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von Willebrand factor and microalbuminuria with the development of metabolic syndrome in Chinese hypertensive patients
Abstracts
pronounced effect on size of left atrium than hypertension, obesity and hypertension have synergistic effect on left atrial enlargement.
doi:10.1016/j.ijcard.2011.08.754 0098 VCAM-1 promoted by leptin could be offset by adiponectin through the activation of AMPK via AdipoR1 receptor of VECS in 3D vessel model ZHI ZHANG, HONGXIAN WU, BAOGUI SUN, QIUYAN DAI Shanghai First People's Hospital, Shanghai, China Objective: To observe whether there is direct antagonistic effects of adiponectin against leptin in 3D blood vessel. Methods: A novel 3D co-culture model was constructed by HUASMCs (human umbilical artery smooth muscle cells) and HUVECs (human umbilical vein endothelial cells) in Transwell chambers. Secretions and transcription of VEGF, t-PA, NO and IL-8 from HUVECs and VCAM-1, MMP-2 and MCP-1 from HUASMCs were detected by Elisa and Real-time PCR among four groups (leptin, leptin + adiponectin, leptin + losartan and control). Expression of AdipoR1 (adiponectin receptor) or AdipoR2 of HUVECs was inhibited using RNAi technology. Changes of intracellular Ca2+ in HUVEC were detected by Fluorescent probe Fluo-3Am. Changes of p-AMPK/AMPK, p-STAT3/STAT3 were tested by western blot. Results: VEGF and NO were increased by leptin and not affected by adding adiponectin or losartan. IL8 were unaffected by leptin but increased by adding adiponectin (P b 0.01). VCAM-1 was increased by leptin but reduced by adding adiponectin (P b 0.01). Results of real-time PCR were the same as ELISA. Receptors of HUVECs could be effectively inhibit by AdipoR1-siRNA and AdipoR2-siRNA with inhibition rates of 71.83 ± 1.45% and 74.89 ± 1.12% (P b 0.01). The effect of adiponectin in increasing IL-8 and reducing VCAM-1 was diminished by the depression of AdipoR1. Adiponectin increased the concentration of intracellular Ca2+ of HUVECs. p-STAT3 was activated by leptin while p-AMPK was activated by adiponectin. Increasing of IL-8 and reducing of VCAM-1 by adiponectin could be inhibited by compound C and partially inhibited by PD98059. Conclusion: Cell growth and secretion in 3D co-culture model were different from the plane culture. Crosstalk between HUVECs and HUASMCs was mediated by IL-8 and it reduced secretion and transcription of VCAM-1via ERK pathway. By activating AdipoR1, adiponectin increased the concentration of intracellular Ca2+, and exerted partial anti-inflammatory effects against Leptin though the AMPK pathway. Adiponectin might also increase the secretion of IL-8 in HUVECs to reduce VCAM-1 from HUASMCs.
doi:10.1016/j.ijcard.2011.08.755 0160 von Willebrand factor and microalbuminuria with the development of metabolic syndrome in Chinese hypertensive patients JING LIUa, JING YANGb, NINGLING SUNa a Peking University People's Hospital, Heart Center, Beijing, China b Peking University People's Hospital, Emergency Department, Beijing, China Objectives: To compare the difference of von Willebrand factor (vWF) and microalbuminuria (MAU) between Chinese hypertensives and hypertensive patients with metabolic syndrome (MS), and to explore the relation of these factors with the development of MS. Methods: According to the MS definition by International Diabetes
S87
Federation (IDF 2005), 133 consecutive Chinese hypertensive subjects were divided into two groups: essential hypertensives (EH group, n= 36) and those with metabolic syndrome (EH + MS group, n= 97). Biochemistry assay, plasma vWF, 24 h urine albumin excretion (UAE) and morning urine albumin/creatinine ratio (ACR) were measured. The association of vWF and MAU with MS was analyzed. Results: Waist circumference, body mass index, triglyceride (TG), low density lipoprotein cholesterol (LDL-C), plasma vWF and UAE were increased (P b 0.05 or P b 0.01, respectively), while high density lipoprotein cholesterol (HDL-C) was decreased (P b 0.01) in EH+ MS group, as compared with those in EH group. UAE was positively correlated with ACR (r= 0.707, P b 0.01). vWF was not correlated with UAE or ACR (r= 0.079 and 0.052, PN0.05, respectively). Logistic regression showed HDL-C, TG, LDL-C,vWF and UAE were associated with the development of MS in hypertensive patients (standardized coefficients: −0.825, 0.63, 0.339, 0.331, 0.371, P b 0.05 or P b 0.01, respectively). Conclusions: Compared with essential hypertensives, the Chinese hypertensive patients with MS had more MAU and serious endothelial dysfunction. vWF and MAU were associated with the development of MS in patients with