- Email: [email protected]
Breast cancer and dietary patterns
Abstracts / Journal of Cellular Immunotherapy 3 (2017) 2e30
our hospital with anorexia, diplopia, lethargy, and right leg claudication. Hodgkin's lymphoma was in remission phase for about 7 years. Six months before admission, he was admitted to hospital because of fever, night sweats, fatigue, weight loss, splenomegaly, and multiple cervical, retroperitoneal and para-aortic lymphadenopathy that brought up a presumptive diagnosis of relapsed phase of Hodgkin's disease and he received chemotherapy for 4 months. On the tenth day of hospitalization, the temperature briefly rose to 39 oC and photophobia, nuchal, passive and active foot rigidity developed. On a neurological examination, outward deviation, mydriasis, ptosis and lack of response to light were observed in the right eye. Other cranial nerves were intact. His motor strength was 5/5 in the left extremities and 4/5 in the right extremities with normal sensation. He showed dysmetria and mild hyperreflexia on the right side. Tremor on left hand with mild rigidity on left limbs was observed. Psychiatric examination also revealed depression and anxiety disorders. All necessary tests such as CBC and Serological tests, CT scan and MRI were performed. According to the basal ganglia and cerebral peduncle involvement, we suspected brain metastases arising from Hodgkin's lymphoma so the patient was candidate for brain biopsy. When the patient was treated, a prompt therapeutic response was noted. After anti-Toxoplasma therapy initiation, progression of disease was stopped, his speech was dramatically improved but motor sequelaes remained. MRI of the brain at 6 weeks after anti-toxoplasmosis therapy showed dramatically regression of the lesions, typical for toxoplasmosis. Diffuse toxoplasmosis with CNS involvement is a life-threatening disease which often avoids diagnosis so; early diagnosis of the disease has been rewarded by a rapid response to treatment. According to Centers for Disease Control and Prevention (CDC) guidelines, criteria for establishing the diagnosis of cerebral toxoplasmosis includes proper clinical features, consistent findings on imaging, positive serological studies, and a therapeutic response to treatment. All these four criteria existed in our patient. The most frequent therapy for cerebral toxoplasmosis is pyrimethamine and sulfadiazine along with folinic acid to reduce hematological complications of pyrimethamine. Our patient showed good clinical improvement within 4 days and the lesion resolved approximately within 3 weeks. A positive response to treatment can be demonstrated both clinically and radiologically.
Keywords: Nervous system, Toxoplasmosis, Hodgkin's lymphoma BREAST CANCER AND DIETARY PATTERNS Elham Dindarlou Undergraduate student. Department of Biology, Young Researchers and Elite club, Zarghan Branch, Islamic Azad University, Zarghan, Iran E-mail address: [email protected]
Extended Abstract Breast cancer is the most frequently cancer and the second cause of mortality in women. The etiology of breast cancer is multifactorial, and genetic, environmental and lifestyle factors promote the growth of cancer cells. It can occur in both men and women, but it is much more common in women. Breast cancer comprises 22.9% of invasive cancers in women and 16% of all female cancers. It is suspected that diet influences the risk of getting breast cancer. Evidence is inconclusive concerning the role of food groups, as distinct from that of major nutrients, in the etiology of breast cancer in women. Lifestyle changes have been shown to decrease breast cancer risk even in high-risk women. Measures to decrease the risk include limiting alcohol consumption, controlling body weight, being physically active, and breastfeeding. Eating a healthy diet might reduce our risk of breast cancer. A healthy diet includes plenty of vegetable, fruits, soy, green tea, antioxidant, and less fat. Women who have inherited changes in the BRCA1 and BRCA2 genes have a higher risk of breast cancer and they should get an MRI and a mammogram every year. For them exposure to radiation, such as chest Xrays, may increase the risk of breast cancer. Ovarian hormones are biological markers for breast cancer risk. Soybean consumption can be responsible in part for lower levels of ovarian
7
hormones and decreased risk of breast cancer in women. No food or diet can prevent you from getting breast cancer. But some foods can make your body the healthiest it can be, and help keep your risk for breast cancer as low as possible. Some of them are: Replace animal fats with polyunsaturated fats (in many vegetable oil) Eat more isoflavones Make sure you have enough calcium in your diet Eat plenty of fruit and vegetables Antioxidant are nutrients that can slow oxidative damage to our body's tissues. Antioxidants can help prevent breast cancer and they are found in plant foods as bright, orange carrots and purple blueberries. The most common antioxidants are vitamin A, C and E, beta carotene, selenium and lycopene. Lycopene found in red fruit and vegetables like grapefruit, watermelon and Beta-carotene found in vegetables that are orange like carrots, mango, and peach. Green tea and soy isoflavones can reduce the risk of breast and other cancers. Epidemiologic evidence suggests that vitamin D may play a role in decreasing breast cancer risk. As Antioxidants have anti-cancer properties, it recommended in daily diet. Selenium is a mineral salt that found in mushroom, spinach, beef liver, nuts and it may help with DNA repair or may prevent cancer cells from replicating. Keywords: Breast cancer, Dietary patterns, etiology ASSESSMENT OF CHANGES IN ISOFORMS OF MDM2 GENE BY 5FLUOROURACIL IN ESOPHAGEAL CANCER CELL LINES (KYSE-30) Ensiye Khorshidi a, Reihaneh Alsadat Mahmoudian b, Kazem Mohammadreza Abbaszadegan b, d, Mehran Anvari c, Gholamin b, *. a Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran; b Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran; c Cancer Research Center, Omid Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; d Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran * Corresponding author. E-mail address: [email protected] (M. Gholamin).
