- Email: [email protected]
UV radiations and human keratinocytes immunomodulating cytokines production: In vitro evaluation of immunoprotective effects of topical sunscreens
S280
Posters
- Pltatobialo~y
natural photoprotection obtained during summer season was 2.4 for polychromatic light and 1.7 for UV-B. The results show low photoprotective response of Czech Caucasian skin to seasonal sunbathing. Therefore the artificial photoprotection (sunscreens) is recommended all summer time. I P402 New data on skin photoprotection P Morganti’, G. Fabrizi’. ‘R&D, MAW SUD S.r.1.; Cuore
Catholic
(Inils
2Saoo
Italy
Absorbed ultraviolet (UV) radiation is responsible for photochemical degradation of the skin. Erythema, photoaging and photocarcinogenesis, largely mediated by Reactive Oxygen Species (ROS) are the most serious results. The aim of this study was to evaluate the protective efficacy of 2 creams and I lotion based on UVA, UVB and IR screens (organic and inorganic chemicals), including also free-radical scavengers such as betaglucan and pholyphenols from Green Tea in order to lowe the concentration of the organic screens. At this purpose SPF (Sun Protection Factor) and ROS were controlled. SPF was evaluated on 20 preselected volunteers (women and men t22 years old with skin type I to III) by the Colipa test method using five combined ultnlux 300-W sunlamps filtered with a l-mm ~4-320 filter and measuring the standard sunlight by a calibrated spectrophotometer test (0.69 mw/cm2 >95%) and products were applied at the rate of 2.00 f 004 mglcm2 on the back area (I cm2 on 5 sites). A wash-off test was also conducted to determine the resistance to water. ROS was evaluated on exposed and unexposed skin by a new spectrophotometer methodology named “d-Roms”. Obtained results (SPF 20 and 30) showed that the used free radical scavengers by decreasing the amount of free radicals caused by irradiation at cutaneous level, make sunscreens highly protective even at low dosages. The controlled decrease in effectiveness of only 20% after IO minutes washing proved that the vehicle is R major factor in determining the protective efficacy. Furthermore, after the irradiation, the skin previously treated with the used creams shows a ROS incidence lowered more than 60%. I P403
UV radiations and human keratinocytes immunomodulating cytokines production: In vitro evaluation of immunoprotective effects of topical sunscreens
M.F. Aries, C. Vaissiere, M. Charveron, Y. Gall. Rechehe Biologic
Derttro-Costtlh’que Cellulaire, 31062
Pierre Toulouse,
Fabre, France
brstitut
Loboratoire
aad photoprotection
cultures. Cells were exposed to UVA (2-10 J/cm2) or UVB (0.01-0.02 J/cm2) light and cultured for either 6 h before RNA preparation or 24 h before cytokine enzyme-linked immunosorbent assay (ELISA). We examined the effects of UV radiation on interleukin 6 (iL6), iL8. VEGF (Vascular Endothelial Growth Factor), TNFcv (Tumor Necrosis Factor), iLl0, TGF/I (Transforming Growth Factor), cytokines involved in inllammation. erythem or immunosuppression. This ia vitro assay showed that sunscreens reversed the UV-induced cellular responses of cytokines production and demonstrated the potential immunoprotective properties of topical sunscreens. I P404
C. Baudouin, C. Vaissiere, L. Viau, M.F. Aries, M. Charveron, Y. Gall. brstitat de Recherclre Dert,lo-Cost,l~tiyue Pierre Fabre, Lnboratoire Mdecirre
Exposure of human skin to solar radiations produces several distinct reversible and irreversible clinical effects including sunburn, immunosuppression, skin aging and the development of skin cancer. Ultraviolet light is a potent inducer of cytokine release and epidermal keratinocytes provide a susceptible target and are triggered to produce a plethora of these mediators which play an important role in local and systemic inflammatory reactions and in modulation of immune responses. The aim of this study was to evaluate UV modulation of cytokine production for the assessment of the immunoprotective capacities of topical sunscreens using human keratinocytes
de Biologic Cellulaire Cutatrde, Rangueil, 31062 Toulouse Cedex,
Facrrltt! France
de
