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5407804 Assays for O6-methylguanine-DNA methyltransferase
PATENT ABSTRACTS modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligationtranscription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.
211
Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
5407803
5407799
METHOD FOR MEASURING THE ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME IN BIOLOGICAL SAMPLES
METHOD FOR HIGH-VOLUME SEQUENCING OF NUCLEIC ACI
Brunner Hans R; Nussberger Juer Pully, SWITZERLAND Assigned to B M R A Corporation B V
Studier F William Stony Brook, NY, UNITED STATES Assigned to Associated Universities Inc Random and directed priming methods for determining nucleotide sequences by enzymatic sequencing techniques, using libraries of primers of lengths 8, 9 or 10 bases, are disclosed. These methods permit direct sequencing of nucleic acids as large as 45,000 base pairs or larger without the necessity for subcloning. Individual primers are used repeatedly to prime sequence reactions in many different nucleic acid molecules. Libraries containing as few as 10,000 octamers, 14,200 nonamers, or 44,000 decamers would have the capacity to determine the sequence of almost any cosmid DNA. Random priming with a fixed set of primers from a smaller library can also be used to initiate the sequencing of individual nucleic acid molecules, with the sequence being completed by directed priming with primers from the library. In contrast to random cloning techniques, a combined random and directed priming strategy is far mere efficient.
54~8~ REVERSE TRANSCRIPTION WITH THERMUS THERMOPHILUS POLYMERASE Gelfand David H; Myers Thomas W Oakland, CA, UNITED STATES Assigned to Hoffmann-La Roche Inc Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, and recombinant Tth DNA polymerase.
The activity of angiotensin converting enzyme (ACE) is measured in biological samples as body fluids. ACE-activity is estimated in minimally diluted specimens, using the natural substrate angiotensin I at close to physiological concentration. Femtomoles of generated angiotensin II are trapped by specific high affinity monoclonal antibodies and thus protected from degradation by angiotensinases during the incubation step. The same antibodies are subsequently used for quantitation by radioimmunoassay.
5407804 ASSAYS FOR O6METHYLGUANINE-DNA METHYLTRANSFERASE Yarosh Daniel Merrick, NY, UNITED STATES Assigned to Applied Genetics lnc Assays for the DNA repair protein O6methylguanine-DNA methyltransferase (MGMT) are provided which employ monoclonal antibodies prepared using MGMT having transferase activity, as opposed to denatured MGMT or MGMT fragments. The monoclonal antibodies are able to recognize MGMT in single cell preparations (immunohistochemical staining assays) and in cell extracts (immunoassays). In connection with immunohistochemical staining, the use of a fluorescent readout coupled with digitization of the cell image allows for quantitative measures of MGMT levels in, for example, tumor biopsy samples. Such quantitative measures can be used to determine which patients are likely to benefit from chemotherapy using alkylating agents since tumor cells having low
PATENT ABSTRACTS
212
MGMT levels are more likely to be killed by such agents than those with high MGMT levels.
protoporphyrin IX in the cells. Also disclosed is a method for the production of protoporphyrin IX which comprises growing eukaryotic microalgae in the presence of a protoporphyrinogen oxidase inhibitor.
5407806 METHOD FOR QUANTITATIVELY MEASURING SUGAR-ALCOHOL, COLUMN AND KIT THEREOF Yabuuchi Masahiko; Akanuma Hiroshi; Masuda Minoru; Katoh Kazuo; Nakamura Tsuneo; Tajima Shigeru; Hashiba Masashi; Hayami Hiroshi; Takezawa Tomoko; Hirayama Masachik Omiya, JAPAN Assigned to Nippon Kayaku Kabushiki Kaisha A present invention relates to a method for quantitatively determining sugar-alcohols characterized by passing test samples containing sugar-alcohols, proteins, and saccharides through a column filled with basic anion-exchange resins which have a protein-removing ability and a saccharide-removing ability, and then quantitaining the sugar-alcohols in the effluent out of the column, a colunm filled with said resins and an aqueous solution of boric acid, and a kit. Sugar-alcohols such as 1,5-anhydroglucitol and the like have been calling attention as markers for diabetes mellitus recently.
