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Growth inhibitory activities of Siegesbeckiae glabrescens against foodborne pathogens
Abstracts / Journal of Biotechnology 136S (2008) S717–S742
VIII1-P-045 Growth inhibitory activities of Siegesbeckiae glabrescens against foodborne pathogens Chang-Ho Kang 1,∗ , Choe-Rong Hong 1 , Hye-Sung Youm 1 , Soo-Im Choi 2 , Tae-Ryeon Heo 1 1 Department of Biological Engineering, Inha University, 402-751, Incheon, South Korea 2 Plant Resources Research Institute, Duksung Women’s University, Seoul 132-714, South Korea
E-mail address: [email protected] (C.-H. Kang). In order to research for anti-microbial formation agents from Korean medicinal herbs, we selected 16 Korean medicinal herbs. We tested for growth inhibition effect of 16 medicinal herb ethanolic extracts on foodborne microorganisms (Escherichia coli O157:H7, Staphylococcus aureus ATCC 25923, Salmonella typhimurium KCTC 2514, Bacillus subtilis KCTC 3716, and Bacillus cereus KCTC 3711). The antimicrobial activities of these extracts were assessed using discdiffusion and turbidity assays. Among tested extracts, Siegesbeckiae glabrescens (SG) ethanolic extract showed the strongest sensitivity on all tested pathogens. The inhibition zones of SG (1000 g/mL) against S. aureus, B. cereus, and B. subtilis were 12, 13 and 16.5 mm, respectively. In addition, SG (100–1000 ppm) showed a high growth inhibitory activity of 62–100 ± 7.8% against S. aureus and B. cereus and B. subtilis in the turbidity assay. The n-hexane solvent fractionates (100–1000 ppm) from ethanolic extracts of SG displayed strong growth inhibitory activity 92–100% against S. aureus, B. cereus and B. subtilis. Therefore, SG could be useful as an antimicrobial agent against foodborne pathogens.
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of PKSs in those fungal strains including Aspergillus species using PCR amplification and bioinformatics. Diverse ketosynthase (KS) genes were retrieved from the fungal community associated with the Korean traditional fermented food (doenjang), especially meju which is main soybean substrate of doenjang. We identified 23 different fungal strains from Sunchang meju by restriction fragment length polymorphism (RFLP) method using ITS sequence analyses. With four restriction endonucleases (AluI, HaeIII, HhaI, TaqI), a specific restriction pattern was yielded and Aspergillus oryzae, Aspergillus fumigatus, Fusarium asiaticum, Aspergillus sydowii, and Arthrinium sp. were identified by sequence analysis. Using degenerate PCR primers designed with conserved regions for fungal KS domain in polyketide synthase (PKS), about 700 bp of various amplified DNA fragments were detected. Altogether 10 different putative KS domain sequences were identified. Those KS domain sequences were aligned and analyzed by phylogenetic methods. The KS domain sequences were significantly diverse implying that they most probably represent PKSs responsible for different functions in fungal strains. References Bingle, L.E.H., Simpson, T.J., Lazarus, C.M., 1999. Ketosynthase domain probes identify two subclasses of fungal polyketide synthase. Genes Fungal Genet. Biol. 26, 209–223. Martinez-Culebras, P.V., Ramon, D., 2007. An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine. Int. J. Food Microbiol. 113, 147–153. Nicholson, T.P., Rudd, B.A.M., Dawson, M., Lazarus, C.M., Simpson, T.J., Cox, R.J., 2001. Design and utility of oligonucleotide gene probes for fungal polyketide synthases. Chem. Biol. 8, 157–178.
doi:10.1016/j.jbiotec.2008.07.1746 References Batt, C., Solberg, M., Ceponis, M. Effect of volatile components of carrot seed oil on growth and aflatoxin production by Aspergillus parasiticus. J. Food Sci. 48, 762–766. Branen, J., Davidson, P.M. Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of EDTA. Lett. Appl. Microbiol. 30, 233–237. Payne, K.D., Davidson, P.M., Oliver, S.P., Christen, G.L. Influence of bovine lactoferrin on the growth of Listeria monocytogenes. J Food Prot. 53, 468–472.
