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HPLC in Nucleic Acids Research: Methods and Applications, Chromatographic Science Series, Vol. 28
351
Phyllis R. Brown (Ed.), HPLC in Nucleic Acids Research: Methods and Applications, Chromatogmphic Science Series, Vol. 28. Marcel Dekker, Inc., New York and Basel, 1984 (ISBN O-8247-7236-9). pp. 424. Price $71.50. With the ever growing interest in genetic engineering, nucleotide and nucleoside synthesis, and effecting metabolic controls there is a definite need for a text, usable as a practical laboratory guide, which also provides an up-to-date, concise and easily digestable review of separative methods in the field of nucleic acid research. In many respects this book fulfils these requirements. The book’s contents are suitably arranged in three parts: overview, methodology and applications. The subsections are logically set out, providing a basis at each stage for progression to the next and allowing information to be readily accessible. As an overview, Part I is somewhat limited since it attempts to cover whole subject areas in just a few sentences. This provides the informed (perhaps?) with merely an elementary reminder and the uninitiated with rather a bare skeletal outline. The methodology of nucleic acid chemistry presented in Part II still tries to cover large topic areas too briefly, with the result that only the basics of each technique are described. However, most subject areas are well supported by a useful list of references. Since the title of the book implies an emphasis on specialism, it would have perhaps been better to assume a basic understanding of the more commonly known methods and give, for example, a more detailed description of the chromatographic methods. The applications outlined in Part III should prove very useful to workers in the areas the book hopes to attract. Many helpful practical tips are given and the range of examples chosen is comprehensive, well documented and related to problems of current interest in the field. A minor criticism is that there is some repetition of the general techniques outlined in Part II. Maybe the book is taking too much on in too short a space to satisfy both the novice and experienced researcher, but despite this it is likely to prove a useful asset to those working in the field of nucleic acids research. G. Mackenzie R. W. Humble
E. Stahl and W. Schild, Isolierung und Characterisierung uon Naturstoffen. G. Fischer, Stuttgart, 1986 (ISBN 3-437-30511-5). pp. x + 180. Price DM 39.80. This excellent and (2) Isolation section comprises main methods but sublimation and
small text book is divided into two sections: (1) Methods and Characterisation of Natural Products. The methods outlines of extraction procedures, chromatography (all with detailed emphasis on adsorption thin-layer methods), spectroscopic methods (u.v.-visible, infrared), including
Phyllis R. Brown (Ed.), HPLC in Nucleic Acids Research: Methods and Applications, Chromatogmphic Science Series, Vol. 28. Marcel Dekker, Inc., New York and Basel, 1984 (ISBN O-8247-7236-9). pp. 424. Price $71.50. With the ever growing interest in genetic engineering, nucleotide and nucleoside synthesis, and effecting metabolic controls there is a definite need for a text, usable as a practical laboratory guide, which also provides an up-to-date, concise and easily digestable review of separative methods in the field of nucleic acid research. In many respects this book fulfils these requirements. The book’s contents are suitably arranged in three parts: overview, methodology and applications. The subsections are logically set out, providing a basis at each stage for progression to the next and allowing information to be readily accessible. As an overview, Part I is somewhat limited since it attempts to cover whole subject areas in just a few sentences. This provides the informed (perhaps?) with merely an elementary reminder and the uninitiated with rather a bare skeletal outline. The methodology of nucleic acid chemistry presented in Part II still tries to cover large topic areas too briefly, with the result that only the basics of each technique are described. However, most subject areas are well supported by a useful list of references. Since the title of the book implies an emphasis on specialism, it would have perhaps been better to assume a basic understanding of the more commonly known methods and give, for example, a more detailed description of the chromatographic methods. The applications outlined in Part III should prove very useful to workers in the areas the book hopes to attract. Many helpful practical tips are given and the range of examples chosen is comprehensive, well documented and related to problems of current interest in the field. A minor criticism is that there is some repetition of the general techniques outlined in Part II. Maybe the book is taking too much on in too short a space to satisfy both the novice and experienced researcher, but despite this it is likely to prove a useful asset to those working in the field of nucleic acids research. G. Mackenzie R. W. Humble
E. Stahl and W. Schild, Isolierung und Characterisierung uon Naturstoffen. G. Fischer, Stuttgart, 1986 (ISBN 3-437-30511-5). pp. x + 180. Price DM 39.80. This excellent and (2) Isolation section comprises main methods but sublimation and
small text book is divided into two sections: (1) Methods and Characterisation of Natural Products. The methods outlines of extraction procedures, chromatography (all with detailed emphasis on adsorption thin-layer methods), spectroscopic methods (u.v.-visible, infrared), including