- Email: [email protected]
Ultrasonographic Evaluation of Transplanted Kidneys: Establishing a Protocol
S92
Ultrasound in Medicine and Biology
to prevent manifest renal damage and to postpone the onset of irreversible diabetic nephropathy.
TL.12.005 Ultrasonographic Evaluation of Transplanted Kidneys: Establishing a Protocol R. M. Mendonc¸a, I. X. C. R. Rebouc¸as, J. C. D. Oliveira, E. A. Q. Lisboa, M. A. Borba, A. A. L. Liguori, Z. J. Queiroz, J. B. Almeida Hospital Universit ario Onofre Lopes, Natal, Rio Grande Do Norte, Brasil Brief Description of the Purpose of the Study: Kidney transplantation is an important therapy in chronic kidney disease and anticipate the urological/vascular/functional complications is crucial in maintaining graft. The Doppler ultrasonography (USG-Dp) plays an important role in this follow-up, being requested to patients with clinical intercurrences. This study aims to define a protocol of USG-Dp routine, even for patients with adequate evolution, will show benefits, anticipating behaviors in patients with subclinical changes. Methods: Receptors patients underwent two tests routinely performed by the same sonographer in the 2nd and 7th postoperative day. Registered ultrasound findings, clinical outcome and was noted if the USG-Dp modified behavior. Main Results: Included 29 patients (mean age 43.14), 27 cadaver donor receivers. 75.8% (22) were classified as clinical expected, considering postoperative acute tubular necrosis related to ischemic time. Of the total, 24.1% (07) had significant ultrasound findings, altering therapy applied. Isolating only those patients with postoperative compatible course, which would not be assessed at this time, 18.2% (04) had the follow-up clinical modified/anticipated based on this USG-Dp, implemented as a routine. Importance of the Conclusions: Based on the results it is essential to establish protocol USG-Dp in transplant patients, even within the normal clinical course, providing basis for behavior change in 24.1% of cases, helping to prevent tragic outcomes.
13 - New Technologies in Ultrasound PD.13.001 WITHDRAWN
TL.13.012 Micro/Nano Silica Shell Particle for Ultrasound Imaging Z. Wu, A. Liberman, H. P. Martinez, Y. Kono, R. Mattrey, W. Trogler, A. Kummel University of California, San Diego CA, USA Brief Description of the Purpose of the Study: We develop a gas filled silica shell particle as a novel ultrasound contrast agent. There is an increased need for submicron ultrasound contrast agents with extended period of lifetime for various reasons. The micro/nano shells (M-NSs) can be injected via IV or subcutaneously and imaged up to 7 days post injection. The particle’s acoustic behavior is studied and in vivo imaging is demonstrated. Methods: We manufactured hollow silica particles in sizes ranging from 100 nm to 2000 nm and filled them with perfluoropentane vapor. The M-Ns are imaged with SEM and TEM for their morphology and size distribution. They are also observed by contrast specific (CPS mode) ultrasound and Doppler ultrasound in vitro. For in vitro studies, 100nm, 500nm, and 2um diameter particles are dispersed
Volume 39, Number 5S, 2013 in degassed water and placed in a thin wall plastic chamber. A Siemens Sequoia scanner was used to scan the sample at various Mechanical Indexes (MI). A minimum MI for each type of particles was determined by observing a minimally visible signal with the ultrasound imaging system. Main Results: SEM and TEM images for two different sizes of hollow microshells that have been prepared. The high degree of reproducibility of the gas-filled microshells in the bulk sample and their dispersal from one another were documented. In the in vitro study, under the CPS mode with a transmit frequency of 7MHz, a minimum MI is found to excite this particle to render visible signals. Strong ultrasound enhancement in the peritoneal cavity is observed when a rat is injected with 200ug of 500nm particles intrapertoneally and imaged at high MI up to 72hr post injection. Importance of the Conclusions: We have developed a novel submicron silica shell particle which lasts days in tissue with strong signals in CPS mode. This is the first demonstration of an ultrasound contrast agent which can persist for several days in tissue.
