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120 Hours
CHAPTER
8
120 HOURS Sagittal Sections Figure 8.1
1
2
3
4
5
Figure 8.2
1
2
3
4 7
5
6
Figure 8.3
2
8
3
7
5
6
1. lens 2. optic tectum 3. semicircular canal 4. pectoral fin 5. opercle 6. pectoral fin musculature 7. opercular cavity 8. torus lateralis
Atlas of Zebrafish Development. 10.1016/B978-0-12-374016-8.00008-X © 2012 Elsevier Inc. All rights reserved.
101
102
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.4
1
2
3
7
6
4
5
Figure 8.5
8
1
3
6
7
5
Figure 8.6
9
10
8
1
12
6
1. optic tectum 2. torus lateralis
3. granular eminence 4. semicircular canal
11
5
5. pectoral fin musculature 6. gill filaments
7. opercular cavity 8. inferior lobe
9. olfactory pit 10. telencephalon
11. cerebellum 12. swim bladder
120 hours
103
Figure 8.7
2
1
4
3
5
6
8
9
7
Figure 8.8
2
6
4 5
3
7
9
10
11
Figure 8.9
2
3
13
1. olfactory pit 2. telencephalon 3. optic tectum
4. inferior lobe 5. cerebellum 6. swim bladder
6
4 5
7
12
10
11
7. liver 8. pectoral fin musculature 9. gill filaments
10. myotome 11. intestinal bulb 12. vertical myoseptum
13. sternohyoid
104
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.10
3 1
2
5
7
6
4
8
9
14
13 12
10
11
Figure 8.11
1
2
5
3 4
15
16
8
9 14
19
17 12 18
6
7
10
11
Figure 8.12
1
2
5
3 4
15
8
9
19 18
1. 2. 3. 4.
telencephalon optic tectum inferior lobe cerebellum
5. 6. 7. 8.
swim bladder vertical myoseptum myotome posterior intestine
17 12
16
7
10
11
9. 10. 11. 12.
mid intestine intestinal bulb liver esophagus
13. 14. 15. 16.
cardiac ventricle sternohyoid spinal cord notochord
17. atrium 18. cardiac ventricle 19. bulbus arteriosus
120 hours
105
Figure 8.13
1
5
3 4
2
7
6
9
10 17
16
8
11
12 14 13 15
Figure 8.14
1
2
5
3 4
9
10 17
16
12
11
19
5
8
7
15 18 13 14
Figure 8.15
1
2
15
1. 2. 3. 4.
telencephalon optic tectum inferior lobe cerebellum
5. 6. 7. 8.
swim bladder spinal cord myotome notochord
3 4
17 18
12
9. 10. 11. 12.
7
8
11
posterior intestine mid intestine intestinal bulb liver
13. 14. 15. 16.
esophagus atrium opercular cavity sternohyoid
17. gill filaments 18. pectoral fin musculature 19. pronephros
106
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.16
2
1
13
11 12
10
8
6
5
3 4
7
9
Figure 8.17
2
1
7
3 4
13
6
11 12
10
9
Figure 8.18
2
13
1. 2. 3. 4.
olfactory pit optic tectum inferior lobe cerebellum
5. 6. 7. 8.
3 14 15
16
12 11
pronephros swim bladder myotome vertebral column
9. 10. 11. 12.
intestinal bulb liver pectoral fin musculature gill filaments
13. 14. 15. 16.