doi:10.1016/j.jbiotec.2008.07.1745 VIII1-P-046 Diversity of polyketide synthase genes associated with fungal strains found in Korean traditional food Jong-Hyun Jung 1,∗ , Dong-Ho Seo 1 , Suk-Jin Ha 1 , Jong-Sang Kim 2 , Jeong Hwan Kim 3 , Dae Young Kwon 4 , Jaeho Cha 5 , Cheon-Seok Park 1 1 Department of Food Science and Biotechnology, KyungHee University, Yongin 449-701, Republic of Korea 2 Department of Animal Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea 3 Department of Food Science and Technology, Gyeongsang National University, Jinju 660-701, Republic of Korea 4 Korea Food Research Institute, Sungnam 463-746, Republic of Korea 5 Department of Biological Science, Pusan National University, Busan 609-735, Republic of Korea
Fungal polyketide synthases (PKSs) are responsible for the biosynthesis of many secondary metabolites and several mycotoxins. The purpose of this study was to investigate the fungal strains in the Korean traditional fermented food, and to examine the diversity
VIII1-P-047 Effect of polyethylenimine and glutaraldehyde on palatinose production by immobilized Enterobacter SP. FMB1 Jong-Yul Park 1 , Dong-Ho Seo 1 , Jong-Hyun Jung 1 , Suk-Jin Ha 1 , Jaeho Cha 2 , Cheon-Seok Park 1,∗ 1 Department of Food Science and Biotechnology, KyungHee University, Yongin 449-701, Republic of Korea 2 Department of Microbiology, Pusan National University, Pusan 609735, Republic of Korea
Palatinose (6-O-a-d-glucopyranosyl-d-fructose) is the structural isomers of sucrose and produced naturally by some microorganisms as a reserve material during periods of low carbon availability (Lina et al., 2002). Recently, a palatinose-producing microorganism identified as Enterobacter sp. FMB1 was isolated from traditional Korean foods, Meju (Cho et al., 2007). As an ideal palatinose production for industrial use would exhibit high conversion yield and rate, high specificity, and a wide window of reaction conditions, packed bed reactor method was examined by using immobilized Enterobacter sp. FMB1 cells. To increase the stability of immobilized cell, polyethylenimine and glutaraldehyde were applied. With the addition of polyethylenimine (2%) and glutaraldehyde (0.3%), half-life of immobilized cell increased three-fold. Interestingly, the conversion rate from sucrose to palatinose was significantly affected by the concentration of Mn2+ . With Mn2+ (5 mM), conversion rate and half-life of immobilized cell increased 13% and 18%, respectively. References Cho, M.H., Park, S.E., Lim, J.K., Kim, J.S., Kim, J.H., Kwon, D.Y., Park, C.S., 2007. Conversion of sucrose into isomaltulose by Enterobacter sp. FMB1, an
VIII1-P-045 Growth inhibitory activities of Siegesbeckiae glabrescens against foodborne pathogens Chang-Ho Kang 1,∗ , Choe-Rong Hong 1 , Hye-Sung Youm 1 , Soo-Im Choi 2 , Tae-Ryeon Heo 1 1 Department of Biological Engineering, Inha University, 402-751, Incheon, South Korea 2 Plant Resources Research Institute, Duksung Women’s University, Seoul 132-714, South Korea
E-mail address: [email protected] (C.-H. Kang). In order to research for anti-microbial formation agents from Korean medicinal herbs, we selected 16 Korean medicinal herbs. We tested for growth inhibition effect of 16 medicinal herb ethanolic extracts on foodborne microorganisms (Escherichia coli O157:H7, Staphylococcus aureus ATCC 25923, Salmonella typhimurium KCTC 2514, Bacillus subtilis KCTC 3716, and Bacillus cereus KCTC 3711). The antimicrobial activities of these extracts were assessed using discdiffusion and turbidity assays. Among tested extracts, Siegesbeckiae glabrescens (SG) ethanolic extract showed the strongest sensitivity on all tested pathogens. The inhibition zones of SG (1000 g/mL) against S. aureus, B. cereus, and B. subtilis were 12, 13 and 16.5 mm, respectively. In addition, SG (100–1000 ppm) showed a high growth inhibitory activity of 62–100 ± 7.8% against S. aureus and B. cereus and B. subtilis in the turbidity assay. The n-hexane solvent fractionates (100–1000 ppm) from ethanolic extracts of SG displayed strong growth inhibitory activity 92–100% against S. aureus, B. cereus and B. subtilis. Therefore, SG could be useful as an antimicrobial agent against foodborne pathogens.