TL.13.015 New Computer Technology - Real-Time Virtual Sonography : First Experience of Combination of Ultrasound, CT and MRI A. Zubarev, A. Fedorova, N. Fedorova, S. Churkina President Medical Centre Brief Description of the Purpose of the Study: The objective of this study was to determine diagnostic capabilities and accuracy of virtual sonography in combination with CT and MRI data. Methods: Real-time virtual sonography (RVS) allows to receive simultaneous ultrasound image on the monitor to get a computer image on ultrasound device, corresponding CT or MRI slices. We gained the first clinical experience of RVS in difficult diagnostic cases, when it was necessary to interpret examination data based on different methods of diagnosis. We have examined more than 20 patients, for whom combined examination using CT, MRI and US of liver, kidneys and pelvis, was of a great interest. Main Results: Using technology RVS, we received key information in determining the nature of volume formations in large number of cases. Application of RVS provided additional information and significantly improved diagnostic accuracy. With the possibility of multiple repetition of diagnostic studies, we can combine ultrasound method and CT, performed only once. Thus, in some cases we were able to manage without use of contrast agents in CT and get an equivalent information by ultrasound. Importance of the Conclusions: Combination of different methods, using RVS technology, raises the level of objectivity of ultrasound to a new level, while ultrasound does not lose its main advantages.
TL.13.017 Broadband Attenuation and Size Measurements of Ultrasound Contrast Agents J. L. Raymond,1 K. J. Haworth,1 K. B. Bader,1 K. Radhakrishnan,1 S.-L. Huang,2 D. D. Mcpherson,2 C. K. Holland1 1 University of Cincinnati, Cincinnati, Ohio, USA, 2University of Texas Health Science Center At Houston, Houston, Texas, USA Brief Description of the Purpose of the Study: Echogenic liposomes (ELIP) are under development as an ultrasound contrast agent (UCA) for image-guided, targeted drug delivery. The aim of this study was to investigate the effect of the lipid formulation and type of encapsulated gas on the acoustic response of ELIP under physiologic conditions. The
Ultrasound in Medicine and Biology
to prevent manifest renal damage and to postpone the onset of irreversible diabetic nephropathy.
TL.12.005 Ultrasonographic Evaluation of Transplanted Kidneys: Establishing a Protocol R. M. Mendonc¸a, I. X. C. R. Rebouc¸as, J. C. D. Oliveira, E. A. Q. Lisboa, M. A. Borba, A. A. L. Liguori, Z. J. Queiroz, J. B. Almeida Hospital Universit ario Onofre Lopes, Natal, Rio Grande Do Norte, Brasil Brief Description of the Purpose of the Study: Kidney transplantation is an important therapy in chronic kidney disease and anticipate the urological/vascular/functional complications is crucial in maintaining graft. The Doppler ultrasonography (USG-Dp) plays an important role in this follow-up, being requested to patients with clinical intercurrences. This study aims to define a protocol of USG-Dp routine, even for patients with adequate evolution, will show benefits, anticipating behaviors in patients with subclinical changes. Methods: Receptors patients underwent two tests routinely performed by the same sonographer in the 2nd and 7th postoperative day. Registered ultrasound findings, clinical outcome and was noted if the USG-Dp modified behavior. Main Results: Included 29 patients (mean age 43.14), 27 cadaver donor receivers. 75.8% (22) were classified as clinical expected, considering postoperative acute tubular necrosis related to ischemic time. Of the total, 24.1% (07) had significant ultrasound findings, altering therapy applied. Isolating only those patients with postoperative compatible course, which would not be assessed at this time, 18.2% (04) had the follow-up clinical modified/anticipated based on this USG-Dp, implemented as a routine. Importance of the Conclusions: Based on the results it is essential to establish protocol USG-Dp in transplant patients, even within the normal clinical course, providing basis for behavior change in 24.1% of cases, helping to prevent tragic outcomes.