hypertension. doi:10.1016/j.ijcard.2011.08.756 0209 No association between variation in the ZFP36 gene locus and metabolic traits in Uygur subjects at increased risk for obesity XIAOGUANG YAO, NANFANG LI, HONGMEI WANG, YANYING GUO Hypertension Institute of Xinjiang, Urumqi, China Hypertension Center of The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China Objective: The zinc finger protein 36 (ZFP36) is implicated overexpression in obese men without the metabolic syndrome (MetS). Two ZFP36 polymorphisms were associated with body weight, body mass index (BMI), as well as glucose and LDL-cholesterol levels in a cohort study. ZFP36 gene expression in omental adipose tissue, but not in abdominal s.c. fat, may offer partial protection against the development of insulin resistance and diabetes. Therefore, we investigated in an association study of Uygur subjects at high risk for obesity whether genetic variation within the ZFP36 gene locus contributes to MetS phenotypes. Methods: We genotyped 1021 subjects (647 women, 374 men) for two single nucleotide polymorphisms (SNPs) tagging 100% of common variants (MAF N 0.05) within the ZFP36 gene locus with an r2 ≥ 0.8. All subjects underwent questionnaire, physical examination, measurement of lipid levels, TNF-α in the plasma. Results: After appropriate adjustment and Bonferroni correction for multiple comparisons, none of the rs251864, rs3746083 SNPs was reliably associated with MetS. Multiple logistic regression indicated age [OR (95%CI): 1.02(1.00–1.03), P = 0.02], being female [OR(95%CI): 2.16 (1.61–2.89), P b 0.001] and alcohol intake [OR(95%CI): 2.97 (1.18–7.47), P = 0.02] were significant factors. Conclusions: Our data suggest that common variation within the ZFP36 gene locus may not play a major role in the development of MetS in adult Uygur population in Xinjiang. doi:10.1016/j.ijcard.2011.08.757 0217 The rs4149601 polymorphism of NEDD4L gene is associated with obesity Kazakh general population in Xinjiang HONGMEI WANG, NANFANG LI Hypertension Institute of Xinjiang, Urumqi, China Hypertension Center of The People's Hospital Of Xinjiang Uygur Autonomous Region, Urumqi, China
pronounced effect on size of left atrium than hypertension, obesity and hypertension have synergistic effect on left atrial enlargement.
doi:10.1016/j.ijcard.2011.08.754 0098 VCAM-1 promoted by leptin could be offset by adiponectin through the activation of AMPK via AdipoR1 receptor of VECS in 3D vessel model ZHI ZHANG, HONGXIAN WU, BAOGUI SUN, QIUYAN DAI Shanghai First People's Hospital, Shanghai, China Objective: To observe whether there is direct antagonistic effects of adiponectin against leptin in 3D blood vessel. Methods: A novel 3D co-culture model was constructed by HUASMCs (human umbilical artery smooth muscle cells) and HUVECs (human umbilical vein endothelial cells) in Transwell chambers. Secretions and transcription of VEGF, t-PA, NO and IL-8 from HUVECs and VCAM-1, MMP-2 and MCP-1 from HUASMCs were detected by Elisa and Real-time PCR among four groups (leptin, leptin + adiponectin, leptin + losartan and control). Expression of AdipoR1 (adiponectin receptor) or AdipoR2 of HUVECs was inhibited using RNAi technology. Changes of intracellular Ca2+ in HUVEC were detected by Fluorescent probe Fluo-3Am. Changes of p-AMPK/AMPK, p-STAT3/STAT3 were tested by western blot. Results: VEGF and NO were increased by leptin and not affected by adding adiponectin or losartan. IL8 were unaffected by leptin but increased by adding adiponectin (P b 0.01). VCAM-1 was increased by leptin but reduced by adding adiponectin (P b 0.01). Results of real-time PCR were the same as ELISA. Receptors of HUVECs could be effectively inhibit by AdipoR1-siRNA and AdipoR2-siRNA with inhibition rates of 71.83 ± 1.45% and 74.89 ± 1.12% (P b 0.01). The effect of adiponectin in increasing IL-8 and reducing VCAM-1 was diminished by the depression of AdipoR1. Adiponectin increased the concentration of intracellular Ca2+ of HUVECs. p-STAT3 was activated by leptin while p-AMPK was activated by adiponectin. Increasing of IL-8 and reducing of VCAM-1 by adiponectin could be inhibited by compound C and partially inhibited by PD98059. Conclusion: Cell growth and secretion in 3D co-culture model were different from the plane culture. Crosstalk between HUVECs and HUASMCs was mediated by IL-8 and it reduced secretion and transcription of VCAM-1via ERK pathway. By activating AdipoR1, adiponectin increased the concentration of intracellular Ca2+, and exerted partial anti-inflammatory effects against Leptin though the AMPK pathway. Adiponectin might also increase the secretion of IL-8 in HUVECs to reduce VCAM-1 from HUASMCs.
