Type 2 diabetes increases the risk for gout in Taiwan

Poster Presentations / Diabetes Research and Clinical Practice 120S1 (2016) S65–S211

Results: A total of 985 was initially identified (Pubmed = 420, Web of Science = 546, CENTRAL = 19). After removed duplicate articles (n = 292) and irrelevant articles (n = 669), there were 24 articles selected for full text review and 11 articles left to be included in present analysis. Overall, patients who are diagnosed of OSA had a higher chance of CKD with pooled odds ratio of 2.01 (95% C.I: 1.61–2.2); however, the risk was increased with pooled odds ratio of 2.46 (95% C.I: 2.0–3.03) in patients with diabetes mellitus. In addition, we found that OSA was consistently associated with a higher proteinuria/ albuminuria and a worse renal function with pooled odds ratio of 2.44 (95% C.I: 2.12–2.80), 1.50 (95% C.I: 1.21–1.86) respectively. Conclusion: Our report demonstrated that OSA is significantly associated with CKD, which may be particular important to patients with diabetes mellitus. PJ-49 The development of human recombinant protein, GAPtin, for diabetes Shih Ting TSENG1,2 *, Chia Shin YANG3, Yeh CHEN3. 1 Department of Endocrinology and Metabolism, Kuang Tien General Hospital, 2Department of Food and Nutrition, Providence University, 3 Department of Biotechnology, Hungkuang University, Taiwan Obesity has become a growing epidemic that plaques people of different cultures worldwide. Obesity is such a major health concern because it leads to several fatal diseases. Obesity sufferers are prone to cardiovascular problems, diabetes, stroke and heart attack. To study these diseases such as obesity and diabetes, many reports reveals that some proteins and/or peptides could reduce the blood-glucose level and inhibit appetite. The major issues are the half-life is very short of critical polypeptides molecules, and they were very sensitive to proteinases and were degraded by these enzymes. In order to increase the half-life of these proteins, three polypeptides, Adiponectin, Glucagon-like peptide-1, Peptide YY, will be selected and genetic fused to form a fusion protein, GAPtin. The structural and biochemical assays will be performed to confirm the protein folding, protein sensitivities, and protein functions. In further, we proposed that the abilities of blood sugar control and appetite inhibition of these fusion proteins could be elevate by using animal studies. We hope that these recombinant proteins not only can be used in reducing blood-glucose levels of diabetes patients but also can prevent the obesity-related diseases. The air sensitivity of latter groups makes the expression and purification of such proteins challenging. Here we describe a method for the purification of the GAPtin protein under conditions. Our procedure consisted of three steps. Subsequent affinity chromatography on Ni-column, anion exchange on Q-column, and gel filtration on Superdex 200. Structure and functionality tests performed with mass spectrometry and circular dichroism spectroscopy assays approved the success of the purification procedure. We have already purified GAPtin protein. PJ-50 Type 2 diabetes increases the risk for gout in Taiwan Shih Chueh CHEN1, Kuo Ting SUN2 *. 1Department of Endocrinology and Metabolism, Cheng Ching Hospital, Taichung, 2 Depatment of Dentistry, China Medical University Hospital Taichung, Taiwan Through impaired kidney ammoniagenesis and because a low urine PH, type 2 diabetes may be the main factor of hyperuricemia and gout formation. It was hypothesized that type 2 diabetes should favor the formation of gout. Therefore, in a series of 257,156 patients with type 2 diabetes and 1,078,773 without type 2 diabetes. The proportion of gout was 13.4% in patients with type 2 diabetes and 11.3% in patients without type 2 diabetes (Adjusted HR 1.28 (1.23, 1.34), P < 0.001). Multivariable analysis included age (Adjusted HR 1.45 (1.37, 1.53), P < 0.001), sex (Adjusted HR 2.00 (1.93, 2.08),

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P < 0.001), and comorbidities of chronic liver disease and cirrhosis (Adjusted HR 1.20 (1.15, 1.26), P < 0.01), hypertension (Adjusted HR 1.86(1.78,1 .94), P < 0.001), hyperlipidemia (Adjusted HR 1.53(1.46, 1.59), P < 0.001), stroke (Adjusted HR 0.91(0.82, 1.01), P > 0.05), coronary artery disease (Adjusted HR 1.07(1.02, 1.13), P < 0.001), chronic kidney disease (Adjusted HR 1.44(1.35, 1.53), P < 0.001), and obesity (Adjusted HR 1.09 (0.93, 1.28), P < 0.01). All above variables except stroke had elevated hazard risk for gout formation significantly. There were still significant higher risks for gout between patients with type 2 diabetes and patients without type 2 diabetes after stratified by age, sex and comorbidity. Stepwise regression analysis identified type 2 diabetes as a strong factor that was independently associated with the risk for gout. The occurrence of gout was most apparent in obese men and in patients at the oldest age and hyperlipidemia. The 10-year cummulative incidence of gout for patients with type 2 diabetes was about 1.6 times that without type 2 diabetes. In conclusion, in view of the strong association between type 2 diabetes and gout formation, it is proposed that gout may be added to the conditions that potentially are associated with insulin resistance. Accordingly, it is suggested that patients with gout, especially if overweight, should be screened for the presence of type 2 diabetes or components of the metabolic syndrome. PJ-51 Impact of metabolic status on the incidence of psoriasis: A Korean nationwide cohort study Eun Sook KIM1, Min Ji KIM1, Sung Dae MOON1, Hyuk-Sang KWON1 *. 1The Catholic University of Korea, Korea Background: Growing evidence suggests that obesity is a risk factor for incident psoriasis. This study was aimed to evaluate the association of obesity and metabolic status with the incidence of psoriasis. Methods: A total of 418,057 adults were followed-up using a nationwide prospective cohort study in Korea. Participants were stratified based on the body mass index categories and metabolic condition. Results: During the follow-up visit, 11,054 (2.6%) cases were found to have psoriasis. Diabetes, hypertension, hyperlipidemia, and obesity were all found to be risk factors for incident psoriasis. Subjects with the metabolically unhealthy nonobese phenotype (MUNO; hazard ratio [HR], 1.29; 95% confidence interval [CI], 1.22–1.37) and metabolically unhealthy obese phenotype (MUO; HR, 1.33; 95% CI, 1. 26–1.41) had a significantly higher risk of psoriasis incidence as compared to those with the metabolically healthy non-obese phenotype, after adjusting for age, sex, smoking, exercise, and income. The risk of psoriasis development was found to be high among those with the MUNO and MUO phenotypes in both sexes and all age groups. Conclusions: The metabolic health status was significantly associated with an increased risk of psoriasis in both obese and non-obese individuals. However, further studies are needed to evaluate whether the control of metabolic parameters can lower the incidence of psoriasis. PJ-52 Extra virgin olive oil does not cause post-prandial endothelial dysfunction unlike processed butter through protective mechanisms against oxidative stress Yuji TAJIRI1 *, Kentaro YAMADA1. 1Division of Endocrinology and Metabolism, Kurume University School of Medicine, Japan It is well known that Traditional Mediterranean Diet (TMD), characterized by a high consumption of vegetables, legumes, grains, fruits, nuts and olive oil, reduces blood pressure and produces benefits on cardiovascular (CV) risk. Previous reports demonstrated that the intake of extra virgin olive oil (EVOO), rich in polyphenol compounds, exerted a protective effect