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5547835 DNA Sequencing by mass spectrometry
414
PATENT ABSTRACTS
electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to one side of the backing layer, such as carbon fibers, and a second high surface area conductive layer secured to the opposite side of the backing layer, the high surface area material layers arranged to face each other and separated by a nonconduetive, ion-permeable spacer layer to insulate electrically the backing and conductive layer. The system includes a DC power source to charge the respective conductive layers with different polarities whereby a fluid-containing material passing through the column is purified by the electrically conductive, high surface area stationary phase and the retention thereof onto the high surface area layer and permitting, for example, the purification of aqueous solutions of fiquids, such as salt, and providing for the recovery of a purified liquid.
5547702 METHOD FOR CONTINUOUS MANUFACTURE OF DIAGNOSTIC TEST STRIPS Gleisner John Lynnwood, WA, UNITED STATES Assigned to Polymer Technology International Corporation A method for making a test strip is described. The test strip is generally a substrate, a layer disposed on the substrate and located near the distal end of the substrate. The layer typically contains a chemical reagent detection system capable of detecting tile presence of a predetermined analyte in a sample of biological fluid, wherein the reagent detection system produces a detectable change in the layer in the presence of the analyte. In the method, a length of substrate is provided having a width substantially the dimension of the final length of the test strip being made; on the substrate near one edge thereof is provided a layer of material permeable to biological fluids, the layer having a width sufficient to place thereon a drop of biological fluid for treating; to the layer of permeable material is continuously appfied an aqueous solution containing reagent detection chemicals capable of providing a detectable change in the presence of a predetermined analyte,the solution being apphed in an amount to substaatially saturate the permeable material; the layer of pe~eable matmial is dried to contain the reagent detection chemicals within the layer; and
the web is cut into predetermined shorter lengths containing a preset number of test strips for further processing.
5547834 RECOMBINANT CMV NEUTRALIZING PROTEINS Spaete Richard; Pachl Carol A Belmont, CA, UNITED STATES Assigned to Chiron Corporation The present invention provides recombinant polypeptides derived from CMV glycoprotein gB and mmcated fragments thereof which contain at least one epitope which is immunoiogically identifiable with one encoded by the CMV genome. The complete characterization of the gB protein, including the identity of glycoprotein gp55, ~ permits the production of polypeptides which are useful as standards or reagents in diagnostic tests and/or as components of vaccines. This invention provides recombinant polypeptides and recombinant polynucleotides encoding these polypeptides wherein a neutralizing epitope of gB is localized within gp55.
5547835 DNA SEQUENCING BY MASS SPECTROMETRY Kuml oster Hubert Concord, MA, UNITED STATES Assigned to Sequonom Inc The invention describes a new method to sequence DNA. The improvements over the existing DNA sequencing technologies are high speed, high throughput, no electrophoresis and gel reading artifacts due to the complete absence of an electrophoretic step, and no costly reagents involving various substitutions with stable isotopes. The invention utilizes the Sanger sequencing strategy and assembles thesequence information by analysis of the nested fragments obtained by base-specific chain termination via their different molecular masses using mass spectrometry, as for example, MALDI or ES mass spectrometry. A further increase in throughput can be obtained by introducing mass-modifications in the oligonucleotide primer,
PATENT ABSTRACTS
chaln-tmninadng nueleoside lriplmsphates and/or in the chain-elongating nucleoside triphosphates, as well as using integrated tag sequences which allow multiplexing by hybrkli,~alon of tag specific probes with mass differentiated molecular weights.
