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P53 in normal endometrium and endometrial carcinoma
IAP 2014 ABSTRACTS
Materials and results: Six patients, age range 39–71 years, two on tamoxifen treatment, presented with vaginal bleeding. Four had uterine intracavitary polypoid tumors; one originated in the isthmus and involved the endocervical canal. Only one was located in the ovary. Tumors ranging in size from 2.5 to 19 cm were all in stage 1. Morphologically, all had prominent UTROSCT-like components that comprised at least 25% of tumor volume (criteria for stromal overgrowth); only one was associated with superficial myometrial involvement (1–2 mm). Immunohistochemically, the UTROSCT component was invariably positive for CAM 5.2, CD 56, Inhibin, Calretinin, AR, PR and a-actin. All patients were alive without disease at 8–84 months. Conclusions: Compared to adenosarcomas overgrown by highgrade sarcoma, UTROSCT overgrowth is low-grade and is not associated with recurrence or metastasis in this small series. Therefore, extensive UTROSCT-like growth in the context of adenosarcoma should not be equated with stromal overgrowth in these tumors. Gynecologic Pathology: Poster#165 THE UTILITY OF MOLECULAR AND IMMUNOHISTOCHEMICAL TECHNIQUES IN DIAGNOSING MOLAR PREGNANCIES Katerina Kubelka-Sabit1, Gorgi Bozinovski2, Dzengis Jashar1, Vanja Filipovski1 and Dijana Plaseska-Karanfilska2 1Clinical Hospital Acibadem Sistina, Skopje, Macedonia, and 2Research Center for Genetic Engineering and Biotechnology Georgi D Efremov, Macedonian Academy of Sciences and Arts, Macedonia Based on established morphological and cytogenetic criteria, molar pregnancy is divided into partial and complete. The risk of persistent trophoblastic disease is higher in complete moles. The aim of this study was to assess the importance of additional molecular and immunohistochemical methods to accurately determine the presence and type of molar pregnancy. This study examined 30 cases of hydropic abortion and molar pregnancies, which were also analyzed with immunohistochemical methods using p57 and p63 antibodies. Molecular analysis of the placental tissue was done in 25 of the cases. The results showed that chromosomal abnormalities were found in 82% of the cases. Of these, 27.3% showed triploidy, whereas maternal tissue was not found in one analyzed case of complete mole. Trisomy was found in 40% and 9% were consistent with Turner syndrome. The immunohistochemical analysis revealed absence of p57 positivity in both cases of complete mole, but there was no difference in p63 positivity between the different molecular groups. In order to obtain accurate and clinically relevant diagnosis of molar pregnancy, the products of conception should be analyzed by an experienced pathologist, using additional immunohistochemical and molecular analyses. Gynecologic Pathology: Poster#166 TUMOUR SUPPRESSOR GENE AND PROTEIN TP53/P53 IN NORMAL ENDOMETRIUM AND ENDOMETRIAL CARCINOMA Vladimir Sisovsky1,2,3,4, Zora Lasabova5, Lubomir Straka6, Miroslava Telkova5, Boris Rychly7, Gabriel Minarik4,8, Karina Gemzova5,9, Robert Petrovic10, Tomas Szemes4, Jan Turna4, Vanda Repiska10 and Ludovit Danihel1,2,3
S91
1Institute of Pathological Anatomy, Faculty of Medicine,
Comenius University in Bratislava, Bratislava, Slovak Republic, 2Department of Pathology, University Hospital Bratislava, Bratislava, Slovak Republic, 3Pathological-Anatomical Workplace, Health Care Surveillance Authority, Bratislava, Slovak Republic, 4Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic, 5Department of Molecular Biology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovak Republic, 6Klinicka patologia Presov, s.r.o., Presov, Slovak Republic, 7Cytopathos, s.r.o., Bratislava, Slovak Republic, 8Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic, 9Institute of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovak Republic, and 10Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, University Hospital Bratislava, Bratislava, Slovak Republic Endometrial carcinoma (ECa) is the most common neoplasia of the female genital system. There are two basic types of ECa, endometrioid (estrogen related, indolent) and non-endometrioid (estrogen unrelated, aggressive). The tp53 is a tumour suppressor gene (17p13) encoding a DNA-binding phosphoprotein P53, 53 kDa, a transcription factor that mediates the cell’s response to various kinds of genotoxic stress by preventing cell division and/or inducing DNA excise repair or apoptosis. Tp53 alterations results in an aberrant P53 with a longer half-life that accumulates in the cell. A total of 40 archived formalin-fixed and paraffin-embedded human biopsy tissue specimens with normal proliferative endometrium, endometrioid grade G1 and G3 and serous (SC) subtype of ECa were evaluated by immunohistochemistry for P53 expression/ accumulation in nuclei of endometrial-epithelial cells; and by molecular biology methods for the exons 5-8 tp53 DNA sequence alterations. The expression/accumulation of tp53/P53 was related only to aggressive (mainly SC) types of ECa. Tp53 mutations (substitutions) were detected in SC only. There is no expression/ accumulation of tp53/P53 in normal endometrium. The tp53/P53 expression/accumulation and tp53 mutations presence are associated with aggressive type of ECa. Evaluation of tp53/P53 in ECa could be a relevant component useful in research and clinical practice.