Extended Abstract Gastrointestinal cancers (GC) are prevalent cancers worldwide. Esophageal squamous cell carcinoma (ESCC) is the most common cancer in the gastrointestinal tract. Genetic factors and oncogenes have a critical role in the development of the disease. In 2008, a guesstimate 482 000 new esophageal cancer cases were recognized and 407 000 related deaths happened worldwide. A mouse double minute 2 homolog (MDM2) is a negative regulator of the p53 tumor suppressor. Mdm2 is overexpressed in some human malignant tumors. MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Transcription of the MDM2 gene is regulated by two distinct promoters (P1 and P2). It is noteworthy that, more than 40 different splice variants of MDM2 transcripts have been identified in both tumor and normal tissues. The most important three of MDM2 splice variants are Mdm2-A, Mdm2-B, and Mdm2-C. The fulllength Mdm2 protein consists of 491 amino acid. This gene is located on the chromosome location 12q13-14 and contains 12 exons. In cancer cells, Mdm2 increases growth-promoting signals and decreases cell death signals. The anti-cancer peptide PNC-27, containing the MDM2binding domain of p53, may target MDM2. This peptide by creating pores in the membrane of cancer cells induces cell death, but no in normal cells. In this study KYSE30 esophageal cancer cells were maintained in RPMI 1640 supplemented with10% FBS. For 5-FU treatments, cells were cultured in phenol red-free RPMI1640. Cells were deprived of 10% fetal bovine serum for 24h, 48h prior to drug treatment. Before and after exposure to 5-fluorouracil (Table 2), MDM2 mRNA splicing variants were examined by nested RT-PCR and sequencing. Results showed5-fluorouracil caused changes in the expression of MDM2 gene isoforms and this change is directly related to the increase in dose and time. Keywords: Cell lines KYSE-30, MDM2, Nested RT PCR, 5FU
our hospital with anorexia, diplopia, lethargy, and right leg claudication. Hodgkin's lymphoma was in remission phase for about 7 years. Six months before admission, he was admitted to hospital because of fever, night sweats, fatigue, weight loss, splenomegaly, and multiple cervical, retroperitoneal and para-aortic lymphadenopathy that brought up a presumptive diagnosis of relapsed phase of Hodgkin's disease and he received chemotherapy for 4 months. On the tenth day of hospitalization, the temperature briefly rose to 39 oC and photophobia, nuchal, passive and active foot rigidity developed. On a neurological examination, outward deviation, mydriasis, ptosis and lack of response to light were observed in the right eye. Other cranial nerves were intact. His motor strength was 5/5 in the left extremities and 4/5 in the right extremities with normal sensation. He showed dysmetria and mild hyperreflexia on the right side. Tremor on left hand with mild rigidity on left limbs was observed. Psychiatric examination also revealed depression and anxiety disorders. All necessary tests such as CBC and Serological tests, CT scan and MRI were performed. According to the basal ganglia and cerebral peduncle involvement, we suspected brain metastases arising from Hodgkin's lymphoma so the patient was candidate for brain biopsy. When the patient was treated, a prompt therapeutic response was noted. After anti-Toxoplasma therapy initiation, progression of disease was stopped, his speech was dramatically improved but motor sequelaes remained. MRI of the brain at 6 weeks after anti-toxoplasmosis therapy showed dramatically regression of the lesions, typical for toxoplasmosis. Diffuse toxoplasmosis with CNS involvement is a life-threatening disease which often avoids diagnosis so; early diagnosis of the disease has been rewarded by a rapid response to treatment. According to Centers for Disease Control and Prevention (CDC) guidelines, criteria for establishing the diagnosis of cerebral toxoplasmosis includes proper clinical features, consistent findings on imaging, positive serological studies, and a therapeutic response to treatment. All these four criteria existed in our patient. The most frequent therapy for cerebral toxoplasmosis is pyrimethamine and sulfadiazine along with folinic acid to reduce hematological complications of pyrimethamine. Our patient showed good clinical improvement within 4 days and the lesion resolved approximately within 3 weeks. A positive response to treatment can be demonstrated both clinically and radiologically.