S733
of PKSs in those fungal strains including Aspergillus species using PCR amplification and bioinformatics. Diverse ketosynthase (KS) genes were retrieved from the fungal community associated with the Korean traditional fermented food (doenjang), especially meju which is main soybean substrate of doenjang. We identified 23 different fungal strains from Sunchang meju by restriction fragment length polymorphism (RFLP) method using ITS sequence analyses. With four restriction endonucleases (AluI, HaeIII, HhaI, TaqI), a specific restriction pattern was yielded and Aspergillus oryzae, Aspergillus fumigatus, Fusarium asiaticum, Aspergillus sydowii, and Arthrinium sp. were identified by sequence analysis. Using degenerate PCR primers designed with conserved regions for fungal KS domain in polyketide synthase (PKS), about 700 bp of various amplified DNA fragments were detected. Altogether 10 different putative KS domain sequences were identified. Those KS domain sequences were aligned and analyzed by phylogenetic methods. The KS domain sequences were significantly diverse implying that they most probably represent PKSs responsible for different functions in fungal strains. References Bingle, L.E.H., Simpson, T.J., Lazarus, C.M., 1999. Ketosynthase domain probes identify two subclasses of fungal polyketide synthase. Genes Fungal Genet. Biol. 26, 209–223. Martinez-Culebras, P.V., Ramon, D., 2007. An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine. Int. J. Food Microbiol. 113, 147–153. Nicholson, T.P., Rudd, B.A.M., Dawson, M., Lazarus, C.M., Simpson, T.J., Cox, R.J., 2001. Design and utility of oligonucleotide gene probes for fungal polyketide synthases. Chem. Biol. 8, 157–178.
doi:10.1016/j.jbiotec.2008.07.1746 References Batt, C., Solberg, M., Ceponis, M. Effect of volatile components of carrot seed oil on growth and aflatoxin production by Aspergillus parasiticus. J. Food Sci. 48, 762–766. Branen, J., Davidson, P.M. Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of EDTA. Lett. Appl. Microbiol. 30, 233–237. Payne, K.D., Davidson, P.M., Oliver, S.P., Christen, G.L. Influence of bovine lactoferrin on the growth of Listeria monocytogenes. J Food Prot. 53, 468–472.
doi:10.1016/j.jbiotec.2008.07.1745 VIII1-P-046 Diversity of polyketide synthase genes associated with fungal strains found in Korean traditional food Jong-Hyun Jung 1,∗ , Dong-Ho Seo 1 , Suk-Jin Ha 1 , Jong-Sang Kim 2 , Jeong Hwan Kim 3 , Dae Young Kwon 4 , Jaeho Cha 5 , Cheon-Seok Park 1 1 Department of Food Science and Biotechnology, KyungHee University, Yongin 449-701, Republic of Korea 2 Department of Animal Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea 3 Department of Food Science and Technology, Gyeongsang National University, Jinju 660-701, Republic of Korea 4 Korea Food Research Institute, Sungnam 463-746, Republic of Korea 5 Department of Biological Science, Pusan National University, Busan 609-735, Republic of Korea
Fungal polyketide synthases (PKSs) are responsible for the biosynthesis of many secondary metabolites and several mycotoxins. The purpose of this study was to investigate the fungal strains in the Korean traditional fermented food, and to examine the diversity
VIII1-P-047 Effect of polyethylenimine and glutaraldehyde on palatinose production by immobilized Enterobacter SP. FMB1 Jong-Yul Park 1 , Dong-Ho Seo 1 , Jong-Hyun Jung 1 , Suk-Jin Ha 1 , Jaeho Cha 2 , Cheon-Seok Park 1,∗ 1 Department of Food Science and Biotechnology, KyungHee University, Yongin 449-701, Republic of Korea 2 Department of Microbiology, Pusan National University, Pusan 609735, Republic of Korea
Palatinose (6-O-a-d-glucopyranosyl-d-fructose) is the structural isomers of sucrose and produced naturally by some microorganisms as a reserve material during periods of low carbon availability (Lina et al., 2002). Recently, a palatinose-producing microorganism identified as Enterobacter sp. FMB1 was isolated from traditional Korean foods, Meju (Cho et al., 2007). As an ideal palatinose production for industrial use would exhibit high conversion yield and rate, high specificity, and a wide window of reaction conditions, packed bed reactor method was examined by using immobilized Enterobacter sp. FMB1 cells. To increase the stability of immobilized cell, polyethylenimine and glutaraldehyde were applied. With the addition of polyethylenimine (2%) and glutaraldehyde (0.3%), half-life of immobilized cell increased three-fold. Interestingly, the conversion rate from sucrose to palatinose was significantly affected by the concentration of Mn2+ . With Mn2+ (5 mM), conversion rate and half-life of immobilized cell increased 13% and 18%, respectively. References Cho, M.H., Park, S.E., Lim, J.K., Kim, J.S., Kim, J.H., Kwon, D.Y., Park, C.S., 2007. Conversion of sucrose into isomaltulose by Enterobacter sp. FMB1, an