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Analysis of SCAR marker nucleotide sequences in maize (Zea mays L.) somaclones
SUPPLEMENTARY MATERIAL Supplementary table 1. Morphological and physiological traits changed in somaclones. Somaclones Characters
Plant height
The first group * (two months of culturing) R11 R14 R27 R54 + +
The second group ** (eight months of culturing) R105 R106 R107 R119 +
Number of leaves
+
+
+
+
Length of leaf
+
+
+
+
+
+
+
+
+
+
Width of leaf Height of tassel
+
+
+
Number of tassel branches
+
+
Number of kernel rows in spadix Number of kernel in the row
+
+ +
+
+
+
+
+
+
+
Length of spadix Weight of 100 kernels Capability to form embryogenic callus Flowering time Kernel coloration
+ +
+
Changes of particular characters are indicated by +. All changes were stable inherited. * Yu.I. Dolgikh, S.N. Larina, Yu.V. Bolonkina, Analysis of somaclonal variability of inbred maize line A188, Russ. J. Genet. 28 (1992) 74-82. ** Yu.I. Dolgikh, Somaclonal variability of plants and possibility of its practical application (on the maize model), Dissertation, K.A.Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (2005).
29
Supplementary table 2. Inheritance of SCAR markers in somaclones and presence of them in original A188 maize line. The original line, regenerants, hybrids
No. of plants with marker fragment : without a fragment
Fragment QR
Generations Q-20
A188
M-10
OPC-09
NO-15
Leb
-*
2
A
2+A
R11
R1 R2
0:8 0:4 0:20
0:8 4:0 20:0
8:0 0:4 0:20
0:8 0:4 0:20
8:0 2:2 16:4
0 0 0
0 4 20
8 0 0
0 0 0
R14
R1 R2
0:8 0:12
8:0 12:0
0:8 0:12
0:8 0:12
6:2 0:12
0 0
8 12
0 0
0 0
R1 R2 R3 R4
0:20 0:21 0:14 0:21
20:0 21:0 14:0 21:0
0:20 0:21 0:14 0:21
0:20 0:21 0:14 0:21
17:3 8:13 5:9 0:21
0 0 0 0
20 21 14 21
0 0 0 0
0 0 0 0
F27X188
F1 F2
0:19 0:35
19:0 29:6
19:0 27:8
0:19 0:35
9:10 14:21
0 0
2 7
0 8
17 20
R54
R1 R2
0:4 0:20
4:0 20:0
0:4 0:20
0:4 0:20
3:1 0:20
0 0
4 20
0 0
0 0
R105
R1 R2
7:0 20:0
0:7 0:20
0:7 0:20
3:4 16:4
2:5 15:5
0 0
7 20
0 0
0 0
F105x188
F1 F2
4:0 35:0
0:4 0:35
4:0 29:6
4:0 27:8
4:0 29:6
0 0
1 8
0 8
3 19
R107
R1 R2 R3 R4 R1 R2
8:0 19:0 20:0 20:0 15:0 20:0
0:8 0:19 0:20 0:20 0:15 0:20
3:5 14:5 9:11 16:4 0:15 0:20
0:8 0:19 0:20 0:20 12:3 15:5
4:4 18:1 15:5 18:2 12:3 14:6
0 0 0 0 0 0
5 3 10 6 15 20
0 1 1 5 0 0
3 15 9 9 0 0
R119
R1 R2
18:0 21:0
0:18 0:21
2:16 0:21
0:18 0:21
16:2 7:14
0 0
16 21
0 0
2 0
R27
R106
* - absence of any fragment;
30
Plant height
The first group * (two months of culturing) R11 R14 R27 R54 + +
The second group ** (eight months of culturing) R105 R106 R107 R119 +
Number of leaves
+
+
+
+
Length of leaf
+
+
+
+
+
+
+
+
+
+
Width of leaf Height of tassel
+
+
+
Number of tassel branches
+
+
Number of kernel rows in spadix Number of kernel in the row
+
+ +
+
+
+
+
+
+
+
Length of spadix Weight of 100 kernels Capability to form embryogenic callus Flowering time Kernel coloration
+ +
+
Changes of particular characters are indicated by +. All changes were stable inherited. * Yu.I. Dolgikh, S.N. Larina, Yu.V. Bolonkina, Analysis of somaclonal variability of inbred maize line A188, Russ. J. Genet. 28 (1992) 74-82. ** Yu.I. Dolgikh, Somaclonal variability of plants and possibility of its practical application (on the maize model), Dissertation, K.A.Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (2005).
29
Supplementary table 2. Inheritance of SCAR markers in somaclones and presence of them in original A188 maize line. The original line, regenerants, hybrids
No. of plants with marker fragment : without a fragment
Fragment QR
Generations Q-20
A188
M-10
OPC-09
NO-15
Leb
-*
2
A
2+A
R11
R1 R2
0:8 0:4 0:20
0:8 4:0 20:0
8:0 0:4 0:20
0:8 0:4 0:20
8:0 2:2 16:4
0 0 0
0 4 20
8 0 0
0 0 0
R14
R1 R2
0:8 0:12
8:0 12:0
0:8 0:12
0:8 0:12
6:2 0:12
0 0
8 12
0 0
0 0
R1 R2 R3 R4
0:20 0:21 0:14 0:21
20:0 21:0 14:0 21:0
0:20 0:21 0:14 0:21
0:20 0:21 0:14 0:21
17:3 8:13 5:9 0:21
0 0 0 0
20 21 14 21
0 0 0 0
0 0 0 0
F27X188
F1 F2
0:19 0:35
19:0 29:6
19:0 27:8
0:19 0:35
9:10 14:21
0 0
2 7
0 8
17 20
R54
R1 R2
0:4 0:20
4:0 20:0
0:4 0:20
0:4 0:20
3:1 0:20
0 0
4 20
0 0
0 0
R105
R1 R2
7:0 20:0
0:7 0:20
0:7 0:20
3:4 16:4
2:5 15:5
0 0
7 20
0 0
0 0
F105x188
F1 F2
4:0 35:0
0:4 0:35
4:0 29:6
4:0 27:8
4:0 29:6
0 0
1 8
0 8
3 19
R107
R1 R2 R3 R4 R1 R2
8:0 19:0 20:0 20:0 15:0 20:0
0:8 0:19 0:20 0:20 0:15 0:20
3:5 14:5 9:11 16:4 0:15 0:20
0:8 0:19 0:20 0:20 12:3 15:5
4:4 18:1 15:5 18:2 12:3 14:6
0 0 0 0 0 0
5 3 10 6 15 20
0 1 1 5 0 0
3 15 9 9 0 0
R119
R1 R2
18:0 21:0
0:18 0:21
2:16 0:21
0:18 0:21
16:2 7:14
0 0
16 21
0 0
2 0
R27
R106
* - absence of any fragment;
30