Some chemical agents (psoralens. phenothiazines) are described as photosensitizers. In presence of light (UVA or UVB). these molecules are photoactivated and can become phototoxic. epecially in skin cells. This photosensitization responsible for cytotoxicity could also induce a signifcant photogenotoxicity and then photomutagenesis. We have developed phototoxicity and photogenotoxicity study models. The phototoxic potential of photosensitizers is first determined using the EEClCOLIPA phototoxicity protocol and the photogenotoxic effect of these drugs is measured by a biochemical assay called 3D for “Damaged DNA Detection”. This test is based on DNA damages detection on living cells by the ia vitro reconstitution of the DNA repair system. For two reference drugs (5methoxypsoralen and chlorpromazine) we have calculated phototoxic potential and we have determined the genotoxic effect. Our results confirm those described by other authors. These assays could be considered as in vitro models reproducing the phototoxic effect of the photosensitization. Using these models, we have evaluated the protective efficacy of a high sun protection factor mineral sunscreen in order to determine its capacity to reduce the UVA phototoxic effect on exogen photosensitizers. I P405
de de
Evaluation of a mineral sunscreen protective efficacy on photosensitization models
In vitro models for the evaluation of the protective effect of mineral sunscreen
A. Bel, C. Baudouin, L. Rouch, D. Raynal, M. Charveron, Y. Gall. Institut de Recherclre Dertao-CostntQique Pierre Fabre, Lrrboratoire
de Biologic
Cellulaire,
31062
Toulouse,
France
The UVA and UVB components of solar radiations have been shown to be responsible for skin premature photoaging and photocarcinogenesis. Many cellular elements, especially DNA, constitute a target for the UV radiations. In order to evaluate the protective efficacy of a high sun protection factor mineral sunscreen, we have chosen to follow both proteic and genomic markers: the 72-KD heat shock protein (HSP72) and ~53 pmtein, and the UV-induced DNA damages. HSP and ~53, stress proteins, are considered to play a crucial role in protection from cellular injury. The main DNA damages produced by UVB
Posters
- Pltatobialo~y
natural photoprotection obtained during summer season was 2.4 for polychromatic light and 1.7 for UV-B. The results show low photoprotective response of Czech Caucasian skin to seasonal sunbathing. Therefore the artificial photoprotection (sunscreens) is recommended all summer time. I P402 New data on skin photoprotection P Morganti’, G. Fabrizi’. ‘R&D, MAW SUD S.r.1.; Cuore
Catholic
(Inils
2Saoo
Italy
Absorbed ultraviolet (UV) radiation is responsible for photochemical degradation of the skin. Erythema, photoaging and photocarcinogenesis, largely mediated by Reactive Oxygen Species (ROS) are the most serious results. The aim of this study was to evaluate the protective efficacy of 2 creams and I lotion based on UVA, UVB and IR screens (organic and inorganic chemicals), including also free-radical scavengers such as betaglucan and pholyphenols from Green Tea in order to lowe the concentration of the organic screens. At this purpose SPF (Sun Protection Factor) and ROS were controlled. SPF was evaluated on 20 preselected volunteers (women and men t22 years old with skin type I to III) by the Colipa test method using five combined ultnlux 300-W sunlamps filtered with a l-mm ~4-320 filter and measuring the standard sunlight by a calibrated spectrophotometer test (0.69 mw/cm2 >95%) and products were applied at the rate of 2.00 f 004 mglcm2 on the back area (I cm2 on 5 sites). A wash-off test was also conducted to determine the resistance to water. ROS was evaluated on exposed and unexposed skin by a new spectrophotometer methodology named “d-Roms”. Obtained results (SPF 20 and 30) showed that the used free radical scavengers by decreasing the amount of free radicals caused by irradiation at cutaneous level, make sunscreens highly protective even at low dosages. The controlled decrease in effectiveness of only 20% after IO minutes washing proved that the vehicle is R major factor in determining the protective efficacy. Furthermore, after the irradiation, the skin previously treated with the used creams shows a ROS incidence lowered more than 60%. I P403
