Variegate porphyria: Identification of 10 novel mutations in the protoporphyrinogen oxidase gene

ESDR I JSID I SID Abstracts

1354 VARIEGATE

PORPHYRIA:

IDENTIFICATION

OF 10 NOVEL MUTATIONS

IN THE

ON THE

EFFECTS OF OLEANOLIC

CHRONIC

DAMAGES

Sane

De,,atment

of Dermatolow

Tae

mRNA

ULTRAVIOLET

Kim, Kee Zuck

We have previously and elastin Although

phatopratectants

demonstrated

was reduced

that

because

antiox,dati”e

Youne

li’,DUCED

SW

can

that the expression

by all-lrans-retinoic

reduce

of their

and

chemical

improve or

UDCA was

invest&wed

the

acute

admuastercd

and subjected

with

IS unknown. synthesis

cultured

and elashn

The

Lo northern result

steroids.

effect on photoagmg

as skin their

The possibility

by 0.4

human d-al

was

as fallows.

result suggesh

as well as fibroblasts and kept m

Total RNA was isolated

blot analysis

mRNA was reduced

before or after u-radiation. Tb,r

lba”v photowotitx”e and &&in

well as chronic

acid

the effect of OA and UDCA on

and &&in

in this study. Confiuently

as

similarities

properties,

collagenase

and elaetin.

collagenase

Kim

collagenax

acxd(UDCA) have been suggested

media for 24 hours before or after UVA irmdiatmn. for collagenase

Cheol

and ursalic

with 15J/cm2 of UVA. OA and UDCA were administered

fallowing UVA irradiatkx UVA-induced

CUTANEOUS

Hvun

of UVA-mduced

and elasbn synthesis by UVA irradiation of UVA-induced

were rrradiated

Chae.

acid (t-RA)

bmlagical

effect and immunomodulatory

the collagenase

cDNA

RADIATION

S&

and ursodeoxychobc

uf inhibition

culture

ACID AND URSODEOXYCHOLIC

Kosln Mfxhcal College, Pusan, Korea

oleanolic acid(OA)

damage

PROTOPORPHYRIA: Fmnk K. Jugerl’, Joroe

1355

1352 PHOTOPROTECTIVE ACID

MOLECULAR HETEROGENEITY IN ERYTHROPOIETIC 10 MUTATIONS IN THE FERROCHELATASE GENE.

using oligolabelled The

exprrssion

of

when OA and UDCA were that OA and ““CA

“la inhibition of UVA-induced

may

collagenasc

svnthesm

1356 THE EFFECT OF SUN EXPOSURE ON THE DELIVERY AND UTlLLZATlON OF OXYGEN IN SKIN. Weinkauf and Uma Santhanam. Unilever Research, U.S., Edgewater, NJ. Photoaged skin is a result of frequent and cumulative photodamage superimpwed on intrinsic aging. It is characteri&d by rough texture; laxity, w&l&, irregular pigmentation and capillary changes. Little is known about the metabolic changes in skin caused by sun exposure. The present study was initiated to investigate the effect of photodamage on oxygen delivery and utilization in facial skin. The study population consisted of 41 women with various levels of photodamage on the face. Oxygenated hemoglobin levels were measured using a diffuse reflectance spectrophotomda (DRS) to determine oxygen delivery. Oxygen utilization was assessed indirectly using a transcutaneous monitor to measure oxygen and carbon dioxide levels at the surface of the skin. In addition, the amount of blood flow was mdirectly estimated via the milliwatts feature of the transcutaneous monitor. Measurements were taken on the face and compared to a photoprotected site i.e. inner, uo~er arm. No significant differences were observed in milliwatts or oxxenated h&oglobin with &reasing facial photodamage. However, transcutaneou~ oxygen levels were lower (~~0.08) on the facial skin of subiects with mild photodamage compared to moderate photodamage. These results suggest that facial phbtodamageis accompanied by a decrease in skin’s capability to consume oxygen, without an apparent effect on delivery of oxygen to skin. Dramatic differences were observed between measurements taken on the face and on the inner, upper arm. Transcutaneous oxygen levels on facial skin were much lower than on the inner, upper arm and milliwatts and oxygenated hemoglobin measurements were higher on the face than on the arm. These results suggest that blood flow and oxygen utilization xe much higher in facial skin relative to arm skin.