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Exercise and phagocytic function: effect of exercise on human neutrophil
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4lkDa molecules on Western blots.
Seventh JADCI abstracts
Kobayashi, Kenneth Grabstein*, Raymond Paxton*, Kazuo DS. MIXED Sugamura**, LEUKOCYTE Yasunobu Yoshikai. REACTION (MLR) AND CELL- Labomtov of Host Defense & Germfnze Life, MEDIATED LYMPHOLYSIS Nagoya University School of Me&ine, (CML) IN TRIPLOID GINBUNA Nagoya 466, Jqxzn, *Immunw Research & AND TETRAPLOID GINBU NA- Development Corporation, Washington GOLDFISH HYBRID MODEL 98101, USA cotd **Deportment of SYSTEM. Teruyuki Nakani shi and Microbiology, Tohoku University, School of Mi tsuru Oto take. Notional Research Me&zinc; Se&i 980, Jqon. We have Institute of Aqaculture, Tom&i-cho, Mie previous evidence for early recruitment of y 6 519-04, Jopan. MLR and CML experiments T cells during the disease course of primary were conducted to assess whether fish were infections with Salmonella choleraesuis in able to respond to alloantigens and generate mice. Since the y 6 T cells do not, produce IL killer cells. A model system of clonal 2, the growth factor for y 6 T cells remains triploid ginbuna and tetraploid ginbuna- unknown. IL-l.5 is a novel cytokine that is goldfish hybrid was employed, where only produced by activated monocytes/ triploids could recognize allogeneic macrophages. In this study, we investigated histocompatibility antigens of tetraploids. the proliferative activity of IL15 for y6 T Triploid and tetraploid peripheral blood cells that appears after primary infection with leukocytes (PBL) were co-cultured in 96-well S. cholemesuis31N-1. y 6 T cells proliferated microtiter plates for MLR. The proliferation in the presence of recombinant IL-15 and and DNA content of cultured cells were produced appreciable levels of y -1FN and ILdetermined by FACS analysis after Quble 4. Furthermore, y 6 T cells produced y -IFN staining with FITC-conjugated anti- in response to the monocyte/macrophage cell proliferating cell nuclear antigen (PCNA) line, J774A.l infected with S. cholemesuis, mAb and propidium iodide. Vigorous which expressed an abundant level of IL-15 reactions were observed in mixed cultures of mRNA; this cytokine production was inhibited by triploid and tetraploid PBL, and triploid cells significantly anti-IL-15 antibody. Taken together, these results showed greater proliferative responses than tetraploid cells. As for CML, triploid cells suggested that IL-15 derived from infected (effecters) and tetraploid cells (targets) were macrophages may contribute to the early cultured after in vivo priming of triploids by activation of y6 T cells during salmonellosis. tetraploids. grafting the scales from EXERCISE AND PHAGOCytotoxicity was measured by an enzymatic DlO. EFFECT OF assay kit, in which LDH released upon cell CYTIC FUNCTION: ON HUMAN lysis was measured by an ELISA reader. Only EXERCISE Haruhisa Wago, sensitized triploid cells could kill target NEUTROPHIL. Naomi 1t0, Kubo ta, tetraploid cells and their killing activity Masako Haga and Kazumi increased as the effectodtarget ratio increased Mutsuko Department of Me&al PBL showed greater cytotoxic activity than Miyashita. head-kidney cells. These results provi& Technology, Soitoma Me&al School Junior evidence for the presence of alloreactive College. Soitoma 30.5-04, Jopon. When we consider the evolution of immunodefense cytotoxic T cells in teleosts. mechanisms in animals that need flight in D9. NEW GROWTH FACTOR IL- their living, it is important to investigate to 15 FOR MURINE y6 T CELLS what extent exercise has an influence on From the animal host immunodefense. INDUCED BY Salmonella Hitoshi Nishimura, phylogenetical viewpoint, this may also be INFECTION. Keuji Hiromatsu, Noli tada true for other more primitive animals. In
Seventh JAW
abstracts
this study, the effect of daily exercise such as walking on human neutrophil function was examined as a first step in phylogenetical studies. First, we examined whether exercise affected total numbers of peripheral blood leukocytes and neutrophils. Results showed that exercise increased neutrophil fiquency, but hardly affected total numbers of peripheral blood leukocytes. Furthermore, the effect on in vitro phagocytosis of yeast particles by neutrophils was analyzed revealing that exercise augmented phagocytic activity of
XIII
neutrophils. Moreover, quantification of the formazan pigment formed by the reduction of nitroblue tetrazolium revealed that the production of active oxygen was increased by exercise. IL-l production by neutrophils also showed an increase. These results clearly suggested that moderate exercise in everyday life had the enhancing effects on neutrophil numbers and functions such as phagocytosis and the production of active oxygen or IL- 1.