opercular cavity granular eminence semicircular canal opercle
120 hours
107
Figure 8.19
1
2
3
6
5
4
Figure 8.20
3
1
4
5 7
6
Figure 8.21
3
1
8 7
1. optic tectum 2. torus lateralis
4
3. semicircular canal 4. pectoral fin musculature
5. opercular cavity 6. gill filaments
7. opercle 8. pectoral fin
108
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.22
1
3
2
4 5
6
Figure 8.23
1
3
4
6
Figure 8.24
1
3
4
1. lens
2. optic tectum
3. semicircular canal
4. pectoral fin
5. pectoral fin musculature
6. opercle
120 hours
109
Coronal Sections Figure 8.25
2
1
3
Figure 8.26
1
4
3
2
Figure 8.27
5
6
1
3
4
1. medulla oblongata
2. spinal cord
3. myotome
4. notochord
5. optic tectum
6. cerebellum
110
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 8.28
2
1
3
4
5
6
Figure 8.29
1
2
3
4
5
6 7
Figure 8.30
1 8
2
3
10
1. telencephalon 2. optic tectum
3. cerebellum 4. medulla oblongata
4
5
9
6
7
5. myotome 6. notochord
7. semicircular canal 8. olfactory pit
9. swim bladder 10. olfactory epithelium
120 hours
111
Figure 8.31
2
3
1
5
4
6
8
11
10
7
9
Figure 8.32
2 3
4
5
12
6
8
11
6
7
9
Figure 8.33
2
3
5
12
6 6
8
7
9
1. olfactory pit 2. telencephalon
3. optic tectum 4. cerebellum
5. medulla oblongata 6. myotome
7. swim bladder 8. notochord
9. semicircular canal 10. tectal ventricle
11. olfactory epithelium 12. dorsal mandibular arch
112
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.34
2
3
4
1
5 5
6
7 8 9
Figure 8.35
2
3
10
1
11
12
13
6 8 9
Figure 8.36
3
14
1
11
17
dorsal mandibular arch telencephalon optic tectum medulla oblongata
13
15
6 9
1. 2. 3. 4.
12
5. 6. 7. 8.
16
8
myotome swim bladder notochord semicircular canal
9. 10. 11. 12.
lens granular eminence pancreas mid intestine
13. 14. 15. 16.
posterior intestine pectoral fin median fin fold hypothalamus
17. ventral mandibular arch
120 hours
113
Figure 8.37
2
3 4
1
5
8 11
6
7
9
10
Figure 8.38
2
3 4
5 6
12
8
7
9 11
Figure 8.39
13
3 4 5
12
7 14
1. dorsal mandibular arch 2. optic tectum 3. pectoral fin
6
9
4. pancreas 5. mid intestine 6. median fin fold
8
7. swim bladder 8. liver 9. semicircular canal
10. hypothalamus 11. lens 12. ventral mandibular arch
13. otolith 14. inferior lobe
114
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.40
1
2
3
4 5
9
6 7
8
Figure 8.41
1
2 3
10
4 5
9 7
8
Figure 8.42
1
11
2
3
4
9 13
1. hyomandibula 2. pectoral fin 3. pancreas
4. intestinal bulb 5. median fin fold 6. swim bladder
12
7. liver 8. inferior lobe 9. ventral mandibular arch
10. intermandibularis 11. opercle 12. esophagus
13. opercular cavity
120 hours
115
Figure 8.43
1
3
2
8
4
5
6
7
Figure 8.44
1
3
2
4 5
8 7
9
Figure 8.45
3
2
5
8 7
1. hyomandibula 2. opercle
3. pectoral fin 4. pancreas
9
5. intestinal bulb 6. esophagus
7. opercular cavity 8. ventral mandibular arch
9. liver
116
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.46
1
2 3
6
4
5
Figure 8.47
2
1
7
3
6
4
5
8
Figure 8.48
9
2
7
3
8
1. opercle 2. pectoral fin
3. intestinal bulb 4. liver
10
4
5. opercular cavity 6. adductor mandibulae complex
7. ceratohyal 8. gill filaments
9. cardiac ventricle 10. atrium
120 hours
117
Figure 8.49
2
1
3
5
6
7
4
Figure 8.50
1
2
3
4
7
Figure 8.51
3
2
7
1. ceratohyal
2. cardiac ventricle
4
3. intestinal bulb
4. liver
5. atrium
6. bulbus arteriosus
7. sternohyoid
118
ATLAS OF ZEBRAFISH DEVELOPMENT
Transverse Sections Figure 8.52
Figure 8.53
2
2 1
3 1
Figure 8.54
Figure 8.55 5
5
6 4
4 1
Figure 8.56
Figure 8.57 9 9
10
7
4
4 8
8
1. ventral mandibular arch 2. dorsal mandibular arch
3. intermandibularis 4. optic chiasm
5. telencephalon 6. lens
7. hypothalamus 8. gill filaments
9. optic tectum 10. inferior lobe
120 hours Figure 8.58
119
Figure 8.59
12
7
8 1 6
11 10
2
5
3
6
2 9
4
Figure 8.60
Figure 8.61
8
8 11
11 10
13
15 13
14
9
Figure 8.62
Figure 8.63
20
8 19
16
19
16
21 14
18
18
14
22
17 17
1. 2. 3. 4. 5.
