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Xinshuitong capsule ameliorates hypertrophy of cardiomyocytes via aquaporin pathway in the ischemia–reperfusion rat hearts
S54
Abstracts
manner in beating rabbit atria. Conclusions: These findings suggest that cardiac CNP regulates NO production. doi:10.1016/j.ijcard.2011.08.643 0277 Xinshuitong capsule ameliorates hypertrophy of cardiomyocytes via aquaporin pathway in the ischemia–reperfusion rat hearts CHUNJIANG TAN, YANBIN WU, WENLIE CHEN, RUHUI LIN Fujian Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province, China Objective: Aquaporin-1 (AQP1), which was present in both human and rat hearts, mediated the water transport, however, Xinshuitong capsule (XC), an aquaretic herbal compound, whether affects AQP1 pathway remains unclear. Materials and methods: 20 male Sprague– Dawley rats (280 ± 83 g) underwent 30 min ischemia by ligation of anterior coronary artery followed by 2 h reperfusion. Animals were randomly treated with XC (0.5 g/kg i.p, during ischemia and reperfusion) or vehicle with 5 sham rats as controls. The animals were killed at 1, 2, 4, 8 and 16 h after surgery. In vitro, AQP1 gene in cardiomyocytes was knocked down or underwent hypoxia/reoxygenation exposed to XC (10, 20, 40 and 80 μg/mL). The ultrastructure of cardiomyocytes was observed by transmission electron microscope. AQP1 in mRNA and protein expressions were determined for all hearts by laser confocal fluorescence microscopy. Results: Vehicle rats showed a prominent cells edema, mitochondrial swelling associated with crest and membrane disruption with abundant lipid-like structures in the cytoplasm at 1–4 h, and a high incidence of plasma membrane rupture at 8–16 h. With an increase in cell volume, a higher fluorescence of AQP1 was detected in the ischemic center and the border zone than in the noninfarcted area. By contrast, XC can significantly ameliorate cells morphology in vivo and in vitro. Compared to the controls, the XC-treated cells showed more tolerant to hypoxic stress, which related to XC dosage in the hypoxia–reoxygenation experiments. XC can further significantly up-regulate AQP1 mRNA and its protein expressions in the XC-treated rat hearts. Identical results were also detected in the XC-treated hypoxia–reoxygenation cells with a marked increase in AQP1 mRNA and its protein than the control. Knocked down gene AQP1, XC presented little effect on the change in cell size. Conclusions: The data suggest that XC can preserve the profile of cardiomyocytes via up-regulation of AQP1 pathway in the ischemia–reperfusion rat hearts. doi:10.1016/j.ijcard.2011.08.644 0376 Clinical features and therapeutic strategy of 36 cases of dilated cardiomyopathy in children XI YANG, GUIYING LIU Beijing Anzhen Hospital, Beijing, China Objective: To explore the clinical features, treatment effect and prognosis of dilated cardiomyopathy (DCM) in children. Methods: Give a comprehensive analysis of 36 children with DCM in Beijing Anzhen Hospital, including clinical manifestations, biomarkers, electrocardiogram (ECG), X-ray, echocardiography (ECHO), drug selection, therapeutic effect and prognosis. Results: All patients have cardiomegaly and heart failure on admission. 11 cases of heart function II, 14 cases of heart function III and 8 cases of heart function IV. The common symptoms were edema in 16 cases (44.4%), anhelation in 15 cases (41.6%) and anorexia in 15 cases (41.6%). Initial symptoms manifested as palpitations in 7 cases (19.4%) and
respiratory infection in 7 cases (19.4%). Apical murmur was the most common signs in 25 cases (69.4%), followed by liver enlargement in 23 cases (63.9%). The levels of CK-MB were higher than normal in 17 cases and the levels of 13 patients' cTnI were increased; ECG with ectopic beat, ectopic rhythm and atrioventricular block were common; ECHO showed that all of the children's left ventricular end diastolic (LEVD) were expanded in varying degrees (100%) and 31 cases (86.1%) with the left ventricular ejection fraction (LVEF) of b0.5. Left ventricular mural thrombus was found in 1 case and pulmonary hypertension in 7 cases. All children were treated by diuretic, angiotensin-converting enzyme inhibitor (ACEI) and cardiotonics. 17 children with tachycardia and arrhythmia have also taken β-blocker, 4 cases complicated with atrial tachycardia and atrial fibrillation have taken amiodarone. 16 patients used intravenous immune globulin (IVIG) and 4 of them with severe complications used combined hormone therapy. 