doi:10.1016/j.ijcard.2011.08.755 0160 von Willebrand factor and microalbuminuria with the development of metabolic syndrome in Chinese hypertensive patients JING LIUa, JING YANGb, NINGLING SUNa a Peking University People's Hospital, Heart Center, Beijing, China b Peking University People's Hospital, Emergency Department, Beijing, China Objectives: To compare the difference of von Willebrand factor (vWF) and microalbuminuria (MAU) between Chinese hypertensives and hypertensive patients with metabolic syndrome (MS), and to explore the relation of these factors with the development of MS. Methods: According to the MS definition by International Diabetes
S87
Federation (IDF 2005), 133 consecutive Chinese hypertensive subjects were divided into two groups: essential hypertensives (EH group, n= 36) and those with metabolic syndrome (EH + MS group, n= 97). Biochemistry assay, plasma vWF, 24 h urine albumin excretion (UAE) and morning urine albumin/creatinine ratio (ACR) were measured. The association of vWF and MAU with MS was analyzed. Results: Waist circumference, body mass index, triglyceride (TG), low density lipoprotein cholesterol (LDL-C), plasma vWF and UAE were increased (P b 0.05 or P b 0.01, respectively), while high density lipoprotein cholesterol (HDL-C) was decreased (P b 0.01) in EH+ MS group, as compared with those in EH group. UAE was positively correlated with ACR (r= 0.707, P b 0.01). vWF was not correlated with UAE or ACR (r= 0.079 and 0.052, PN0.05, respectively). Logistic regression showed HDL-C, TG, LDL-C,vWF and UAE were associated with the development of MS in hypertensive patients (standardized coefficients: −0.825, 0.63, 0.339, 0.331, 0.371, P b 0.05 or P b 0.01, respectively). Conclusions: Compared with essential hypertensives, the Chinese hypertensive patients with MS had more MAU and serious endothelial dysfunction. vWF and MAU were associated with the development of MS in patients with hypertension. doi:10.1016/j.ijcard.2011.08.756 0209 No association between variation in the ZFP36 gene locus and metabolic traits in Uygur subjects at increased risk for obesity XIAOGUANG YAO, NANFANG LI, HONGMEI WANG, YANYING GUO Hypertension Institute of Xinjiang, Urumqi, China Hypertension Center of The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China Objective: The zinc finger protein 36 (ZFP36) is implicated overexpression in obese men without the metabolic syndrome (MetS). Two ZFP36 polymorphisms were associated with body weight, body mass index (BMI), as well as glucose and LDL-cholesterol levels in a cohort study. ZFP36 gene expression in omental adipose tissue, but not in abdominal s.c. fat, may offer partial protection against the development of insulin resistance and diabetes. Therefore, we investigated in an association study of Uygur subjects at high risk for obesity whether genetic variation within the ZFP36 gene locus contributes to MetS phenotypes. Methods: We genotyped 1021 subjects (647 women, 374 men) for two single nucleotide polymorphisms (SNPs) tagging 100% of common variants (MAF N 0.05) within the ZFP36 gene locus with an r2 ≥ 0.8. All subjects underwent questionnaire, physical examination, measurement of lipid levels, TNF-α in the plasma. Results: After appropriate adjustment and Bonferroni correction for multiple comparisons, none of the rs251864, rs3746083 SNPs was reliably associated with MetS. Multiple logistic regression indicated age [OR (95%CI): 1.02(1.00–1.03), P = 0.02], being female [OR(95%CI): 2.16 (1.61–2.89), P b 0.001] and alcohol intake [OR(95%CI): 2.97 (1.18–7.47), P = 0.02] were significant factors. Conclusions: Our data suggest that common variation within the ZFP36 gene locus may not play a major role in the development of MetS in adult Uygur population in Xinjiang. doi:10.1016/j.ijcard.2011.08.757 0217 The rs4149601 polymorphism of NEDD4L gene is associated with obesity Kazakh general population in Xinjiang HONGMEI WANG, NANFANG LI Hypertension Institute of Xinjiang, Urumqi, China Hypertension Center of The People's Hospital Of Xinjiang Uygur Autonomous Region, Urumqi, China