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372 Estrogen (E), progestin (P) and androgen (A) receptors (R) in human intracranial glioma and meningioma
124s
371
S-104
01 The
Stockholm, glucocorticoid
Sweden. receptor
proteins
in
cytosol
from
human
and
rat
brain
are
simi-
lar both with regard to protein structure and hormone specificity. The average concentration of glucocorticoid receptor in various intracranial lesions was as follows: cerebral metastases > meningiomas > acoustic neuromas > gliomas > peritumoral brain tissue > contused brain tissue. The clinical response to dexamethasone in brain edema associated with various intracranial lesions is to some extent parallel to the glucocorticoid receptor concentration. The study on the relationship between computed tomography (CT) findings and in various intracranial tumors showed glucocorticoid receptor concentrations that some correlation existed between the glucocorticoid receptor level and CT contrast enhancement. This indicated that glucocorticoid receptor interaction is of importance in the regulation of the vascular permeability of tumors. The effect of dexamethasone treatment on brain edema was evaluated by CT in The degree of reduction of edema 14 patients with various intracranial tumors. volume (cerebra1 paralleled the
372
metastases > gliomas > meningiomas) clinical response of dexamethasone.
following
steroid
treatment
ESTROGEN (E), PROGESTIN (P) AND ANDROGEN (A) RECEPTORS (R) IN HUMAN INTRACRANIAL GLIOMA AND MENINGIOMA. B.F. Pertuiset (1) M. Moguilewsky (2) and M. Poisson (1). (1)Dept. Neurology, La Pitie-Salpdtriere Faculty of Medicine, Paris, (2) Roussel UCLAF Research Centre, Romainville, France. Recently, interest has centered on sex steroid receptors, i.e. ER, PR, and AR, in intracranial tumors because more specific treatments of patients with malignant glioma and recurrent and/or unoperable meningioma are needed, and because epidemiological and clinical data have indicated some sex hormone sensitivity. We therefore assayed adult tumor biopsy specimens systematically for cytosolic receptors, i.e. ERc,PRc and ARC, using respectively (3H) R2858, R5020 (or RU27987) and R1881 as radioligands, and in some cases for nuclear receptors (Rn). R5020 binding sites were also measured in meningioma cell cultures. In glioma (n = 20), ARC (mean Kd: 2nM) and ARn were detected, but at low levels, in 90 and 60% of samples respectively; ERc was detected in a few cases only and PRc was apparently absent. In meningioma (n = 46), ERc, PRc and ARC were found in 33, 93 and 87% of samples respectively (Kd of the nM order). ER levels were low in the cytosol and/or the nucleus whereas ARC and PRc (and PRn) levels were moderate to high (PR levels up to 25000 fmol/gT) enabling biochemical characterization of AR and PR. R5020 binding activity was detectable in meningioma cell cultures. The relevance of AR in glioma and of AR and PR in meningioma is discussed in relation to tumor cell proliferation and differentiation.
373
HORMONE RECEPTORS IN BRAIN TUMORS Roberta Glick, M.D., Division of Neurosurgery; Agostino Molteni, M.D.,PhD., Dept. of Pathology; Ellen Flors, M.S., Dept. of Pathology; Anthony 3. Raimondi, M.D., Division of Neurosurgery; Northwestern Univ. Medical School, Chicago, Illinois, U.S.A. The presence of hormone receptors in a variety of tumors outside the nervous system has been found to correlate with their degree of malignancy and has prognostic In this study, brain tumors from 64 value for their response to endocrine therapy. This patients were studied for the presence of estradiol and progestin receptors. study included malignant and benign tumors, in male and female patients, in pre- and Estradiol receptors were found post-menopausal females, and in adults and children. in 34 of the 64 patients: 13/21 meningiomas, 4/8 Schwannomas, l/5 benign gliomas, 4/5 malignant gliomas, 1 oligodendroglioma, 5/11 metastases, and 6/11 pediatric tumors. Eleven patients were studied for progesterone receptors and were measureable in 7. Of the 34 tumors with measureable estradiol receptors, 23 occurred in females, and 4 of 5 of th 6 pediatric tumors the 7 patients with progesterone receptors were female. with receptors were from malignant tumors and the only pediatric tumors with progesterOf the 10 gliomas studied, 4/S malignant tumors had one receptors were also malignant. estradiol receptors, whereas only l/5 benign tumors showed binding, and this was Our studies with the pediatric tumors and the gliomas sugstatistically significant. gest that a relationship exits between the malignancy of the tumor and the presence of hormone receptors.