5547836 ENHANCEMENT OF CHEMILUMINESCENT ASSAYS Bronstein Imna Y; Edwards Brook; Voyta John C Newton, MA, UNITED STATES Assigned to Tropix Inc Chemiluminescent bionssays for the presence or concentration of an analyte in a sample use 1,2-dioxetanes as substrates for the enzyme of an enzyme complex that bind to the analyte. The chemiluminescence obtained from the decomposition of the dioxetane triggered by the enzyme through the formation of the corresponding 1,2-dioxetane oxyanion of the enzyme complex is enhanced by the addition of TBQ as an enhancement agent. Other polymeric quaternary onium salts can be used as enhancement agents in conjunction with enhancement additives which improve the ability of the enhancement agent to form hydrophobic regions in the aqueous sample, in which regions the 1,2-dioxetane oxyanion and its chemiluminescent decomposition products can be sequestered. A kit for performing such assays is also provided.
5547838 METHOD FOR THE RAPID AND ULTRA.SENSITIVE DETECTION OF LEUKEMIC CELLS Nisson Paul; Sacchi Nicolett Gaithersburg, MD, UNFTED STATES Assigned to Life Technologies Inc An improved method is disclosed for diagnosing the presence of a chromosomal translocation characteristic of acute myelogenous leukemia. Nucleic acid molecules that may be used in this improved method are described.
415
5547839 SEQUENCING OF SURFACE IMMOBILIZED POLYMERS UTILIZING MICROFLOURESCENCE DETECTION Dowe~ William; Fedor Stephen P A Menlo Park, CA, UNITED STATES Assigned to Affymax Technologies N V Means for simultaneous parallel sequence analysis of a large number of biological polymer macxomolecules. Apparatus and methods may nse fluorescent labels in repetitive chemistry to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid substrate.
5547841 IN VITRO METHOD FOR SCREENING FOR DRUGS THAT INHIBIT PRODUCTION OR DEGRADATION OF HUMAN A4-AMYLOID Marotta Charles A; Zain Sayeeda Cambridge, MA, UNITED STAIES Assigned to The Mclean Hospital Corporation; University of Rochest The invention relates to an in vitro method of screening for drugs, potentially useful for treatment of Alzheimer's Disease. The method involves contacting a drag with a host transformed with a DNA construct which contains at least the DNA coding for human A4-amyloid peptide and overexpresses the peptide and then detecting the prevention of production or increased degradation of the A4-peptide due to the drug.
PATENT ABSTRACTS
electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to one side of the backing layer, such as carbon fibers, and a second high surface area conductive layer secured to the opposite side of the backing layer, the high surface area material layers arranged to face each other and separated by a nonconduetive, ion-permeable spacer layer to insulate electrically the backing and conductive layer. The system includes a DC power source to charge the respective conductive layers with different polarities whereby a fluid-containing material passing through the column is purified by the electrically conductive, high surface area stationary phase and the retention thereof onto the high surface area layer and permitting, for example, the purification of aqueous solutions of fiquids, such as salt, and providing for the recovery of a purified liquid.
5547702 METHOD FOR CONTINUOUS MANUFACTURE OF DIAGNOSTIC TEST STRIPS Gleisner John Lynnwood, WA, UNITED STATES Assigned to Polymer Technology International Corporation A method for making a test strip is described. The test strip is generally a substrate, a layer disposed on the substrate and located near the distal end of the substrate. The layer typically contains a chemical reagent detection system capable of detecting tile presence of a predetermined analyte in a sample of biological fluid, wherein the reagent detection system produces a detectable change in the layer in the presence of the analyte. In the method, a length of substrate is provided having a width substantially the dimension of the final length of the test strip being made; on the substrate near one edge thereof is provided a layer of material permeable to biological fluids, the layer having a width sufficient to place thereon a drop of biological fluid for treating; to the layer of permeable material is continuously appfied an aqueous solution containing reagent detection chemicals capable of providing a detectable change in the presence of a predetermined analyte,the solution being apphed in an amount to substaatially saturate the permeable material; the layer of pe~eable matmial is dried to contain the reagent detection chemicals within the layer; and
the web is cut into predetermined shorter lengths containing a preset number of test strips for further processing.