inferior lobe opercle sternohyoid bulbus arteriosus gill filaments
6. 7. 8. 9. 10.
opercular cavity optic tectum medulla oblongata cardiac ventricle pharynx
11. 12. 13. 14. 15.
semicircular canal cerebellum atrium liver pectoral fin musculature
16. 17. 18. 19. 20.
myotome intestinal bulb pectoral fin notochord spinal cord
21. swim bladder 22. pancreas
120
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.64
Figure 8.65
1
1 2
7
2
7
3
3
6
5
5
4
4
Figure 8.66
Figure 8.67
1
1 7
7
2 3
2
5
8
5 8
Figure 8.68
Figure 8.69
1 7
1 2
5
7
2 8
8 9
9
1. spinal cord 2. myotome 3. swim bladder 4. intestinal bulb 5. pancreas 6. pectoral fin 7. notochord 8. mid intestine 9. median fin fold
120 hours Figure 8.70
121
Figure 8.71
1 5
1 5
2
2
3
6
4
4
Figure 8.72
Figure 8.73 4 1 5
1 5
2
2 6
6 4
Figure 8.74
Figure 8.75 4
4
1
1 5
5
2
6
6
1. spinal cord
2. myotome
3. mid intestine
2
4. median fin fold
5. notochord
6. posterior intestine
8
120 HOURS Sagittal Sections Figure 8.1
1
2
3
4
5
Figure 8.2
1
2
3
4 7
5
6
Figure 8.3
2
8
3
7
5
6
1. lens 2. optic tectum 3. semicircular canal 4. pectoral fin 5. opercle 6. pectoral fin musculature 7. opercular cavity 8. torus lateralis
Atlas of Zebrafish Development. 10.1016/B978-0-12-374016-8.00008-X © 2012 Elsevier Inc. All rights reserved.
101
102
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.4
1
2
3
7
6
4
5
Figure 8.5
8
1
3
6
7
5
Figure 8.6
9
10
8
1
12
6
1. optic tectum 2. torus lateralis
3. granular eminence 4. semicircular canal
11
5
5. pectoral fin musculature 6. gill filaments
7. opercular cavity 8. inferior lobe
9. olfactory pit 10. telencephalon
11. cerebellum 12. swim bladder
120 hours
103
Figure 8.7
2
1
4
3
5
6
8
9
7
Figure 8.8
2
6
4 5
3
7
9
10
11
Figure 8.9
2
3
13
1. olfactory pit 2. telencephalon 3. optic tectum
4. inferior lobe 5. cerebellum 6. swim bladder
6
4 5
7
12
10
11
7. liver 8. pectoral fin musculature 9. gill filaments
10. myotome 11. intestinal bulb 12. vertical myoseptum
13. sternohyoid
104
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.10
3 1
2
5
7
6
4
8
9
14
13 12
10
11
Figure 8.11
1
2
5
3 4
15
16
8
9 14
19
17 12 18
6
7
10
11
Figure 8.12
1
2
5
3 4
15
8
9
19 18
1. 2. 3. 4.
telencephalon optic tectum inferior lobe cerebellum
5. 6. 7. 8.
swim bladder vertical myoseptum myotome posterior intestine
17 12
16
7
10
11
9. 10. 11. 12.
mid intestine intestinal bulb liver esophagus
13. 14. 15. 16.
