS) were taken as a control. The levels of IL-2, IL-6, TNF-alpha and s V C A M were measured by immune-enzyme analysis and the level of hCRP was assessed by the laser nephelometer. The concentration of serotonin was measured by liquid chromatography. Platelet aggregation was investigated simultaneously by analysis of fluctuations of optical transmission and by turbidometric method with "BIOLN. Platelets form, leukocyte-platelet (LPA) and erythrocyte-platelet EPA) aggregates were assessed by scanning electron microscopy. Results: Spontaneous aggregation (SPA) (p<0,05), appearance of reticulated platelets (0,74-0,2 CHD pts vs 1,94-0,5% CHD/depression pts) and increase in number of proplatelets (2,34-0,8 in HS vs 5,54-2,9% CHD pts vs 7,14-2,6% CHD/depression pts) were the most representative. SPA was elevated in 12 pts, while ADP-induced was elevated only in 5 pts. The platelet's serotonin was decreased in CHD/depression pts (p<0,05), whereas serotonin-induced platelet aggregation did not rise in the majority of pts. LPA and EPA appeared in the pts, and their number was significantly higher in CHD/depression pts comparing to CHD pts. The levels of IL-2, IL-6 and s V C A M were significantly higher in pts with comparison to HS, and the level of TNF-alpha remained within the normal values. The concentration of CRP was significantly elevated in both groups of pts, but also did not exceed the upper border of normal range in none of the pts. Conclusion: Increased platelet activity in CHD pts with depression together with the development of inflammation indicates an increased of thrombotic risk in patients with CHD and depression. I
I T u - P 1 0 : 4 4 8 iI HS- C R P L E V E L IN R E L A T I O N W I T H S E V E R I T Y O F C O R O N A R Y L E S I O N S IN P A T I E N T S U N D E R G O I N G DIAGNOSTIC CORONARY ANGIOGRAPHY A. Bulo ] , N. Heta ] , E. Refatllaxi ] , I. Korita ] , A. Goda 2, A. Doko 2, L. Simaku 2, I. Temah -. Laboratory Department Uhc Mother Teresa, Tirana,
Albania: 2 Cardiology Department, Uhc Mother Teresa, Tirana, Albania Objectives: Inflammation plays a major role in the initiation, progression, and destabilization of atheromas. High-sensitivity C-reactive protein (hs-CRP) is a drculating acute-phase reactant that reflects active systemic inflammation. The investigation of the relationship between hs-CPR as a marker of systemic inflammation and the severity of angiographically detectable coronary atherosderotic lesions is the aim of this study. Methods: We study 176 patients (55-4-9years) who underwent diagnostic coronary angiography.Serum samples were assayed for hs-CRRwith use of chemiluminescent immunometric assay on a DPC IMMULITE 1000 analyser.The patients was divided in 4 group:G1 with 0% vassel stenosis (n=40),G2 single-vessel stenosis(n=41),G3 double-vessel stenosis (n=48),G4 three-vessel stenosis (n=47). Results: For the whole group of patients the hs-CRP mean value result 10.874-13.1 mg/L. hs-CRP level result higher in vessel stenosis patients compared with no vessel stenosis group (12.464-15.31 vs7.064-8.77mg/L, p=0.034).There was a significant difference between hs-CRP levels in G1 group compared with G3 and G4 groups (7.064-8.77 vs. 15.794-18.74 and 13.474-14.31 rag/L, p <0.05), but the difference between G1 and G2, G3
283
and G4 groups was not significant (7.06-t-8.77 vs 7.42-t-9.40mg/L, p=0.863), (15.79-t-18.74 vs 13.47-t-14.31 mg/L, p=0.504).A weak correlation between hs-CRP and number of vessel stenosis was observed (r=0.218) Conclusion: hs-CRP level is not d e a r l y associated with the angiographic severity of coronary artery disease. F u n d i n g : Laboratory Depaxtment,UHC MotherTeresa Tirana,Albania
I Tu-P10:449 I ERYTHROCYTE SEDIMENTATION RATE AND CORONARY ATHEROSCLEROSIS J. Auer, T. Weber, R. Berent, G. Lamm, W. Stainer, G. Krennmadr, B. Eber.