Keywords: Nervous system, Toxoplasmosis, Hodgkin's lymphoma BREAST CANCER AND DIETARY PATTERNS Elham Dindarlou Undergraduate student. Department of Biology, Young Researchers and Elite club, Zarghan Branch, Islamic Azad University, Zarghan, Iran E-mail address: [email protected]
Extended Abstract Breast cancer is the most frequently cancer and the second cause of mortality in women. The etiology of breast cancer is multifactorial, and genetic, environmental and lifestyle factors promote the growth of cancer cells. It can occur in both men and women, but it is much more common in women. Breast cancer comprises 22.9% of invasive cancers in women and 16% of all female cancers. It is suspected that diet influences the risk of getting breast cancer. Evidence is inconclusive concerning the role of food groups, as distinct from that of major nutrients, in the etiology of breast cancer in women. Lifestyle changes have been shown to decrease breast cancer risk even in high-risk women. Measures to decrease the risk include limiting alcohol consumption, controlling body weight, being physically active, and breastfeeding. Eating a healthy diet might reduce our risk of breast cancer. A healthy diet includes plenty of vegetable, fruits, soy, green tea, antioxidant, and less fat. Women who have inherited changes in the BRCA1 and BRCA2 genes have a higher risk of breast cancer and they should get an MRI and a mammogram every year. For them exposure to radiation, such as chest Xrays, may increase the risk of breast cancer. Ovarian hormones are biological markers for breast cancer risk. Soybean consumption can be responsible in part for lower levels of ovarian
7
hormones and decreased risk of breast cancer in women. No food or diet can prevent you from getting breast cancer. But some foods can make your body the healthiest it can be, and help keep your risk for breast cancer as low as possible. Some of them are: Replace animal fats with polyunsaturated fats (in many vegetable oil) Eat more isoflavones Make sure you have enough calcium in your diet Eat plenty of fruit and vegetables Antioxidant are nutrients that can slow oxidative damage to our body's tissues. Antioxidants can help prevent breast cancer and they are found in plant foods as bright, orange carrots and purple blueberries. The most common antioxidants are vitamin A, C and E, beta carotene, selenium and lycopene. Lycopene found in red fruit and vegetables like grapefruit, watermelon and Beta-carotene found in vegetables that are orange like carrots, mango, and peach. Green tea and soy isoflavones can reduce the risk of breast and other cancers. Epidemiologic evidence suggests that vitamin D may play a role in decreasing breast cancer risk. As Antioxidants have anti-cancer properties, it recommended in daily diet. Selenium is a mineral salt that found in mushroom, spinach, beef liver, nuts and it may help with DNA repair or may prevent cancer cells from replicating. Keywords: Breast cancer, Dietary patterns, etiology ASSESSMENT OF CHANGES IN ISOFORMS OF MDM2 GENE BY 5FLUOROURACIL IN ESOPHAGEAL CANCER CELL LINES (KYSE-30) Ensiye Khorshidi a, Reihaneh Alsadat Mahmoudian b, Kazem Mohammadreza Abbaszadegan b, d, Mehran Anvari c, Gholamin b, *. a Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran; b Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran; c Cancer Research Center, Omid Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; d Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran * Corresponding author. E-mail address: [email protected] (M. Gholamin).
Extended Abstract Gastrointestinal cancers (GC) are prevalent cancers worldwide. Esophageal squamous cell carcinoma (ESCC) is the most common cancer in the gastrointestinal tract. Genetic factors and oncogenes have a critical role in the development of the disease. In 2008, a guesstimate 482 000 new esophageal cancer cases were recognized and 407 000 related deaths happened worldwide. A mouse double minute 2 homolog (MDM2) is a negative regulator of the p53 tumor suppressor. Mdm2 is overexpressed in some human malignant tumors. MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Transcription of the MDM2 gene is regulated by two distinct promoters (P1 and P2). It is noteworthy that, more than 40 different splice variants of MDM2 transcripts have been identified in both tumor and normal tissues. The most important three of MDM2 splice variants are Mdm2-A, Mdm2-B, and Mdm2-C. The fulllength Mdm2 protein consists of 491 amino acid. This gene is located on the chromosome location 12q13-14 and contains 12 exons. In cancer cells, Mdm2 increases growth-promoting signals and decreases cell death signals. The anti-cancer peptide PNC-27, containing the MDM2binding domain of p53, may target MDM2. This peptide by creating pores in the membrane of cancer cells induces cell death, but no in normal cells. In this study KYSE30 esophageal cancer cells were maintained in RPMI 1640 supplemented with10% FBS. For 5-FU treatments, cells were cultured in phenol red-free RPMI1640. Cells were deprived of 10% fetal bovine serum for 24h, 48h prior to drug treatment. Before and after exposure to 5-fluorouracil (Table 2), MDM2 mRNA splicing variants were examined by nested RT-PCR and sequencing. Results showed5-fluorouracil caused changes in the expression of MDM2 gene isoforms and this change is directly related to the increase in dose and time. Keywords: Cell lines KYSE-30, MDM2, Nested RT PCR, 5FU