13 - New Technologies in Ultrasound PD.13.001 WITHDRAWN
TL.13.012 Micro/Nano Silica Shell Particle for Ultrasound Imaging Z. Wu, A. Liberman, H. P. Martinez, Y. Kono, R. Mattrey, W. Trogler, A. Kummel University of California, San Diego CA, USA Brief Description of the Purpose of the Study: We develop a gas filled silica shell particle as a novel ultrasound contrast agent. There is an increased need for submicron ultrasound contrast agents with extended period of lifetime for various reasons. The micro/nano shells (M-NSs) can be injected via IV or subcutaneously and imaged up to 7 days post injection. The particle’s acoustic behavior is studied and in vivo imaging is demonstrated. Methods: We manufactured hollow silica particles in sizes ranging from 100 nm to 2000 nm and filled them with perfluoropentane vapor. The M-Ns are imaged with SEM and TEM for their morphology and size distribution. They are also observed by contrast specific (CPS mode) ultrasound and Doppler ultrasound in vitro. For in vitro studies, 100nm, 500nm, and 2um diameter particles are dispersed
Volume 39, Number 5S, 2013 in degassed water and placed in a thin wall plastic chamber. A Siemens Sequoia scanner was used to scan the sample at various Mechanical Indexes (MI). A minimum MI for each type of particles was determined by observing a minimally visible signal with the ultrasound imaging system. Main Results: SEM and TEM images for two different sizes of hollow microshells that have been prepared. The high degree of reproducibility of the gas-filled microshells in the bulk sample and their dispersal from one another were documented. In the in vitro study, under the CPS mode with a transmit frequency of 7MHz, a minimum MI is found to excite this particle to render visible signals. Strong ultrasound enhancement in the peritoneal cavity is observed when a rat is injected with 200ug of 500nm particles intrapertoneally and imaged at high MI up to 72hr post injection. Importance of the Conclusions: We have developed a novel submicron silica shell particle which lasts days in tissue with strong signals in CPS mode. This is the first demonstration of an ultrasound contrast agent which can persist for several days in tissue.
TL.13.015 New Computer Technology - Real-Time Virtual Sonography : First Experience of Combination of Ultrasound, CT and MRI A. Zubarev, A. Fedorova, N. Fedorova, S. Churkina President Medical Centre Brief Description of the Purpose of the Study: The objective of this study was to determine diagnostic capabilities and accuracy of virtual sonography in combination with CT and MRI data. Methods: Real-time virtual sonography (RVS) allows to receive simultaneous ultrasound image on the monitor to get a computer image on ultrasound device, corresponding CT or MRI slices. We gained the first clinical experience of RVS in difficult diagnostic cases, when it was necessary to interpret examination data based on different methods of diagnosis. We have examined more than 20 patients, for whom combined examination using CT, MRI and US of liver, kidneys and pelvis, was of a great interest. Main Results: Using technology RVS, we received key information in determining the nature of volume formations in large number of cases. Application of RVS provided additional information and significantly improved diagnostic accuracy. With the possibility of multiple repetition of diagnostic studies, we can combine ultrasound method and CT, performed only once. Thus, in some cases we were able to manage without use of contrast agents in CT and get an equivalent information by ultrasound. Importance of the Conclusions: Combination of different methods, using RVS technology, raises the level of objectivity of ultrasound to a new level, while ultrasound does not lose its main advantages.
TL.13.017 Broadband Attenuation and Size Measurements of Ultrasound Contrast Agents J. L. Raymond,1 K. J. Haworth,1 K. B. Bader,1 K. Radhakrishnan,1 S.-L. Huang,2 D. D. Mcpherson,2 C. K. Holland1 1 University of Cincinnati, Cincinnati, Ohio, USA, 2University of Texas Health Science Center At Houston, Houston, Texas, USA Brief Description of the Purpose of the Study: Echogenic liposomes (ELIP) are under development as an ultrasound contrast agent (UCA) for image-guided, targeted drug delivery. The aim of this study was to investigate the effect of the lipid formulation and type of encapsulated gas on the acoustic response of ELIP under physiologic conditions. The