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Volatile fatty acid (VFA) production and cecal development in rats fed diets containing soy fiber
P.47
VOLATILE FATTY ACID (VFA) PRODUCTION AND CECAL DEVELOPMENT SOY FIBER. 'S.R. Behr, 2C. Remesy, and *C. Demigne. Laboratories, Columbus, Ohio, USA. zINRA, Theix, France.
IN RATS FED DIETS CONTAINING IAbbott International/Ross
The fermentation of soluble fiber sources such as pectin provides VFAs which have trophic effects both in the small and large intestines. Unfortunately, the inclusion of a soluble fiber source in liquid formulas is presently technically unfeasible because of the gelljng properties of such fibers. The following experiment-was done to assess the "in vivo" fermentation of soy fiber, a relatively insoluble fiber which can be added to enteral formulas. Male Wistar rats, weighing 160-200 g were fed a fiber free purified diet (P) containinq by weight 18% casein. 5% corn oil. and 71% wheat starch. In the experimental vow, soy fiber (Fibrim TM) was substituted for wheat starch at a level of 30% by weight. Weight gain was normal in both the P and fiber-supplemented (F) groups, averaging 5.2 g/day over the 28 day period of the study (this includes 7 days of adaptation of all rats to the P diet, followed by 3 weeks of measurements on the F diet). In the F rats, total average cecal weight more than doubled, representing an increase in the mean weight of cecal contents from 4 g to 10 g and an increase in cecal wall weight from 1.04 f 0.05 g to 2.28 f 0.06 g (P
P.48 ILEUM
ADAPTATION IN RATS FED WITH WHEY PROTEINS, THEIR CONSTITUTIVE PEPTIDES HYDROLYSATE OR AMINO ACIDS. M.G. Poullain, C. Marche*, L. Roger**, J.P. Broyart, J.P. Cezard. INSERM U120 Le Vesinet *H8p. Cl. Bernard Paris **SOPHARGA RU Paris, France.
Synthetic diets are used frequently in human intestinal pathology of the ileum. The aim of our work was to study the effects of similar synthetic diets which differ only by the molecular form of nitrogen : whole whey proteins (WP), small peptides hydrolysate of WP (WPH) on their free amino acid mix (AAM), on the morphology and brush border disaccharidases (D) and peptidases (P) of the ileum mucosa in 80 Wistar rats (25Og) : 18 controls (C) fed 96h and experimental rats starved for 3 days and refed (R) during 96h (R : 24, 48, 72 and 96h). In C and R 90 Cal/day has to be ingested. Morphometric data show that the AAM diet determined the highest villus height (VH) (PC 0.01) and mitosis/crypt (Ml, as compared to the two other diets, in C and during R. (C (AAM) : VH : 302~8~m, M : 2.620.06, C (WPH) : HV : 27127, M : 1.&0.4, C (WP) : 251+5 M : 2.lrO.3) ; (R : 96H (AAM) : HV : 30125, M : 2.9+0.2, WPH : 251+8, M : 1.220.2, (WP) : HV : 25728, M : 1.220.2). In C, no char& was observed-for N. Aminopeptisase .(NAP) and glucoamylase CC). But significative increases (~(0.05) of sucrase (S), dipeptidyl peptidase (DDPIV), amino acid peptidase (AAP) andyglutamyl transferase (
81
VOLATILE FATTY ACID (VFA) PRODUCTION AND CECAL DEVELOPMENT SOY FIBER. 'S.R. Behr, 2C. Remesy, and *C. Demigne. Laboratories, Columbus, Ohio, USA. zINRA, Theix, France.
IN RATS FED DIETS CONTAINING IAbbott International/Ross
The fermentation of soluble fiber sources such as pectin provides VFAs which have trophic effects both in the small and large intestines. Unfortunately, the inclusion of a soluble fiber source in liquid formulas is presently technically unfeasible because of the gelljng properties of such fibers. The following experiment-was done to assess the "in vivo" fermentation of soy fiber, a relatively insoluble fiber which can be added to enteral formulas. Male Wistar rats, weighing 160-200 g were fed a fiber free purified diet (P) containinq by weight 18% casein. 5% corn oil. and 71% wheat starch. In the experimental vow, soy fiber (Fibrim TM) was substituted for wheat starch at a level of 30% by weight. Weight gain was normal in both the P and fiber-supplemented (F) groups, averaging 5.2 g/day over the 28 day period of the study (this includes 7 days of adaptation of all rats to the P diet, followed by 3 weeks of measurements on the F diet). In the F rats, total average cecal weight more than doubled, representing an increase in the mean weight of cecal contents from 4 g to 10 g and an increase in cecal wall weight from 1.04 f 0.05 g to 2.28 f 0.06 g (P
P.48 ILEUM
ADAPTATION IN RATS FED WITH WHEY PROTEINS, THEIR CONSTITUTIVE PEPTIDES HYDROLYSATE OR AMINO ACIDS. M.G. Poullain, C. Marche*, L. Roger**, J.P. Broyart, J.P. Cezard. INSERM U120 Le Vesinet *H8p. Cl. Bernard Paris **SOPHARGA RU Paris, France.
Synthetic diets are used frequently in human intestinal pathology of the ileum. The aim of our work was to study the effects of similar synthetic diets which differ only by the molecular form of nitrogen : whole whey proteins (WP), small peptides hydrolysate of WP (WPH) on their free amino acid mix (AAM), on the morphology and brush border disaccharidases (D) and peptidases (P) of the ileum mucosa in 80 Wistar rats (25Og) : 18 controls (C) fed 96h and experimental rats starved for 3 days and refed (R) during 96h (R : 24, 48, 72 and 96h). In C and R 90 Cal/day has to be ingested. Morphometric data show that the AAM diet determined the highest villus height (VH) (PC 0.01) and mitosis/crypt (Ml, as compared to the two other diets, in C and during R. (C (AAM) : VH : 302~8~m, M : 2.620.06, C (WPH) : HV : 27127, M : 1.&0.4, C (WP) : 251+5 M : 2.lrO.3) ; (R : 96H (AAM) : HV : 30125, M : 2.9+0.2, WPH : 251+8, M : 1.220.2, (WP) : HV : 25728, M : 1.220.2). In C, no char& was observed-for N. Aminopeptisase .(NAP) and glucoamylase CC). But significative increases (~(0.05) of sucrase (S), dipeptidyl peptidase (DDPIV), amino acid peptidase (AAP) andyglutamyl transferase (
81