371
S-104
01 The
Stockholm, glucocorticoid
Sweden. receptor
proteins
in
cytosol
from
human
and
rat
brain
are
simi-
lar both with regard to protein structure and hormone specificity. The average concentration of glucocorticoid receptor in various intracranial lesions was as follows: cerebral metastases > meningiomas > acoustic neuromas > gliomas > peritumoral brain tissue > contused brain tissue. The clinical response to dexamethasone in brain edema associated with various intracranial lesions is to some extent parallel to the glucocorticoid receptor concentration. The study on the relationship between computed tomography (CT) findings and in various intracranial tumors showed glucocorticoid receptor concentrations that some correlation existed between the glucocorticoid receptor level and CT contrast enhancement. This indicated that glucocorticoid receptor interaction is of importance in the regulation of the vascular permeability of tumors. The effect of dexamethasone treatment on brain edema was evaluated by CT in The degree of reduction of edema 14 patients with various intracranial tumors. volume (cerebra1 paralleled the
372
metastases > gliomas > meningiomas) clinical response of dexamethasone.
following
steroid
treatment
ESTROGEN (E), PROGESTIN (P) AND ANDROGEN (A) RECEPTORS (R) IN HUMAN INTRACRANIAL GLIOMA AND MENINGIOMA. B.F. Pertuiset (1) M. Moguilewsky (2) and M. Poisson (1). (1)Dept. Neurology, La Pitie-Salpdtriere Faculty of Medicine, Paris, (2) Roussel UCLAF Research Centre, Romainville, France. Recently, interest has centered on sex steroid receptors, i.e. ER, PR, and AR, in intracranial tumors because more specific treatments of patients with malignant glioma and recurrent and/or unoperable meningioma are needed, and because epidemiological and clinical data have indicated some sex hormone sensitivity. We therefore assayed adult tumor biopsy specimens systematically for cytosolic receptors, i.e. ERc,PRc and ARC, using respectively (3H) R2858, R5020 (or RU27987) and R1881 as radioligands, and in some cases for nuclear receptors (Rn). R5020 binding sites were also measured in meningioma cell cultures. In glioma (n = 20), ARC (mean Kd: 2nM) and ARn were detected, but at low levels, in 90 and 60% of samples respectively; ERc was detected in a few cases only and PRc was apparently absent. In meningioma (n = 46), ERc, PRc and ARC were found in 33, 93 and 87% of samples respectively (Kd of the nM order). ER levels were low in the cytosol and/or the nucleus whereas ARC and PRc (and PRn) levels were moderate to high (PR levels up to 25000 fmol/gT) enabling biochemical characterization of AR and PR. R5020 binding activity was detectable in meningioma cell cultures. The relevance of AR in glioma and of AR and PR in meningioma is discussed in relation to tumor cell proliferation and differentiation.
373
HORMONE RECEPTORS IN BRAIN TUMORS Roberta Glick, M.D., Division of Neurosurgery; Agostino Molteni, M.D.,PhD., Dept. of Pathology; Ellen Flors, M.S., Dept. of Pathology; Anthony 3. Raimondi, M.D., Division of Neurosurgery; Northwestern Univ. Medical School, Chicago, Illinois, U.S.A. The presence of hormone receptors in a variety of tumors outside the nervous system has been found to correlate with their degree of malignancy and has prognostic In this study, brain tumors from 64 value for their response to endocrine therapy. This patients were studied for the presence of estradiol and progestin receptors. study included malignant and benign tumors, in male and female patients, in pre- and Estradiol receptors were found post-menopausal females, and in adults and children. in 34 of the 64 patients: 13/21 meningiomas, 4/8 Schwannomas, l/5 benign gliomas, 4/5 malignant gliomas, 1 oligodendroglioma, 5/11 metastases, and 6/11 pediatric tumors. Eleven patients were studied for progesterone receptors and were measureable in 7. Of the 34 tumors with measureable estradiol receptors, 23 occurred in females, and 4 of 5 of th 6 pediatric tumors the 7 patients with progesterone receptors were female. with receptors were from malignant tumors and the only pediatric tumors with progesterOf the 10 gliomas studied, 4/S malignant tumors had one receptors were also malignant. estradiol receptors, whereas only l/5 benign tumors showed binding, and this was Our studies with the pediatric tumors and the gliomas sugstatistically significant. gest that a relationship exits between the malignancy of the tumor and the presence of hormone receptors.