- Email: [email protected]
USA - Glucose biosensors
Biosensors & Bioelectronics Vol. 11 No. 5 (1996)
determined by the laboratory method and Uf-urea assayed by the biosensor was -1.07 mg/dl and the 95% confidence interval ranged from -2.01 to 0.13 mg/dl. The mean difference between laboratory plasma urea and Uf-urea from the biosensor was on average -1.9 mg/dl and the estimated limits of agreement with a confidence of 95% were -3.16 and 0.64 mg/dl. Comparison between kinetic models and experimental-profiles of plasma urea decrease, evaluations of recirculation and post-dialytic rebound, the role of Kt/V on-line during dialysis were the preliminary clinical applications of this biosensor. Contact: Divisione di Nefrologia, Ospedale San Carlo, Via Pio II, 3, 1-20154 Milano, Italy. Italy - Amperometric sensor for choline and acetylcholine
In ANALYST (120/11 (2731-2736) 1995) A. Guerrieri, G.E. De Benedetto, F. Palmisano & P.G. Zambonin of Universita della Basilicata report on 'Amperometric sensor for choline and aeetyleholine based on a platinum electrode modified by a co-crosslinked bienzymic system' A fast-response and sensitive amperometric biosensor for choline and acetylcholine based on choline oxidase and acetylcholinesterase immobilized on a platinum electrode by co-crosslinking with bovine serum albumin and glutaraldehyde has been developed. The immobilization procedure proved fast and simple, not requiring dedicated manipulation. Detection limits were in the sub-mieromolar range and linearity extended over three concentration decades. After one month of discontinuous use, 70% of the initial sensitivity was still observed. The response time was around 1 s for both analytes making the developed biosensor a suitable detector for flow injection systems and high-performance liquid chromatography. Contact: Dipartimento di Chimica, Universita della Basilicata, 85-85100 Potenza, ltaly. USA - Glucose biosensors
InANN. BIOMED. ENG. (23/6 (833-839) 1995) S. Yang, P. Atanasov & E. Wilkins of the
University of New Mexico report on 'Glucose biosensors based on oxygen electrode with sandwich-type membranes'. A glucose biosensor based on an arnperometric oxygen electrode has been developed. Polycarbonate and Silastic membranes were assembled (glued together) to form a multilayer sandwich glucose diffusion barrier. The effects of the glue layer composition and thickness of the Silastic membrane on sensor response parameters have been investigated in order to optimize the sensor. The parameters measured were the sensitivity, the concentration range of the linear dependence of the sensor response to glucose, and the long-term operation time. The sensors with the sandwich-type glucose diffusion membrane (Silastic membrane prepared from 20% Silastic suspension, glue layer prepared from polyurethane, 0.5 w/v % in THF solution and standard polycarbonate membrane) demonstrated linearity of response up to 520 mg/dl glucose at 25°C and up to 400 mg/dl at 37°C. These sensors showed good reproducibility of response without significant interferenceeffects (from 1 to 5% of the background current value). The long term continuous operational time of the sensors was over 40 days at 37°C, and over 60 days at 25°C. Contact: Chemical~Nuclear Engg. Department, Farris Engineering Center, University o f New Mexico, Albuquerque, N M 87131-1341, USA. USA - Quartz crystal microbalance detection of Vibrio cholerae O139 serotype
In J. IMMUNOL. METHODS (187/1 (121-125) 1995) R.M. Carter, J.J. Mekalanos, M.B. Jacobs, G.J. Lubrano & G.G. Guilbault of Universal Sensors, Inc., report on 'Quartz crystal microbalance detection of Vibrio cholerae O 139 serotype'. A piezoelectric (PZ) quartz crystal microbalance (QCM) biosensor for the rapid detection of Vibrio cholerae serotype O139 has been developed. The antibody to this serotype was immobilizedon the gold transducer surface of a 10 MHZ AT cut PZ crystal. Solutions containing known antigen concentrations were then incubated