5547834 RECOMBINANT CMV NEUTRALIZING PROTEINS Spaete Richard; Pachl Carol A Belmont, CA, UNITED STATES Assigned to Chiron Corporation The present invention provides recombinant polypeptides derived from CMV glycoprotein gB and mmcated fragments thereof which contain at least one epitope which is immunoiogically identifiable with one encoded by the CMV genome. The complete characterization of the gB protein, including the identity of glycoprotein gp55, ~ permits the production of polypeptides which are useful as standards or reagents in diagnostic tests and/or as components of vaccines. This invention provides recombinant polypeptides and recombinant polynucleotides encoding these polypeptides wherein a neutralizing epitope of gB is localized within gp55.
5547835 DNA SEQUENCING BY MASS SPECTROMETRY Kuml oster Hubert Concord, MA, UNITED STATES Assigned to Sequonom Inc The invention describes a new method to sequence DNA. The improvements over the existing DNA sequencing technologies are high speed, high throughput, no electrophoresis and gel reading artifacts due to the complete absence of an electrophoretic step, and no costly reagents involving various substitutions with stable isotopes. The invention utilizes the Sanger sequencing strategy and assembles thesequence information by analysis of the nested fragments obtained by base-specific chain termination via their different molecular masses using mass spectrometry, as for example, MALDI or ES mass spectrometry. A further increase in throughput can be obtained by introducing mass-modifications in the oligonucleotide primer,
PATENT ABSTRACTS
chaln-tmninadng nueleoside lriplmsphates and/or in the chain-elongating nucleoside triphosphates, as well as using integrated tag sequences which allow multiplexing by hybrkli,~alon of tag specific probes with mass differentiated molecular weights.
5547836 ENHANCEMENT OF CHEMILUMINESCENT ASSAYS Bronstein Imna Y; Edwards Brook; Voyta John C Newton, MA, UNITED STATES Assigned to Tropix Inc Chemiluminescent bionssays for the presence or concentration of an analyte in a sample use 1,2-dioxetanes as substrates for the enzyme of an enzyme complex that bind to the analyte. The chemiluminescence obtained from the decomposition of the dioxetane triggered by the enzyme through the formation of the corresponding 1,2-dioxetane oxyanion of the enzyme complex is enhanced by the addition of TBQ as an enhancement agent. Other polymeric quaternary onium salts can be used as enhancement agents in conjunction with enhancement additives which improve the ability of the enhancement agent to form hydrophobic regions in the aqueous sample, in which regions the 1,2-dioxetane oxyanion and its chemiluminescent decomposition products can be sequestered. A kit for performing such assays is also provided.
5547838 METHOD FOR THE RAPID AND ULTRA.SENSITIVE DETECTION OF LEUKEMIC CELLS Nisson Paul; Sacchi Nicolett Gaithersburg, MD, UNFTED STATES Assigned to Life Technologies Inc An improved method is disclosed for diagnosing the presence of a chromosomal translocation characteristic of acute myelogenous leukemia. Nucleic acid molecules that may be used in this improved method are described.
415
5547839 SEQUENCING OF SURFACE IMMOBILIZED POLYMERS UTILIZING MICROFLOURESCENCE DETECTION Dowe~ William; Fedor Stephen P A Menlo Park, CA, UNITED STATES Assigned to Affymax Technologies N V Means for simultaneous parallel sequence analysis of a large number of biological polymer macxomolecules. Apparatus and methods may nse fluorescent labels in repetitive chemistry to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid substrate.
5547841 IN VITRO METHOD FOR SCREENING FOR DRUGS THAT INHIBIT PRODUCTION OR DEGRADATION OF HUMAN A4-AMYLOID Marotta Charles A; Zain Sayeeda Cambridge, MA, UNITED STAIES Assigned to The Mclean Hospital Corporation; University of Rochest The invention relates to an in vitro method of screening for drugs, potentially useful for treatment of Alzheimer's Disease. The method involves contacting a drag with a host transformed with a DNA construct which contains at least the DNA coding for human A4-amyloid peptide and overexpresses the peptide and then detecting the prevention of production or increased degradation of the A4-peptide due to the drug.