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8607380 Process for preparing polypeptide
192
PATENT ABSTRACTS
8606959 LIPOSOME INHALATION METHOD AND SYSTEM Paul J MIHALKO, Robert M ABRA assigned to LIPOSOME TECHNOLOGY INC: A method and system for administering a drug by inhalation. The system includes a suspension of liposomes containing the drug predominantly in a liposome-entrapped form. where the liposomes have a phospholipid composition selected to produce a desired drug-release rate when administered to the respiratory tract. The invention is useful in delivering a drug systematically, under controlled drug-release rates, as well as to a target site in the respiratory tract.
8607089 MURINE HYBRIDOMA LYM-2 AND DIAGNOSTIC ANTIBODY PRODUCED THEREBY Alan L EPSTEIN. Alan L EPSTEIN assigned to NORTHWESTERN UNIVERSITY Hybridoma Lym-2 (ATCC No. HB 8613) produces murine lgGI monoclonal antobodies specifically against normal human B cells and derived malignancies. The Lym-2 antibodies have possible clinical utility for the in vivo diagnosis and therapy of human B-cell lymphomas and leukemias.
8607154 8607062 MONOCLONAL ANTIBODY TO DECAY ACCELERATING FACTOR (DAF), A M E T H O D F O R M A K I N G IT, AND USE Taroh KINOSHITA, M Edward MEDOF. Victor NUSSENZWEIG assigned to NEW YORK UNIVERSITY A monoclonal antibody immunochemically reactive with decay accelerating factor and a method for making such a monoclonal antibody by purifying decay accelerating factor to homogeneity by a combination of anion-exchange and hydrophobic interaction chromatography, gel filtration, lectin-Sepharose chromatography. immunoaffinity chromatography, anion exchange chromatography and high-performance liquid chromatography; immunizing mice with said purified factor: fusing spleen cells from said mice with mouse myeloma cells to produce hybrid cells: and screening said hybrid cells to identify those secreting said monoclonal antibody.
8607088 MURINE HYBRIDOMA LYM-I AND DIAGNOSTIC ANTIBODY PRODUCED THEREBY Alan L EPSTEIN. Alan L EPSTEIN assigned to NORTHWESTERN UNIVERSITY Hybridoma Lym-I (ATCC No. HB 8612) produces murine IgG2a monoclonal antibodies specifically against normal human B cells and derived malignancies. The Lym-I antibodies have clinical utility for the in vivo diagnosis and therapy of human B-cell lymphomas and leukemias.
METHOD FOR ASSAYING HUMAN PULMONARY SURFACE ACTIVE SUBSTANCE AND REAGENT KIT THEREFOR Toyoaki AKINO, Yoshio KUROKI, Kenji HOSODA, Hideaki SUZUKI. 4-1, Nishino I-jo 4-chome. Nishi-ku. Sapporo-shi. Hokkaido 063, Japan assigned to TEIJIN LIMITED A method for immunologically assaying a human pulmonary surface active substance. which comprises using a first monoclonal antibody which recognizes apoprotein of the human pulmonary surface active substance and a second labeled monoclonal antibody which recognizes said apoprotein but binds to an antigen site different from that to which the first monoclonal antibody binds, and a kit for said method.
8607380 PROCESS FOR PREPARING POLYPEPTIDE Shizue N A K A G A W A , Takao YAMADA, Osamu NISHIMURA, 6-23-709. Nankonaka 5chome, Suminoe-ku, Osaka-shi. Osaka 559, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD: A process for preparing a polypeptide represented by H-X-Pro-Y-OH (wherein X represents an amino acid other than Pro. and Y represents a peptide chain), which comprises reacting aminopeptidase with a polypeptide represented by HMet-X-Pro-Y-OH (wherein X and Y are as defined above). This process enables to specifically remove methionine at the amino terminal of the polypeptide. Therefore. poly-
193
PATENT ABSTRACTS peptides of a natural amino acid sequence can be produced with industrial advantage from polypeptides produced, for example, by genetic engineering technology.
8607381 MONOCLONAL ANTIBODY TO MHS-5: A N E W P R O B E F O R SEXUAL ASSAULT ANALYSES John C HERR, Mark SIGMAN, William M SUTHERLAND assigned to THE UNIVERSITY OF VIRGINIA ALUMNI PATENTS FOUNDATI A new probe for forensic analysis of sexual assaults. The probe is a monoclonal antibody to a new protein marker, MHS-5, in semen. Also disclosed is the bybridoma producing the antibody as well as an assay utilizing the antibody for forensic analysis of criminal evidence.
conventional dosage forms for peptide vaccines. or can be supplied in a recombinant vaccinia and the peptides synthesized in situ. Particularly useful autoantigens are those derived from hormones controlling reproduction. An easily administered and reversible vaccine for preventing human pregnancy is disclosed. The vaccine comprises a vaccinia virus vector modified to contain the DNA sequence encoding the Cterminal portion (CTP) of the beta-chain of human chorionic gonadotropin, or a multimer thereof. The vaccinia vectors are designed so that the encoded antigen is expressed internally by infected cells, or the CTP-encoding sequence is modified to provide a chimera which is capable of carrying the CTP)n( antigen to the surface of the infected cell and either presenting the CTP)n(antigen at the cell surface or of secreting it. In a preferred embodiment, the influenza hemagglutinin protein, or portions thereof, are used as the components of the chimera. Similar constructions which include alternate hormone derived sequences are also effective in humans and in other mammals. The antibodies obtained against and specific for these autoantigens are also useful.
