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5110920 HLA Typing method and DNA probes used therein
ii ‘hnsfus. Sci. Vol. 14. No. 1
5110920
HLA TYPING METHOD AND DNA PROBES USED THEREIN Henry A Erlich assigned to Cetus Corporation HLA typing based on restriction length polymorphism is carried out by: digesting an individual’s DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled DNA hybridization probe that hybridizes to an HLA DNA sequence involved in the polymorphism; and comparing the resulting genomic blotting pattern with a standard. This technique may be adapted to make paternity or transplant or transfusion compatibility determinations or to make disease association correlations to diagnose diseases or predict susceptibility to diseases. Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci, for use in the typing procedure are described.
33924 APPARATUS AND METHOD FOR STORING AND PROCESSING BLOOD Cesare R Valeri assigned to Autologous Carp
Blood
This disclosure relates to a blood processing and preservation system. Apparatus of the system comprises a primary collection bag, a blood collection tube connected to the bag and adapted to draw a unit of blood from a person and feed the blood into the bag, and a harness connected to the bag for conducting solutions and blood components into and out of the bag. The harness includes a first tube for removing at least one blood component from the bag while retaining another component, a second tube for moving a cryoprotective solution into and out of the bag, a third tube for conducting a wash solution into and out of the bag, and identification on the bag. The foregoing apparatus is usable in a blood system including the method steps of collecting a unit of blood from a person and delivering the blood to the collection bag, processing the blood to separate the components and removing the components except for the red blood cells from the bag, preparing the red blood cells for freezing by conducting a cryoprotective solution into the
bag and mixing this solution with the red blood cells, preserving the red blood cells by freezing, subsequently washing the red blood cells by conducting a wash solution and the cells into and out of the bag, and delivering the cells in the same collection bag to a recipient. The foregoing method may be used in a homologous blood system where the recipient is not the donor, or in an autologous blood system where the blood is reinfused in the person who furnished the blood, and the identification on the bag assures the recipient that he/she is receiving his/her own blood. Other blood components, such as the plasma, may also be preserved by freezing.
5112298 APHERESIS METHOD AND DEVICE Paul Prince, Willia Miller, Grant S Benjamin assigned to Baxter International Inc A simplified fluid separation method and device usable for various apheresis procedures, including plasmapheresis. At least one pump is utilized to draw a first fluid (e.g. whole blood) into a separation device. The separation device then operates to separate the fluid (e.g. whole blood) into first and second fluid fractions (e.g. a cell concentrate and blood plasma). The first and second fluid fractions are pumped from the separation device to separate first and second fluid fraction containers, both of which are positioned on a single weighing device, such as an electronic load cell. At least one of the fluid fractions is subsequently removed from its fluid fraction container and returned to the human subject or other fluid source. Weights recorded by the single weighing device are then utilized to calculate the actual weights of fluid and/or fluid fractions pumped by at least one pump during the procedure. Such actual weights of fluid and/or fluid fractions are then utilized to calculate new pump tlow constants, thereby enabling the calibration of the pump(s) to be corrected, on the basis of such new pump flow constants, prior to subsequent utilization of the pump(s) for pumping the fluid and/or fluid fractions. The single weighing device may also be utilized to monitor the weight change or rate of weight change occurring as the fluid fractions are pumped into and/or out of the fluid fraction conthereby providing a means for tainers, monitoring and verifying the pressures and flow rates within the system.
5110920
HLA TYPING METHOD AND DNA PROBES USED THEREIN Henry A Erlich assigned to Cetus Corporation HLA typing based on restriction length polymorphism is carried out by: digesting an individual’s DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled DNA hybridization probe that hybridizes to an HLA DNA sequence involved in the polymorphism; and comparing the resulting genomic blotting pattern with a standard. This technique may be adapted to make paternity or transplant or transfusion compatibility determinations or to make disease association correlations to diagnose diseases or predict susceptibility to diseases. Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci, for use in the typing procedure are described.
33924 APPARATUS AND METHOD FOR STORING AND PROCESSING BLOOD Cesare R Valeri assigned to Autologous Carp
Blood
This disclosure relates to a blood processing and preservation system. Apparatus of the system comprises a primary collection bag, a blood collection tube connected to the bag and adapted to draw a unit of blood from a person and feed the blood into the bag, and a harness connected to the bag for conducting solutions and blood components into and out of the bag. The harness includes a first tube for removing at least one blood component from the bag while retaining another component, a second tube for moving a cryoprotective solution into and out of the bag, a third tube for conducting a wash solution into and out of the bag, and identification on the bag. The foregoing apparatus is usable in a blood system including the method steps of collecting a unit of blood from a person and delivering the blood to the collection bag, processing the blood to separate the components and removing the components except for the red blood cells from the bag, preparing the red blood cells for freezing by conducting a cryoprotective solution into the
bag and mixing this solution with the red blood cells, preserving the red blood cells by freezing, subsequently washing the red blood cells by conducting a wash solution and the cells into and out of the bag, and delivering the cells in the same collection bag to a recipient. The foregoing method may be used in a homologous blood system where the recipient is not the donor, or in an autologous blood system where the blood is reinfused in the person who furnished the blood, and the identification on the bag assures the recipient that he/she is receiving his/her own blood. Other blood components, such as the plasma, may also be preserved by freezing.
5112298 APHERESIS METHOD AND DEVICE Paul Prince, Willia Miller, Grant S Benjamin assigned to Baxter International Inc A simplified fluid separation method and device usable for various apheresis procedures, including plasmapheresis. At least one pump is utilized to draw a first fluid (e.g. whole blood) into a separation device. The separation device then operates to separate the fluid (e.g. whole blood) into first and second fluid fractions (e.g. a cell concentrate and blood plasma). The first and second fluid fractions are pumped from the separation device to separate first and second fluid fraction containers, both of which are positioned on a single weighing device, such as an electronic load cell. At least one of the fluid fractions is subsequently removed from its fluid fraction container and returned to the human subject or other fluid source. Weights recorded by the single weighing device are then utilized to calculate the actual weights of fluid and/or fluid fractions pumped by at least one pump during the procedure. Such actual weights of fluid and/or fluid fractions are then utilized to calculate new pump tlow constants, thereby enabling the calibration of the pump(s) to be corrected, on the basis of such new pump flow constants, prior to subsequent utilization of the pump(s) for pumping the fluid and/or fluid fractions. The single weighing device may also be utilized to monitor the weight change or rate of weight change occurring as the fluid fractions are pumped into and/or out of the fluid fraction conthereby providing a means for tainers, monitoring and verifying the pressures and flow rates within the system.