cardiac ventricle sternohyoid spinal cord notochord
17. atrium 18. cardiac ventricle 19. bulbus arteriosus
120 hours
105
Figure 8.13
1
5
3 4
2
7
6
9
10 17
16
8
11
12 14 13 15
Figure 8.14
1
2
5
3 4
9
10 17
16
12
11
19
5
8
7
15 18 13 14
Figure 8.15
1
2
15
1. 2. 3. 4.
telencephalon optic tectum inferior lobe cerebellum
5. 6. 7. 8.
swim bladder spinal cord myotome notochord
3 4
17 18
12
9. 10. 11. 12.
7
8
11
posterior intestine mid intestine intestinal bulb liver
13. 14. 15. 16.
esophagus atrium opercular cavity sternohyoid
17. gill filaments 18. pectoral fin musculature 19. pronephros
106
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.16
2
1
13
11 12
10
8
6
5
3 4
7
9
Figure 8.17
2
1
7
3 4
13
6
11 12
10
9
Figure 8.18
2
13
1. 2. 3. 4.
olfactory pit optic tectum inferior lobe cerebellum
5. 6. 7. 8.
3 14 15
16
12 11
pronephros swim bladder myotome vertebral column
9. 10. 11. 12.
intestinal bulb liver pectoral fin musculature gill filaments
13. 14. 15. 16.
opercular cavity granular eminence semicircular canal opercle
120 hours
107
Figure 8.19
1
2
3
6
5
4
Figure 8.20
3
1
4
5 7
6
Figure 8.21
3
1
8 7
1. optic tectum 2. torus lateralis
4
3. semicircular canal 4. pectoral fin musculature
5. opercular cavity 6. gill filaments
7. opercle 8. pectoral fin
108
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.22
1
3
2
4 5
6
Figure 8.23
1
3
4
6
Figure 8.24
1
3
4
1. lens
2. optic tectum
3. semicircular canal
4. pectoral fin
5. pectoral fin musculature
6. opercle
120 hours
109
Coronal Sections Figure 8.25
2
1
3
Figure 8.26
1
4
3
2
Figure 8.27
5
6
1
3
4
1. medulla oblongata
2. spinal cord
3. myotome
4. notochord
5. optic tectum
6. cerebellum
110
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 8.28
2
1
3
4
5
6
Figure 8.29
1
2
3
4
5
6 7
Figure 8.30
1 8
2
3
10
1. telencephalon 2. optic tectum
3. cerebellum 4. medulla oblongata
4
5
9
6
7
5. myotome 6. notochord
7. semicircular canal 8. olfactory pit
9. swim bladder 10. olfactory epithelium
120 hours
111
Figure 8.31
2
3
1
5
4
6
8
11
10
7
9
Figure 8.32
2 3
4
5
12
6
8
11
6
7
9
Figure 8.33
2
3
5
12
6 6
8
7
9
1. olfactory pit 2. telencephalon
3. optic tectum 4. cerebellum
5. medulla oblongata 6. myotome
7. swim bladder 8. notochord
9. semicircular canal 10. tectal ventricle
11. olfactory epithelium 12. dorsal mandibular arch
112
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.34
2
3
4
1
5 5
6
7 8 9
Figure 8.35
2
3
10
1
11
12
13
6 8 9
Figure 8.36
3
14
1
11
17
dorsal mandibular arch telencephalon optic tectum medulla oblongata
13
15
6 9
1. 2. 3. 4.
12
5. 6. 7. 8.