UV radiations and human keratinocytes immunomodulating cytokines production: In vitro evaluation of immunoprotective effects of topical sunscreens
M.F. Aries, C. Vaissiere, M. Charveron, Y. Gall. Rechehe Biologic
Derttro-Costtlh’que Cellulaire, 31062
Pierre Toulouse,
Fabre, France
brstitut
Loboratoire
aad photoprotection
cultures. Cells were exposed to UVA (2-10 J/cm2) or UVB (0.01-0.02 J/cm2) light and cultured for either 6 h before RNA preparation or 24 h before cytokine enzyme-linked immunosorbent assay (ELISA). We examined the effects of UV radiation on interleukin 6 (iL6), iL8. VEGF (Vascular Endothelial Growth Factor), TNFcv (Tumor Necrosis Factor), iLl0, TGF/I (Transforming Growth Factor), cytokines involved in inllammation. erythem or immunosuppression. This ia vitro assay showed that sunscreens reversed the UV-induced cellular responses of cytokines production and demonstrated the potential immunoprotective properties of topical sunscreens. I P404
C. Baudouin, C. Vaissiere, L. Viau, M.F. Aries, M. Charveron, Y. Gall. brstitat de Recherclre Dert,lo-Cost,l~tiyue Pierre Fabre, Lnboratoire Mdecirre
Exposure of human skin to solar radiations produces several distinct reversible and irreversible clinical effects including sunburn, immunosuppression, skin aging and the development of skin cancer. Ultraviolet light is a potent inducer of cytokine release and epidermal keratinocytes provide a susceptible target and are triggered to produce a plethora of these mediators which play an important role in local and systemic inflammatory reactions and in modulation of immune responses. The aim of this study was to evaluate UV modulation of cytokine production for the assessment of the immunoprotective capacities of topical sunscreens using human keratinocytes
de Biologic Cellulaire Cutatrde, Rangueil, 31062 Toulouse Cedex,
Facrrltt! France
de
Some chemical agents (psoralens. phenothiazines) are described as photosensitizers. In presence of light (UVA or UVB). these molecules are photoactivated and can become phototoxic. epecially in skin cells. This photosensitization responsible for cytotoxicity could also induce a signifcant photogenotoxicity and then photomutagenesis. We have developed phototoxicity and photogenotoxicity study models. The phototoxic potential of photosensitizers is first determined using the EEClCOLIPA phototoxicity protocol and the photogenotoxic effect of these drugs is measured by a biochemical assay called 3D for “Damaged DNA Detection”. This test is based on DNA damages detection on living cells by the ia vitro reconstitution of the DNA repair system. For two reference drugs (5methoxypsoralen and chlorpromazine) we have calculated phototoxic potential and we have determined the genotoxic effect. Our results confirm those described by other authors. These assays could be considered as in vitro models reproducing the phototoxic effect of the photosensitization. Using these models, we have evaluated the protective efficacy of a high sun protection factor mineral sunscreen in order to determine its capacity to reduce the UVA phototoxic effect on exogen photosensitizers. I P405
de de
Evaluation of a mineral sunscreen protective efficacy on photosensitization models
In vitro models for the evaluation of the protective effect of mineral sunscreen
A. Bel, C. Baudouin, L. Rouch, D. Raynal, M. Charveron, Y. Gall. Institut de Recherclre Dertao-CostntQique Pierre Fabre, Lrrboratoire
de Biologic
Cellulaire,
31062
Toulouse,
France
The UVA and UVB components of solar radiations have been shown to be responsible for skin premature photoaging and photocarcinogenesis. Many cellular elements, especially DNA, constitute a target for the UV radiations. In order to evaluate the protective efficacy of a high sun protection factor mineral sunscreen, we have chosen to follow both proteic and genomic markers: the 72-KD heat shock protein (HSP72) and ~53 pmtein, and the UV-induced DNA damages. HSP and ~53, stress proteins, are considered to play a crucial role in protection from cellular injury. The main DNA damages produced by UVB