5407808 METHOD AND COMPOSITION FOR PHOTODYNAMIC TREATMENT AND DETECTION OF TUMORS Hailing Blaik P; Yuhas Debra Yardley, PA, UNITED STATES Assigned to FMC Corporation Disclosed is a method and a pharmaceutical composition for killing mammalian tumor cells by subjecting the cells to light in the presence of a light-activatable tetrapyrrole, in which the improvement comprises treating the cells with a compound which inhibits the enzymatic conversion of protoporphyrinogen to protoporphyrin IX by protoporphyrinogen oxidase in the cells thereby causing a buildup of
5407809 DIGESTER FOR CONVERTING ORGANIC MATERIAL INTO COMPOST Finn Larry Gladewater, TX, UNITED STATES Assigned to Bedminster Bioconversion Corporation A rotatable digester drum for convening organic waste material into compost which has internally disposed along its longitudinal axis a series of spaced-apart baffles, each baffle having a variable cross sectional area ranging from 60 to 100% of the cross sectional area of the drum and arranged with respect to one another when in the partially open mode to cause material and air introduced into the drum to traverse a non-linear path through the drum, the drum having a plurality of longitudinally extending internal ribs arranged in spaced array around its circumference.
5407814 GENETIC FINGERPRINTING OF YEASTS Pearson Bruce M; Carter Andrew T; McKee Raymond A Norwich, UNITED KINGDOM Assigned to The Secretary of State for the Minister of Agriculture Fisheries & Food in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland The present invention relates to the identification of yeast strains by way of application of the polymerase chain reaction to amplify nucleic acid sequences characteristic of their TY transposon long terminal repeats. Polymerase chain reaction product is analysed, conveniently by agarose gel electrophoresis, and its nature related to the presence of a particular yeast strain or strain type.
211
Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
5407803
5407799
METHOD FOR MEASURING THE ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME IN BIOLOGICAL SAMPLES
METHOD FOR HIGH-VOLUME SEQUENCING OF NUCLEIC ACI
Brunner Hans R; Nussberger Juer Pully, SWITZERLAND Assigned to B M R A Corporation B V
Studier F William Stony Brook, NY, UNITED STATES Assigned to Associated Universities Inc Random and directed priming methods for determining nucleotide sequences by enzymatic sequencing techniques, using libraries of primers of lengths 8, 9 or 10 bases, are disclosed. These methods permit direct sequencing of nucleic acids as large as 45,000 base pairs or larger without the necessity for subcloning. Individual primers are used repeatedly to prime sequence reactions in many different nucleic acid molecules. Libraries containing as few as 10,000 octamers, 14,200 nonamers, or 44,000 decamers would have the capacity to determine the sequence of almost any cosmid DNA. Random priming with a fixed set of primers from a smaller library can also be used to initiate the sequencing of individual nucleic acid molecules, with the sequence being completed by directed priming with primers from the library. In contrast to random cloning techniques, a combined random and directed priming strategy is far mere efficient.
54~8~ REVERSE TRANSCRIPTION WITH THERMUS THERMOPHILUS POLYMERASE Gelfand David H; Myers Thomas W Oakland, CA, UNITED STATES Assigned to Hoffmann-La Roche Inc Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, and recombinant Tth DNA polymerase.
The activity of angiotensin converting enzyme (ACE) is measured in biological samples as body fluids. ACE-activity is estimated in minimally diluted specimens, using the natural substrate angiotensin I at close to physiological concentration. Femtomoles of generated angiotensin II are trapped by specific high affinity monoclonal antibodies and thus protected from degradation by angiotensinases during the incubation step. The same antibodies are subsequently used for quantitation by radioimmunoassay.
