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Use of PMSG antisera in superovulated dairy heifers
THERIOGENOLOGY
USE OF PHSG ANTISERA IN SDPEROWLATED DAIRY HEIFERS H. Callesen, A. Bak, T. Greve, B. Avery, P. Gotfredsen, P. Helm, P. Hyttel, J.O. Pedersen, M. Schmidt, S. Smith and N. Svanborg Department of Reproduction, Royal Veterinary and Agricultural University, 13 Biilowsvej,DK-1870 Frederiksberg C, Denmark
One of the major disadvantages of using PMSG for superovulation of cattle is its long half-life. Postomlatory follicular growth and coinciding high estrogen production may be the result. The use of PMSG antiserum has been reported to minimize or prevent this condition. This trial was conducted to examine the effect of antisera against two PMSG preparations on superovulation of dairy heifers. A total of 129 dairy heifers belonging to one herd were treated between 7 and 13 days after estrus with a single injection of one of two preparations of PMSG (Antex, Leo Pharmaceuticals, Ballerup, Denmark; 2400 IU, i.m.; N=48 or Folligon, Intervet Scandinavia, Skovlunde, Denmark; 2500 IU, i.m.; N=81). At 66 to 72 h after PMSG, the heifers were given a prostaglandin analogue (Estrumat Vet., Coopers Animals Health Ltd., Frederiksberg, Denmark, or Prosolvin Vet., Intervet) and were inseminated 69 to 76 h later. At 125 to 142 h after PMSG administration, PMSG antiserum was given intravenously. The Antex heifers received monoclonal (M, N=14) or polyclonal (P, N=17) Anti-Antex (Leo) or saline as control (N=17), while the Folligon heifers received Neutra-PMSG (Intervet; N=38) or saline (N=43). The heifers were slaughtered 6 or 7 days after insemination and the number of corpora lutea recorded. The number of follicles could be accurately determined in 79 heifers, while this was not possible In 50 heifers (39%) with numerous follicles. Ovulations were found in 110 of the 129 heifers (85%), and the oviducts/uterine horns of these animals were flushed with subseguent morphological evaluation of the recovered eggs. No statistical evaluation was attempted due to the limited number of animals in the Antex group. The results (see table) demonstrated that the beneficial effects of
DMSG antiserum treatment were reduction of the incidence of ovarian overstimulation and improvement of the recovery rate, depending on the It was concluded, however, that no clear type of antiserum used. effect of treatment with PMSG antiserum was demonstrated. FOLLICLE-NUMBER. - det. - det. -N CL N n N (%) 9 923 ANTEX saline 17 1824 8 (47) 1 522 M-Anti-Antex 14 1124 7 (50) 4 (24) P-Anti-Antex 17 14+_4 13 6+1
EGG RECOVERY fl. %=0 T* %N# 14 7+_2341-8 4 10 823 51+_i22 15 9?3 6158 1
saline 43 16+_2 25 9fi POLLIGON Neutra-PMSG 38 2023 25 7~1 /129117?1 79 721 total
39 951 4925 4 32 921 3525 7 110 9+_145f3 18
SOURCE OF
PMSG
anti-serum
18 (42) 13 (34) 50 (39)
emb. 5zl 652 3Cl ___ 423 3?1 421
Leaends:N: nmber of heifers;mean number ('_SEn) of corporalutea (CL),follicles(n) or eggs (#); Folliclenumberdetermined(+ det.) or not (- &et.); fi.: heiferswith owlatmns and flushed; emb.:DO. of transferableembryos. % = no. of eggs recoverea/na. of CL; %=C: no eggs recovered; Acknowledawnts: The work was supported by the Danish Agriculturaland Wterinaq ResearchCouncil, DANOVAI/S, IntervetScandmavia and Leo Phamaceutials.
JANUARY
1989VOL.
31 NO. 1
179
USE OF PHSG ANTISERA IN SDPEROWLATED DAIRY HEIFERS H. Callesen, A. Bak, T. Greve, B. Avery, P. Gotfredsen, P. Helm, P. Hyttel, J.O. Pedersen, M. Schmidt, S. Smith and N. Svanborg Department of Reproduction, Royal Veterinary and Agricultural University, 13 Biilowsvej,DK-1870 Frederiksberg C, Denmark
One of the major disadvantages of using PMSG for superovulation of cattle is its long half-life. Postomlatory follicular growth and coinciding high estrogen production may be the result. The use of PMSG antiserum has been reported to minimize or prevent this condition. This trial was conducted to examine the effect of antisera against two PMSG preparations on superovulation of dairy heifers. A total of 129 dairy heifers belonging to one herd were treated between 7 and 13 days after estrus with a single injection of one of two preparations of PMSG (Antex, Leo Pharmaceuticals, Ballerup, Denmark; 2400 IU, i.m.; N=48 or Folligon, Intervet Scandinavia, Skovlunde, Denmark; 2500 IU, i.m.; N=81). At 66 to 72 h after PMSG, the heifers were given a prostaglandin analogue (Estrumat Vet., Coopers Animals Health Ltd., Frederiksberg, Denmark, or Prosolvin Vet., Intervet) and were inseminated 69 to 76 h later. At 125 to 142 h after PMSG administration, PMSG antiserum was given intravenously. The Antex heifers received monoclonal (M, N=14) or polyclonal (P, N=17) Anti-Antex (Leo) or saline as control (N=17), while the Folligon heifers received Neutra-PMSG (Intervet; N=38) or saline (N=43). The heifers were slaughtered 6 or 7 days after insemination and the number of corpora lutea recorded. The number of follicles could be accurately determined in 79 heifers, while this was not possible In 50 heifers (39%) with numerous follicles. Ovulations were found in 110 of the 129 heifers (85%), and the oviducts/uterine horns of these animals were flushed with subseguent morphological evaluation of the recovered eggs. No statistical evaluation was attempted due to the limited number of animals in the Antex group. The results (see table) demonstrated that the beneficial effects of
DMSG antiserum treatment were reduction of the incidence of ovarian overstimulation and improvement of the recovery rate, depending on the It was concluded, however, that no clear type of antiserum used. effect of treatment with PMSG antiserum was demonstrated. FOLLICLE-NUMBER. - det. - det. -N CL N n N (%) 9 923 ANTEX saline 17 1824 8 (47) 1 522 M-Anti-Antex 14 1124 7 (50) 4 (24) P-Anti-Antex 17 14+_4 13 6+1
EGG RECOVERY fl. %=0 T* %N# 14 7+_2341-8 4 10 823 51+_i22 15 9?3 6158 1
saline 43 16+_2 25 9fi POLLIGON Neutra-PMSG 38 2023 25 7~1 /129117?1 79 721 total
39 951 4925 4 32 921 3525 7 110 9+_145f3 18
SOURCE OF
PMSG
anti-serum
18 (42) 13 (34) 50 (39)
emb. 5zl 652 3Cl ___ 423 3?1 421
Leaends:N: nmber of heifers;mean number ('_SEn) of corporalutea (CL),follicles(n) or eggs (#); Folliclenumberdetermined(+ det.) or not (- &et.); fi.: heiferswith owlatmns and flushed; emb.:DO. of transferableembryos. % = no. of eggs recoverea/na. of CL; %=C: no eggs recovered; Acknowledawnts: The work was supported by the Danish Agriculturaland Wterinaq ResearchCouncil, DANOVAI/S, IntervetScandmavia and Leo Phamaceutials.
JANUARY
1989VOL.
31 NO. 1
179