4lkDa molecules on Western blots.
Seventh JADCI abstracts
Kobayashi, Kenneth Grabstein*, Raymond Paxton*, Kazuo DS. MIXED Sugamura**, LEUKOCYTE Yasunobu Yoshikai. REACTION (MLR) AND CELL- Labomtov of Host Defense & Germfnze Life, MEDIATED LYMPHOLYSIS Nagoya University School of Me&ine, (CML) IN TRIPLOID GINBUNA Nagoya 466, Jqxzn, *Immunw Research & AND TETRAPLOID GINBU NA- Development Corporation, Washington GOLDFISH HYBRID MODEL 98101, USA cotd **Deportment of SYSTEM. Teruyuki Nakani shi and Microbiology, Tohoku University, School of Mi tsuru Oto take. Notional Research Me&zinc; Se&i 980, Jqon. We have Institute of Aqaculture, Tom&i-cho, Mie previous evidence for early recruitment of y 6 519-04, Jopan. MLR and CML experiments T cells during the disease course of primary were conducted to assess whether fish were infections with Salmonella choleraesuis in able to respond to alloantigens and generate mice. Since the y 6 T cells do not, produce IL killer cells. A model system of clonal 2, the growth factor for y 6 T cells remains triploid ginbuna and tetraploid ginbuna- unknown. IL-l.5 is a novel cytokine that is goldfish hybrid was employed, where only produced by activated monocytes/ triploids could recognize allogeneic macrophages. In this study, we investigated histocompatibility antigens of tetraploids. the proliferative activity of IL15 for y6 T Triploid and tetraploid peripheral blood cells that appears after primary infection with leukocytes (PBL) were co-cultured in 96-well S. cholemesuis31N-1. y 6 T cells proliferated microtiter plates for MLR. The proliferation in the presence of recombinant IL-15 and and DNA content of cultured cells were produced appreciable levels of y -1FN and ILdetermined by FACS analysis after Quble 4. Furthermore, y 6 T cells produced y -IFN staining with FITC-conjugated anti- in response to the monocyte/macrophage cell proliferating cell nuclear antigen (PCNA) line, J774A.l infected with S. cholemesuis, mAb and propidium iodide. Vigorous which expressed an abundant level of IL-15 reactions were observed in mixed cultures of mRNA; this cytokine production was inhibited by triploid and tetraploid PBL, and triploid cells significantly anti-IL-15 antibody. Taken together, these results showed greater proliferative responses than tetraploid cells. As for CML, triploid cells suggested that IL-15 derived from infected (effecters) and tetraploid cells (targets) were macrophages may contribute to the early cultured after in vivo priming of triploids by activation of y6 T cells during salmonellosis. tetraploids. grafting the scales from EXERCISE AND PHAGOCytotoxicity was measured by an enzymatic DlO. EFFECT OF assay kit, in which LDH released upon cell CYTIC FUNCTION: ON HUMAN lysis was measured by an ELISA reader. Only EXERCISE Haruhisa Wago, sensitized triploid cells could kill target NEUTROPHIL. Naomi 1t0, Kubo ta, tetraploid cells and their killing activity Masako Haga and Kazumi increased as the effectodtarget ratio increased Mutsuko Department of Me&al PBL showed greater cytotoxic activity than Miyashita. head-kidney cells. These results provi& Technology, Soitoma Me&al School Junior evidence for the presence of alloreactive College. Soitoma 30.5-04, Jopon. When we consider the evolution of immunodefense cytotoxic T cells in teleosts. mechanisms in animals that need flight in D9. NEW GROWTH FACTOR IL- their living, it is important to investigate to 15 FOR MURINE y6 T CELLS what extent exercise has an influence on From the animal host immunodefense. INDUCED BY Salmonella Hitoshi Nishimura, phylogenetical viewpoint, this may also be INFECTION. Keuji Hiromatsu, Noli tada true for other more primitive animals. In
Seventh JAW
abstracts
this study, the effect of daily exercise such as walking on human neutrophil function was examined as a first step in phylogenetical studies. First, we examined whether exercise affected total numbers of peripheral blood leukocytes and neutrophils. Results showed that exercise increased neutrophil fiquency, but hardly affected total numbers of peripheral blood leukocytes. Furthermore, the effect on in vitro phagocytosis of yeast particles by neutrophils was analyzed revealing that exercise augmented phagocytic activity of
XIII
neutrophils. Moreover, quantification of the formazan pigment formed by the reduction of nitroblue tetrazolium revealed that the production of active oxygen was increased by exercise. IL-l production by neutrophils also showed an increase. These results clearly suggested that moderate exercise in everyday life had the enhancing effects on neutrophil numbers and functions such as phagocytosis and the production of active oxygen or IL- 1.