5432082 Expression and secretion vector in yeasts, useful for preparing heterologous proteins

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PATENT ABSTRACTS

5432082 EXPRESSION AND SECRETION VECTOR IN YEASTS, USEFUL FOR PREPARING HETEROLOGOUS PROTEINS

acetylcboline receptor, and ion channels. The peptides are of such length that they can be made by chemical synthesis. They also may be made using recombinant DNA techniques, and the DNA encoding such conotoxins having pesticidal properties can be incorporated as plant defense genes into plant species of interest.

Galeotti Cesira; Paila Emanuela; Raugei Giovanni; Bensi Giuliano; Melli Mafia L Siena, ITALY Assigned to Sclavo S p A The invention provides an expression and secretion vector in yeasts which is useful for preparing heterologous proteins, comprising a synthetic oligonucleotide which directs the secretion of the heterologous protein wherein the synthetic oligonucleotide is positioned between the inducible hybrid promoter GAL-CYC and a multiple-site polylinker followed by the signals of transcription termination recognized by the RNA polymerase of the yeasts. The invention includes a hybrid plasmid obtained by cloning in one of the restriction sites of the polylinker of the vector, the DNA sequence which codes for a heterologous protein. A process is also described for the preparation of heterologous proteins which comprises cultivating in a suitable culture medium a yeast transformed with the hybrid plasmid and recovering from the culture medium the resulting heterologous proteins. The recovered proteins include hormones, lymphokines, viral antigens or immunogens useful in the therapeutical and diagnostic field. 5432155 CONOTOXINS

5432157 CHRYSOSPERMINS, ACTIVE PEPTIDES FROM APIOCI/_EA CHRYSOSPERMA HAVING A PHARMACOLOGICAL EFFECT AND A USE THEREOF

Metzger Jorg; Schlegel Brigitt; Fleck Wemer F; Domberger Klansjurge; Ihn Wolfgang; Schade Wolfgang; Grafe Udo J GERMANAY Assigned to Hoechst Aktiengesellschaft The invention relates to the chrysospermin active peptides of the formula I (*See Patent for Tabular Presentation*) PS in which x and y, independently of each other, denote Aib or Iva, which are synthesized by the fungus Apiocrea chrysosperma during fermentation and which accumulate in the culture broth, to a preparation process, to the use of the chrysospermins as pharmacological active compounds, in particular as active compounds against bacteria, fungi and/or nematodes, and for the treatment of rejection reactions in organ transplantations and of tumor disorders, and to the microorganism Apiocrea chrysosperma DSM 7444.

I

Olivera Baldomero; Rivier Jean E F; Cruz Lourdes J; Abogadie F; Hopkins Chris E; Dykert John; Tortes Josep L Salt Lake City, UT, UNITED STATES Assigned to The Salk Institute For Biological Studies; University of Utah Research Foundation Substantially pure conotoxins are provided which inhibit synaptic transmissions at the neuromuscular junctions and which are useful both in vivo and in assays because they specifically target particular receptors, such as the

5432193 ANTIBIOTIC

LL-D37187 ALPHA

Carter Guy T; Schlingmann Gerhar; Labeda David P; Goodman Joseph J Suffern, NY, UNITED STATES Assigned to American Cyanamid Company This invention relates to antibiotic LL-D37187 alpha derived from the microorganism Streptomyces which is useful as an antibacterial agent.