- Email: [email protected]
Use of rpoB sequences and rep-PCR for phylogenetic study of Anoxybacillus species
S76
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
Tests measuring acute toxicity and genotoxicity thus can be used for optimization of biodegradation technologies. doi:10.1016/j.copbio.2011.05.222
G36 Improvement of sewage sludge hazardous characteristics using physical, physicochemical and biodegradation methods Hana Sezimova 1 , Tomas Sezima 2 , Peter Fecko 3 , Ivana Truxova 2 , Eugen Sikora 2 , Radmila Kucerova 3 , Katerina Malachova 1 , Zuzana Rybkova 1
strains, five strains were subjected to rep-PCR genomic fingerprinting using primer sets corresponding to REP, ERIC, BOX elements. The rpoB gene sequence obtained showed from 75% to 99% similarity, these partial 16S rRNA sequences showed that the percent similarity ranged from 94% to 99%. rep-PCR profiles of Anoxybacillus strains showed that fourteen type strains produced different banding patterns. Comparative sequence analysis of rpoB revealed that rpoB comparison of the type strains of Anoxybacillus was more sensitive than 16S rRNA comparison and provided a better resolution for Anoxybacillus species. Through comparison of the rep-PCR, it was found that BOX-PCR profiles were more distinct for the different strains, including a higher number of bands. doi:10.1016/j.copbio.2011.05.224
1
University of Ostrava, Chittussiho 10, 701 03 Ostrava, Czech Republic T.G. Masaryk Water Research Institute, Macharova 5, 702 00 Ostrava, Czech Republic 3 Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava, Czech Republic 2
E-mail address: [email protected] (H. Sezimova) New developments in the area of wastewater treatment together with increasing numbers of households connected to wastewater treatment plants have resulted in a considerable increase of sewage sludge production. Physical and physicochemical processes applicable within the primary treatment to sewage sludge conditioning are able to reduce its dangerous characteristics. These processes can be used individually or in combination with biodegradation. The aim of our study was to evaluate the use of UV radiation, the electrostatic field, the action of high-heat plasma and combination of these methods for reduction of contamination of sewage sludge with PCBs, PAHs and heavy metals. Another approach included a biodegradation treatment with bacteria. The effects of physical, physicochemical and biodegradation treatments on sewage sludge characteristics were measured both by chemical analysis and with the Vibrio fischeri acute toxicity test. All samples of sewage sludge were toxic before degradation. Combination of physical, physicochemical and biodegradation processes was identified as the most effective method for reducing toxicity of sewage sludge. Combination of physical, physicochemical and biodegradation processes is an innovative approach in biodegradation treatment. It also improves the utilization of sewage sludge after reducing its toxicity. doi:10.1016/j.copbio.2011.05.223
G37 Use of rpoB sequences and rep-PCR for phylogenetic study of Anoxybacillus species Kadriye Inan 1 , Yusuf Belduz 1
Bektas 2 , Sabriye
Canakci 1 , Ali
G38 The effect of some bacteria on calcification in geothermal water pipes Ozlem Baris 1 , Medine Gulluce 1 , Fikrettin Sahin 2 , Furkan Orhan 1 , Derya Yanmis 1 , Tugba Bal 1 , Ahmet Adiguzel 3 1
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey 2 Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Turkey 3 Department of Molecular Biology and Genetic, Faculty of Science, Ataturk University, Erzurum, Turkey E-mail address: [email protected] (O. Baris) An important problem for pipes carrying geothermal water (GW) is calcification. Mineralization intensively carried out thanks to the content and loses temperature of GW while it moves through these pipes. Dealing with this problem, various chemicals are added to curing GW which chance the content and/or pH of it’s and brings more cost for administrators. It is known that the unique factor in calcification is not the dissolved minerals in water and physical elements. In this study it has been investigated the role of bacteria in formation of calcification. In vitro mineralization assays has been carried out by using some bacteria isolated from varied geothermal sources. The results showing that the generated mineralization by thermophilic bacteria is more quick and intense than the one generated by mesophilic bacteria. These results demonstrating that the obstruction of the pipes caused by calcification is accelerated by thermophilic bacteria. Consequently, it is thought that the calcification process can be delayed by providing anti-bacterial ions assistance inside of pipes that will reduce more cost due to added chemicals for the administrators and also the GW which is not used directly will be employed. doi:10.1016/j.copbio.2011.05.225
Osman
1
G39
Department of Biology, Faculty of Sciences, Karadeniz Technical University, Trabzon, Turkey 2 Faculty of Fisheries and Aquatic Sciences, Rize University, Rize, Turkey
Hydroxyl radical interactions with glycine and alanine
E-mail address: [email protected] (K. Inan)
1
The aim of this study was to investigate the applicability of rpoB, rep-PCR to be used as an alternative to 16S rRNA sequence similarity analysis in the thermophilic genus Anoxybacillus. Partial rpoB sequences were generated for the 14 type strains of Anoxybacillus species and six strains of four Anoxybacillus species. The sequences and the phylogenetic tree of rpoB were compared with those obtained from 16S rRNA gene analysis. A total of 14 type
Gulin Urgenc 1 , Cenk Selcuki 2 Biotechnology, Graduate School of Natural and Applied Science, Ege University, Izmir, Turkey 2 Biochemistry, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey E-mail address: [email protected] (G. Urgenc) In our study, the effect of hydroxyl radical on the glycine and alanine have been investigated. OH addition to C␣ and H abstraction by