Gynecologic Pathology: Poster#167 VAGINAL CARCINOMA AFTER HYSTERECTOMY FOR INVASIVE HYDATIDIFORM MOLE: A DIAGNOSTIC DILEMMA BETWEEN EPITHELIOID TROPHOBLASTIC TUMOR AND VAGINAL CANCER Nopporn Satabongkoch1, Sunida Rewsuwan1, Kornkanok Sukpan1, Kittipak Charoenkwan2 and Surapan Khunamornpong1 1Department of Pathology, and 2Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand Vaginal cancer is an uncommon type of gynecologic malignancy. The occurrence of vaginal carcinoma after a hysterectomy in the absence of a prior cervical epithelial lesion is very uncommon, and that after a hysterectomy for hydatidiform mole is extremely rare. Hydatidiform mole is a well-recognized precursor lesion for
Copyright © Royal College of pathologists of Australasia. Unauthorized reproduction of this article is prohibited.
Materials and results: Six patients, age range 39–71 years, two on tamoxifen treatment, presented with vaginal bleeding. Four had uterine intracavitary polypoid tumors; one originated in the isthmus and involved the endocervical canal. Only one was located in the ovary. Tumors ranging in size from 2.5 to 19 cm were all in stage 1. Morphologically, all had prominent UTROSCT-like components that comprised at least 25% of tumor volume (criteria for stromal overgrowth); only one was associated with superficial myometrial involvement (1–2 mm). Immunohistochemically, the UTROSCT component was invariably positive for CAM 5.2, CD 56, Inhibin, Calretinin, AR, PR and a-actin. All patients were alive without disease at 8–84 months. Conclusions: Compared to adenosarcomas overgrown by highgrade sarcoma, UTROSCT overgrowth is low-grade and is not associated with recurrence or metastasis in this small series. Therefore, extensive UTROSCT-like growth in the context of adenosarcoma should not be equated with stromal overgrowth in these tumors. Gynecologic Pathology: Poster#165 THE UTILITY OF MOLECULAR AND IMMUNOHISTOCHEMICAL TECHNIQUES IN DIAGNOSING MOLAR PREGNANCIES Katerina Kubelka-Sabit1, Gorgi Bozinovski2, Dzengis Jashar1, Vanja Filipovski1 and Dijana Plaseska-Karanfilska2 1Clinical Hospital Acibadem Sistina, Skopje, Macedonia, and 2Research Center for Genetic Engineering and Biotechnology Georgi D Efremov, Macedonian Academy of Sciences and Arts, Macedonia Based on established morphological and cytogenetic criteria, molar pregnancy is divided into partial and complete. The risk of persistent trophoblastic disease is higher in complete moles. The aim of this study was to assess the importance of additional molecular and immunohistochemical methods to accurately determine the presence and type of molar pregnancy. This study examined 30 cases of hydropic abortion and molar pregnancies, which were also analyzed with immunohistochemical methods using p57 and p63 antibodies. Molecular analysis of the placental tissue was done in 25 of the cases. The results showed that chromosomal abnormalities were found in 82% of the cases. Of these, 27.3% showed triploidy, whereas maternal tissue was not found in one analyzed case of complete mole. Trisomy was found in 40% and 9% were consistent with Turner syndrome. The immunohistochemical analysis revealed absence of p57 positivity in both cases of complete mole, but there was no difference in p63 positivity between the different molecular groups. In order to obtain accurate and clinically relevant diagnosis of molar pregnancy, the products of conception should be analyzed by an experienced pathologist, using additional immunohistochemical and molecular analyses. Gynecologic Pathology: Poster#166 TUMOUR SUPPRESSOR GENE AND PROTEIN TP53/P53 IN NORMAL ENDOMETRIUM AND ENDOMETRIAL CARCINOMA Vladimir Sisovsky1,2,3,4, Zora Lasabova5, Lubomir Straka6, Miroslava Telkova5, Boris Rychly7, Gabriel Minarik4,8, Karina Gemzova5,9, Robert Petrovic10, Tomas Szemes4, Jan Turna4, Vanda Repiska10 and Ludovit Danihel1,2,3