16
8
myotome swim bladder notochord semicircular canal
9. 10. 11. 12.
lens granular eminence pancreas mid intestine
13. 14. 15. 16.
posterior intestine pectoral fin median fin fold hypothalamus
17. ventral mandibular arch
120 hours
113
Figure 8.37
2
3 4
1
5
8 11
6
7
9
10
Figure 8.38
2
3 4
5 6
12
8
7
9 11
Figure 8.39
13
3 4 5
12
7 14
1. dorsal mandibular arch 2. optic tectum 3. pectoral fin
6
9
4. pancreas 5. mid intestine 6. median fin fold
8
7. swim bladder 8. liver 9. semicircular canal
10. hypothalamus 11. lens 12. ventral mandibular arch
13. otolith 14. inferior lobe
114
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.40
1
2
3
4 5
9
6 7
8
Figure 8.41
1
2 3
10
4 5
9 7
8
Figure 8.42
1
11
2
3
4
9 13
1. hyomandibula 2. pectoral fin 3. pancreas
4. intestinal bulb 5. median fin fold 6. swim bladder
12
7. liver 8. inferior lobe 9. ventral mandibular arch
10. intermandibularis 11. opercle 12. esophagus
13. opercular cavity
120 hours
115
Figure 8.43
1
3
2
8
4
5
6
7
Figure 8.44
1
3
2
4 5
8 7
9
Figure 8.45
3
2
5
8 7
1. hyomandibula 2. opercle
3. pectoral fin 4. pancreas
9
5. intestinal bulb 6. esophagus
7. opercular cavity 8. ventral mandibular arch
9. liver
116
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.46
1
2 3
6
4
5
Figure 8.47
2
1
7
3
6
4
5
8
Figure 8.48
9
2
7
3
8
1. opercle 2. pectoral fin
3. intestinal bulb 4. liver
10
4
5. opercular cavity 6. adductor mandibulae complex
7. ceratohyal 8. gill filaments
9. cardiac ventricle 10. atrium
120 hours
117
Figure 8.49
2
1
3
5
6
7
4
Figure 8.50
1
2
3
4
7
Figure 8.51
3
2
7
1. ceratohyal
2. cardiac ventricle
4
3. intestinal bulb
4. liver
5. atrium
6. bulbus arteriosus
7. sternohyoid
118
ATLAS OF ZEBRAFISH DEVELOPMENT
Transverse Sections Figure 8.52
Figure 8.53
2
2 1
3 1
Figure 8.54
Figure 8.55 5
5
6 4
4 1
Figure 8.56
Figure 8.57 9 9
10
7
4
4 8
8
1. ventral mandibular arch 2. dorsal mandibular arch
3. intermandibularis 4. optic chiasm
5. telencephalon 6. lens
7. hypothalamus 8. gill filaments
9. optic tectum 10. inferior lobe
120 hours Figure 8.58
119
Figure 8.59
12
7
8 1 6
11 10
2
5
3
6
2 9
4
Figure 8.60
Figure 8.61
8
8 11
11 10
13
15 13
14
9
Figure 8.62
Figure 8.63
20
8 19
16
19
16
21 14
18
18
14
22
17 17
1. 2. 3. 4. 5.
inferior lobe opercle sternohyoid bulbus arteriosus gill filaments
6. 7. 8. 9. 10.
opercular cavity optic tectum medulla oblongata cardiac ventricle pharynx
11. 12. 13. 14. 15.
semicircular canal cerebellum atrium liver pectoral fin musculature
16. 17. 18. 19. 20.
myotome intestinal bulb pectoral fin notochord spinal cord
21. swim bladder 22. pancreas
120
ATLAS OF ZEBRAFISH DEVELOPMENT Figure 8.64
Figure 8.65
1
1 2
7
2
7
3
3
6
5
5
4
4
Figure 8.66
Figure 8.67
1
1 7
7
2 3
2
5
8
5 8
Figure 8.68
Figure 8.69
1 7
1 2
5
7
2 8
8 9
9
1. spinal cord 2. myotome 3. swim bladder 4. intestinal bulb 5. pancreas 6. pectoral fin 7. notochord 8. mid intestine 9. median fin fold
120 hours Figure 8.70
121
Figure 8.71
1 5
1 5
2
2
3
6
4
4
Figure 8.72
Figure 8.73 4 1 5
1 5
2
2 6
6 4
Figure 8.74
Figure 8.75 4
4
1
1 5
5
2
6
6
1. spinal cord
2. myotome
3. mid intestine
2
4. median fin fold
5. notochord
6. posterior intestine