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8606406 Vectors for the expression and secretion of hirudin by transformed yeasts
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PATENT ABSTRACTS
8605817 DNA SEQUENCING Leslie Eleazer ORGEL, James Warner PATRICK assigned to THE SALK INSTITUTE FOR BIOLOGICAL STUDIES A first mixture is prepared that contains labeled chain fragments which each has a common end adjacent to a primary nucleotide and a termination at a position from the primary through an nth nucleotide, the first mixture containing nucleotide chain fragments of each length from termination at the primary through termination of the nth nucleotide. A second mixture is prepared that contains labeled chain fragments beginning at the common end and terminating at positions from the first through the nth nucleotide, the second mixture containing chain fragments of each length terminating wherever either a first or a second of the four nucleotides occurs. A third mixture is prepared that contains labeled chain fragments beginning at the common end and terminating at a position from the first through the nth nucleotide, the third mixture containing chain fragments of each length terminating wherever the first or a third of the four nucleotide sequences occurs. The chains are electropboresed with the first mixture as the center lane. This three-lane system provides a unique band pattern for each of the four nucleotides and permits the sequence to be read merely by directly comparing each of the flanking lanes with the fully stepped center lane. This system has important advantages in reducing reading errors, particularly when read with computerassisted scanning apparatus.
8606253 5-AZIDO DEOXYURIDINE COMPOUNDS AND METHOD OF SYNTHESIS OF THE SAME Boyd E HALEY, Robert K EVANS assigned to THE UNIVERSITY OF WYOMING 5-azido-2"-deoxyuridine nucleoside and nucleotide compounds and a method of producing them. The method involves reacting a 5"deoxyuridine compound with nitrosonium tetrafluoroborate to produce a 5-nitrodeoxyuridine compound. The 5-nitrodeoxyuridine compound is reduced to a 5amino-deoxyuridine compound in the presence of metallic zinc. The 5-amino-deoxyuridine compound is acidified and reacted with sodium nitrite to produce a 5-diazo-deoxyuridine compound. The diazo-deoxyuridine compound is then reacted with sodium azide to produce a 5azido-deoxyuridine compound. The latter, in the
monophosphate form, is further reacted with diphenylchlorophosphate to produce the photoactive triphosphate, 5-azido-2"-deoxyuridine-5"triphosphate or 5-azido-dUTP. The photoactive compound, when added to DNA in an in vitro synthesis reaction, produces photoactive DNA.
8606405 NOVEL TRANSFORMANT ITS USE
AND
Reiko SASADA, Haruo ONDA, Koichi IGARASHI, Kodan-jutaku 3-811, 48-3, Mibubojo-cho, Nakagyo-ku. Kyoto-shi, Kyoto 604, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD; Animal cells transformed by DNA having a promoter in a region which codes human IL-2 protein and in an upstream region thereoL and a process for producing human IL-2 protein by culturing the transformant to produce and accumulate human IL-2 protein in the culture product, and collecting the product. The protein is useful as an agent for preventing and treating tumor and an agent for treating a disease of immunity function reduction.
8600406 VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY T R A N S F O R M E D Y E A S T S G@rard LOISON, Paul TOLSTOSHEV. Yves LEMOINE, Jean-Pierre LECOCQ. 1, rue de I'Aimant, F-67000 Strasbourg, France assigned to TRANSGENE S A; Functional block of DNA enabling to prepare hirudine from yeast, characterized in that it comprises at least: - the gene of hirudine or one of its variants (gene H); - A DNA sequence (S)tr() comprising the signals providing for the transcription of the gene H by the yeast.
8606407 I D E N T I F I C A T I O N O F A N IGEBINDING P R O T E I N BY MOLECULAR CLONING Fu-Tong LU assigned to MEDICAL BIOLOGY INSTITUTE A polypeptide which selectively binds lgE having a molecular weight of about 31,000 daltons, characterized in that it reacts with anti-
PATENT ABSTRACTS
8605817 DNA SEQUENCING Leslie Eleazer ORGEL, James Warner PATRICK assigned to THE SALK INSTITUTE FOR BIOLOGICAL STUDIES A first mixture is prepared that contains labeled chain fragments which each has a common end adjacent to a primary nucleotide and a termination at a position from the primary through an nth nucleotide, the first mixture containing nucleotide chain fragments of each length from termination at the primary through termination of the nth nucleotide. A second mixture is prepared that contains labeled chain fragments beginning at the common end and terminating at positions from the first through the nth nucleotide, the second mixture containing chain fragments of each length terminating wherever either a first or a second of the four nucleotides occurs. A third mixture is prepared that contains labeled chain fragments beginning at the common end and terminating at a position from the first through the nth nucleotide, the third mixture containing chain fragments of each length terminating wherever the first or a third of the four nucleotide sequences occurs. The chains are electropboresed with the first mixture as the center lane. This three-lane system provides a unique band pattern for each of the four nucleotides and permits the sequence to be read merely by directly comparing each of the flanking lanes with the fully stepped center lane. This system has important advantages in reducing reading errors, particularly when read with computerassisted scanning apparatus.
8606253 5-AZIDO DEOXYURIDINE COMPOUNDS AND METHOD OF SYNTHESIS OF THE SAME Boyd E HALEY, Robert K EVANS assigned to THE UNIVERSITY OF WYOMING 5-azido-2"-deoxyuridine nucleoside and nucleotide compounds and a method of producing them. The method involves reacting a 5"deoxyuridine compound with nitrosonium tetrafluoroborate to produce a 5-nitrodeoxyuridine compound. The 5-nitrodeoxyuridine compound is reduced to a 5amino-deoxyuridine compound in the presence of metallic zinc. The 5-amino-deoxyuridine compound is acidified and reacted with sodium nitrite to produce a 5-diazo-deoxyuridine compound. The diazo-deoxyuridine compound is then reacted with sodium azide to produce a 5azido-deoxyuridine compound. The latter, in the
monophosphate form, is further reacted with diphenylchlorophosphate to produce the photoactive triphosphate, 5-azido-2"-deoxyuridine-5"triphosphate or 5-azido-dUTP. The photoactive compound, when added to DNA in an in vitro synthesis reaction, produces photoactive DNA.
8606405 NOVEL TRANSFORMANT ITS USE
AND
Reiko SASADA, Haruo ONDA, Koichi IGARASHI, Kodan-jutaku 3-811, 48-3, Mibubojo-cho, Nakagyo-ku. Kyoto-shi, Kyoto 604, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD; Animal cells transformed by DNA having a promoter in a region which codes human IL-2 protein and in an upstream region thereoL and a process for producing human IL-2 protein by culturing the transformant to produce and accumulate human IL-2 protein in the culture product, and collecting the product. The protein is useful as an agent for preventing and treating tumor and an agent for treating a disease of immunity function reduction.
8600406 VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY T R A N S F O R M E D Y E A S T S G@rard LOISON, Paul TOLSTOSHEV. Yves LEMOINE, Jean-Pierre LECOCQ. 1, rue de I'Aimant, F-67000 Strasbourg, France assigned to TRANSGENE S A; Functional block of DNA enabling to prepare hirudine from yeast, characterized in that it comprises at least: - the gene of hirudine or one of its variants (gene H); - A DNA sequence (S)tr() comprising the signals providing for the transcription of the gene H by the yeast.
8606407 I D E N T I F I C A T I O N O F A N IGEBINDING P R O T E I N BY MOLECULAR CLONING Fu-Tong LU assigned to MEDICAL BIOLOGY INSTITUTE A polypeptide which selectively binds lgE having a molecular weight of about 31,000 daltons, characterized in that it reacts with anti-