33 cases' clinical symptoms improved and 3 patients had bad outcomes. Conclusion: Pediatric dilated cardiomyopathy is common in infancy, pre-school age and school age. Most have been heart failure, cardiomegaly and tachyarrhythmia in different degrees when diagnosed. Combined treatment of β-blocker and amiodarone could give a better result. doi:10.1016/j.ijcard.2011.08.645 0377 Marital status in relation to case fatality after myocardial infarction — A population based study XIUYING ZHANGa, DONG ZHAOa, XUEQIN XIEb, ZAIHUA WEIc, WEI WANGa, JIAYI SUNa, MIAO WANGa, JUN LIUa, YUE QIa, MONING GUOb, JIANPENG ZHENGb, JING LIUa a Department of Epidemiology, Capital Medical University Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China b Beijing Public Health Information Center, Beijing, China c Beijing Centers for Diseases Control and Prevention, Beijing, China Objective: Marital status has been associated with mortality of acute coronary event, however, the relationship with case fatality rates (CFR) is unclear. To explore whether CFR differ between groups defined in terms of marital status. Methods: The Beijing Acute Myocardial Infarction Surveillance Platform was established by linking the routinely collected data from the Hospital Discharge Information System and Cause of Death Register System in Beijing. Patients who were hospitalized for myocardial infarction or died from acute myocardial infarction during Jan. 1, 2007, to Dec. 31, 2009 were extracted. Results: A total of 52,357 patients were identified among the permanent residences in Beijing and aged 25 years or more. The mean (±standard deviation) age was 68.4 (±13.4) years, and 33,100 (63.4%) were men. Unmarried status had higher CFR than married (36.2%, 64.9%, 92.1% and 33.7% for never married, divorced, widowed and married, respectively, P b 0.01). Similarly, unmarried patients were more likely to die out of hospital, compared to the married one. The proportion of death out of hospital was 80.8%, 81.2%, 90.9% and 67.4% for never married, divorced, widowed and married, respectively (P b 0.01). In-hospital case fatality was 5.1%, 10.7%, 11.3% and 6.8% for never married, divorced, widowed and married, respectively. After adjustments for age and sex, unmarried status was also related with an increased CFR (odds ratio (OR) 1.33, 95% CI: 1.19–1.50; 4.43:3.64–5.40 and14.26:13.02–15.62 for never married, divorced and widowed, respectively, compared to the married), but not for in-hospital case fatality. Conclusion: Unmarried status was significantly related to CFR. doi:10.1016/j.ijcard.2011.08.646
Abstracts
manner in beating rabbit atria. Conclusions: These findings suggest that cardiac CNP regulates NO production. doi:10.1016/j.ijcard.2011.08.643 0277 Xinshuitong capsule ameliorates hypertrophy of cardiomyocytes via aquaporin pathway in the ischemia–reperfusion rat hearts CHUNJIANG TAN, YANBIN WU, WENLIE CHEN, RUHUI LIN Fujian Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province, China Objective: Aquaporin-1 (AQP1), which was present in both human and rat hearts, mediated the water transport, however, Xinshuitong capsule (XC), an aquaretic herbal compound, whether affects AQP1 pathway remains unclear. Materials and methods: 20 male Sprague– Dawley rats (280 ± 83 g) underwent 30 min ischemia by ligation of anterior coronary artery followed by 2 h reperfusion. Animals were randomly treated with XC (0.5 g/kg i.p, during ischemia and reperfusion) or vehicle with 5 sham rats as controls. The animals were killed at 1, 2, 4, 8 and 16 h after surgery. In vitro, AQP1 gene in cardiomyocytes was knocked down or underwent hypoxia/reoxygenation exposed to XC (10, 20, 40 and 80 μg/mL). The ultrastructure of cardiomyocytes was observed by transmission electron microscope. AQP1 in mRNA and protein expressions were determined for all hearts by laser confocal fluorescence microscopy. Results: Vehicle rats showed a prominent cells edema, mitochondrial swelling associated with crest and membrane disruption with abundant lipid-like structures in the cytoplasm at 1–4 h, and a high incidence of plasma membrane rupture at 8–16 h. With an increase in cell volume, a higher fluorescence of AQP1 was detected in the ischemic center and the border zone than in the noninfarcted area. By contrast, XC can significantly ameliorate cells morphology in vivo and in vitro. Compared to the controls, the XC-treated cells showed more tolerant to hypoxic stress, which related to XC dosage in the hypoxia–reoxygenation experiments. XC can further significantly up-regulate AQP1 mRNA and its protein expressions in the XC-treated rat hearts. Identical results were also detected in the XC-treated hypoxia–reoxygenation cells with a marked increase in AQP1 mRNA and its protein than the control. Knocked down gene AQP1, XC presented little effect on the