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
Tests measuring acute toxicity and genotoxicity thus can be used for optimization of biodegradation technologies. doi:10.1016/j.copbio.2011.05.222
G36 Improvement of sewage sludge hazardous characteristics using physical, physicochemical and biodegradation methods Hana Sezimova 1 , Tomas Sezima 2 , Peter Fecko 3 , Ivana Truxova 2 , Eugen Sikora 2 , Radmila Kucerova 3 , Katerina Malachova 1 , Zuzana Rybkova 1
strains, five strains were subjected to rep-PCR genomic fingerprinting using primer sets corresponding to REP, ERIC, BOX elements. The rpoB gene sequence obtained showed from 75% to 99% similarity, these partial 16S rRNA sequences showed that the percent similarity ranged from 94% to 99%. rep-PCR profiles of Anoxybacillus strains showed that fourteen type strains produced different banding patterns. Comparative sequence analysis of rpoB revealed that rpoB comparison of the type strains of Anoxybacillus was more sensitive than 16S rRNA comparison and provided a better resolution for Anoxybacillus species. Through comparison of the rep-PCR, it was found that BOX-PCR profiles were more distinct for the different strains, including a higher number of bands. doi:10.1016/j.copbio.2011.05.224
1
University of Ostrava, Chittussiho 10, 701 03 Ostrava, Czech Republic T.G. Masaryk Water Research Institute, Macharova 5, 702 00 Ostrava, Czech Republic 3 Technical University of Ostrava, 17. listopadu 15, 708 33 Ostrava, Czech Republic 2
E-mail address: [email protected] (H. Sezimova) New developments in the area of wastewater treatment together with increasing numbers of households connected to wastewater treatment plants have resulted in a considerable increase of sewage sludge production. Physical and physicochemical processes applicable within the primary treatment to sewage sludge conditioning are able to reduce its dangerous characteristics. These processes can be used individually or in combination with biodegradation. The aim of our study was to evaluate the use of UV radiation, the electrostatic field, the action of high-heat plasma and combination of these methods for reduction of contamination of sewage sludge with PCBs, PAHs and heavy metals. Another approach included a biodegradation treatment with bacteria. The effects of physical, physicochemical and biodegradation treatments on sewage sludge characteristics were measured both by chemical analysis and with the Vibrio fischeri acute toxicity test. All samples of sewage sludge were toxic before degradation. Combination of physical, physicochemical and biodegradation processes was identified as the most effective method for reducing toxicity of sewage sludge. Combination of physical, physicochemical and biodegradation processes is an innovative approach in biodegradation treatment. It also improves the utilization of sewage sludge after reducing its toxicity. doi:10.1016/j.copbio.2011.05.223
G37 Use of rpoB sequences and rep-PCR for phylogenetic study of Anoxybacillus species Kadriye Inan 1 , Yusuf Belduz 1
Bektas 2 , Sabriye
Canakci 1 , Ali
G38 The effect of some bacteria on calcification in geothermal water pipes Ozlem Baris 1 , Medine Gulluce 1 , Fikrettin Sahin 2 , Furkan Orhan 1 , Derya Yanmis 1 , Tugba Bal 1 , Ahmet Adiguzel 3 1
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey 2 Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Turkey 3 Department of Molecular Biology and Genetic, Faculty of Science, Ataturk University, Erzurum, Turkey E-mail address: [email protected] (O. Baris) An important problem for pipes carrying geothermal water (GW) is calcification. Mineralization intensively carried out thanks to the content and loses temperature of GW while it moves through these pipes. Dealing with this problem, various chemicals are added to curing GW which chance the content and/or pH of it’s and brings more cost for administrators. It is known that the unique factor in calcification is not the dissolved minerals in water and physical elements. In this study it has been investigated the role of bacteria in formation of calcification. In vitro mineralization assays has been carried out by using some bacteria isolated from varied geothermal sources. The results showing that the generated mineralization by thermophilic bacteria is more quick and intense than the one generated by mesophilic bacteria. These results demonstrating that the obstruction of the pipes caused by calcification is accelerated by thermophilic bacteria. Consequently, it is thought that the calcification process can be delayed by providing anti-bacterial ions assistance inside of pipes that will reduce more cost due to added chemicals for the administrators and also the GW which is not used directly will be employed. doi:10.1016/j.copbio.2011.05.225
Osman
1
G39
Department of Biology, Faculty of Sciences, Karadeniz Technical University, Trabzon, Turkey 2 Faculty of Fisheries and Aquatic Sciences, Rize University, Rize, Turkey
Hydroxyl radical interactions with glycine and alanine
E-mail address: [email protected] (K. Inan)
1
The aim of this study was to investigate the applicability of rpoB, rep-PCR to be used as an alternative to 16S rRNA sequence similarity analysis in the thermophilic genus Anoxybacillus. Partial rpoB sequences were generated for the 14 type strains of Anoxybacillus species and six strains of four Anoxybacillus species. The sequences and the phylogenetic tree of rpoB were compared with those obtained from 16S rRNA gene analysis. A total of 14 type
Gulin Urgenc 1 , Cenk Selcuki 2 Biotechnology, Graduate School of Natural and Applied Science, Ege University, Izmir, Turkey 2 Biochemistry, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey E-mail address: [email protected] (G. Urgenc) In our study, the effect of hydroxyl radical on the glycine and alanine have been investigated. OH addition to C␣ and H abstraction by