UP-3.192: The Value and Limitations of Intravesical Prostaglandin E2 (PGE2)-Induced Bladder Hyperactivity as an Overactive Bladder Model in Normal, Conscious Sprague-Dawley Rats

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were collected after resection of the transitional zone of the prostate and specimens transported to the laboratory in sterile saline. Homogenized specimens were incubated for 7 days. The patients divided into 2 groups (group 1: culture positive, group 2: culture negative), and compared with prostate volume, prostate calculi, serum PSA, International Prostate Symptom Score (I-PSS), maximum flow rate (Qmax), residual urine volume and ratio to the transitional zone prostate of total prostate (transitional zone ratio). Results: Mean age was 72 years and mean serum PSA was 4.36ng/dl. Group 1 was 7 patients (11.1%) and group 2 was 57 patients (88.9%). There was no significantly differences in age, I-PSS, serum PSA, Qmax, residual urine volume, prostate volume, prostate calculi, but transitional zone ratio was higher in group 1 (45%) than in group 2 (30%) (p⬍0.05). Conclusion: About 11% of the prostate tissue culture showed bacteria growth. The transitional zone ratio was high in patients with bacteria growth. That means bacteria in prostate could be associated with transitional zone growth in patient with benign prostatic hyperplasia. UP-3.192 The Value and Limitations of Intravesical Prostaglandin E2 (PGE2)Induced Bladder Hyperactivity as an Overactive Bladder Model in Normal, Conscious Sprague-Dawley Rats Yoon S, Seong D, Lee T Inha Uiversity Hospital, Incheon, South Korea Introduction and Objective: Establishing an appropriate animal model is essential for investigating the yet unknown mechanisms of overactive bladder (OAB). Prostanoids are an already well known intrinsic cause of overactive bladder in both animal and human. Awake animal models with prostanoids are already being used as an OAB model, but there is no standardization of methods, and especially for the concentration of the administrated prostanoids. So in this study, we tried to objectively establish the standardized concentration of prostanoids and its effect on urination through urodynamic studies with using non-anesthetized Dawley rats. Materials and Methods: We divided 18 female rats (215-280g) into 3 groups of six rats each and we injected 30␮M or 100␮M of PGE2, respectively. A catheter was placed inside the bladder through an incision in the abdominal wall. After three days, cystometry was performed in an awake state. During cystometry, the saline

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without or with PGE2 at 3 different concentrations (30, 50, 100␮M) was administrated into the bladder (10ml/h). Results: Each group of the 30, 50, 100␮M PGE2 administered rats showed an increased level of the basal pressure, the threshold pressure and the maximal pressure compared to the state before administration of PGE2. The bladder capacity, voided volume and micturition interval decreased by a statistically acceptable amount, like was seen in the OAB model. There was a trend that showed a greater increase in the pressure parameters and a greater decrease in the volume parameters in the 50␮M PGE2 group compared to the 30␮M PGE2 group, but there were no differences between the 50␮M and 100␮M groups. Conclusions: In the normal awake rats, PGE2 induced overactive bladder to a statistically significant amount for all concentrations (30, 50, 100␮M). Among these groups, the concentration of 50␮M provoked OAB most effectively, and the higher concentration of PGE2 (100␮M) did not provoke a more efficient OAB, which might have been due to the characteristics of the intrinsic material in the bladders. Thus, we recommend PGE2 50␮M for efficient induction of OAB. UP-3.193 Analysis of Prostatic Calculi Components in Benign Prostatic Hyperplasia Using Chemical Analysis Method Chung J1, Kang P1, Yoon J1, Jung S2 1 Dept. of Urology, Inje University Busan Paik Hospital, Busan, South Korea; 2 Dept. of Urology, Dong-A University Hospital, Busan, South Korea Introduction and Objective: We analyzed the composition of prostatic calculi in benign prostatic hyperplasia to know the mechanism of the formation of the calculus using chemical anlaysis method. Materials and Methods: Between March 2005 and February 2007, 70 prostatic calculi were eligible for stone analysis, after transurethral resection of prostate in benign prostatic hyperplasia with lower urinary tract symptoms. Chemical analyses for calcium, phosphate, uric acid, carbonate, magnesium, ammonia, oxalate and cystine were performed. Results: The prostatic calculi reveals them to be oval or rounded in shape, between 2.0mm and 8.0mm in size. There were 55/70 (78.6%) calculi that were composed of single chemical component, and 15/70 (21.4%) calculi showed mixed composition consisted of carbonate apa-

tite, magnesium ammonium phosphate and uric acid. Carbonate apatite component was most common type of prostatic calculi consisting 52/70 (74.3%) of all calculi, followed by 14/70 (20.0%) of magnesium ammonium phosphate, and 4/70 (5.7%) of calculi had uric acid component. Conclusions: Endogenous components (Carbonate apatite) were commonly found as a major component of the prostatic calculi in benign prostatic hyperplasia. This study suggests that the prostatic calculi in benign prostatic hyperplasia may be formed from stasis of the prostatic fluid due to obstruction of the prostatic duct. UP-3.194 The Outcome of TURP on Lower Urinary Tract Symptoms According to Shape of Prostate Song J, Chung H, Lee H, Chun J, Byun H, Joo J Yonsei University, Wonju College of Medicine, Wonju, South Korea Introduction and Objective: The measurement of prostate volume is not exactly uniform between ultrasound technicians. In contrast, the shape of prostate can be measured relatively consistently between technicians. After transurethral resection of prostate (TURP), Patients without intravesical protrusion of prostate have generally good results. However, there is little data on this finding. We tried to determine the effect of shape on TURP outcome. Materials and Methods: We evaluated 138 patients with TURP in benign prostate hyperplasia (BPH) by single surgeon between January 2006 and April 2008. Each group was divided by prostate shape on transrectal ultrasonography (group I: only transition zone hyperplasia without retrourethral protrusion, group II: with retrourethral protrusion). Various parameters such as International Prostate Symptom Score (IPSS), maximal flow rate, shape, prostate volume, transition zone volume, resection time and resection volume were evaluated and compared. Results: There was no significant difference at age, prostate volume, pre-operation IPSS, pre-operation maximal flow rate and resection volume. But, IPSS changes after TURP were significant between two groups. IPSS scores were more decreased in group I. Conclusions: Group I (only transition zone hyperplasia without retrouretral protrusion) was significantly improved symptom after TURP than group II. We think

UROLOGY 74 (Supplment 4A), October 2009