8607~2 PROTECTIVE HUMAN MONOCLONAL ANTIBODIES TO PSEUDOMONAS AERUGINOSA) EXOTOXIN A Anthony W SIADAK, Mark E LOSTROM assigned to GENETIC SYSTEMS CORPORATION Cell lines have been produced that secrete human monoclonal antibodies capable of binding to and neutralizing Pseudomanas aeruginosa) exotoxin A. These antibodies have been found to be protective against lethal challenges of the exotoxin. Pharmaceutical compositions containing these antibodies, which can be in combination with other monoclonal antibodies, blood plasma fractions and antimicrobial agents, and the prophylactic and therapeutic use of such compositions in the management of infections are included.
8~7~4 PROCESS FOR PREPARING HAPATITIS B VIRUS SURFACE A N T I G E N P31 Masakazu KIKUCHI. Yukio FUJISAWA, Shuichi IKEYAMA. Osamu NISHIMURA, 416, Higashitokiwadai 7-chome, Toyono-cho, Toyono-gun. Osaka 563-01. Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD; Hepatitis B virus surface antigen P31 is prepared by culturing transformant containing recombinant DNA prepared by inserting P31-coding DNA to T-terminal of a promoter region.
8607464 METHODS FOR IDENTIFYING ALLELES ASSOCIATED WITH INCREASED RISK OF DIABETES
8~73~ AUTOANTIGEN
VACCINES
Karen D TALMADGE, John C FIDDES assigned to BIOTECHNOLOGY RESEARCH PARTNERS LTD; Autoantigen vaccines are obtained by conferring antigenicity by formation of multimers, of fusion proteins to non-bacterial sequences, or both. The vaccines can be administered using
Gerald T NEPOM, Barbara S NEPOM, Karen A NELSON assigned to GENETIC SYSTEMS CORPORATION Methods for identifying individuals at increased risk of diabetes. The methods utilize the discovery of the DQw3.2 variant, which identifies a specific allelic polymorphism at a single gene locus. One preferred method utilizes a labeled probe to detect the DQw3.2 allele. This method involves estimating the size of the hybridizable
PATENT ABSTRACTS
8606959 LIPOSOME INHALATION METHOD AND SYSTEM Paul J MIHALKO, Robert M ABRA assigned to LIPOSOME TECHNOLOGY INC: A method and system for administering a drug by inhalation. The system includes a suspension of liposomes containing the drug predominantly in a liposome-entrapped form. where the liposomes have a phospholipid composition selected to produce a desired drug-release rate when administered to the respiratory tract. The invention is useful in delivering a drug systematically, under controlled drug-release rates, as well as to a target site in the respiratory tract.
8607089 MURINE HYBRIDOMA LYM-2 AND DIAGNOSTIC ANTIBODY PRODUCED THEREBY Alan L EPSTEIN. Alan L EPSTEIN assigned to NORTHWESTERN UNIVERSITY Hybridoma Lym-2 (ATCC No. HB 8613) produces murine lgGI monoclonal antobodies specifically against normal human B cells and derived malignancies. The Lym-2 antibodies have possible clinical utility for the in vivo diagnosis and therapy of human B-cell lymphomas and leukemias.
8607154 8607062 MONOCLONAL ANTIBODY TO DECAY ACCELERATING FACTOR (DAF), A M E T H O D F O R M A K I N G IT, AND USE Taroh KINOSHITA, M Edward MEDOF. Victor NUSSENZWEIG assigned to NEW YORK UNIVERSITY A monoclonal antibody immunochemically reactive with decay accelerating factor and a method for making such a monoclonal antibody by purifying decay accelerating factor to homogeneity by a combination of anion-exchange and hydrophobic interaction chromatography, gel filtration, lectin-Sepharose chromatography. immunoaffinity chromatography, anion exchange chromatography and high-performance liquid chromatography; immunizing mice with said purified factor: fusing spleen cells from said mice with mouse myeloma cells to produce hybrid cells: and screening said hybrid cells to identify those secreting said monoclonal antibody.
8607088 MURINE HYBRIDOMA LYM-I AND DIAGNOSTIC ANTIBODY PRODUCED THEREBY Alan L EPSTEIN. Alan L EPSTEIN assigned to NORTHWESTERN UNIVERSITY Hybridoma Lym-I (ATCC No. HB 8612) produces murine IgG2a monoclonal antibodies specifically against normal human B cells and derived malignancies. The Lym-I antibodies have clinical utility for the in vivo diagnosis and therapy of human B-cell lymphomas and leukemias.
