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4634666 Human-murine hybridoma fusion partner
185
PATENT ABSTRACTS
4634665 PROCESSES FOR INSERTING DNA INTO EUCARYOTIC CELLS AND FOR PRODUCING PROTEINACEOUS MATERIALS Richard Axel, Michael H Wigler, Saul J Silverstein assigned to The Trustees of Columbia University in the City of New York The present invention relates to processes for inserting DNA into eucaryotic cells, particularly DNA which includes a gene or genes coding for desired proteinaceous materials for which no selective criteria exist. The insertion of such DNA molecules is accomplished by cotransforruing eucaryotic cells with such DNA together with a second DNA which corresponds to a gene coding for a selectable marker. This invention also concerns processes for producing proteinaceous materials such as insulin, interferon protein, growth hormone and the like which involve cotransforming eucaryotic cells with DNA which codes for these proteinaceous materials, growing the contransformed cells for production of the proteinaceous material and recovering the proteinaceous material so produced. The invention further relates to processes for inserting into eucaryotic cells a multiplicity of DNA molecules which includes genes coding for desired proteinaceous materials. The insertion of multiple copies of desired genes is accomplished by cotransformation with the desired genes and with amplifiable genes for a dominant selectable marker in the presence of successively higher amounts of an inhibitor. Alternatively, the insertion of multiple copies of desired genes is accomplished by transformation using DNA molecules formed by ligating a DNA molecule including the desired gene to a DNA molecule which includes an amplifiable gene coding for a dominant selectable phenotype such as a gene associated with resistance to a drug in the presence of successively higher amounts of an agent such as a drug against which the gene confers resistance so that only those eucaryotic cells into which multiple copies of the amplifiable gene have been inserted survive. Eucaryotic cells into which multiple copies of the amplifiable gene have been inserted additionally include multiple copies of the desired gene and may be used to produce multiple copies of proteinaceous molecules, In this way otherwise rare proteinaceous materials may be obtained in higher concentrations than are obtainable using conventional techniques. 4634666 HUMAN-MURINE HYBRIDOMA FUSION PARTNER
Edgar Engleman. Steven K H Foung. F Carl Grumet assigned to The Board of Trustees of the Leland Stanford Junior University By careful screening and mutation, a humanmurine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. The trioma fusion products of this immortalizing partner are stable producers of human monoclonal antibodies. A trioma which produces monoclonal human antivaricella zoster is disclosed. 4634678 PLASMID CLONING AND EXPRESSION VECTORS FOR USE IN MICROORGANISMS John S Salstrom, Dawn Newman, Douglas F Harbrecht, Shiu-Lok Hu assigned to Molecular Genetics Research and Development Limited Partnership DNA cloning and expression vectors capable of replication in a microbial host comprising from upstream to downstream (a) at least one promoter; (b) a translation start codon; (c) a cloning segment which provides a means for inserting nucleic acid sequences and (d) a sequence coding for a detectable gene product, out of translational phase with the translation start codon but capable of being readjusted to the translational phase of said start codon by insertion into the cloning segment of nucleic acid sequences containing the proper number of nucleotides for readjustment, said gene product providing a means for detecting expression of inserted nucleic acid sequences.
4634682
RADIOIMMUNOASSAYS FOR THE SERUM
THYMIC (rrs)
FACTOR
Bruce W Erickson. Kam-Fook Fok, Genevieve S Incefy, Kazuhiro Ohga assigned to SloanKettering Institute for Cancer Radioimmunossays for the quantitation of serum thymic factor (FTS), a thymic peptide hormone, are disclosed. Each assay employs an antibody specific for FTS, the monoclonal antibody or the antibody from the antiserum of a host animal; synthetic FTS or FTS analogue as the hormone standard; and a radiolabeled FTS analogue as the tracer. Also disclosed is a method of treating serum or other biological fluid prior to assay of FTS to remove interfering substances.
PATENT ABSTRACTS
4634665 PROCESSES FOR INSERTING DNA INTO EUCARYOTIC CELLS AND FOR PRODUCING PROTEINACEOUS MATERIALS Richard Axel, Michael H Wigler, Saul J Silverstein assigned to The Trustees of Columbia University in the City of New York The present invention relates to processes for inserting DNA into eucaryotic cells, particularly DNA which includes a gene or genes coding for desired proteinaceous materials for which no selective criteria exist. The insertion of such DNA molecules is accomplished by cotransforruing eucaryotic cells with such DNA together with a second DNA which corresponds to a gene coding for a selectable marker. This invention also concerns processes for producing proteinaceous materials such as insulin, interferon protein, growth hormone and the like which involve cotransforming eucaryotic cells with DNA which codes for these proteinaceous materials, growing the contransformed cells for production of the proteinaceous material and recovering the proteinaceous material so produced. The invention further relates to processes for inserting into eucaryotic cells a multiplicity of DNA molecules which includes genes coding for desired proteinaceous materials. The insertion of multiple copies of desired genes is accomplished by cotransformation with the desired genes and with amplifiable genes for a dominant selectable marker in the presence of successively higher amounts of an inhibitor. Alternatively, the insertion of multiple copies of desired genes is accomplished by transformation using DNA molecules formed by ligating a DNA molecule including the desired gene to a DNA molecule which includes an amplifiable gene coding for a dominant selectable phenotype such as a gene associated with resistance to a drug in the presence of successively higher amounts of an agent such as a drug against which the gene confers resistance so that only those eucaryotic cells into which multiple copies of the amplifiable gene have been inserted survive. Eucaryotic cells into which multiple copies of the amplifiable gene have been inserted additionally include multiple copies of the desired gene and may be used to produce multiple copies of proteinaceous molecules, In this way otherwise rare proteinaceous materials may be obtained in higher concentrations than are obtainable using conventional techniques. 4634666 HUMAN-MURINE HYBRIDOMA FUSION PARTNER
Edgar Engleman. Steven K H Foung. F Carl Grumet assigned to The Board of Trustees of the Leland Stanford Junior University By careful screening and mutation, a humanmurine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. The trioma fusion products of this immortalizing partner are stable producers of human monoclonal antibodies. A trioma which produces monoclonal human antivaricella zoster is disclosed. 4634678 PLASMID CLONING AND EXPRESSION VECTORS FOR USE IN MICROORGANISMS John S Salstrom, Dawn Newman, Douglas F Harbrecht, Shiu-Lok Hu assigned to Molecular Genetics Research and Development Limited Partnership DNA cloning and expression vectors capable of replication in a microbial host comprising from upstream to downstream (a) at least one promoter; (b) a translation start codon; (c) a cloning segment which provides a means for inserting nucleic acid sequences and (d) a sequence coding for a detectable gene product, out of translational phase with the translation start codon but capable of being readjusted to the translational phase of said start codon by insertion into the cloning segment of nucleic acid sequences containing the proper number of nucleotides for readjustment, said gene product providing a means for detecting expression of inserted nucleic acid sequences.
4634682
RADIOIMMUNOASSAYS FOR THE SERUM
THYMIC (rrs)
FACTOR
Bruce W Erickson. Kam-Fook Fok, Genevieve S Incefy, Kazuhiro Ohga assigned to SloanKettering Institute for Cancer Radioimmunossays for the quantitation of serum thymic factor (FTS), a thymic peptide hormone, are disclosed. Each assay employs an antibody specific for FTS, the monoclonal antibody or the antibody from the antiserum of a host animal; synthetic FTS or FTS analogue as the hormone standard; and a radiolabeled FTS analogue as the tracer. Also disclosed is a method of treating serum or other biological fluid prior to assay of FTS to remove interfering substances.