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determined by the laboratory method and Uf-urea assayed by the biosensor was -1.07 mg/dl and the 95% confidence interval ranged from -2.01 to 0.13 mg/dl. The mean difference between laboratory plasma urea and Uf-urea from the biosensor was on average -1.9 mg/dl and the estimated limits of agreement with a confidence of 95% were -3.16 and 0.64 mg/dl. Comparison between kinetic models and experimental-profiles of plasma urea decrease, evaluations of recirculation and post-dialytic rebound, the role of Kt/V on-line during dialysis were the preliminary clinical applications of this biosensor. Contact: Divisione di Nefrologia, Ospedale San Carlo, Via Pio II, 3, 1-20154 Milano, Italy. Italy - Amperometric sensor for choline and acetylcholine
In ANALYST (120/11 (2731-2736) 1995) A. Guerrieri, G.E. De Benedetto, F. Palmisano & P.G. Zambonin of Universita della Basilicata report on 'Amperometric sensor for choline and aeetyleholine based on a platinum electrode modified by a co-crosslinked bienzymic system' A fast-response and sensitive amperometric biosensor for choline and acetylcholine based on choline oxidase and acetylcholinesterase immobilized on a platinum electrode by co-crosslinking with bovine serum albumin and glutaraldehyde has been developed. The immobilization procedure proved fast and simple, not requiring dedicated manipulation. Detection limits were in the sub-mieromolar range and linearity extended over three concentration decades. After one month of discontinuous use, 70% of the initial sensitivity was still observed. The response time was around 1 s for both analytes making the developed biosensor a suitable detector for flow injection systems and high-performance liquid chromatography. Contact: Dipartimento di Chimica, Universita della Basilicata, 85-85100 Potenza, ltaly. USA - Glucose biosensors
InANN. BIOMED. ENG. (23/6 (833-839) 1995) S. Yang, P. Atanasov & E. Wilkins of the
University of New Mexico report on 'Glucose biosensors based on oxygen electrode with sandwich-type membranes'. A glucose biosensor based on an arnperometric oxygen electrode has been developed. Polycarbonate and Silastic membranes were assembled (glued together) to form a multilayer sandwich glucose diffusion barrier. The effects of the glue layer composition and thickness of the Silastic membrane on sensor response parameters have been investigated in order to optimize the sensor. The parameters measured were the sensitivity, the concentration range of the linear dependence of the sensor response to glucose, and the long-term operation time. The sensors with the sandwich-type glucose diffusion membrane (Silastic membrane prepared from 20% Silastic suspension, glue layer prepared from polyurethane, 0.5 w/v % in THF solution and standard polycarbonate membrane) demonstrated linearity of response up to 520 mg/dl glucose at 25°C and up to 400 mg/dl at 37°C. These sensors showed good reproducibility of response without significant interferenceeffects (from 1 to 5% of the background current value). The long term continuous operational time of the sensors was over 40 days at 37°C, and over 60 days at 25°C. Contact: Chemical~Nuclear Engg. Department, Farris Engineering Center, University o f New Mexico, Albuquerque, N M 87131-1341, USA. USA - Quartz crystal microbalance detection of Vibrio cholerae O139 serotype
In J. IMMUNOL. METHODS (187/1 (121-125) 1995) R.M. Carter, J.J. Mekalanos, M.B. Jacobs, G.J. Lubrano & G.G. Guilbault of Universal Sensors, Inc., report on 'Quartz crystal microbalance detection of Vibrio cholerae O 139 serotype'. A piezoelectric (PZ) quartz crystal microbalance (QCM) biosensor for the rapid detection of Vibrio cholerae serotype O139 has been developed. The antibody to this serotype was immobilizedon the gold transducer surface of a 10 MHZ AT cut PZ crystal. Solutions containing known antigen concentrations were then incubated
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