Department of Cardiology, Wels, Austria B a c k g r o u n d : Various indices of systemic inflammation have been reported to predict coronary artery disease. The erythrocyte sedimentation rate (ESR) largely reflects the plasma concentration of acute phase response proteins resulting in a compound index of both viscosity and inflammation. However, relatively scarce information is available on the association of ESR with the anatomical status of the coronary circulation. The aim of the present study was to test whether the ESR is related to the presence of coronary atherosclerosis assessed by coronary angiography. Methods: 240 consecutive patients referred for diagnostic coronary angiography underwent a thorough physical examination, routine laboratory testing, and cardiac evaluation. ESR measurement by the Westergren method was performed for all patients. The coronary angiograms were evaluated in a blinded manner. Any coronary stenosis with a diameter reduction of fifty percent or more was considered as CAD. Results: 149 (62.1%) had CAD assessed by coronary angiography. BSG was 16 (-4-17.2) and 11.1 (-4-11.3) mm/h in patients with and without CAD, respectively (p=0.01). ESR was progressively higher in the presence of 1, 2/3-vessel disease (13.4-t-13.3; 16.9 -t-18.3; p<0.01). Age-and sex-adjusted ESR was positively related to CAD both in univariate analysis (OR 1.02 [ 1.001-1.05] per 1 mm/h) and in a multivariate model (including principal risk factors such as total cholesterol, LDL-C, hypertension; OR 1.02 [1.001-1.05] per 1 mm/h). C o n d n s i o n : ESR is an independent correlate of presence of CAD in consecutive patients referred for diagnostic coronary angiography.
Tu-P10:4501 S I M I L A R I T Y A N D D I F F E R E N C E
BETWEEN OSTEOPROTEGERIN (OPG) AND OSTEOPONTIN (OPN) L E V E L S IN P A T I E N T S R E C E I V I N G C O R O N A R Y R O T A T I O N A L A T H E R E C T O M Y (RA)
H. Akao, M. Kitayama, H. Uenishi, T. Kasuga, M. Asano, R. Sato, A. Motoyama, M. Wakasa, C. Kitaoka, K. Kajinami. Department of
Cardiology, Kanazawa Medical Universi~, Ishikxtwa, Japan OPG, an inhibitor of osteoclastogenesis, is reported to have a role in atherosclerosis. OPN contributes to smooth muscle cell proliferation as well as vascular calcification. To investigate the hypothesis that RA may release molecules from vessel wall into blood, we measured peripheral levels of OPG and OPN before and after RA, and investigate the determinant of their levels with special reference with inflammatory biomaxkers. We enrolled consecutive 46 patients (mean age: 70 years, M/F=28/18) treated with RA. OPN levels (mean-t-SD, ng/ml) significantly (p<0.001) increased immediately after RA (from 695-t-293 to 886-t-343), further increased to 1197-t-513 three hours later, and returned to 749-t-310 at the time of 24 hours after procedure. Contrary, OPG levels (pmol/1) remained unchanged, (6.0-t-4.7, 6.1-t-4.1, 4.9-t-3.6, and 4.9-t-3.5). Preprocedural levels of OPG showed positive association with those of OPN (r=0.526, p =0.0002), and showed modest associations with hsCRP(r=0.417, p =0.004) and IL6(r=0.235, p =0.116), while OPN levels were highly associated with these markers; hsCRP(r=0.499, p =0.0006) and IL6(r=0.520, p =0.0003). These results suggested that both OPG and OPN levels axe associated with vascular inflammation, but these two molecules may play different roles in coronary atherosclerotic lesions.
XIV bzterTtational Symposium on Atherosclerosis, Rome, Italy, June 18-22, 2006