S91
1Institute of Pathological Anatomy, Faculty of Medicine,
Comenius University in Bratislava, Bratislava, Slovak Republic, 2Department of Pathology, University Hospital Bratislava, Bratislava, Slovak Republic, 3Pathological-Anatomical Workplace, Health Care Surveillance Authority, Bratislava, Slovak Republic, 4Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic, 5Department of Molecular Biology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovak Republic, 6Klinicka patologia Presov, s.r.o., Presov, Slovak Republic, 7Cytopathos, s.r.o., Bratislava, Slovak Republic, 8Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic, 9Institute of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovak Republic, and 10Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, University Hospital Bratislava, Bratislava, Slovak Republic Endometrial carcinoma (ECa) is the most common neoplasia of the female genital system. There are two basic types of ECa, endometrioid (estrogen related, indolent) and non-endometrioid (estrogen unrelated, aggressive). The tp53 is a tumour suppressor gene (17p13) encoding a DNA-binding phosphoprotein P53, 53 kDa, a transcription factor that mediates the cell’s response to various kinds of genotoxic stress by preventing cell division and/or inducing DNA excise repair or apoptosis. Tp53 alterations results in an aberrant P53 with a longer half-life that accumulates in the cell. A total of 40 archived formalin-fixed and paraffin-embedded human biopsy tissue specimens with normal proliferative endometrium, endometrioid grade G1 and G3 and serous (SC) subtype of ECa were evaluated by immunohistochemistry for P53 expression/ accumulation in nuclei of endometrial-epithelial cells; and by molecular biology methods for the exons 5-8 tp53 DNA sequence alterations. The expression/accumulation of tp53/P53 was related only to aggressive (mainly SC) types of ECa. Tp53 mutations (substitutions) were detected in SC only. There is no expression/ accumulation of tp53/P53 in normal endometrium. The tp53/P53 expression/accumulation and tp53 mutations presence are associated with aggressive type of ECa. Evaluation of tp53/P53 in ECa could be a relevant component useful in research and clinical practice.
Gynecologic Pathology: Poster#167 VAGINAL CARCINOMA AFTER HYSTERECTOMY FOR INVASIVE HYDATIDIFORM MOLE: A DIAGNOSTIC DILEMMA BETWEEN EPITHELIOID TROPHOBLASTIC TUMOR AND VAGINAL CANCER Nopporn Satabongkoch1, Sunida Rewsuwan1, Kornkanok Sukpan1, Kittipak Charoenkwan2 and Surapan Khunamornpong1 1Department of Pathology, and 2Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand Vaginal cancer is an uncommon type of gynecologic malignancy. The occurrence of vaginal carcinoma after a hysterectomy in the absence of a prior cervical epithelial lesion is very uncommon, and that after a hysterectomy for hydatidiform mole is extremely rare. Hydatidiform mole is a well-recognized precursor lesion for
Copyright © Royal College of pathologists of Australasia. Unauthorized reproduction of this article is prohibited.