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5204243 Recombinant poxvirus internal cores
134
PATENT ABSTRACTS
52042~ PROCESS FOR THE REDUCTION OF STAR ACTIVITIES Frank Laue, Waltraud Ankenbauer, Gudrun Sehmitz, P assigned to Boehringer Mannheim GmbH The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the D N A on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.
5204243 RECOMBINANT INTERNAL
POXVIRUS CORES
Enzo Paoletti assigned to Health Research Incorporated What is described is a modified recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, having an internal core containing D N A from a non-pox source in a nonessential region of the poxvirus genome. The recombinant poxvirus is modified by disassociating the internal core from outer membranes of the poxvirus. The D N A codes for and expresses a gene product in a cell cultured in vitro and in a host in vivo without productive replication of the virus in the cell or in the host. What is also described is a vaccine containing the modified recombinant poxvirus for inducing an immunological response in a host inoculated with the vaccine.
5204244 PRODUCTION OF CHIMERIC ANTIBODIES BY HOMOLOGOUS RECOMBINATION H Perry Fell, Bruce Kim R Folger assigned to Oncogen A process for producing chimeric antibodies using novel recombinant DNA vectors and homologous recombination in vivo is described. The recombinant DNA constructs of the inven-
tion can be used to transfect antibody producing cells so that targeted homologous recombination occurs in the transfected cells leading to gene modification and the production of chimeric antibody molecules by the transfected cells.
5204246 DNA ISOLATION
METHOD
Anil K Jhingan assigned to Pioneer Hi-Bred International Inc A novel method for the isolation of high molecular weight D N A from plants, yeast and bacteria using xanthate-forming compounds such as sodium/potassium ethyl xanthogenate is disclosed. The procedure does not require deproteination and yields clean DNA that is suitable for both PCR and Southern blotting. It can be utilized on a small scale without homogenizing the tissue. These features also facilitate automated screening of plant tissue samples, one of the labor-intensive techniques in molecular biology. This method is also adaptable for use in the field.
5204252 CANDIDA TROPICALIS TRANSFORMATION SYSTEM James M Cregg, Martin A Gleeson, Lisa Haas, Stephen Picataggio, assigned to Henkel Research Corporation A transformation system is provided for C. tropicalis, comprising constructs and microorganisms, as well as methods for preparing constructs and microorganisms, and transforming microorganisms. Particularly, a yeast transformation system comprising auxotrophic hosts which are auxotrophic in either an amino acid, purine or pyrimidine pathways and ~ employ D N A constructs comprising genes encoding biosynthetic enzymes which functionally complement the auxotrophies to prototrophies.
5204254 MALTOPENTAOSE PRODUCING AMYLASES Gerhard Schmid, Anto Candussio, August Bock, assigned to Consortium fur elektrochemische Industrie GmbH
PATENT ABSTRACTS
52042~ PROCESS FOR THE REDUCTION OF STAR ACTIVITIES Frank Laue, Waltraud Ankenbauer, Gudrun Sehmitz, P assigned to Boehringer Mannheim GmbH The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the D N A on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.
5204243 RECOMBINANT INTERNAL
POXVIRUS CORES
Enzo Paoletti assigned to Health Research Incorporated What is described is a modified recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, having an internal core containing D N A from a non-pox source in a nonessential region of the poxvirus genome. The recombinant poxvirus is modified by disassociating the internal core from outer membranes of the poxvirus. The D N A codes for and expresses a gene product in a cell cultured in vitro and in a host in vivo without productive replication of the virus in the cell or in the host. What is also described is a vaccine containing the modified recombinant poxvirus for inducing an immunological response in a host inoculated with the vaccine.
5204244 PRODUCTION OF CHIMERIC ANTIBODIES BY HOMOLOGOUS RECOMBINATION H Perry Fell, Bruce Kim R Folger assigned to Oncogen A process for producing chimeric antibodies using novel recombinant DNA vectors and homologous recombination in vivo is described. The recombinant DNA constructs of the inven-
tion can be used to transfect antibody producing cells so that targeted homologous recombination occurs in the transfected cells leading to gene modification and the production of chimeric antibody molecules by the transfected cells.
5204246 DNA ISOLATION
METHOD
Anil K Jhingan assigned to Pioneer Hi-Bred International Inc A novel method for the isolation of high molecular weight D N A from plants, yeast and bacteria using xanthate-forming compounds such as sodium/potassium ethyl xanthogenate is disclosed. The procedure does not require deproteination and yields clean DNA that is suitable for both PCR and Southern blotting. It can be utilized on a small scale without homogenizing the tissue. These features also facilitate automated screening of plant tissue samples, one of the labor-intensive techniques in molecular biology. This method is also adaptable for use in the field.
5204252 CANDIDA TROPICALIS TRANSFORMATION SYSTEM James M Cregg, Martin A Gleeson, Lisa Haas, Stephen Picataggio, assigned to Henkel Research Corporation A transformation system is provided for C. tropicalis, comprising constructs and microorganisms, as well as methods for preparing constructs and microorganisms, and transforming microorganisms. Particularly, a yeast transformation system comprising auxotrophic hosts which are auxotrophic in either an amino acid, purine or pyrimidine pathways and ~ employ D N A constructs comprising genes encoding biosynthetic enzymes which functionally complement the auxotrophies to prototrophies.
5204254 MALTOPENTAOSE PRODUCING AMYLASES Gerhard Schmid, Anto Candussio, August Bock, assigned to Consortium fur elektrochemische Industrie GmbH