5407804 ASSAYS FOR O6METHYLGUANINE-DNA METHYLTRANSFERASE Yarosh Daniel Merrick, NY, UNITED STATES Assigned to Applied Genetics lnc Assays for the DNA repair protein O6methylguanine-DNA methyltransferase (MGMT) are provided which employ monoclonal antibodies prepared using MGMT having transferase activity, as opposed to denatured MGMT or MGMT fragments. The monoclonal antibodies are able to recognize MGMT in single cell preparations (immunohistochemical staining assays) and in cell extracts (immunoassays). In connection with immunohistochemical staining, the use of a fluorescent readout coupled with digitization of the cell image allows for quantitative measures of MGMT levels in, for example, tumor biopsy samples. Such quantitative measures can be used to determine which patients are likely to benefit from chemotherapy using alkylating agents since tumor cells having low
PATENT ABSTRACTS
212
MGMT levels are more likely to be killed by such agents than those with high MGMT levels.
protoporphyrin IX in the cells. Also disclosed is a method for the production of protoporphyrin IX which comprises growing eukaryotic microalgae in the presence of a protoporphyrinogen oxidase inhibitor.
5407806 METHOD FOR QUANTITATIVELY MEASURING SUGAR-ALCOHOL, COLUMN AND KIT THEREOF Yabuuchi Masahiko; Akanuma Hiroshi; Masuda Minoru; Katoh Kazuo; Nakamura Tsuneo; Tajima Shigeru; Hashiba Masashi; Hayami Hiroshi; Takezawa Tomoko; Hirayama Masachik Omiya, JAPAN Assigned to Nippon Kayaku Kabushiki Kaisha A present invention relates to a method for quantitatively determining sugar-alcohols characterized by passing test samples containing sugar-alcohols, proteins, and saccharides through a column filled with basic anion-exchange resins which have a protein-removing ability and a saccharide-removing ability, and then quantitaining the sugar-alcohols in the effluent out of the column, a colunm filled with said resins and an aqueous solution of boric acid, and a kit. Sugar-alcohols such as 1,5-anhydroglucitol and the like have been calling attention as markers for diabetes mellitus recently.
5407808 METHOD AND COMPOSITION FOR PHOTODYNAMIC TREATMENT AND DETECTION OF TUMORS Hailing Blaik P; Yuhas Debra Yardley, PA, UNITED STATES Assigned to FMC Corporation Disclosed is a method and a pharmaceutical composition for killing mammalian tumor cells by subjecting the cells to light in the presence of a light-activatable tetrapyrrole, in which the improvement comprises treating the cells with a compound which inhibits the enzymatic conversion of protoporphyrinogen to protoporphyrin IX by protoporphyrinogen oxidase in the cells thereby causing a buildup of
5407809 DIGESTER FOR CONVERTING ORGANIC MATERIAL INTO COMPOST Finn Larry Gladewater, TX, UNITED STATES Assigned to Bedminster Bioconversion Corporation A rotatable digester drum for convening organic waste material into compost which has internally disposed along its longitudinal axis a series of spaced-apart baffles, each baffle having a variable cross sectional area ranging from 60 to 100% of the cross sectional area of the drum and arranged with respect to one another when in the partially open mode to cause material and air introduced into the drum to traverse a non-linear path through the drum, the drum having a plurality of longitudinally extending internal ribs arranged in spaced array around its circumference.
5407814 GENETIC FINGERPRINTING OF YEASTS Pearson Bruce M; Carter Andrew T; McKee Raymond A Norwich, UNITED KINGDOM Assigned to The Secretary of State for the Minister of Agriculture Fisheries & Food in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland The present invention relates to the identification of yeast strains by way of application of the polymerase chain reaction to amplify nucleic acid sequences characteristic of their TY transposon long terminal repeats. Polymerase chain reaction product is analysed, conveniently by agarose gel electrophoresis, and its nature related to the presence of a particular yeast strain or strain type.