METHOD FOR ASSAYING HUMAN PULMONARY SURFACE ACTIVE SUBSTANCE AND REAGENT KIT THEREFOR Toyoaki AKINO, Yoshio KUROKI, Kenji HOSODA, Hideaki SUZUKI. 4-1, Nishino I-jo 4-chome. Nishi-ku. Sapporo-shi. Hokkaido 063, Japan assigned to TEIJIN LIMITED A method for immunologically assaying a human pulmonary surface active substance. which comprises using a first monoclonal antibody which recognizes apoprotein of the human pulmonary surface active substance and a second labeled monoclonal antibody which recognizes said apoprotein but binds to an antigen site different from that to which the first monoclonal antibody binds, and a kit for said method.
8607380 PROCESS FOR PREPARING POLYPEPTIDE Shizue N A K A G A W A , Takao YAMADA, Osamu NISHIMURA, 6-23-709. Nankonaka 5chome, Suminoe-ku, Osaka-shi. Osaka 559, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD: A process for preparing a polypeptide represented by H-X-Pro-Y-OH (wherein X represents an amino acid other than Pro. and Y represents a peptide chain), which comprises reacting aminopeptidase with a polypeptide represented by HMet-X-Pro-Y-OH (wherein X and Y are as defined above). This process enables to specifically remove methionine at the amino terminal of the polypeptide. Therefore. poly-
193
PATENT ABSTRACTS peptides of a natural amino acid sequence can be produced with industrial advantage from polypeptides produced, for example, by genetic engineering technology.
8607381 MONOCLONAL ANTIBODY TO MHS-5: A N E W P R O B E F O R SEXUAL ASSAULT ANALYSES John C HERR, Mark SIGMAN, William M SUTHERLAND assigned to THE UNIVERSITY OF VIRGINIA ALUMNI PATENTS FOUNDATI A new probe for forensic analysis of sexual assaults. The probe is a monoclonal antibody to a new protein marker, MHS-5, in semen. Also disclosed is the bybridoma producing the antibody as well as an assay utilizing the antibody for forensic analysis of criminal evidence.
conventional dosage forms for peptide vaccines. or can be supplied in a recombinant vaccinia and the peptides synthesized in situ. Particularly useful autoantigens are those derived from hormones controlling reproduction. An easily administered and reversible vaccine for preventing human pregnancy is disclosed. The vaccine comprises a vaccinia virus vector modified to contain the DNA sequence encoding the Cterminal portion (CTP) of the beta-chain of human chorionic gonadotropin, or a multimer thereof. The vaccinia vectors are designed so that the encoded antigen is expressed internally by infected cells, or the CTP-encoding sequence is modified to provide a chimera which is capable of carrying the CTP)n( antigen to the surface of the infected cell and either presenting the CTP)n(antigen at the cell surface or of secreting it. In a preferred embodiment, the influenza hemagglutinin protein, or portions thereof, are used as the components of the chimera. Similar constructions which include alternate hormone derived sequences are also effective in humans and in other mammals. The antibodies obtained against and specific for these autoantigens are also useful.
8607~2 PROTECTIVE HUMAN MONOCLONAL ANTIBODIES TO PSEUDOMONAS AERUGINOSA) EXOTOXIN A Anthony W SIADAK, Mark E LOSTROM assigned to GENETIC SYSTEMS CORPORATION Cell lines have been produced that secrete human monoclonal antibodies capable of binding to and neutralizing Pseudomanas aeruginosa) exotoxin A. These antibodies have been found to be protective against lethal challenges of the exotoxin. Pharmaceutical compositions containing these antibodies, which can be in combination with other monoclonal antibodies, blood plasma fractions and antimicrobial agents, and the prophylactic and therapeutic use of such compositions in the management of infections are included.
8~7~4 PROCESS FOR PREPARING HAPATITIS B VIRUS SURFACE A N T I G E N P31 Masakazu KIKUCHI. Yukio FUJISAWA, Shuichi IKEYAMA. Osamu NISHIMURA, 416, Higashitokiwadai 7-chome, Toyono-cho, Toyono-gun. Osaka 563-01. Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD; Hepatitis B virus surface antigen P31 is prepared by culturing transformant containing recombinant DNA prepared by inserting P31-coding DNA to T-terminal of a promoter region.
8607464 METHODS FOR IDENTIFYING ALLELES ASSOCIATED WITH INCREASED RISK OF DIABETES
8~73~ AUTOANTIGEN
VACCINES
Karen D TALMADGE, John C FIDDES assigned to BIOTECHNOLOGY RESEARCH PARTNERS LTD; Autoantigen vaccines are obtained by conferring antigenicity by formation of multimers, of fusion proteins to non-bacterial sequences, or both. The vaccines can be administered using
Gerald T NEPOM, Barbara S NEPOM, Karen A NELSON assigned to GENETIC SYSTEMS CORPORATION Methods for identifying individuals at increased risk of diabetes. The methods utilize the discovery of the DQw3.2 variant, which identifies a specific allelic polymorphism at a single gene locus. One preferred method utilizes a labeled probe to detect the DQw3.2 allele. This method involves estimating the size of the hybridizable