change in cell size. Conclusions: The data suggest that XC can preserve the profile of cardiomyocytes via up-regulation of AQP1 pathway in the ischemia–reperfusion rat hearts. doi:10.1016/j.ijcard.2011.08.644 0376 Clinical features and therapeutic strategy of 36 cases of dilated cardiomyopathy in children XI YANG, GUIYING LIU Beijing Anzhen Hospital, Beijing, China Objective: To explore the clinical features, treatment effect and prognosis of dilated cardiomyopathy (DCM) in children. Methods: Give a comprehensive analysis of 36 children with DCM in Beijing Anzhen Hospital, including clinical manifestations, biomarkers, electrocardiogram (ECG), X-ray, echocardiography (ECHO), drug selection, therapeutic effect and prognosis. Results: All patients have cardiomegaly and heart failure on admission. 11 cases of heart function II, 14 cases of heart function III and 8 cases of heart function IV. The common symptoms were edema in 16 cases (44.4%), anhelation in 15 cases (41.6%) and anorexia in 15 cases (41.6%). Initial symptoms manifested as palpitations in 7 cases (19.4%) and
respiratory infection in 7 cases (19.4%). Apical murmur was the most common signs in 25 cases (69.4%), followed by liver enlargement in 23 cases (63.9%). The levels of CK-MB were higher than normal in 17 cases and the levels of 13 patients' cTnI were increased; ECG with ectopic beat, ectopic rhythm and atrioventricular block were common; ECHO showed that all of the children's left ventricular end diastolic (LEVD) were expanded in varying degrees (100%) and 31 cases (86.1%) with the left ventricular ejection fraction (LVEF) of b0.5. Left ventricular mural thrombus was found in 1 case and pulmonary hypertension in 7 cases. All children were treated by diuretic, angiotensin-converting enzyme inhibitor (ACEI) and cardiotonics. 17 children with tachycardia and arrhythmia have also taken β-blocker, 4 cases complicated with atrial tachycardia and atrial fibrillation have taken amiodarone. 16 patients used intravenous immune globulin (IVIG) and 4 of them with severe complications used combined hormone therapy. 33 cases' clinical symptoms improved and 3 patients had bad outcomes. Conclusion: Pediatric dilated cardiomyopathy is common in infancy, pre-school age and school age. Most have been heart failure, cardiomegaly and tachyarrhythmia in different degrees when diagnosed. Combined treatment of β-blocker and amiodarone could give a better result. doi:10.1016/j.ijcard.2011.08.645 0377 Marital status in relation to case fatality after myocardial infarction — A population based study XIUYING ZHANGa, DONG ZHAOa, XUEQIN XIEb, ZAIHUA WEIc, WEI WANGa, JIAYI SUNa, MIAO WANGa, JUN LIUa, YUE QIa, MONING GUOb, JIANPENG ZHENGb, JING LIUa a Department of Epidemiology, Capital Medical University Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China b Beijing Public Health Information Center, Beijing, China c Beijing Centers for Diseases Control and Prevention, Beijing, China Objective: Marital status has been associated with mortality of acute coronary event, however, the relationship with case fatality rates (CFR) is unclear. To explore whether CFR differ between groups defined in terms of marital status. Methods: The Beijing Acute Myocardial Infarction Surveillance Platform was established by linking the routinely collected data from the Hospital Discharge Information System and Cause of Death Register System in Beijing. Patients who were hospitalized for myocardial infarction or died from acute myocardial infarction during Jan. 1, 2007, to Dec. 31, 2009 were extracted. Results: A total of 52,357 patients were identified among the permanent residences in Beijing and aged 25 years or more. The mean (±standard deviation) age was 68.4 (±13.4) years, and 33,100 (63.4%) were men. Unmarried status had higher CFR than married (36.2%, 64.9%, 92.1% and 33.7% for never married, divorced, widowed and married, respectively, P b 0.01). Similarly, unmarried patients were more likely to die out of hospital, compared to the married one. The proportion of death out of hospital was 80.8%, 81.2%, 90.9% and 67.4% for never married, divorced, widowed and married, respectively (P b 0.01). In-hospital case fatality was 5.1%, 10.7%, 11.3% and 6.8% for never married, divorced, widowed and married, respectively. After adjustments for age and sex, unmarried status was also related with an increased CFR (odds ratio (OR) 1.33, 95% CI: 1.19–1.50; 4.43:3.64–5.40 and14.26:13.02–15.62 for never married, divorced and widowed, respectively, compared to the married), but not for in-hospital case fatality. Conclusion: Unmarried status was significantly related to CFR. doi:10.1016/j.ijcard.2011.08.646