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Validity of fluorescent polarization immunoassay of plasma cortisol for use in the DST in prepubertal children
Child
8tOL PSYCSIATRY ~t~ :29:43A- ~85A
|47A
± 209 ng-miWml (Mann-Whitney U -- 173; p = 0.038)i. Both peak GH and GHAt:c respcatses to GRF were blunted in PD compared with NV. Peak GH response was 5.5 ± 8 0 n~ml in PD ~ 8.0 ± 5~9 ng/ml in NV (Mann-Whitney U = 190; p = 0.028L GHAuc following GRF was 188 ± 301 n g - ~ in PD and 294 _ 271 ng-min/ml in NV ~Mann-Whitaey U = 194; p = 0.f~lSL Two of 12 (17%~ PD patients had a positive response Amax GH >- 5 ng/ml) to clonidine and 3 of 12 (25%) ~ a positive response to GRF. One PD patient had a positive response to both challenges. ~ data replicate p r e ~ reports of blunted GH responses of GRF in PD patients.
226 DELAYED ONSET OF POSTTRAUMATIC STRESS DISORDER IS ASSOCIATED WITH CONCOMITANT SCHIZOAFFECTIVE MANIC DISORDER Charles VanValkenburg, M.D., John C. Kluznik, M.D. Nevada Department of Prisons, Carson CiD', NV 8971~2. In a group of 202 veterans with posttraumatic stress disorder, only 13 had an onset of symptoms delayed 6 months or more after the traumatic experience. Of these, six had RDC schizoaffective ~ i c disorder (p = 0.0001). Of the three whose onset of PTSD was delayed by at least 2 years, two had schizo~fective manic disorder (p = 0.0073), and all three had some manic disorder (p = 0.055L The onset of p o s ~ u m a t i c stress disorder is not normally delayed; when it is, this is likely to be a manifestation of manic disorder.
CHILD F r i d a y , M a y 10, 1 : 0 0 - 6 : 0 0 PM
Ponchartrain A
227 VALIDITY OF FLUORESCENT POLARIZATION IMMUNOASSAY OF PLASMA CORTISOL FOR USE IN THE DST IN PREPUBERTAL CHILDREN Shahnour Yaylayan, M.D., Elizabeth Weller, M.D., Ronald We~:ler, M.D., Mary Fristad, Ph.D. The Ohio State U,~iversitb.' Hospital, Columbus, OH 43210. A new assay method to determine plasma or serum cortisol levels, fluorescent polarization immtmoassay (FPIA) technology, has been introduced. It is highiy sensitive and specific for cortisol and it does not use radioactive isotopes. However, its use has not been validated in children. To accomplish this. 21 prepubertal psychiatric inpatients who satisfied DSM-III-R criteria for major depressive disorder were studied. A 0.5mg oral dose of dexamethasone was given at 11 PM and cortisol levels were measured by radioimmunoassay at FPIA at 8 AM and 4 PM the next day from split samples. Children with medical illnesses and those who were on medication known to affect the DST were excluded. Correlation between FPIA and RIA determined cortisol levels was highly significant. Regression analyses demonstrated a significant linear relationship between the two measures. Y(FPIA) = 0.9878 (RIA) - 0.1383. F = 486.69; R 2 = 0.94; p < 0.001). To determine if this degree of association would be found at varying absolute values of cortisol levels, we divided the sample in three: < 3, > 3 < 8, > 8. Results remained consistent ( < 3R e = 0.81, F = 33.18, p < 0.001)4, > 3 < 8 R 2 = 0.77, F = 54.83, p < 0.0001; > 8 Re = 0.89, F = 23.80, p < 0.02). RIA values were consistently higher, such that a value of 5.0 ~g/dl (RIA) corresponded with a value of 4.75 p.g/dl (FPIA).
8tOL PSYCSIATRY ~t~ :29:43A- ~85A
|47A
± 209 ng-miWml (Mann-Whitney U -- 173; p = 0.038)i. Both peak GH and GHAt:c respcatses to GRF were blunted in PD compared with NV. Peak GH response was 5.5 ± 8 0 n~ml in PD ~ 8.0 ± 5~9 ng/ml in NV (Mann-Whitney U = 190; p = 0.028L GHAuc following GRF was 188 ± 301 n g - ~ in PD and 294 _ 271 ng-min/ml in NV ~Mann-Whitaey U = 194; p = 0.f~lSL Two of 12 (17%~ PD patients had a positive response Amax GH >- 5 ng/ml) to clonidine and 3 of 12 (25%) ~ a positive response to GRF. One PD patient had a positive response to both challenges. ~ data replicate p r e ~ reports of blunted GH responses of GRF in PD patients.
226 DELAYED ONSET OF POSTTRAUMATIC STRESS DISORDER IS ASSOCIATED WITH CONCOMITANT SCHIZOAFFECTIVE MANIC DISORDER Charles VanValkenburg, M.D., John C. Kluznik, M.D. Nevada Department of Prisons, Carson CiD', NV 8971~2. In a group of 202 veterans with posttraumatic stress disorder, only 13 had an onset of symptoms delayed 6 months or more after the traumatic experience. Of these, six had RDC schizoaffective ~ i c disorder (p = 0.0001). Of the three whose onset of PTSD was delayed by at least 2 years, two had schizo~fective manic disorder (p = 0.0073), and all three had some manic disorder (p = 0.055L The onset of p o s ~ u m a t i c stress disorder is not normally delayed; when it is, this is likely to be a manifestation of manic disorder.
CHILD F r i d a y , M a y 10, 1 : 0 0 - 6 : 0 0 PM
Ponchartrain A
227 VALIDITY OF FLUORESCENT POLARIZATION IMMUNOASSAY OF PLASMA CORTISOL FOR USE IN THE DST IN PREPUBERTAL CHILDREN Shahnour Yaylayan, M.D., Elizabeth Weller, M.D., Ronald We~:ler, M.D., Mary Fristad, Ph.D. The Ohio State U,~iversitb.' Hospital, Columbus, OH 43210. A new assay method to determine plasma or serum cortisol levels, fluorescent polarization immtmoassay (FPIA) technology, has been introduced. It is highiy sensitive and specific for cortisol and it does not use radioactive isotopes. However, its use has not been validated in children. To accomplish this. 21 prepubertal psychiatric inpatients who satisfied DSM-III-R criteria for major depressive disorder were studied. A 0.5mg oral dose of dexamethasone was given at 11 PM and cortisol levels were measured by radioimmunoassay at FPIA at 8 AM and 4 PM the next day from split samples. Children with medical illnesses and those who were on medication known to affect the DST were excluded. Correlation between FPIA and RIA determined cortisol levels was highly significant. Regression analyses demonstrated a significant linear relationship between the two measures. Y(FPIA) = 0.9878 (RIA) - 0.1383. F = 486.69; R 2 = 0.94; p < 0.001). To determine if this degree of association would be found at varying absolute values of cortisol levels, we divided the sample in three: < 3, > 3 < 8, > 8. Results remained consistent ( < 3R e = 0.81, F = 33.18, p < 0.001)4, > 3 < 8 R 2 = 0.77, F = 54.83, p < 0.0001; > 8 Re = 0.89, F = 23.80, p < 0.02). RIA values were consistently higher, such that a value of 5.0 ~g/dl (RIA) corresponded with a value of 4.75 p.g/dl (FPIA).